Dendrobium friedericksianum Rchb. f. and Dendrobium crumenatum Sw.

ýċÖþćÿĎêøĂćĀćøìĊęđĀöćąÿöĔîÖćø×÷ć÷óĆîíčŤĀüć÷đĀúČĂÜÝĆîìïĎøĒúąĀüć÷êąöĂ÷

ēé÷ÖćøđóćąđúĊĚ÷ÜđîČĚĂđ÷ČęĂóČß

ÿčõćõøèŤ ÿćßćêĉ^1*, ÷øø÷Ü óĆîíŤóùÖþŤ² Ēúą ýýĉöć đöČĂÜĒÖšü³

1ÿëćïĆîüĉÝĆ÷óČßÿüî ÖøöüĉßćÖćøđÖþêø, ÖøčÜđìóöĀćîÙø

2ýĎî÷ŤđìÙēîēú÷ĊÿćøÿîđìýĒúąÖćøÿČęĂÿćø ÖøöüĉßćÖćøđÖþêø, ÖøčÜđìóöĀćîÙø

3ýĎî÷ŤüĉÝĆ÷óČßÿüîÝĆîìïčøĊ ÿëćïĆîüĉÝĆ÷óČßÿüî ÖøöüĉßćÖćøđÖþêø, ÝĆîìïčøĊ

*sachati08@hotmail.com ïìÙĆé÷ŠĂ

ÖćøýċÖþćÿĎêøĂćĀćøìĊęđĀöćąÿöĔîÖćø×÷ć÷óĆîíčŤĀüć÷đĀúČĂÜÝĆîìïĎøĒúąĀüć÷êąöĂ÷ēé÷ÖćøđóćąđúĊĚ÷ÜđîČĚĂđ÷ČęĂ õć÷ĔêšÖĉÝÖøøö ÖćøñúĉêÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎøĒúąĀüć÷êąöĂ÷đóČęĂđðŨîÿöčîĕóøìćÜÖćøÙšć đøĉęöêšîđéČĂîêčúćÙö 2561 ÿĉĚîÿčéđéČĂîÖĆî÷ć÷î 2564 öĊüĆêëčðøąÿÜÙŤđóČęĂĔĀšĕéšüĉíĊÖćø×÷ć÷óĆîíčŤĔîÿõćóðúĂéđßČĚĂÿĞćĀøĆïÖćøñúĉêÖúšü÷ĕöšìĊęöĊýĆÖ÷õćóđðŨî ÿöčîĕóø ðøąÖĂïéšü÷ 2 ×ĆĚîêĂî ÙČĂ ÖćøßĆÖîĞćĔĀšđÖĉéêšîĒúąÖćøđóĉęöðøĉöćè ĒúąÖćøßĆÖîĞćĔĀšđÖĉéøćÖĒúąÖćø÷šć÷Ăîčïćú ÖćøßĆÖ îĞćĔĀšđÖĉéêšîĒúąÖćøđóĉęöðøĉöćèĔîÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ĂćĀćøìĊęđĀöćąÿöìĊęÿčé ÙČĂĂćĀćøÿĎêø MS ìĊęđêĉö BA 5 öĉúúĉÖøĆöêŠĂúĉêøĔĀšÝĞćîüîĀîŠĂöćÖìĊęÿčéÙČĂ 3.4 ĀîŠĂ đöČęĂđúĊĚ÷Üîćî 60 üĆî ÿŠüîÖúšü÷ĕöšĀüć÷êąöĂ÷ ÙČĂĂćĀćøÿĎêø VW ìĊę ðøćýÝćÖÿćøÙüïÙčöÖćøđÝøĉâđêĉïēê BA ÝĞćîüîĀîŠĂöćÖìĊęÿčé 6.1 ĀîŠĂ đöČęĂđúĊĚ÷Üîćî 90 üĆî ÿŠüîÖćøßĆÖîĞćĔĀšđÖĉéøćÖ ĂćĀćøìĊęđĀöćąÿöĔîÖćøßĆÖîĞćĔĀšđÖĉéøćÖ×ĂÜÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ĒúąĀüć÷êąöĂ÷ ÙČĂĂćĀćøÿĎêø MS ìĊęđêĉö NAA 0.5 öĉúúĉÖøĆöêŠĂúĉêø öĊðøĉöćèøćÖéĊìĊęÿčéÙČĂ 10.4 Ēúą 4.0 øćÖ đöČęĂđúĊĚ÷Üîćî 90 Ēúą 60 üĆî êćöúĞćéĆï

ÙĞćÿĞćÙĆâ: Öúšü÷ĕöšÿöčîĕóø Āüć÷đĀúČĂÜÝĆîìïĎø Āüć÷êąöĂ÷ ÖćøđÖĉéêšî ÖćøđÖĉéøćÖ

Study on applicable medium in micropropagation of

Dendrobium friedericksianum Rchb. f. and Dendrobium crumenatum Sw.

Supaporn Sachati^1*, Yanyong Phanpruek² and Sasima Muangkaew³

1Horticultural Research Institute Department of Agriculture, Bangkok

2Information and Communication Technology Center Department of Agriculture, Bangkok

3Chanthaburi Horticultural Research Center Horticultural Research Institute Department of Agriculture, Chanthaburi

*sachati08@hotmail.com Abstract

The study of suitable formula for propagation of Dendrobium friedericksianum Rchb. f. and D.

crumenatum Sw. by tissue culture under the activity production of D. friedericksianum Rchb. f. and D.

crumenatum Sw. for commercial medicinal, purposes beginning of October 2018 and ending of September 2021. The objective is to obtain a sterile propagation method for the production of orchids with medicinal potential, consisting of 2 steps: plant induction and proliferation, and root induction and nursery migration. In Induction and proliferation in D. friedericksianum Rchb. f. step, the optimal MS medium combined with 5 mg/L of BA for the maximum number of shoots was 3.4 shoots after culturing for 60 days.

