View of การตรวจวัดปรอท(II)ด้วยเอโซเมทีนเอชโดยอาศัยเทคนิคสเปกโทรโฟโตเมทรี A Spectrophotometric Method for Mercury(II) Detection with Azomethine H

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°“√μ√«®«—¥ª√Õ∑ (II) ¥â«¬‡Õ‚´‡¡∑’π‡Õ™‚¥¬Õ“»—¬‡∑§π‘§

‡ª°‚∑√‚ø‚μ‡¡∑√’

ß“¡®‘μ ‰æ√ß“¡

^1*

·≈– »ÿ¿°“≠®πå √—μπ°√

¹

∫∑§—¥¬àÕ

ß“π«‘®—¬π’È‡ªìπ°“√»÷°…“°“√«‘‡§√“–Àå Hg²⁺ ¥â«¬‡Õ‚´‡¡∑’π‡Õ™ (L) ‚¥¬Õ“»—¬‡∑§π‘§ ‡ª°‚∑√

‚ø‚μ‡¡∑√’ Hg²⁺ ∑”ªØ‘°‘√‘¬“°—∫‡Õ‚´‡¡∑’π‡Õ™‡°‘¥ “√ª√–°Õ∫‡™‘ß´âÕπ ’‡À≈◊Õß„π “√≈–≈“¬Õ–´‘‡μ∑∫—ø‡øÕ√å pH 4 ®“°°“√»÷°…“¥â«¬«‘∏’¢Õß Job æ∫«à“ Hg²⁺ ∑”ªØ‘°‘√‘¬“°—∫ L „πÕ—μ√“ à«π 1:1 ‡°‘¥‡ªìπ “√ª√–°Õ∫

‡™‘ß´âÕπ„π√Ÿª HgL §à“°“√¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm · ¥ß§«“¡ —¡æ—π∏å·∫∫‡ âπμ√ß°—∫§«“¡‡¢â¡¢âπ¢Õß Hg²⁺ „π™à«ß 3.0Ó10^-6-1.0Ó10^-3 M (R²=0.9992) §à“¢’¥®”°—¥μË” ÿ¥„π°“√«‘‡§√“–Àå·≈–¢’¥®”°—¥

μË” ÿ¥„π°“√«‘‡§√“–Àå‡™‘ßª√‘¡“≥¡’§à“‡∑à“°—∫ 3.0Ó10^-6 M ·≈– 5.0Ó10^-6 M μ“¡≈”¥—∫ §«“¡‡∑’Ë¬ß

„π°“√«‘‡§√“–Àå‡¡◊ËÕ«—¥§à“°“√¥Ÿ¥°≈◊π· ß¢Õß “√≈–≈“¬ Hg²⁺5.0Ó10^-5 M ·≈– 1.0Ó10^-4 M Hg²⁺

´È” 5 §√—Èß¡’§à“‡∑à“°—∫ 2.84% ·≈– 1.22% μ“¡≈”¥—∫ ‡∑§π‘§«‘‡§√“–Àåπ’È “¡“√∂π”‰ªª√–¬ÿ°μå„™â„π°“√

«‘‡§√“–Àå Hg²⁺ „ππÈ”μ—«Õ¬à“ß‰¥â

§” ”§—≠: ª√Õ∑ ‡Õ‚´‡¡∑’π‡Õ™ ‡ª°‚∑√‚ø‚μ‡¡∑√’

1¿“§«‘™“‡§¡’ §≥–«‘∑¬“»“ μ√å ¡À“«‘∑¬“≈—¬»√’π§√‘π∑√«‘‚√≤

*ºŸâπ‘æπ∏åª√– “πß“π, e-mail: [email protected]

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A Spectrophotometric Method for Mercury (II) Detection with Azomethine H

Ngamjit Praingam

^1*

and Supakan Rattanakon

¹

ABSTRACT

A spectrophotometric determination of Hg²⁺ with azomethine H (L) was studied.

Hg²⁺ reacts with azomethine H to form a yellow complex in an acetate buffer solution of pH 4.

Jobûs method of continuous variation suggested 1:1 metals to ligand stoichiometry for Hg²⁺ complex (HgL). The absorption measurements at 425.5 nm were linearity related to the concentration of Hg²⁺ in the range of 3.0Ó10^-6-1.0Ó10^-3 M (R²=0.9992). The limit of detection and limit of quantification were found to be 3.0Ó10^-6 M and 5.0Ó10^-6 M, respectively. Method precision (repeatability) were determined by relative standard deviation for five repeated measurements at 5.0Ó10^-5 M and 1.0Ó10^-4 M Hg²⁺ were 2.84% and 1.22%, respectively.

The proposed method was applied successfully for the determination of Hg²⁺ content in water samples.

Keywords: Mercury, Azomethine H, Spectrophotometry

1Department of Chemistry, Faculty of Science, Srinakharinwirot University.

