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DNA Sequencing Core Facility - Philippine Genome Center

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Funded by the Department of Science and Technology (DOST) - Philippine Council for Industry, Energy, and Emerging Technology Research and Development (PCIEERD), DNA Sequencing Core Facility (DSCF) was integrated to the day-to-day operations of the University’s Philippine Genome Center by late 2013. Both the DNA Sequencing Core Facility and the Core Facility for Bioinformatics began offering laboratory atnd technical services pertinent to genomics and bioinformatics research.

Identifying appropriate sequencing platforms that will efficiently meet your research goals

ADDRESS

LANDLINE EMAIL WEBSITE

Philippine Genome Center Building A. Ma. Regidor Street

University of the Philippines Diliman Quezon City 1101, Philippines +63 • 2 • 8981 • 8500 local 4707 [email protected] [email protected]

https://pgc.up.edu.ph

Contact Us

[ SCAN CODE ]

PHILIPPINE GENOME CENTER UNIVERSITY OF THE PHILIPPINES

DNA Sequencing Core Facility

DS CF DS

CF

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ABOUT THE FACILITY LOCATION

SERVICES OFFERED

Identifying appropriate sequencing strategies to efficiently meet research goals. DSCF offers a full range of DNA sequencing services from single gene sequencing of capillary sequencers to medium and high-throughput sequencing of Illumina, Ion Torrent and PacBio platforms. It is a central facility that accepts sequencing service requests from clients, conducts quality control tests, analysis and characterization to samples, and provides consultation on the selection of technology platforms in the most cost-effective, reliable and efficient way possible.

DNA Sequencing Core Facility

Nucleic Acid Extraction Nucleic Acid Quantification Polymerase Chain Reaction (PCR) PCR Purification

Sanger Sequencing Library Preparation

Next Generation Sequencing Equipment Use

Trainings (available upon request) +

+ + + + + + + +

APPLICATIONS

Whole Genome Sequencing Whole Exome Sequencing

Transcriptome Sequencing (RNA-Seq) 16S Metagenome Sequencing Amplicon / Targeted Sequencing Genotyping

+ + + + + +

TECHNOLOGIES

Ion Torrent PGM (100MB- 1GB) Ion Torrent Proton (10GB) MiSeq RUO (15GB) MiSeq FGx (15 GB) NovaSeq 6000 (3000 GB) NextSeq 500 (120 GB) PacBio Sequel (20GB)

Affymetrix GeneTitan Microarray +

+ + + + + + + UGF

A T G C T G A T G C T G A C T C C A A C T C C A C A T C G A T G C A T C G A T G G A G G A A T G A G G A A T G T A A G A A G T A A G A A T C G A T G C T C G A T G C G G A A T G C T G G A A T G C T A A T G G T A A A T G G T A G A A T C G G A A T C G

WORKFLOW WORKFLOW

Sample

Preparation Library Preparation

Sequencing Initial Data Processing

Data Analysis

Referensi

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