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UNIVERSITI TEKNOLOGI MARA

DEVELOPMENT CYCLE OF SELECTED STRAINS OF AEDES AEGYPTI (LINNAEUS) BASED ON WATER TEMPERATURE

AND TYPE OF CONTAINERS

FARAH IZZATI BINTI MOHAMMAD SABRI

Project submitted in fulfillment of the requirements for the degree of

Bachelor in Environmental Health and Safety (Hons.)

Faculty of Health Sciences

July 2017

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ii

DECLARATION BY STUDENT

Project entitled “Development cycle of selected strains of Aedes aegypti (Linnaeus) based on water temperature and type of containers” is a presentation of my original research work. Whenever contributions of others are involved, every effort is made to indicate this clearly, with due reference to literature, and acknowledgement of collaborative research and discussions. The project was done under the guidance of Project Supervisor, Dr. Nazri bin Che Dom. It has been submitted to the Faculty of Health Sciences in partial fulfilment of the requirement for the Degree of Bachelor in Environmental Health and Safety (Hons).

Student’s signature:

…………...

(Farah Izzati Bt Mohammad Sabri) 2014842436

930615-03-5500 Date: July 2017

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vi

ACKNOWLEDGEMENT

In the name of Allah, The Most Gracious, The Most Merciful.

Assalamualaikum and Alhamdulillah, all praise to Allah S.W.T The Supreme Lord of the Universe. Peace and blessing to Nabi Muhammad S.A.W., all prophets and their families. I praise Allah S.W.T. for the strength and His blessings in completing my study.

Thousands of thanks and love to my parents Mr.Mohammad Sabri Jusoh and Mrs. Asmayati Deraman for their support and encouragement through thick and thin of my study. A lot of thanks also credited to my husband, Ahmad Fauzan Radzuan for his continuous support. My deepest gratitude and appreciation to my dearest supervisor, Dr. Nazri Bin Che Dom who spent his time and efforts in guiding and advising from the beginning till the end of my research journey. Not to forget, I would like to thank all the lecturers in Department of Environmental Health and Safety, Faculty of Health Sciences who always share their thoughts, knowledge and advice throughout my study in UiTM Puncak Alam. Only God can reward all of you with goodness.

My sincere thanks and appreciation goes to all the staff from the department, insectarium and laboratory who gave their full cooperation and assisted me in many ways throughout my study. A special thanks to my friends from HS243 especially my partner, Alicia Mindy Rogie who always gives me support and motivation while completing my study. May our friendship last forever. Lastly, I would like to thank everyone who involved directly and indirectly in this study. Thank You.

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vii

TABLE OF CONTENTS

TITLE PAGE

DECLARATION BY STUDENT ii

INTELLECTUAL PROPERTIES iii

APPROVAL BY SUPERVISOR v

ACKNOLEDGEMENT vi

TABLE OF CONTENT vii

LIST OF FIGURE x

LIST OF TABLE xii

LIST OF PLATE xiii

LIST OF APPENDICES xiv

ABSTRACT xv

ABSTRAK xvi

CHAPTER 1 : INTRODUCTION

1.1 Background of the study 1

1.2 Problem statement 3

1.3 Study objective 5

General objective 5

Specific objective 5

1.4 Hypothesis 5

1.5 Scope and limitation of the study 6

1.6 Study justification 8

1.7 Conceptual framework 9

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xv

ABSTRACT

Vector borne diseases has been partially associated with the current changes of global warming which can be included water temperatures and type of containers.

This study was conducted to determine the effect of increase water temperature on the development of Aedes aegypti immatures under laboratory condition using temperature regulated water bath to cover a range of temperature from 25°C to 35°C.

Two experiments were designed: (1) for local strain of Aedes aegypti (SEL-strain, JHR-strain, and SBH-strain) and four types of container under setting

temperature regimes. The containers that were collected are tire, plastic, glass and coconut shell. The experiments were conducted under presence of larvae food.

The analysis is based on observation of the immature stage development (days) in

response to different water temperatures and type of containers.

The general profile of the larval development based on water temperatures and container’s type were tabulated using mean number. The larval development to pupa was the fastest when the water temperatures increasing and also led by coconut shell in term of water container. In conclusion, the water temperature and type of containers were significantly (p-value < 0.05) affect the larval development days.

This study concludes that the knowledge on the larval development’s factor is important in dengue control especially on the larval development days of Ae. aegypti.

Keywords: Aedes aegypti, water temperatures, immature stages, container’s type, pupae emergence, development days

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