*Corresponding author, e-mail: [email protected]

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∫∑π”

°“√«‘‡§√“–ÀåÀ“ª√‘¡“≥‰ÕÕÕπ¢Õß‚≈À–Àπ—°∑’Ëªπ‡ªóôÕπ„π ‘Ëß·«¥≈âÕ¡ ¢∫«π°“√º≈‘μ∑“ß Õÿμ “À°√√¡ ∑“ß™’««‘∑¬“·≈–∑“ß°“√·æ∑¬å‡ªìπ ‘Ëß∑’Ëπà“ π„®‡ªìπÕ¬à“ß¬‘Ëß ‡π◊ËÕß®“°¡’§«“¡ ”§—≠μàÕ

°“√¥”√ß™’«‘μ·≈– ÿ¢¿“æ¢Õß¡πÿ…¬å °“√ªπ‡ªóôÕπ¢Õßª√Õ∑„π ‘Ëß·«¥≈âÕ¡¡—°Õ¬Ÿà„π√Ÿª Hg²⁺ ·≈– Hg₂²⁺

´÷Ëß “¡“√∂π”‰ª Ÿà‚√§¡‘π“¡“μ–‡¡◊ËÕ – ¡„π√à“ß°“¬ Õ’°∑—Èß¬—ß “¡“√∂‡ª≈’Ë¬π‡ªìπª√Õ∑Õ‘π∑√’¬å´÷Ëß¡’æ‘…√â“¬

·√ß‚¥¬Õ“»—¬·∫§∑’‡√’¬ ·≈–‡¢â“ ŸàÀà«ß‚´àÕ“À“√„π√–∫∫π‘‡«»‰¥â μ—«Õ¬à“ßª√Õ∑Õ‘π∑√’¬å∑’Ë¡’æ‘…√â“¬·√ß ‡™àπ

‡¡∑‘≈‡¡Õ√å§‘«√’ “¡“√∂∑”≈“¬√–∫∫ª√– “∑ °≈â“¡‡π◊ÈÕ·≈–∑”„Àâ∂÷ß·°à™’«‘μ‰¥â [1] ª√‘¡“≥ª√Õ∑∑’ËÕπÿ≠“μ

„Àâ¡’‰¥â„ππÈ”¥◊Ë¡·≈–Õ“À“√∑–‡≈¡’§à“‡∑à“°—∫ 1 _μg/L ´÷Ëß°”Àπ¥‚¥¬Õß§å°“√Õπ“¡—¬‚≈° (World Health Organization, WHO) ·≈– 0.5 mg/kg ´÷Ëß°”Àπ¥‚¥¬°√–∑√«ß “∏“√≥ ÿ¢ [2, 3]

ªí®®ÿ∫—π‡∑§π‘§∑’Ë„™â°“√«‘‡§√“–Àåª√Õ∑¡’À≈“¬‡∑§π‘§‡™àπ ‚æ‡∑π™‘‚Õ‡¡∑√’ [4] Õ‘π¥—°∑‘ø≈’

§—æ‡æ‘≈æ≈“ ¡“·¡ ‡ª°‚∑√‚ø‚μ‡¡∑√’ [5] ‡Õ°´‡√¬åø≈ŸÕÕ‡√ ‡´πμå ‡ª°‚∑√‚ø‚μ‡¡∑√’ [6] Õ–μÕ¡¡‘§

·Õæ´Õ√åæ™—π ‡ª°‚∑√‚ø‚μ‡¡∑√’ [7] ‚§≈¥å‡«‡æÕ√åÕ–μÕ¡‘°·Õæ´Õ√åæ™—π ‡ª°‚∑√‚ø‚μ‡¡∑√’ [8]

ø≈ŸÕÕ‡√ ‡´πμå ‡ª°‚∑√‚ø‚μ‡¡∑√’ [9, 10] ∂÷ß·¡â«à“‡∑§π‘§‡À≈à“π’È®–¡’§«“¡‰«·≈–§«“¡‡∑’Ë¬ß Ÿß·μà°Á¡’

¢—ÈπμÕπ„π°“√‡μ√’¬¡μ—«Õ¬à“ß ‡§√◊ËÕß¡◊Õ¡’√“§“ Ÿß·≈–´—∫´âÕπ´÷ËßμâÕßÕ“»—¬ºŸâªØ‘∫—μ‘°“√∑’Ë¡’§«“¡√Ÿâ·≈–∑—°…–

°“√∑”ß“π¢Õß‡§√◊ËÕß ¥—ßπ—Èπ‡æ◊ËÕ≈¥§«“¡¬ÿàß¬“°·≈–§à“„™â®à“¬„π°“√«‘‡§√“–Àåª√Õ∑‡∑§π‘§ ‡ª°‚∑√‚ø‚μ

‡¡∑√’·≈–μ—«μ√«®«—¥∑“ß· ß (optical sensor) ®÷ß‡ªìπ∑’Ëπ‘¬¡¡“° πÕ°®“°®– “¡“√∂ —ß‡°μ°“√