While the D. crumenatum Sw. was the VW medium without BA growth regulator, the maximum number of shoots was 6.1 after culturing for 90 days. For the medium of root induction of D. friedericksianum Rchb. f.

and D. crumenatum Sw. were MS medium combined with 0.5 mg/L of NAA. The maximum amount of root were 10.4 and 4.0 roots after 90 and 60 days of cultivation, respectively.

Keywords: herbal orchid, Dendrobium friedericksianum Rchb. f., D. crumenatum Sw., shoot induction, root induction

1. ïìîĞć

Öúšü÷ĕöšĔîÿÖčú Dendrobium đðŨîĀîċęÜĔîÿÖčúÖúšü÷ĕöšìĊęëĎÖÙšîóïÿć÷óĆîíčŤëċÜ 1,100 ßîĉé àċęÜÖøąÝć÷Ă÷ĎŠĔîìüĊð đĂđßĊ÷ ÷čēøð ĒúąĂĂÿđêøđúĊ÷ [1] Ĕîðøąđìýĕì÷öĊöćÖÖüŠć 150 ßîĉé [2] Öúšü÷ĕöšđĀúŠćîĊĚöĊÿćøôŘîĂú (phenol) ĔîēÙøÜÿøšćÜ ĕéšĒÖŠ bibenzyl, phenanthrene Ēúą fluorenone đðŨîĂÜÙŤðøąÖĂïĀúĆÖ [3] ÖćøýċÖþćìćÜđõÿĆßüĉì÷ćĒÿéÜĔĀšđĀĘîüŠćÿćø ÖúčŠöôŘîĂú ēé÷đÞóćą moscatilin öĊùìíĉĝìćÜßĊüõćóìĊęĀúćÖĀúć÷ đߊî ùìíĉĝêšćîÖćøĂĆÖđÿï (anti-inflammation) ùìíĉĝêšćî ĂîčöĎúĂĉÿøą (antioxidant) ùìíĉĝêšćîÖćøđÝøĉâ×ĂÜĀúĂéđúČĂé (anti-angiogenesis) [4-6]

ðŘ ó.ý. 2559 ÖćøüĉÝĆ÷ĀćðøĉöćèÿćøðøąÖĂïôŘîĂúĂĊÖ 9 ßîĉé ĕéšĒÖŠ (2S)-eriodictyol, (2S)-homoeriodictyol, dendroflorin, moscatilin, lusianthridin, gigantol, nobilone, chrysotoxine Ēúą crepidatin (õćóìĊę 1) ĔîÖúšü÷ĕöš ÿÖčú Dendrobium ÝĞćîüî 23 ßîĉé óïüŠćĀüć÷êąöĂ÷öĊÿćøìĆĚÜ 9 ßîĉé ēé÷óï dendroflorin, moscatilin Ēúą

lusianthridin öćÖìĊęÿčé 0.0433, 0.0834 Ēúą 0.0079 đðĂøŤđàĘîêŤ w/w êćöúĞćéĆï Āüć÷đĀúČĂÜÝĆîìøïĎøìĊęđÖĘïÝćÖìćÜ õćÙđĀîČĂ óïÿćø (2S)-eriodictyol Ēúą (2S)-homoeriodictyol öćÖìĊęÿčé 0.0549 Ēúą 0.0425 đðĂøŤđàĘîêŤ w/w êćöúĞćéĆï ĒúąÿćøÿĞćÙĆâïćÜßîĉéĔîđĂČĚĂÜÙĞćðŝĂÖ đĂČĚĂÜĒàąĀöŠî Āüć÷ÝĊî đĂČĚĂÜîĉĚüöČĂßąîĊ ĒúąĂČęî [7]

Öúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø (Dendrobium friedericksianum Rchb. f.) đðŨîÖúšü÷ĕöšðśćóĆîíčŤóČĚîđöČĂÜ×ĂÜ ðøąđìýĕì÷öĊđ×êÖøąÝć÷óĆîíčŤĂ÷ĎŠĔîðśćéÜéĉïìćÜõćÙêąüĆîĂĂÖĒëïÝĆÜĀüĆéÝĆîìïčøĊĒúąêøćé [8] Öúšü÷ĕöšĀüć÷đĀúČĂÜ ÝĆîìïĎøÝĆéđðŨîóČßĂîčøĆÖþŤïĆâßĊìĊę 2 êćöĂîčÿĆââćüŠćéšü÷ÖćøÙšćøąĀüŠćÜðøąđìýàċęÜßîĉéÿĆêüŤðśćĒúąóČßðśćìĊęĔÖúšÿĎâóĆîíčŤđðŨî óČßĂîčøĆÖþŤĔîĂîčÿĆââćĕàđêÿ (CITES: Convention on International Trade In Endangered Species of Wild Fauna and Flora)

Öúšü÷ĕöšĀüć÷êąöĂ÷ (Dendrobium crumenatum Sw.) öĊúĆÖþèąđðŨîÖúšü÷ĕöšĂĉÜĂćýĆ÷ ēÙîêšîđðŨîúĞćúĎÖÖúšü÷

