MOC 기초연구실 정기세미나
주관: 인하대학교 바이오융합시스템 연구실 후원: 한국연구재단 기초연구실 사업
지원: 인하대학교 바이오시스템융합학과
1997~2004 서울대학교 응용화학부(현 화학생물공학부) 화학공학 학사
제 5 회
2021.12.29.일 오전 10시
인하대학교 온라인 강연
최낙원 박사 / KIST
제목 초록
약력
2003~2004 한국과학기술연구원(KIST) 위촉연구원
2010~2011 Novartis Institutes for Biomedical Research (NIBR), Lead Finding Platform (LFP), Presidential Postdoctoral Fellow / Massachusetts Institute of
Technology (MIT), Department of Chemical Engineering, Research Affiliate 2012~현재 한국과학기술연구원(KIST) 뇌과학연구소 선임연구원/책임연구원
2019~현재 고려대학교 KU-KIST 융합대학원 학연교수
경력
Hydrogel-based bioassay developments for in vitro diagnosis and prognosis
We introduce developments of hydrogel-based bioassays by UV-crosslink of polyethylene glycol diacrylate (PEGDA) microparticles or microposts. The first example presents hydrogel micropost-based qPCR for multiplex detection of miRNAs associated with Alzheimer's disease (AD). Acrylated forward primers incorporated in polyethylene glycol diacrylate (PEGDA) posts played a crucial role in confining fluorescent signals of cDNA amplification within the PEGDA hydrogel. To demonstrate the potential of our platform, we successfully verified multiplex detection of five miRNAs, which were reported to be highly correlated with AD, from a complex buffer of human plasma. The second example shows hydrogel-based hybridization chain reaction (HCR) for multiplex signal amplification to detect urinary exosomal miRNAs from human clinical samples. We identified small amounts (~amol) of exosomal miRNAs from 600 μL of urine with up to ~35-fold amplification and enhanced detection limits by over an order of magnitude for two miRNA biomarker candidates: hsa-miR-6090 and hsa-miR-3665.
문의처: [email protected] 032-867-7328
2004~2010 Cornell University, School of Chemical and Biological Engineering,
화학공학 석사, 박사
MOC 기초연구실 정기세미나
주관: 인하대학교 바이오융합시스템 연구실 후원: 한국연구재단 기초연구실 사업
지원: 인하대학교 바이오시스템융합학과
2003~2008 (B.S.)Seoul National University Mechanical and Aerospace Engineering
제 5 회
2021.12.29.일 오전 10시 30분
인하대학교 온라인 강연
김홍남 박사 / KIST
제목 초록
약력
2014~2015 (Post-doc)Korea Institute of Science and Technology, Brain Science Institute 2016~ (Sr. Researcher)Korea Institute of Science and Technology, Brain Science Institute 2016~ (Assistant and Associate Professor) UST, Division of Bio Med
2021~ (Adjunct Professor) Yonsei University, Mechanical Engineering
경력
Brain-on-a-Chip Technology for
Mimicking Brain Microvasculature Physiology and Pathology
We present a brain-on-a-chip platform in which multiple types of human brain-originated brain cells were cocultured in a 3D microenvironment with perfusable brain microvasculature.
The brain microvasculature was fabricated by using microneedles as templates. The removal of microneedles after the gelation of the collagen matrix allowed the formation of collagen channels with the identical diameter of microneedles. By seeding the brain microvascular endothelial cells in the luminal surface of the channel and embedding other neural cells in the collagen, a 3D brain model was prepared. The brain microvasculature physiology was confirmed through transendothelial permeability, immunofluorescence imaging, and cytokine assay. Neuropathology such as brain tumor, and particulate matter-induced neuroinf
ammation were recapitulated in the brain-on-a-chip platform. Pathophysiological features as well as the efficacy of pharmaceutical candidates were evaluated in the platforms. We believe that the brain-on-a-chip technology can not only help the unraveling of underlying
mechanisms of brain diseases but also testing the efficacy of potential pharmaceutic candidates.
문의처: [email protected] 032-867-7328
2008~2010 (M.S.)Seoul National University Mechanical and Aerospace Engineering 2010~2014 (ph.D.)Seoul National University Mechanical and Aerospace Engineering