‡ª≈’Ë¬π·ª≈ß ’‰¥â¥â«¬μ“‡ª≈à“·≈â«¬—ß “¡“√∂«‘‡§√“–Àåª√‘¡“≥‰¥âÕ¬à“ß¡’ª√– ‘∑∏‘¿“æ‡¡◊ËÕ‡≈◊Õ°„™â√’‡Õ‡®πμå À√◊Õμ—«μ√«®«—¥∑’Ë‡À¡“– ¡ μ—«μ√«®«—¥∑’Ë„™â„π°“√«‘‡§√“–Àå Hg²⁺ ¡—°¡’Õ–μÕ¡∑’Ë„Àâ§ŸàÕ‘‡≈Á°μ√Õπ§◊Õ ‰π‚μ√‡®π ÕÕ°´‘‡®π·≈–´—≈‡øÕ√å ´÷Ëß “¡“√∂‡°‘¥æ—π∏–‚§ÕÕ√å¥‘‡π∑‚§‡«‡≈πμå°—∫ Hg²⁺ ‰¥â‡™àπ Terphenyl derivative [9] Thiooxorhodamine [10] Phenothiazine [11] 2,3-diferrocenylquinoxaline [12] 4-(2-pyridylazo)- resorcinol [13] Benzothiazolium Hemicyanine [14] ≈‘·°π¥å‡Õ‚´‡¡∑’π‡Õ™‡ªìπ≈‘·°π¥å∑’Ë¡’À¡Ÿà„Àâ Õ‘‡≈Á°μ√Õπ§◊ÕÀ¡Ÿà‰Œ¥√Õ°´‘≈·≈–À¡ŸàÕ‘¡¡’π π‘¬¡„™â‡ªìπμ—«μ√«®«—¥‚∫√Õπ„πμ—«Õ¬à“ß Õßÿàπ [15] ¥‘π [16]

πÈ” [17] ‚¥¬‡°‘¥ “√ª√–°Õ∫‡™‘ß´âÕπ ’‡À≈◊Õß°—∫‚∫√Õπ ®“°°“√»÷°…“æ∫«à“°“√„™â‡Õ‚´‡¡∑’π‡Õ™‡ªìπμ—«

μ√«®«—¥¡’πâÕ¬¡“°·≈–‚¥¬∑—Ë«‰ª·≈â«μ—«Õ¬à“ß∑’Ë„™â„π°“√«‘‡§√“–Àåª√Õ∑¡—°‰¡à¡’‚∫√ÕπªπÕ¬Ÿà¥â«¬ „πß“π

«‘®—¬π’È®–‡ªìπ°“√»÷°…“°“√«‘‡§√“–Àå Hg²⁺ ¥â«¬≈‘·°π¥å‡Õ‚´‡¡∑’π‡Õ™‚¡‚π‚´‡¥’¬¡´÷Ëß≈–≈“¬πÈ”‰¥â¥’·≈–

∑”°“√μ√«®«—¥ª√‘¡“≥ Hg²⁺ ‚¥¬Õ“»—¬‡∑§π‘§ ‡ª°‚∑√‚ø‚μ‡¡∑√’ ¡’°“√»÷°…“ ¿“«–∑’Ë‡À¡“– ¡·≈–ªí®®—¬

μà“ßÊ ∑’Ë¡’º≈μàÕ°“√«‘‡§√“–Àå ‡™àπ pH μ—«√∫°«π Õ—μ√“ à«π‚¡≈√–À«à“ß Hg²⁺ ·≈–‡Õ‚´‡¡∑’π‡Õ™„π “√

‡™‘ß´âÕπ

Õÿª°√≥å·≈–«‘∏’∑¥≈Õß

“√‡§¡’∑’Ë„™â‰¥â·°à Azomethine H monosodium salt hydrate (L), (NH₄)₂FeSO₄.6H₂O, MnSO₄.H₂O, AgNO₃, NiSO₄.6H₂O, Co(NO₃).6H₂O, CuSO₄, Hg(CH₃COO)₂ ·≈– Cd(NO₃)₂, Pb(NO₃)₂ ‡ªìπ “√ analytical grade ·≈–‡μ√’¬¡‡ªìπ “√≈–≈“¬¥â«¬πÈ”°≈—Ëπμ“¡§«“¡‡¢â¡¢âπ∑’Ë μâÕß°“√ “√≈–≈“¬‡μ√’¬¡„À¡à‡¡◊ËÕ„™â«—¥°“√¥Ÿ¥°≈◊π· ß¥â«¬ Shimadzu double beam UV-VIS spectrophotometer Model UV-2101 PC ·≈–«—¥ pH ¥â«¬ Metrohm pH meter

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°“√‡μ√’¬¡ “√≈–≈“¬‡æ◊ËÕπ”‰ª«—¥°“√¥Ÿ¥°≈◊π· ß∑”‚¥¬º ¡ “√≈–≈“¬ Hg²⁺ ·≈–

“√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™∑’Ë§«“¡‡¢â¡¢âπ·≈–ª√‘¡“μ√∑’ËμâÕß°“√‡μ√’¬¡·≈â«ª√—∫ “√≈–≈“¬„Àâ¡’ pH 4 ¥â«¬

Õ–´‘‡μ∑∫—ø‡øÕ√å ®“°π—Èπª√—∫ª√‘¡“μ√¥â«¬πÈ”°≈—Ëπ π”‰ª«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë§«“¡¬“«§≈’Ëπ 425.5 nm

„π°“√»÷°…“º≈¢Õß pH μàÕ°“√«‘‡§√“–Àå®–¡’°“√ª√—∫ pH 2-12 ¥â«¬ NaOH ‡¢â¡¢âπ 1 M ·≈– HNO₃

‡¢â¡¢âπ 1 M ∑”°“√‰∑‡∑√μ “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™¥â«¬ “√≈–≈“¬ Hg²⁺ ·≈â«π”‰ª«—¥ ‡ª°μ√“∑’Ë 300-600 nm ·≈–„π°“√»÷°…“§«“¡‡©æ“–‡®“–®ß„π°“√μ√«®«—¥ª√Õ∑ ®–¡’°“√»÷°…“μ—«√∫°«π°“√