ìøÜÖøąïĂÖ ÿŠüîðúć÷đðŨîđÿšîÖúöđøĊ÷üĒúąĒ×ĘÜ ÷ćüĕéšëċÜ 70 đàîêĉđöêø ĔïøĎðøĊĒÖö×Ăï×îćî ÖüšćÜ 1.5-2.5 đàîêĉđöêø ÷ćü 5-7 đàîêĉđöêø öĆÖìĉĚÜĔïđöČęĂñúĉéĂÖ éĂÖđéĊę÷üĂĂÖêćöךà ÿĊ×ćüîüú ïćîđêĘöìĊę ÖüšćÜ 2.5-3 đàîêĉđöêø öĊÖúĉęîĀĂö ÖúĊïðćÖöĊĒêšöÿĊ đĀúČĂÜìĊęÖúćÜÖúĊï ÖćøÖøąÝć÷óĆîíčŤóïĕéšìĆęüðøąđìý

ÿćøÿĞćÙĆâĀøČĂÿćøĂĂÖùìíĉĝìĊęóïĔîóČßÿöčîĕóøìĊęëĎÖîĞćöćĔßšðøąē÷ßîŤîĆĚî ÿŠüîĔĀâŠđðŨîÖúčŠöÿćøđöìćēïĕúìŤìčêĉ÷õĎöĉ ðøĉöćèÖćøÿĆÜđÙøćąĀŤÿćøéĆÜÖúŠćüÝċÜ×ċĚîĂ÷ĎŠÖĆïðŦÝÝĆ÷ìĊęđÖĊę÷üךĂÜÖĆïÿćøךćÜêšîéšü÷ ðŦÝÝĆ÷êŠćÜ ė ìĆĚÜðŦÝÝĆ÷õć÷îĂÖĒúąðŦÝÝĆ÷

õć÷ĔîìĊęöĊñúêŠĂÖćøđÝøĉâđêĉïēê ñúñúĉêĒúąĂÜÙŤðøąÖĂïìćÜđÙöĊ×ĂÜóČßÿöčîĕóø öĊïìïćìÿĞćÙĆâêŠĂÖćøĀćĒîüìćÜĔîÖćø đóĉęöñúñúĉêìĊęđðŨîÿćøĂĂÖùìíĉĝ (ßîĉéĒúąðøĉöćèÿćøĂĂÖùìíĉĝ) ĒúąñúñúĉêìĊęđðŨîöüúßĊüõćó (îĚĞćĀîĆÖĒĀšÜêŠĂîĚĞćĀîĆÖÿé) ×ĂÜ óČßÿöčîĕóøĔĀšÿĎÜ×ċĚî ĒúąđóĊ÷ÜóĂêŠĂÖćøîĞćöćĔßšðøąē÷ßîŤĔîđßĉÜđýøþåÖĉÝ ÝćÖÖćøýċÖþćëċÜÿćøÿĞćÙĆâĔîÖúšü÷ĕöšĀüć÷đĀúČĂÜ ÝĆîìïĎøĒúąĀüć÷êąöĂ÷ àċęÜĕéšýċÖþćÿĞćøüÝ øüïøüö ĒúąöĊÖćøîĞćÖúšü÷ĕöšÿöčîĕóøđĀúŠćîĆĚîöćĔßšðøąē÷ßîŤ ÝċÜÙüøöĊÖćø óĆçîćĒúą×÷ć÷óĆîíčŤĔîÿõćóðúĂéđßČĚĂÖĆïÖúšü÷ĕöšĀüć÷ìĆĚÜ 2 ßîĉéîĊĚ đóČęĂÖćøñúĉêÖúšü÷ĕöšìĊęöĊýĆÖ÷õćóđðŨîÿöčîĕóøĔîđßĉÜ ÖćøÙšć

2. üĉíĊéĞćđîĉîÖćø

2.1 ×ĆĚîêĂîÖćøßĆÖîĞćĔĀšđÖĉéêšîĒúąÖćøđóĉęöðøĉöćè

îĞćĀîŠĂĂŠĂîÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ĒúąĀüć÷êąöĂ÷ öćìĞćÖćøôĂÖÛŠćđßČĚĂ ēé÷đßĘéìĞćÙüćöÿąĂćéĒúąúĂÖ ÖćïîĂÖĂĂÖ ôĂÖÛŠćđßČĚĂéšü÷ĒĂúÖĂăĂúŤ 70 đðĂøŤđàĘîêŤ îćî 1 îćìĊ êćöéšü÷ NaOCl 1.6 đðĂøŤđàĘîêŤ îćî 15 îćìĊ Ēúą NaOCl 0.4 đðĂøŤđàĘîêŤ îćî 10 îćìĊ êćöúĞćéĆï úšćÜéšü÷îĚĞćÖúĆęîîċęÜÛŠćđßČĚĂ 3 ÙøĆĚÜ úĂÖÖćïĔîêĎšðúĂéđßČĚĂÝîđĀúČĂÝčéđÝøĉâ ĒúąđóćąđúĊĚ÷ÜïîĂćĀćøÿĎêøßĆÖîĞćĔĀšđÖĉéêšîĂŠĂîĒúąđóĉęöðøĉöćèêćöÖøøöüĉíĊìĊęÖĞćĀîé üćÜĒñîÖćøìéúĂÜĒïïÿčŠöÿöïĎøèŤ (CRD) ÝĞćîüî 10 àĚĞć (×üé) ĔßšÿĎêøĂćĀćøđðŨîÖøøöüĉíĊ ÙČĂĂćĀćøÿĎêø MS Ēúą VW øŠüöÖĆïÿćøÙüïÙčöÖćøđÝøĉâđêĉïēê BA Ùüćöđךöךî 0, 5, 10 Ēúą 15 öĉúúĉÖøĆöêŠĂúĉêø îĞćĕðđúĊĚ÷ÜïîßĆĚîìĊęöĊĒÿÜÿüŠćÜðøąöćè 1,000-3,000 úĆÖàŤ ìĊęĂčèĀõĎöĉ 25-27 ĂÜýćđàúđàĊ÷ÿ ïĆîìċÖךĂöĎúÖćøðîđðŚŪĂîđßČĚĂ ĒúąÖćøđóĉęöðøĉöćè×ĂÜêšîĂŠĂîìĊęĂć÷č 30, 60 Ēúą 90 üĆî

