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Rhizobium galegae Galega orientalis // . 2000.
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2. ., .,
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-
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-
» 3-6 2003 . ., 2003.
3. . -
-
: . . , 2005.
4. // . . . -
. .: , 2005. 608 .
5. ., . -
. . .: , 2002. 589 .
6.Deisingh F.K., Tompson M. Biosensors for the detection of bacteria // Canadian Journal of Microbiology. 2004. V. 50.
P. 69-77.
7.Wilson K. Molecular techniques for the study ofRhizo- bial ecology in the field // Soil Biology and Biochemistry. 1995.
V. 27, N5. P. 501-512.
8. . -
Chlamyd omonas reinhardtii
// . .
. . 2002. 2. . 90-93.
9.Ercole C., Del Gallo M., Pantalone M. et al. A biosensor forE.colibased on a potentoometric alternating biosensing transduser // Sens. Actuators. 2002. V. 83. P. 48-52.
for fast detection of pathogenic bacteria // Elecrtochem.
Commun. 2000. V. 2. P. 457-460.
11.Chang A.C., Gillespie J.B., Tabacco M.B. Enchanced detection of live bacteria using a dendrimer thin film in an optical biosensor // Anal. Chem. 2001. V. 73. P. 467-470.
12.Chan S., Horner S.R, Fauchet P.M., Miller B.L. Identi- fication of Gram negative bacteria using nanoscale silicon microcavities // J. Am. Chem. Soc. 2001. V. 123. P. 11797- 11798.
13.Olsen P.E., Sande E.S., Keyser H.H. et al. A very rapid enzyme immunoassay for confirmation of Rhizobial identity and estimation of cell numbers in fresh broth culture // Can.
J. Microbiol. 1998. V. 44. P. 380-385.
14. Wang Ch., Wang D., Zhou Qi. Colonization and persistence of a plant growth-promoting bacteriumPseudomo- nas fluorescens strain CS 85, on roots of cotton seedlings //
Can. J. Microbiol. 2004. V. 50. P. 475-481.
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i i, i
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Summary
In the review general-theoretical bases and individual aspects of the problem of indication, activity and survival of the microorganisms introduced in the Soils are considered.
579: 574; 579.64: 631.46
, . 19.04.06 .