«‘‡§√“–Àå‚¥¬°“√‡μ‘¡ “√≈–≈“¬ Fe²⁺, Mn²⁺, Ag⁺, Ni²⁺, Co²⁺, ·≈– Cu²⁺ ·μà≈–™π‘¥∑’Ë§«“¡‡¢â¡¢âπ 5 ‡∑à“¢Õß§«“¡‡¢â¡¢âπ Hg²⁺

º≈°“√∑¥≈Õß

“√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™‡¢â¡¢âπ 5 Ó 10^-4 M ‰¡à¡’ ’ ‡¡◊ËÕπ”¡“º ¡°—∫ “√≈–≈“¬ Fe²⁺, Mn²⁺, Ag⁺, Ni²⁺, Co²⁺, ·≈– Cu²⁺ ∑’Ë¡’§«“¡‡¢â¡¢âπ‡∑à“°—π ∑’≈–™π‘¥æ∫«à“‰¡à¡’°“√‡ª≈’Ë¬π·ª≈ß ’

¬°‡«âπ Hg²⁺ ´÷Ëß “√≈–≈“¬®–‡ª≈’Ë¬π‡ªìπ ’‡À≈◊Õß∑—π∑’ ‡ª°μ√“°“√¥Ÿ¥°≈◊π· ß∑’Ë 300-600 nm

· ¥ß¥—ß √Ÿª∑’Ë 1 æ∫«à“ “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™¥Ÿ¥°≈◊π· ß‰¥â¥’∑’Ë _λ_max=340 nm (_ε=11,280 M^-1 cm^-1) ¥—ß√Ÿª 1a ·≈– “√≈–≈“¬ ’‡À≈◊Õß¢Õß “√ª√–°Õ∫‡™‘ß´âÕπª√Õ∑¥Ÿ¥°≈◊π· ß‰¥â¥’∑’Ë _λ_max= 425.5 nm (_ε=1,740 M^-1 cm^-1) ·≈–°“√¥Ÿ¥°≈◊π· ß∑’Ë _λ_max=340 nm ®–≈¥≈ß¥—ß√Ÿª∑’Ë 1b

√Ÿª∑’Ë 1 ‡ª°μ√“¢Õß (a) “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™‡¢â¡¢âπ 5.0Ó10^-5 M ·≈– (b) “√≈–≈“¬º ¡∑’Ë¡’

§«“¡‡¢â¡¢âπ Hg²⁺ ·≈–‡Õ‚´‡¡∑’π‡Õ™‡∑à“°—∫ 5Ó10^-5 M

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°“√»÷°…“º≈¢Õß pH ∑’Ë¡’μàÕ°“√μ√«®«—¥ª√Õ∑

‡¡◊ËÕπ” “√≈–≈“¬º ¡√–À«à“ß “√≈–≈“¬ Hg²⁺ ·≈– “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™∑’Ë¡’§«“¡‡¢â¡¢âπ

‡∑à“°—π∑’Ë 4.0Ó10^-5 M ·≈â«ª√—∫„Àâ¡’ pH 2.2-11.8 π”¡“«—¥°“√¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm ∑”´È” pH

≈– 3 §√—Èß §à“∑’Ë‰¥âπ”¡“ √â“ß°√“ø· ¥ß¥—ß√Ÿª∑’Ë 2 æ∫«à“ pH ¡’º≈μàÕ°“√μ√«®«—¥ª√Õ∑ ‚¥¬∑’Ë pH ‡∑à“°—∫

4 ‡ªìπ§à“ pH ¢Õß “√≈–≈“¬º ¡∑’Ë„Àâ§à“°“√¥Ÿ¥°≈◊π· ß Ÿß ÿ¥ ·≈–‡¡◊ËÕ “√≈–≈“¬‡ªìπ°√¥À√◊Õ‡∫ ¡“°

¢÷Èπ®–„Àâ§à“°“√¥Ÿ¥°≈◊π· ß≈¥≈ß·≈– “√≈–≈“¬¬—ß§ß‡ªìπ ’‡À≈◊Õß ‡¡◊ËÕ∑”°“√∑¥ Õ∫º≈¢Õß pH μàÕ “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™æ∫«à“ “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™‰¡à¡’°“√‡ª≈’Ë¬π·ª≈ß ’‡¡◊ËÕ pH ‡ª≈’Ë¬π ¥—ßπ—Èπ º≈¢Õß pH ∑’Ë∑”„Àâ°“√¥Ÿ¥°≈◊π· ß≈¥≈ßπ—Èπ¡“®“°°“√μ°μ–°Õπ‰Œ¥√Õ°‰´¥å¢Õßª√Õ∑„π “√≈–≈“¬‡∫

À√◊Õ°“√·μ°μ—«¢Õß “√ª√–°Õ∫‡™‘ß´âÕπ¢Õßª√Õ∑„π “√≈–≈“¬°√¥‡π◊ËÕß®“°¡’ H⁺ ¡“·¬àß®—∫°—∫≈‘·°π¥å