2.2 ×ĆĚîêĂîÖćøßĆÖîĞćĔĀšđÖĉéøćÖ

îĞćêšîĂŠĂîÝćÖ×ĆĚîêĂîÖćøßĆÖîĞćĔĀšđÖĉéêšîĒúąÖćøđóĉęöðøĉöćè öćđóćąđúĊĚ÷ÜêćöÖøøöüĉíĊìĊęÖĞćĀîé ēé÷êšîĂŠĂîêšĂÜ öĊÙüćöÿĎÜĂ÷ŠćÜîšĂ÷ 1-2 đàîêĉđöêø üćÜĒñîÖćøìéúĂÜĒïïÿčŠöÿöïĎøèŤ (CRD) ÝĞćîüî 10 àĚĞć (×üé) ĔßšÿĎêøĂćĀćøđðŨî ÖøøöüĉíĊ ÙČĂĂćĀćøÿĎêø MS Ēúą VW øŠüöÖĆïÿćøÙüïÙčöÖćøđÝøĉâđêĉïēê NAA Ùüćöđךöךî 0, 0.5, 0.1 Ēúą 1.5 öĉúúĉÖøĆö êŠĂúĉêø îĞćĕðüćÜïîßĆĚîìĊęöĊĒÿÜÿüŠćÜðøąöćè 1,000-3,000 úĆÖàŤ ìĊęĂčèĀõĎöĉ 25-27 ĂÜýćđàúđàĊ÷ÿ ïĆîìċÖךĂöĎúÖćøðîđðŚŪĂî đßČĚĂ Öćøđóĉęöðøĉöćè×ĂÜøćÖìĊęĂć÷č 30, 60 Ēúą 90 üĆî ĀúĆÜÝćÖîĆĚîìĞćÖćø÷šć÷ÖúšćĂîčïćúĔîđøČĂîđóćąßĞć ïĆîìċÖךĂöĎúÖćø øĂéßĊüĉêĀúĆÜ÷šć÷ðúĎÖ ÖćøüĉđÙøćąĀŤ×šĂöĎúđßĉÜÿëĉêĉ îĞćךĂöĎúìĊęĕéšÝćÖÖćøøüïøüööćüĉđÙøćąĀŤÙŠćÙüćöĒðøðøüî Ēúą

đðøĊ÷ïđìĊ÷ïÙŠćđÞúĊę÷éšü÷üĉíĊ Duncan’s New Multiple Range Test (DMRT) ÝćÖēðøĒÖøöÿĞćđøĘÝøĎðìćÜÿëĉêĉ IRRISTAT for Dos ēé÷ĔßšĀúĆÖĔîÖćøðøąđöĉîìĊęøąéĆïÙüćöđßČęĂöĆęî 95% ÿëćîìĊęĒúąøą÷ąđüúćĔîÖćøìéúĂÜ ÙČĂđøĉęöêšî êčúćÙö 2561 – ÿĉĚîÿčé ÖĆî÷ć÷î 2564 è ĀšĂÜðäĉïĆêĉÖćøđóćąđúĊĚ÷ÜđîČĚĂđ÷ČęĂóČß ÿëćïĆîüĉÝĆ÷óČßÿüî ÖøöüĉßćÖćøđÖþêø

3. ñúÖćøìéúĂÜĒúąüĉÝćøèŤ 3.1 ÖćøßĆÖîĞćĔĀšđÖĉéêšîĒúąÖćøđóĉęöðøĉöćè

ĔîÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ĒúąĀüć÷êąöĂ÷ óïüŠćĕöŠöĊÝĞćîüîĀîŠĂìĊęđóĉęö×ċĚîđöČęĂđúĊĚ÷Üîćî 30 üĆî àċęÜĔî Öúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎøìĊęđúĊĚ÷Üîćî 60 Ēúą 90 üĆî öĊÝĞćîüîĀîŠĂìĊęđóĉęö×ċĚîĕöŠĒêÖêŠćÜÖĆîöćÖîĆÖ éĆÜîĆĚîĂćĀćøìĊęđĀöćąÿö ĔîÖćøđóĉęöðøĉöćèÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ÙČĂĂćĀćøÿĎêø MS ìĊęđêĉö BA 5 öĉúúĉÖøĆöêŠĂúĉêø ĔĀšÝĞćîüîĀîŠĂöćÖìĊęÿčéÙČĂ 3.4 ĀîŠĂ đöČęĂđúĊĚ÷Üîćî 60 üĆî ÿŠüîÖúšü÷ĕöšĀüć÷êąöĂ÷đöČęĂđúĊĚ÷Üîćî 90 üĆî ÝąĔĀšðøĉöćèĀîŠĂöćÖÖüŠćìĊęđúĊĚ÷Üîćî 60 üĆî àċęÜĂćĀćøìĊęđĀöćąÿöìĊęÿčé ÙČĂĂćĀćøÿĎêø VW ìĊęðøćýÝćÖÿćøÙüïÙčöÖćøđÝøĉâđêĉïēê BA öĊÝĞćîüîĀîŠĂöćÖìĊęÿčé 6.1 ĀîŠĂ đöČęĂđúĊĚ÷Üîćî 90 üĆî (êćøćÜìĊę 1)

