Annex A CBC Floor Plan, Initial Layout

(1)

CBC

LABORATORY

(2)

(3)

GROUND FLOOR

GROWTH CHAMBER

ANTEROOM

STORAGE

DNA EXTRACTION ROOM

ISOLATION RROM

(BACTERIA & FUNGI) ^DECONTAMROOM

PCR PREPARATION ROOM

PCR ROOM

ELECTROPHORESIS/

GEL DOCUMENTATION ROOM

PHENOTYPING ROOM FREEZER ROOM

WASH ROOM

UTILITY ROOM

OFFICE

DATA ROOM/

BIOINFORMATICS

H A L L W A Y 1

3.78 m 2 m 5.78 m

ANTEROOM

2.4 m 1 m

H A L L W A Y 2

H A L L W A Y 3

(4)

0.13 m

4 3

3-door MEDICOOL

Ground Floor - PCR Prep Room

sink

1

sink

1 2

Ice maker

6

Laminar flow hood

-20 freeze

r/ref

autoc lave

Drying oven

5

1.3 m 11.56 m

7.9 m

LEGEND:

- Electrical outlet (2 gang) - Water outlet and drain

- no backrest chair/stool - entrance (no door)

- sliding door

(5)

Ground Floor – PCR Room & Gel Doc Room

13

14

9

10

^{5.71 m}

5.78 m

EQUIPMENT IN ROOM

• AGE

• PAGE

• GELDOC

• SONICATOR

• Heating plate

• Bottletop dispenser

• Benchtop ph meter

11

ref

sink

1m

2.19 m

LEGEND:

- no backrest chair/stool - entrance (no door) - sliding door

Other Notes:

• Wall – 0.13m thick (assuming)

• Electric outlets about 1m apart

(6)

Ground Floor – DNA Extraction Area

16

5.78 m

8.4 m

1.32 m

17

Fumehood fumehood

Geno 0.5m Geno 0.5m

18

Lyophi 0.5m

SINK

EQUIPMENT IN ROOM

•Fumehood - 2

•Lyophilizer - 2

•Genogrinder - 2

•Waterbath 2

•Microcentrifuge (2 refrigerated)

•Microcentrifuge (2 non-refrigerated)

•Vortex -3

•Thermomixer -1

•Rotary evaporator -1

•Bottletop dispenser, 0.2-2ml – 2

•BENCHTOP/mini microcentrifuge -2

•Vacuum concentrator 1

19

20

17

1.2 m 0.7 m 1.2 m

fumehood

LEGEND:

- no backrest chair/stool - two-way swing door

Other Notes:

• Chemical storage cabinet – stores empty chemical bottles, working stock

(chemicals), 1 – irritants 2 – corrosives

21

(7)

incubator Shaking

incubato r

2-door Medicool

Proposed Ground Floor – Micro and Viro Lab

sink

27

VIROLOGY ROOM 2 m

2.4 m

1.5 m

3 m

1 m

0.6 m

1.6 m

6.4 m

11.56 m

autoclave

25 23 24

Ultrahigh centrifuge

autoclave

sink

Clean

28

bench Biosafety

cabinet

Incubat or

26

Ref/freezer

Incubat or

1.62m

1 m

LEGEND:

- no backrest chair/stool - sliding door

Other Notes:

(8)

Ground Floor – Phenotyping Room

33

5.7 m

sink

34

freezer no frost

5.43 m

LEGEND:

Other Notes:

(9)

Ground Floor – Wash Room and Freezer Room

Liquid N maker

4 m

8 m

5.43 m

Water purification

sink sink

-80 -80 -80

-20 -20 -20 -20

freezer no frost

LEGEND:

Other Notes:

• Room should have strong exhaust and good ventilation

• Room should have AC unit

38

39

(10)

Ground Floor – Storage

4-layer shelf

LEGEND:

- Electrical outlet (2 gang)

Other Notes:

5.4 m

5.43 m

(11)

(12)

SECOND FLOOR

GTL DARK ROOM

GTL PREPARATION

ROOM

OFFICE H A L L W A Y

GTL ISOLATION

ROOM

ELECTROPHORESIS/

GEL DOCUMENTATION ROOM

GTL LIGHT ROOM

COMMON EQUIPMENT ROOM

SEQUENCING ROOM

TCL LIGHT ROOM

TCL DARK ROOM TCL

PREPARATION ROOM WASH ROOM

TCL ISOLATION

ROOM

UTILITY ROOM

(13)

Second Floor – GTL Prep Room

FUMEHOOD

43

REF

46

44

sink

44

11.05 M

5.05 M

6.0 M

5.76 M

47 45

42

autoclave autoclave

1.3 M

Ice maker

46 48

LEGEND:

- Electrical outlet (2 gang) - Water outlet and drain

- no backrest chair/stool - 2-way swing door

Other Notes:

• Wall – 0.13m thick (assuming)

• Electric outlets about 1m apart

(14)

Second Floor – GTL Dark and Light Room

Growth Chamber Rooms

5.75 m

5.05 m

5.76 m

TC Chambe

r

TC Chambe

r

TC Chambe

r

TC Chambe

r

TC Chambe

r TC Chambe

r

TC Chambe

r TC Chambe

r

53

1.29 m

1 m

51

^sink

0.9m

GM Milling Room/

Temporary Storage

Ref Ref Ref

1.395 m

shaker

LEGEND:

- Electrical outlet (2 gang) - Water outlet and drain

- no backrest chair/stool - 2-way swing door

Other Notes:

• Wall – 0.13m thick (assuming)

• Electric outlets about 1m apart

(15)

Second Floor – GTL Isolation and Gel Doc Room

GTL Isolation Room

58

GTL Gel Doc Room , PCR Prep, and

Electrophoresis

57 56

sink

Clean bench

54

Clean benchBiosafety cabinet

ref

cart Biosafety

cabinet

5.05 M

6.0 M

5.76 M

LEGEND:

- Electrical outlet (2 gang) - Water outlet and drain

- with backrest chair/stool

Other Notes:

• Wall – 0.13m thick (assuming)

• Electric outlets about 1m apart

(16)

65

61

Furnac e

3.5 M 4.19 M

67 64

Refrid gerat or

Second Floor – Common Equipment Room and Sequencing Room

DNA Library Prep Sequencing Area

66 4.9 M 4.9 M

Refrid gerat or

LEGEND:

- Electrical outlet (2 gang) - sliding door

Other Notes:

• Wall – 0.13m thick (assuming)

(17)

Second Floor – TCL Dark Room and Light Room

Bench (Unlighted) Bench (Unlighted) Bench (Unlighted) Bench (Unlighted) Bench (Unlighted)

Dehum .

Dehum.

Bench (Lighted) Bench (Lighted)

Bench (Lighted) Bench (Lighted) Bench (Lighted)

Ladder

LEGEND:

- Electrical outlet (2 gang) - Water outlet and drain - sliding door

Other Notes:

• Wall – 0.13m thick (assuming)

(18)

Second Floor – TCL

Auto. Auto.

77

Rack

Sink Sink Sink

Clean Bench with Glass Bead Sterilizer

Bioref

73

74

Auto.

Clean Bench with Glass Bead Sterilizer Clean Bench with Glass Bead SterilizerClean Bench with Glass Bead Sterilizer

Rack

79

Water purifica tion

5.68 m

4.15 m

5.68 m

4.25 m

LEGEND:

- no backrest chair/stool - sliding door

Other Notes:

Sink

Rack / Cabinet

(19)

Laboratory Storage 2F

4.0 m

4.4 m