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Determination of Duck Egg fertility using Machine Vision - UPLB

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JEROME MONDALA JOPIA

SUBMITTED TO THE FACULTY OF AGRICULTURAL MACHINERY DIVISION INSTITUTE OF AGRICULTURAL ENGINEERING

COLLEGE OF ENGINEERING AND AGRO-INDUSTRIAL TECHNOLOGY UNIVERSITY OF THE PHILIPPINES LOS BAÑOS

IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE

DEGREE OF

BACHELOR OF SCIENCE IN AGRICULTURAL ENGINEERING Major in Agricultural Power and Machinery Engineering

APRIL 2010

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TABLE OF CONTENTS

PAGE

TITLE PAGE i

APPROVAL PAGE ii

BIOGRAPHICAL SKETCH iii

ACKNOWLEDGEMENT iv

TABLE OF CONTENTS vi

LIST OF TABLES ix

LIST OF FIGURES x

LIST OF APPENDICES xii

ABSTRACT xiii

INTRODUCTION 1

Significance of the Study 2

Objectives of the Study 3

Scope and Limitation of the Study 3

Time and Place of Study 4

REVIEW OF LITERATURE 5

Duck Industry 5

Duck Egg 5

Balut and Penoy 6

Egg Candling 7

Human Vision 10

Machine Vision 11

Cameras and Optics 12

Lighting 13

Frame Grabber 13

Computer 14

Software 14

Image Processing 15

Image Acquisition 15

Image Pre-processing 15

Segmentation 16

Object Interpretation 16

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PAGE

Classification 16

Accuracy and Precision 17

MATERIALS AND METHODS 19

Materials 19

Camera 19

Egg Candling Chamber 19

Video Capture Card 20

Computer 21

Software 21

Methods 22

Design of Candling System 22

Development of Software 23

Identification of Parameters 23

Software Development 23

Experimental Procedure 23

Test for Precision 23

Test for Accuracy 24

RESULTS AND DISCUSSION 25

Development of Software 25

Identification of Parameters 25

Designing the Graphical User Interface (GUI) 26

Process Flow 29

Image Acquisition 30

Image Pre-processing 31

Image Segmentation 32

Object Interpretation 33

Classification 34

Test for Precision 35

Test for Precision in Measuring the Egg Size 35 Coefficient of Variance in Measuring the Egg Size 37 Analysis of Variance in Measuring the Egg Size 39

Test for Precision in Measuring Embryo Size 40

Test for Accuracy 42

Test for Accuracy in Classifying the Fertility of Duck Eggs Based from the Expert

42

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PAGE Test for Accuracy in Classifying the Fertility of Duck Eggs Based

on Actual Classification

45

SUMMARY AND CONCLUSION 47

RECOMMENDATIONS 49

LIST OF REFERENCES 50

APPENDICES 53

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LIST OF TABLES

TABLE PAGE

1 Number of egg pixels at 24 orientation angles 38 2 Summary of the ANOVA for the variability of egg sized among egg

samples and on the different orientation angles

39

3 Software’s classification and expert’s classification of a 10-day old duck egg

43

4 Software’s classification and expert’s classification of a 17-day old duck egg

44

4 Comparison of the software's classification and expert's classification from the actual classification

46

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LIST OF FIGURES

FIGURE PAGE

1 Parts of an egg 6

2 Manual egg candling 7

3 Interpretation of candled chicken images 9

4 Parts of the human eye with an enlargement of the retina 10

5 Accuracy and precision target analogy 17

6 SONY DCR-TRV460 digital video camera recorder 19

7 Egg candling chamber 20

8 Video capture card 20

9 Computer 21

10 Candling system 22

11 Images of candled duck eggs at different stages 26

12 Main form 28

13 Background settings form 28

14 Flow chart of image processing 29

15 Acquired image of duck eggs at different stages 30 16 Pre-processed image of duck eggs at different stages 31 17 Segmented image of duck eggs at different stages 32 18 Interpreted image of duck eggs at different stages 34 19 Maximum, minimum, and average egg pixels of ten egg samples 36 20 Egg pixel of egg samples at 24 orientation angles 36 21 Maximum, minimum, and average embryo percentage of ten egg

samples

40

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PAGE 22 Embryo percentage of egg samples at 24 orientation angles 41

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LIST OF APPENDICES

APPENDIX PAGE

A Images of a duck egg at 24 orientation angles 54

B Measurements of conditional parameter 56

C Analysis of Variance (ANOVA) 58

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ABSTRACT

JOPIA, JEROME MONDALA. University of the Philippines Los Baños, April 2010.

Determination of Duck Egg Fertility Using Machine Vision.

Adviser: Marvin C. Petingco

A machine vision system, which consists of a Sony DCR - TRV 460 Digital Video Cam Recorder, an egg candling chamber, a TV capture card, a Pentium 4 personal computer at 2.66 Ghz speed, and software (EC 1.0) for determining duck egg fertility was developed and tested.

Ten samples of 10-day old eggs were used to test the precision of the software in measuring the size of the egg at 24 orientation angles. On the other hand, 30, 10-day old egg samples (15 fertile and 15 infertile) and 30, 17-day old egg samples (15 fertile and 15 infertile) were used to test precision of the software in classifying the fertility of duck eggs. For the accuracy of the software in classifying the duck egg fertility, 30, 17-day old duck eggs were used.

The results showed that the developed software has a 99.4 percent precision in measuring the size of the egg. The percent accuracies, using the expert’s classification as standard, were 93.33% and 96.67% for the 10-day old and 17-day old duck egg, respectively. The results also showed a hundred percent accuracy in classifying the fertility of a 17-day old duck egg.

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