êćøćÜìĊę 1 ÖćøđóĉęöÝĞćîüîĀîŠĂđÞúĊę÷×ĂÜÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ĒúąĀüć÷êąöĂ÷ìĊęđúĊĚ÷Üîćî 60 Ēúą 90 üĆî Ĕî ĂćĀćøÿĎêøêŠćÜ ė

ÿĎêøĂćĀćø đĀúČĂÜÝĆîìïĎø (ĀîŠĂ) Āüć÷êąöĂ÷ (ĀîŠĂ)

60 üĆî 90 üĆî 60 üĆî 90 üĆî

MS 2.8^ab 3.0^a 2.7^ab 3.4^cde

MS ìĊęđêĉö BA 5 mg/L 3.4^a 3.1^a 3.6^a 5.9^ab

MS ìĊęđêĉö BA 10 mg/L 1.7^b 1.9^c 3.2^a 4.8^abc

MSìĊęđêĉö BA 15mg/L 2.6^ab 2.3^abc 2.8^ab 4.5^bcd

VW 2.4^ab 2.0^bc 3.0^ab 6.1^a

VW ìĊęđêĉö BA 5mg/L 2.9^ab 2.9^ab 2.1^b 2.8^e

VW ìĊęđêĉö BA 10mg/L 2.0^b 1.8^c 2.1^b 3.1^de

VW ìĊęđêĉö BA 15mg/L 2.2^ab 2.3^abc 1.0^c 1.3^f

Āöć÷đĀêč: êĆüđú×ìĊęêćöéšü÷ĂĆÖþøìĊęđĀöČĂîÖĆî ĕöŠöĊÙüćöĒêÖêŠćÜÖĆîìćÜÿëĉêĉìĊęøąéĆïÙüćöđßČęĂöĆęî 95 đðĂøŤđàĘîêŤ (p<0.05) ēé÷üĉíĊ DMRT

ÝćÖÖćøýċÖþćÖćøđóĉęöÝĞćîüîĀîŠĂđÞúĊę÷×ĂÜÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎøĒúąĀüć÷êąöĂ÷ ĀúĆÜđóćąđúĊĚ÷ÜïîĂćĀćøÿĎêø ÿĆÜđÙøćąĀŤ îćî 60 Ēúą 90 üĆî óïüŠćÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ÖćøđóĉęöÝĞćîüîĀîŠĂđÞúĊę÷ÿĎÜÿčéđöČęĂđóćąđúĊĚ÷Üîćî 60 üĆî Ēúą óïĀîŠĂĀøČĂ÷ĂéĔĀöŠ browning êć÷đöČęĂđúĊĚ÷Üîćî 90 üĆî Ĕî×èąìĊęÖúšü÷ĕöšĀüć÷êąöĂ÷ ÖćøđóĉęöÝĞćîüîĀîŠĂđÞúĊę÷đóĉęö×ċĚî đöČęĂ đúĊĚ÷Üîćî 90 üĆî àċęÜĒêÖêŠćÜÖĆïøć÷ÜćîñúÖćøìéúĂÜ×ĂÜîć÷ĉÖć [9] üŠćÖćøđóĉęöÝĞćîüî÷ĂéøüöÿĎÜ×ċĚî×ĂÜÖúšü÷ĕöšĀüć÷đĀúČĂÜ ÝĆîìïĎø ĔîÿĎêøĂćĀćø MS đöČęĂđúĊĚ÷Üîćî 30, 60 Ēúą 90 üĆî ēé÷ĔĀšÝĞćîüî÷ĂéđÞúĊę÷ìĊę 3.01, 3.32 Ēúą 3.89 ÷ĂéêŠĂßĉĚîÿŠüî êćöúĞćéĆï

ĒúąÝćÖñú×ĂÜÿĎêøĂćĀćøêŠĂÖćøđÝøĉâđêĉïēê×ĂÜÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎøĒúąĀüć÷êąöĂ÷ ēé÷ÖćøđóćąđúĊĚ÷Ü đðøĊ÷ïđìĊ÷ïïîĂćĀćøÿĎêøÿĆÜđÙøćąĀŤ MS Ēúą VW óïüŠćÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎøđöČęĂđúĊĚ÷ÜĔîĂćĀćøÿĎêø MS ìĊę ðøćýÝćÖÿćøÙüïÙčöÖćøđÝøĉâđêĉïēê BA ĒúąìĊęđêĉö BA ÿŠÜđÿøĉöÖćøđÖĉéĀîŠĂđÞúĊę÷ĕéšÿĎÜÖüŠćĂćĀćøÿĎêø VW ÷ÖđüšîđÞóćą

