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Genetic diversity, population structure and demographic history of the tropical eel Anguilla bicolor pacifica in Southeast Asia using mitochondrial DNA control region sequences

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Genetic diversity, population structure and demographic history of the tropical eel Anguilla bicolor pacifica in Southeast Asia using mitochondrial DNA control region sequences

Melfa Marini1,2*, Ivane R. Pedrosa-Gerasmio3 Mudjekeewis D Santos4, Takuro Shibuno1, Ayu Daryani1, Maria Rowena R. Romana-Eguia5, and Arif Wibowo1,2

1Inland Fishery Resources Development and Management Department, Southeast Asian Fisheries Development Center, Jl. Gubernur H.A Bastari No 08, Jakabaring Palembang 30252, Indonesia

2Research Institute for Inland Fishery, Ministry of Marine Affairs and Fisheries, Jl. Gubernur H. A. Bastari No. 08, Kel. Silaberanti, Kec. Seberang Ulu I, Palembang, South Sumatera, 30252, Indonesia

3Department of Marine Sciences, College of Science and Mathematics, Mindanao State University – Iligan Institute of Technology, Iligan City, Philippines

4Genetic Fingerprinting Laboratory, National Fisheries Research and Development Institute, 101 Mother Ignacia St., Quezon City, Philippines

5Aquaculture Department, Southeast Asian Fisheries Development Center, Binangonan Freshwater Station, Binangonan, 1940, Rizal, Philippines

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Supplementary File

Supplementary Figure 1. Median-joining haplotype network for 151 samples of A. bicolor pacifica from three sampling sites: Vietnam (yellow), Philippines (blue) and Indonesia (black) drawn using NETWORK v4.6.1.0 (copyright 2004-2012, Fluxus Technologies Ltd.; http://www.fluxus-engineering.com). Node sizes are proportional to haplotype frequencies; in red hypothetical intermediate haplotypes are indicated.

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Supplementary Figure 2. Nucleotide sequences of the mtDNA control region haplotypes (n=47) of A. bicolor pacifica from Phu Yen, Vietnam. The number of specimens per haplotype are shown in parenthesis. Percent identity (compared to the first haplotype sequence) is provided at the end of the alignment.

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Supplementary Figure 3. Nucleotide sequences of the mtDNA control region haplotypes (n=52) of A. bicolor pacifica from General Santos, Philippines.

The number of specimens per haplotype are shown in parenthesis. Percent identity (compared to the first haplotype sequence) is provided at the end of the alignment.

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Supplementary Figure 4. Nucleotide sequences of the mtDNA control region haplotypes (n=39) of A. bicolor pacifica from Palu, Indonesia. The number of specimens per haplotype are shown in parenthesis. Percent identity (compared to the first haplotype sequence) is provided at the end of the alignment.

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*Correspondence Department of Biological Sciences and Environmental Studies, College of Science and Mathematics, University of the Philippines Mindanao, Mintal, Tugbok District,

*Correspondence Department of Biological Sciences and Environmental Studies, College of Science and Mathematics, University of the Philippines Mindanao, Mintal, Tugbok District,