‡Õ‚´‡¡∑’π‡Õ™¥—ßπ—Èπ ¿“«–∑’Ë‡À¡“– ¡„π°“√«‘‡§√“–Àåª√Õ∑¥â«¬‡∑§π‘§π’È §◊Õ pH 4 „π°“√∑¥≈Õß®÷ßª√—∫

pH ¢Õß “√≈–≈“¬¥â«¬Õ–´‘‡μ∑∫—ø‡øÕ√å pH 4 °àÕπ∑”°“√«‘‡§√“–Àå

√Ÿª∑’Ë 2 °√“ø√–À«à“ß§à“ pH ·≈–°“√¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm ¢Õß “√≈–≈“¬º ¡√–À«à“ß “√≈–≈“¬

Hg²⁺ ·≈– “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™∑’Ë¡’§«“¡‡¢â¡¢âπ 4.0Ó10^-5 M

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°“√»÷°…“ Stoichiometric ratio ¢Õß “√ª√–°Õ∫‡™‘ß´âÕπ¢Õßª√Õ∑

‡¡◊ËÕπ”§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm ¢Õß “√≈–≈“¬º ¡√–À«à“ß “√≈–≈“¬ Hg²⁺ ·≈–

“√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™ (L) ‡¢â¡¢âπ 2.0Ó10^-3 M „πª√‘¡“μ√μà“ßÊ °—π∑’Ë pH 4 ¡“ √â“ß Jobûs plot

¥—ß√Ÿª∑’Ë 3 æ∫«à“ ‡¡◊ËÕ √â“ß‡ âπ·π«‚πâ¡‡ âπμ√ß 2 ‡ âπ®–μ—¥°—π∑’ËÕ—μ√“ à«π‚¡≈‡∑à“°—∫ 0.5 ´÷ËßÀ¡“¬§«“¡«à“

“√ª√–°Õ∫‡™‘ß´âÕπ¢Õßª√Õ∑‡°‘¥®“°ªØ‘°‘√‘¬“√–À«à“ß Hg²⁺ : ‡Õ‚´‡¡∑’π‡Õ™„πÕ—μ√“ à«π‚¡≈ 1 : 1

· ¥ß∂÷ß Ÿμ√‡§¡’¢Õß “√ª√–°Õ∫‡™‘ß´âÕπª√Õ∑„π√Ÿª¢Õß HgL ·≈–‡ πÕ‚§√ß √â“ß∑’ËÕ“®‡ªìπ‰ª‰¥â¢Õß “√ª√–°Õ∫‡™‘ß´âÕπ HgL ¥—ß√Ÿª∑’Ë 4 ´÷Ëß¡’‚§√ß √â“ß§≈â“¬°—∫ “√‡™‘ß´âÕπ√–À«à“ß‚∫√Õπ·≈–‡Õ‚´

‡¡∑’π‡Õ™∑’Ë¡’Õ—μ√“ à«π‚¡≈·∫∫ 1 : 1 ‡™àπ°—π [18] ‚¥¬∑’ËÀ¡Ÿà„Àâ§ŸàÕ‘‡≈Á°μ√Õπ∑—Èß “¡¢Õß‡Õ‚´‡¡∑’π‡Õ™

§◊Õ À¡ŸàÕ–¡‘‚π¢Õß‡´§—π¥“√’Õ‘¡’π À¡Ÿà‰Œ¥√Õ°´‘≈¢ÕßøïπÕ≈·≈–À¡Ÿà‰Œ¥√Õ°´‘≈¢Õß·πø∑Õ≈ √â“ßæ—π∏–

‚§ÕÕ√å¥‘‡π∑‚§‡«‡≈πμå°—∫ Hg²⁺ ‡°‘¥‡ªìπ«ßÀ°‡À≈’Ë¬¡ Õß«ßμ‘¥°—π„π “√ª√–°Õ∫‡™‘ß´âÕπ HgL

√Ÿª∑’Ë 3 Jobûs plot ¢Õß “√≈–≈“¬º ¡√–À«à“ß “√≈–≈“¬ Hg²⁺ ·≈– “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™ (L) ∑’Ë pH 4 «—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm

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√Ÿª∑’Ë 4 ‚§√ß √â“ß∑’Ë‡ªìπ‰ª‰¥â¢Õß “√ª√–°Õ∫‡™‘ß´âÕπ HgL

°“√‰∑‡∑√μ¢Õß “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™¥â«¬ “√≈–≈“¬ Hg²⁺

‡¡◊ËÕ‰∑‡∑√μ “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™‡¢â¡¢âπ 1 Ó 10^-4 M ¥â«¬ “√≈–≈“¬ Hg²⁺ ‡¢â¡¢âπ 0.0 M-1.5Ó10^-4 M ·≈â«π”‰ª«—¥ ‡ª°μ√“„π™à«ß§«“¡¬“«§≈◊Ëπ 300-600 nm º≈· ¥ß¥—ß√Ÿª∑’Ë 5 ®“°

‡ª°μ√“· ¥ß§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë‡ª≈’Ë¬π·ª≈ß‰ª‡ªìπøíß°å™—π°—∫ª√‘¡“≥¢Õß Hg²⁺ ∑’Ë‡æ‘Ë¡¢÷Èπ ·∂∫°“√

¥Ÿ¥°≈◊π· ß¢Õß “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™∑’Ë§«“¡¬“«§≈◊Ëπ 340 nm ¡’§à“≈¥≈ß‡¡◊ËÕª√‘¡“≥¢Õß Hg²⁺