Öúšü÷ĕöšĀüć÷êąöĂ÷đöČęĂđúĊĚ÷ÜĔîĂćĀćøÿĎêø VW ìĊęðøćýÝćÖÿćøÙüïÙčöÖćøđÝøĉâđêĉïēê ÿŠÜđÿøĉöÖćøđÖĉéĀîŠĂđÞúĊę÷ĕéšÿĎÜÖüŠć ĂćĀćøÿĎêø MS àċęÜ Steward [10] ĕéšđðøĊ÷ïđìĊ÷ïðøĉöćèíćêčĂćĀćøĔîĂćĀćøÿĆÜđÙøćąĀŤ óïüŠćĂćĀćøÿĎêø MS öĊðøĉöćè ĒĂöēöđîĊ÷Ēúąnicotinic acid ÿĎÜÖüŠćĂćĀćøÿĎêø VW ĂćÝÖúŠćüĕéšüŠćĂćĀćøÿĎêøÿĆÜđÙøćąĀŤ MS ÿŠÜđÿøĉöÖćøđÝøĉâđêĉïēêĒúą ÖćøđóĉęöÝĞćîüîĀîŠĂđÞúĊę÷×ĂÜÖúšü÷ĕöšđĀúČĂÜÝĆîìïĎøĔîÖćøđóćąđúĊĚ÷ÜđîČĚĂđ÷ČęĂ àċęÜÿĂéÙúšĂÜÖĆïÜćîüĉÝĆ÷×ĂÜðøĆßóøøè [11] ìĊę ĕéšýċÖþćñú×ĂÜÿĎêøĂćĀćøĒúąÿćøÙüïÙčöÖćøđÝøĉâ×ĂÜÖúšü÷ĕöšđĀúČĂÜÝĆîìïĎøĔîĀúĂéìéúĂÜ óïüŠćÖćøđóćąđúĊĚ÷Üïî ĂćĀćøÿĎêø MS ìĊęðøćýÝćÖÿćøÙüïÙčöÖćøđÝøĉâđêĉïēê ÿćöćøëßĆÖîĞćÖćøđÖĉé÷ĂéøüöđÞúĊę÷ 3.21 ÷ĂéêŠĂßĉĚîÿŠüîĒúąßĆÖîĞć ÖćøđÖĉé÷ĂéøüöĕéšÿĎÜÖüŠćĂćĀćøÿĎêø VW Ēúą îć÷ĉÖć [9] ìĊęĕéšýċÖþćÿĎêøĂćĀćøìĊęöĊñúêŠĂÖćøđÝøĉâĒúąÖćøĂĂÖéĂÖ×ĂÜ Öúšü÷ĕöšđĀúČĂÜÝĆîìïĎø (Dendrobium friedericksianum Rchb. f.) ĔîĀúĂéìéúĂÜ óïüŠćĂćĀćøìčÖÿĎêøĕöŠÿćöćøëßĆÖîĞć ÖćøđÖĉééĂÖĕéš ēé÷ĂćĀćøÿĎêø MS ÿŠÜđÿøĉöÖćøđÖĉé÷ĂéøüöđÞúĊę÷ÿĎÜÿčé 3.89 ÷ĂéêŠĂßĉĚîÿŠüî ĒúąÿĂéÙúšĂÜÖĆïÜćîüĉÝĆ÷ [12- 14] ìĊęìĞćÖĆïÖúšü÷ĕöšđĂČĚĂÜßšćÜîšćüĒúąÖúšü÷ĕöšđĂČĚĂÜÖčĀúćïÖøąđðŞćðŗé

đöČęĂóĉÝćøèćñú×ĂÜÿćøÙüïÙčöÖćøđÝøĉâđêĉïēê BA ĒúąÿĎêøĂćĀćøÿĆÜđÙøćąĀŤìĊęöĊñúêŠĂÖćøđÝøĉâđêĉïēê×ĂÜ Öúšü÷ĕöšđĀúČĂÜÝĆîìïĎøĒúąĀüć÷êąöĂ÷ ēé÷ÿĎêøĂćĀćø MS Ēúą VW ìĊęĕöŠđêĉöĒúąđêĉö BA ìĊęøąéĆïÙüćöđךöךî 5, 10 Ēúą 15 öĉúúĉÖøĆöêŠĂúĉêø óïüŠćÿĎêøĂćĀćø MS øŠüöÖĆï BA ìĊęøąéĆïÙüćöđךöךî 5 öĉúúĉÖøĆöêŠĂúĉêø ÿŠÜđÿøĉöÖćøđÖĉéĀîŠĂđÞúĊę÷

ÿĎÜÿčéÖúšü÷ĕöšđĀúČĂÜÝĆîìïĎøĒúąĀüć÷êąöĂ÷ 3.4 Ēúą 3.6 ĀîŠĂêŠĂßĉĚîÿŠüî ìĊęøą÷ąđüúć 60 üĆî êćöúĞćéĆï ĒúąìĊęøą÷ąđüúć 90 üĆî Öúšü÷ĕöšĀüć÷êąöĂ÷ìĊęđúĊĚ÷ÜĔîÿĎêøĂćĀćø VW ìĊęĕöŠđêĉö BA ÿŠÜđÿøĉöÖćøđÖĉéĀîŠĂđÞúĊę÷ÿĎÜÿčé 6.1 ĀîŠĂêŠĂßĉĚîÿŠüî Ēúą øĂÜúÜöćÙČĂđúĊĚ÷ÜĔîĂćĀćøÿĎêø MS øŠüöÖĆï BA ìĊęøąéĆïÙüćöđךöךî 5 öĉúúĉÖøĆöêŠĂúĉêø ÿŠÜđÿøĉöÖćøđÖĉéĀîŠĂđÞúĊę÷ 5.9 ĀîŠĂ êŠĂßĉĚîÿŠüî ĒúąđöČęĂđêĉöÙüćöđךöךî×ĂÜ BA ĔĀšÿĎÜ×ċĚîĂĆêøćÖćøđÖĉé÷ĂéøüöúéúÜ àċęÜÿĂéÙúšĂÜÖĆïÜćîüĉÝĆ÷×ĂÜ îć÷ĉÖć [9]

