VASCULAR CELL WORKSHOP:
Registration deadline: 16 th AUGUST 2019
Tissue Engineering Centre, UKMMC
Primary cell culture & its application
27
TH- 29
THAUGUST 2019
https://goo.gl/forms/dB HtAWfz30d1UFco2
REGISTRATION FORM
LIMITED 16 SEATS ONLY
For further information, please contact:
Tel: 0391457158; Fax: 0391457678; E-mail: [email protected] Dr Nadiah Sulaiman ( +603-91457684; [email protected] )
Sponsored by:
REGISTRATION FEE
Categories Student Professional
Day 1 – Day 3 (27
thtill 29
thAugust 2019)
Seminar & hands-on RM850 RM980 Day 1 – Day 4 (27
thtill 30
thAugust 2019)
Additional hands-on training “Fluorescence and Confocal Microscopy” RM1000 RM1200
VASCULAR CELL WORKSHOP:
Primary cell culture & its application
Day 1 SEMINAR: 27 AUGUST 2019 (TUESDAY)
8:00 am Registration & breakfast8:30 am Opening ceremony & Welcome Speech by Head of Tissue Engineering Centre (TEC), UKMMC
9:00 am LECTURE 1: Primary cell isolation, culture and maintenance (Dr Dain; TEC,UKM) 10:00 am LECTURE 2: Cell characterization techniques and imaging (PM Dr Angela; TEC,UKM) 11:30 am Lab tour to TEC
12:00 pm DEMO: Aseptic techniques & Preparation of buffers, culture media and related reagents
1:00 pm Lunch Talk ‘Basics about 3D cell culture and advantage of this technique for compound/ drug testing’
by Dr Zinnia the Scientific Application Manager at PromoCell 1:30 pm Lunch Break
2:00 pm HANDS-ON 1: Tissue processing (EC and SMC isolation from blood vessel) 5:00 pm End of Day 1
Day 2: 28 AUGUST 2019 (WEDNESDAY)
8:30 am Breakfast9:00 am LECTURE 3: Application & testing of cell & tissue culture (Dr Nadiah; TEC,UKM)
10:00 am LECTURE 4: Vascular cells population: What can we do with them? (Dr Najmi; FSK,UKM) 11:00 am HANDS-ON 2: Cell trypsinization for serial culture & Cryopreservation
1:00 pm Lunch Talk sponsored by SPD Scientific 1:30 pm Lunch Break
2:00 pm HANDS-ON 3: Cryopreserved cell thawing and seeding & Culture maintenance 4.00 pm PRACTICE 4: MTT Assay, Migration Assay, Tube forming Assay preparation and set-up 5:00 pm End of Day 2
Day 3: 29 AUGUST 2019 (THURSDAY)
8:30 am Breakfast9:00 am HANDS-ON 4:
1. MTT Assay 2. Migration Assay 3. Tube forming Assay 4. Image Analysis
1:00 pm Lunch Talk sponsored by Interscience (ITS) 1:30 pm Lunch Break
2:00 pm SPONSOR MARKETING TALK 3.00 pm Discussion Session and closing 4.00 pm End of Workshop