‡æ‘Ë¡¢÷Èπ ·≈–‡°‘¥ red-shift ‰¥â·∂∫°“√¥Ÿ¥°≈◊π· ß„À¡à∑’Ë§«“¡¬“«§≈◊Ëπ 425.5 nm ´÷Ëß¡’§à“°“√¥Ÿ¥°≈◊π· ß

‡æ‘Ë¡¢÷Èπμ“¡§«“¡‡¢â¡¢âπ¢Õß Hg²⁺ ∑’Ë‡æ‘Ë¡¢÷Èπ ´÷Ëß∑”„Àâ ’¢Õß “√≈–≈“¬‡ª≈’Ë¬π®“°‰¡à¡’ ’‡ªìπ ’‡À≈◊Õß‡¢â¡¢÷Èπ

·∂∫°“√¥Ÿ¥°≈◊π· ß„À¡àπ’È‡ªìπ¢Õß “√≈–≈“¬ ’‡À≈◊Õß¢Õß “√ª√–°Õ∫‡™‘ß´âÕπ (HgL) ·≈–‡¡’ËÕ‡μ‘¡ “√≈–≈“¬

Hg²⁺ ¡“°‡°‘πæÕ®–æ∫«à“§à“°“√¥Ÿ¥°≈◊π· ß‰¡à‡æ‘Ë¡¢÷Èπ ·≈–¡’ isosbestic point 1 ®ÿ¥∑’Ë§«“¡¬“«§≈◊Ëπª√–¡“≥

380 nm

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√Ÿª∑’Ë 5 ‡ª°μ√“°“√‰∑‡∑√μ¢Õß “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™‡¢â¡¢âπ 1Ó10^-4 M ¥â«¬ “√≈–≈“¬ Hg²⁺ ∑’Ë pH 4 ·≈–«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm

°“√μ√«®«—¥ª√Õ∑

‡¡◊ËÕπ” “√≈–≈“¬º ¡√–À«à“ß “√≈–≈“¬ Hg²⁺ ‡¢â¡¢âπ 3.0 Ó 10^-6-1.0 Ó 10^-3 M ·≈–

“√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™‡¢â¡¢âπ 2.0Ó 10^-3 M ∑’Ë pH 4 ¡“«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm ·≈–

√â“ß°√“ø¡“μ√∞“π¥—ß√Ÿª∑’Ë 6 ®“°º≈°“√∑¥≈Õßæ∫«à“§à“°“√¥Ÿ¥°≈◊π· ß‡æ‘Ë¡¢÷Èπ‡¡◊ËÕ§«“¡‡¢â¡¢âπ¢Õß “√

≈–≈“¬ Hg²⁺ ‡æ‘Ë¡¢÷Èπ ·≈–§«“¡ —¡æ—π∏å√–À«à“ß§«“¡‡¢â¡¢âπ¢Õß “√≈–≈“¬ Hg²⁺ 3.0Ó10^-6-1.0Ó10^-3 M °—∫§à“°“√¥Ÿ¥°≈◊π· ß‡ªìπ‡ âπμ√ß∑’Ë¥’¡’§à“ R² = 0.9992 §à“¢’¥®”°—¥μË” ÿ¥¢Õß°“√μ√«®«—¥ª√Õ∑

(Detection limit, LOD) ¡’§à“‡∑à“°—∫ 3.0 Ó10^-6 M (n =5) ´÷Ëß§”π«≥®“° (X_blank + 3SD_blank)/ slope (X_blank =§à“‡©≈’Ë¬°“√¥Ÿ¥°≈◊π· ß¢Õß·∫≈ß§å, SD_blank =§à“‡∫’Ë¬ß‡∫π¡“μ√∞“π¢Õß·∫≈ß§å ·≈–

slope=§«“¡™—π¢Õß°√“ø¡“μ√∞“π) ·≈–§à“¢’¥®”°—¥μË” ÿ¥¢Õß°“√«‘‡§√“–Àåª√‘¡“≥ª√Õ∑ (Quantification limit, LOQ) ¡’§à“‡∑à“°—∫ 5.0Ó10^-6 M (n=5) ´÷Ëß§”π«≥®“° (X_blank+10SD_blank)/slope §à“§«“¡

‡∑’Ë¬ß¢Õß°“√μ√«®«—¥ª√Õ∑«—¥‰¥â®“°§à“‡∫’Ë¬ß‡∫π¡“μ√∞“π —¡æ—∑∏å (Relative standard deviation,

%RSD) ¢Õß “√≈–≈“¬ Hg²⁺ ∑’Ë 2 §«“¡‡¢â¡¢âπ §◊Õ 5.0Ó10^-5 M ·≈– 1.0Ó10^-4 M ¡’§à“‡∑à“°—∫

2.84% ·≈– 1.22% (n=5) μ“¡≈”¥—∫ §à“§«“¡‡∑’Ë¬ßπâÕ¬°«à“ 5% · ¥ß«à“‡∑§π‘§π’È¡’§«“¡‡∑’Ë¬ß„π°“√

«‘‡§√“–Àåª√Õ∑ °“√‡ª√’¬∫‡∑’¬∫™à«ß§«“¡‡¢â¡¢âπ¢Õß°“√«‘‡§√“–Àå (Linear range) ·≈–§à“ LOD ¢Õß

‡∑§π‘§π’È°—∫ß“π«‘®—¬Õ◊ËπÊ · ¥ß¥—ßμ“√“ß∑’Ë 1 æ∫«à“ ‡∑§π‘§π’È¡’™à«ß§«“¡‡¢â¡¢âπ¢Õß°“√«‘‡§√“–Àå∑’Ë°«â“ß°«à“