óïüŠćĂćĀćøÿĎêø ìĊęđêĉö BA 5 öĉúúĉÖøĆöêŠĂúĉêø ÿŠÜđÿøĉöÖćøđÖĉé÷ĂéøüöđÞúĊę÷ÿĎÜÿčé 4.75 ÷ĂéêŠĂßĉĚîÿŠüî ĀúĆÜÖćøđóćąđúĊĚ÷Ü îćî 90 üĆî ĒúąđöČęĂđêĉöÙüćöđךöךî×ĂÜ BA ĔĀšÿĎÜ×ċĚîĂĆêøćÖćøđÖĉé÷ĂéøüöúéúÜ Ēúą Sheelavantmath et al. [15] ĕéš đóćąđúĊĚ÷ÜÖúšü÷ĕöš Geodorum densiflorum (Lam.) Schltr. ïîĂćĀćøÿĆÜđÙøćąĀŤÿĎêø MS đêĉöÿćøÙüïÙčöÖćøđÝøĉâđêĉïēê BA 5 ĕöēÙøēöúćøŤ ÿćöćøëßĆÖîĞćÖćøđÖĉé÷ĂéøüöĕéšÿĎÜÿčé 8.20 ÷ĂéêŠĂßĉĚîÿŠüî ĒêŠđöČęĂđóĉęöÙüćöđךöךî×ĂÜ BA ĔĀšÿĎÜ×ċĚîÝą

÷Ćï÷ĆĚÜÖćøđÖĉé÷ĂéĔĀöŠ ÝćÖÖćøýċÖþćóïüŠć BA öĊñúêŠĂÖćøĒïŠÜđàúúŤĒúąÖøąêčšîÖćøđÝøĉâđêĉïēêéšćîךćÜ×ĂÜóČß ĒêŠđöČęĂđóĉęö BA ĔĀšÿĎÜ×ċĚîÝąÿŠÜñú÷Ćï÷ĆĚÜÖćøóĆçîćÖćø×ĂÜ÷ĂéĒúąøćÖ

3.2 ÖćøßĆÖîĞćĔĀšđÖĉéøćÖ

ÖćøßĆÖîĞćĔĀšđÖĉéøćÖĔîÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ÝąđĀĘîĕéšüŠćđöČęĂđúĊĚ÷Üîćî 90 üĆî ÝąĔĀšðøĉöćèøćÖöćÖÖüŠćìĊę đúĊĚ÷Üîćî 60 üĆî ÙČĂ 10.4 øćÖ ēé÷ĂćĀćøìĊęßĆÖîĞćĔĀšđÖĉéøćÖĕéšöćÖìĊęÿčé ÙČĂĂćĀćøÿĎêø MS ìĊęđêĉö NAA 0.5 öĉúúĉÖøĆöêŠĂúĉêø ÿŠüîÖúšü÷ĕöšĀüć÷êąöĂ÷ìĊęđúĊĚ÷Üîćî 60 Ēúą 90 üĆî öĊðøĉöćèøćÖĕöŠĒêÖêŠćÜÖĆî éĆÜîĆĚîĂćĀćøìĊęđĀöćąÿĞćĀøĆïÖćøßĆÖîĞćĔĀšđÖĉé øćÖ ÙČĂĂćĀćøÿĎêø MS ìĊęđêĉö NAA 0.5 öĉúúĉÖøĆöêŠĂúĉêøìĊęđúĊĚ÷Üîćî 60 üĆî óïüŠćöĊÝĞćîüîøćÖöćÖìĊęÿčé ÙČĂ 4.0 øćÖ (êćøćÜ ìĊę 4.2.2)

êćøćÜìĊę 2 ÖćøßĆÖîĞćĔĀšđÖĉéøćÖđÞúĊę÷×ĂÜÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ĒúąĀüć÷êąöĂ÷ìĊęđúĊĚ÷Üîćî 30, 60 Ēúą 90 üĆî Ĕî ĂćĀćøÿĎêøêŠćÜ ė

ÿĎêøĂćĀćø đĀúČĂÜÝĆîìïĎø (øćÖ) Āüć÷êąöĂ÷ (øćÖ)

30 üĆî 60 üĆî 90 üĆî 30 üĆî 60 üĆî 90 üĆî

MS 1.7^cd 4.3^bc 7.5^b 1.6^bc 2.4^bc 2.8^bc

MS ìĊęđêĉö NAA 0.5 mg/L 1.8^cd 4.6^bc 10.4^a 2.0^b 4.0^a 4.5^a MS ìĊęđêĉö NAA 1.0 mg/L 1.9^c 5.2^ab 7.3^b 2.8^a 4.0^a 4.1^ab MS ìĊęđêĉö NAA 1.5 mg/L 2.7^b 5.2^ab 8.2^ab 1.6^bc 2.8^b 3.1^b

VW 2.0^bc 3.0^cd 4.5^c 1.2^d 1.6^c 1.8^c

VW ìĊęđêĉö NAA 0.5 mg/L 4.6^a 6.6^a 9.4^ab 1.5^cd 2.4^bc 2.9^bc VW ìĊęđêĉö NAA 1.0 mg/L 1.0^d 1.5^d 3.2^c 1.7^bc 2.7^b 3.0^bc VW ìĊęđêĉö NAA 1.5 mg/L 1.5^cd 2.0^d 2.7^c 1.4^cd 2.4^bc 3.2^b Āöć÷đĀêč: êĆüđú×ìĊęêćöéšü÷ĂĆÖþøìĊęđĀöČĂîÖĆî ĕöŠöĊÙüćöĒêÖêŠćÜÖĆîìćÜÿëĉêĉìĊęøąéĆïÙüćöđßČęĂöĆęî 95 đðĂøŤđàĘîêŤ (p<0.05) ēé÷üĉíĊ DMRT