·≈–¡’§à“ LOD μË”°«à“‡∑§π‘§Õ◊ËπÊ

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√Ÿª∑’Ë 6 °√“ø¡“μ√∞“π¢Õß “√≈–≈“¬º ¡√–À«à“ß “√≈–≈“¬ Hg²⁺ ‡¢â¡¢âπ 3.0Ó10^-6 M-1.0Ó10^-3 M

·≈– “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™‡¢â¡¢âπ 2.0 Ó10^-3 M ∑’Ë pH 4 ‚¥¬«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm

μ“√“ß∑’Ë 1 ™à«ß§«“¡‡¢â¡¢âπ Hg²⁺ ·≈–§à“ LOD ¢Õß‡∑§π‘§«‘‡§√“–Àåμà“ßÊ

μ—«μ√«®«—¥ Hg²⁺ Linear Range Limit of Detection Ref.

Azomethine H 3.0Ó10^-6-1.0Ó10^-3 M 3.0Ó10^-6 M This work

Terphenyl derivative 0.1-30 _μM - 9

Thiooxorhodamine 0.13-0.35 _μM - 10

2,3-diferrocenylquinoxaline - 1.3Ó10^-5M 12

4-(2-pyridylazo)-resorcinol 0.35-7.4 _μg/mL 4.49 _μg/mL 13

Benzothiazolium Hemicyanine - 100 ppb 14

benzimidazole substituted - 0.77 _μM 19

boron-dipyrromethene

2-hydroxy 1-napthaldehyde 0.004-0.048 _μg/mL - 20

isonicotinoyl hydrazone

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§«“¡®”‡æ“–„π°“√μ√«®«—¥ª√Õ∑

‡¡◊ËÕπ” “√≈–≈“¬º ¡√–À«à“ß “√≈–≈“¬ Hg²⁺ ‡¢â¡¢âπ 4.0 Ó10^-5 M ·≈–‡Õ‚´‡¡∑’π‡Õ™

‡¢â¡¢âπ 8.0Ó10^-5 M ·≈–‡μ‘¡ “√≈–≈“¬ Cu²⁺, Fe²⁺, Mn²⁺, Ag⁺, Ni²⁺ ·≈– Co²⁺ ‡¢â¡¢âπ 2Ó10^-4 M

∑’≈–™π‘¥ ª√—∫ pH 4 ¥â«¬Õ–´‘‡μ∑∫—ø‡øÕ√å ·≈â«π”‰ª«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm º≈°“√∑¥≈Õß

· ¥ß¥—ß√Ÿª∑’Ë 7 æ∫«à“ ’‡À≈◊Õß¢Õß “√≈–≈“¬º ¡√–À«à“ß Hg²⁺ °—∫‡Õ‚´‡¡∑’π‡Õ™‰¡à‡ª≈’Ë¬π·ª≈ß

‡¡◊ËÕ‡μ‘¡‰ÕÕÕπμà“ßÊ ·μà§à“°“√¥Ÿ¥°≈◊π· ß‡æ‘Ë¡¢÷Èπ‡≈Á°πâÕ¬ ·≈–æ∫«à“‡¡◊ËÕ‡μ‘¡ “√≈–≈“¬ Fe²⁺ ®–∑”„Àâ§à“

°“√¥Ÿ¥°≈◊π· ß‡æ‘Ë¡¢÷Èπ¡“°°«à“‰ÕÕÕπ™π‘¥Õ◊Ëπ ¥—ßπ—Èπ∂â“ “√≈–≈“¬μ—«Õ¬à“ß¡’ Fe²⁺ „πª√‘¡“≥¡“°®”‡ªìπ μâÕß°”®—¥ÕÕ°°àÕπ∑’Ë®–«‘‡§√“–Àåª√Õ∑·≈–¬—ßæ∫«à“‡¡◊ËÕ‡μ‘¡ “√≈–≈“¬ Cu²⁺ §à“°“√¥Ÿ¥°≈◊π· ß®–≈¥≈ß

√Ÿª∑’Ë 7 º≈¢Õß‚≈À–‰ÕÕÕπ™π‘¥μà“ßÊ μàÕ°“√μ√«®«—¥ª√Õ∑ (a) ‡ª°μ√“°“√¥Ÿ¥°≈◊π· ß¢Õß “√≈–≈“¬º ¡√–À«à“ß “√≈–≈“¬ Hg²⁺ 4.0Ó10^-5 M ·≈– “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™‡¢â¡¢âπ 8.0Ó10^-5 M ‡¡◊ËÕ‡μ‘¡ “√≈–≈“¬ Cu²⁺, Fe²⁺, Mn²⁺, Ag⁺, Mn²⁺, Ni²⁺ ·≈– Co²⁺ ‡¢â¡¢âπ 2.0Ó10^-4 M ·≈– (b) °√“ø§à“¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm ¢Õß “√≈–≈“¬∑’Ë ¿“«–‡¥’¬«°—π

°“√μ√«® Õ∫§«“¡∂Ÿ°μâÕß¢Õß‡∑§π‘§„π°“√μ√«®«—¥ª√‘¡“≥ Hg²⁺ „ππÈ”ª√–ª“

‡¡◊ËÕ∑”°“√ª√–‡¡‘π‡∑§π‘§«‘‡§√“–Àåª√Õ∑„ππÈ”ª√–ª“‚¥¬°“√‡μ‘¡ “√≈–≈“¬¡“μ√∞“π Hg²⁺

∑’Ë 2 §«“¡‡¢â¡¢âπ§◊Õ 8.0Ó10^-5 M ·≈– 2.0Ó10^-4 M ·≈– “√≈–≈“¬‡Õ‚´‡¡∑’π‡Õ™‡¢â¡¢âπ 4.0Ó10^-4 M

≈ß„πμ—«Õ¬à“ßπÈ”ª√–ª“ ·≈–ª√—∫ pH ‡∑à“°—∫ 4 ¥â«¬Õ–´‘‡μ∑∫—ø‡øÕ√å ·≈â«π”‰ª«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë 425.5 nm º≈°“√∑¥≈Õß· ¥ß¥—ßμ“√“ß∑’Ë 2 æ∫«à“ §à“√âÕ¬≈–°“√§◊π°≈—∫ (%Recovery) ¡’§à“‡∑à“°—∫

94% ·≈– 103% μ“¡≈”¥—∫ ´÷Ëß‡ªìπ§à“∑’Ë¬Õ¡√—∫‰¥â· ¥ß„Àâ‡ÀÁπ«à“‡∑§π‘§π’È “¡“√∂π”¡“ª√–¬ÿ°μå„™â„π

°“√«‘‡§√“–Àåª√Õ∑„ππÈ”‰¥â

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μ“√“ß∑’Ë 2 °“√«‘‡§√“–Àå Hg²⁺ „ππÈ”ª√–ª“

μ—«Õ¬à“ß [Hg²⁺] ∑’Ë‡μ‘¡ (M) [Hg²⁺]_±SD ∑’Ëæ∫ (M) % Recovery

πÈ”ª√–ª“ 8.00Ó10^-5 (7.56_±0.66)Ó10^-5 94

2.00Ó10^-4 (2.09_±0.05)Ó10^-4 103

√ÿª·≈–«‘®“√≥åº≈°“√∑¥≈Õß

‡Õ‚´‡¡∑’π‡Õ™ (L) “¡“√∂„™â‡ªìπμ—«μ√«®«—¥ª√‘¡“≥ Hg²⁺ ‰¥â¥’ ‡π◊ËÕß®“°«‘∏’μ√«®«—¥‰¡à

´—∫´âÕπ¡’§«“¡®”‡æ“–‡®“–®ß„π°“√μ√«®«—¥§àÕπ¢â“ß¡“° ‰¡à‰¥â√—∫º≈°√–∑∫®“°‰ÕÕÕπ¢Õß‚≈À–™π‘¥Õ◊ËπÊ

‡™àπ Cu²⁺, Mn²⁺, Ag⁺, Ni²⁺ ·≈– Co²⁺ ¬°‡«âπ Fe²⁺ ´÷Ëß “¡“√∂√∫°«π°“√«‘‡§√“–Àå‰¥âÀ“°¡’ªπ‡ªóôÕπ

„πª√‘¡“≥¡“° ‡Õ‚´‡¡∑’π‡Õ™∑”ªØ‘°‘√‘¬“°—∫ Hg²⁺ „πÕ—μ√“ à«π 1 : 1 ‡°‘¥‡ªìπ “√ª√–°Õ∫‡™‘ß´âÕπ¢Õß ª√Õ∑ (HgL) ¡’ ’‡À≈◊Õß∑—π∑’´÷Ëß —ß‡°μ‡ÀÁπ‰¥â¥â«¬μ“‡ª≈à“·≈–„Àâ ’∑’Ë§ß∑π “¡“√∂§ß ’Õ¬Ÿà‰¥âπ“π°«à“

12 ™—Ë«‚¡ß ¡’§«“¡‰«„π°“√«‘‡§√“–Àå·≈– “¡“√∂μ√«®«‘‡§√“–Àå Hg²⁺ ‰¥â¥’„π™à«ß 3.0Ó10^-6-1.0Ó10^-3 M

¡’§à“ LOD=3.0Ó10^-6 M ·≈–§à“ LOQ =5.0Ó10^-6 M „™âμ—«∑”≈–≈“¬‡ªìππÈ”∑”„Àâ§à“„™â®à“¬„π°“√

«‘‡§√“–Àå‰¡à Ÿß·≈–‰¡à‡ªìπæ‘…μàÕ ‘Ëß·«¥≈âÕ¡‡π◊ËÕß®“°‰¡à„™âμ—«∑”≈–≈“¬Õ‘π∑√’¬å “¡“√∂π”‰ªª√–¬ÿ°μå„™â„π

°“√«‘‡§√“–Àå Hg²⁺ „πμ—«Õ¬à“ßπÈ”‰¥â¥’

‡Õ° “√Õâ“ßÕ‘ß

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Liquid Chromatography with Precolumn Complexation Reaction using Salicylaldehyde and 1-Amino-8-naphthol-3,6-disulfonate. Anal. Bioanal. Chem. 391: 1101-1106.

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‰¥â√—∫∫∑§«“¡«—π∑’Ë 25 ‡¡…“¬π 2559

¬Õ¡√—∫μ’æ‘¡æå«—π∑’Ë 19 æƒ…¿“§¡ 2559

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