ÝćÖñú×ĂÜÿĎêøĂćĀćøêŠĂÖćøđÖĉéøćÖ×ĂÜÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎøĒúąĀüć÷êąöĂ÷ ēé÷ÖćøđóćąđúĊĚ÷Ü đðøĊ÷ïđìĊ÷ïïîĂćĀćøÿĎêøÿĆÜđÙøćąĀŤ MS Ēúą VW óïüŠć Öúšü÷ĕöšĀüć÷ìĆĚÜ 2 ßîĉé đöČęĂđúĊĚ÷ÜĔîĂćĀćøÿĎêø MS ìĊęĕöŠđêĉö NAA ÿŠÜđÿøĉöÖćøđÖĉéøćÖđÞúĊę÷ĕéšÿĎÜÖüŠćĂćĀćøÿĎêø VW đöČęĂđúĊĚ÷Üîćî 90 üĆî àċęÜñúÖćøìéúĂÜìĊęĕéšĕöŠÿĂéÙúšĂÜÖĆïÜćîüĉÝĆ÷

×ĂÜ îć÷ĉÖć [9] ĒúąðøĆßóøøè [11] ĂćĀćøÿĎêø VW ÿŠÜđÿøĉöÖćøđÝøĉâđêĉïēê×ĂÜøćÖ ÝĞćîüîĒúąÙüćö÷ćü×ĂÜøćÖ ìĊę ÖúŠćüëċÜðøąē÷ßîŤ×ĂÜĒÙúđàĊ÷öĒúąĒöÖîĊđàĊ÷öÿĎÜ ÝċÜìĞćĔĀšÖćøóĆçîć×ĂÜøąïïøćÖđÖĉéĕéšéĊ ĒêŠóïüŠćöĊøć÷ÜćîÖćøýċÖþćÖćø ĔßšÿĎêøĂćĀćø MS Ĕî×ĆĚîêĂîÖćøßĆÖîĞćĔĀšđÖĉéøćÖ ÙČĂ ÜćîüĉÝĆ÷×ĂÜüĉßćâ [16] àċęÜøć÷ÜćîüŠćÖćøđúĊĚ÷ÜêšîĂŠĂî Dendrobium lamellatum Lindl. ïîĂćĀćøÿĎêø MS ìĊęđêĉö NAA 2.0 öĉúúĉÖøĆöêŠĂúĉêø öĊÖćøĒêÖøćÖéĊìĊęÿčé đîČęĂÜéšü÷ÿćøÖúčŠöĂĂÖàĉîđöČęĂ ĔßšĔîøąéĆïÙüćöđךöךîìĊęêęĞć ÝąöĊñúÖøąêčšîĔĀšđîČĚĂđ÷ČęĂóČßöĊÖćøĒïŠÜđàúúŤöćÖ×ċĚî ߊü÷ĔĀšöĊÖćøÿøšćÜøćÖĀøČĂ÷ĂéĔĀöŠ×ċĚîöć àċęÜ öĊÖćøîĞćöćĔßšĔîÖćøđóćąđúĊĚ÷ÜđîČĚĂđ÷ČęĂóČßĕéšÿĞćđøĘÝ

đöČęĂóĉÝćøèćñú×ĂÜ NAA ĒúąÿĎêøĂćĀćøÿĆÜđÙøćąĀŤìĊęöĊñúêŠĂÖćøđÖĉéøćÖ×ĂÜÖúšü÷ĕöšđĀúČĂÜÝĆîìïĎøĒúąĀüć÷

êąöĂ÷ ēé÷ÿĎêøĂćĀćø MS Ēúą VW ìĊęĕöŠđêĉöĒúąđêĉö NAA ìĊęøąéĆïÙüćöđךöךî 0.5, 1.0 Ēúą 1.5 öĉúúĉÖøĆöêŠĂúĉêø óïüŠć ÿĎêøĂćĀćø MS øŠüöÖĆï NAA 0.5 öĉúúĉÖøĆöêŠĂúĉêø ĔĀšÝĞćîüîøćÖöćÖìĊęÿčé 10.4 Ēúą 4.5 øćÖ êćöúĞćéĆïĀúĆÜðúĎÖđúĊĚ÷Ü 90 üĆî NAA đðŨîÿćøìĊęöĊùìíĉĝ×ĂÜĂĂÖàĉîÙŠĂîךćÜÿĎÜ đÙúČęĂî÷šć÷ĔîóČßĕéšđøĘü ĒêŠÿúć÷êĆüĕéšßšćĒúąđÖĉéÙüćöđðŨîóĉþêŠĂóČßĕéšÜŠć÷

ÝċÜöĊߊüÜÙüćöðúĂéõĆ÷ÙŠĂîךćÜĒÙï ĀćÖóČßĕéšøĆïĔîĂĆêøćìĊęöćÖđÖĉîĕðÝąìĞćĔĀšđÖĉéÙüćöđðŨîóĉþĒúąđÿĊ÷êŠĂÖćøđÖĉéøćÖĕéš ÝćÖÖćøìéúĂÜðøĉöćè NAA ìĊęöćÖÖüŠć 0.5 öĉúúĉÖøĆöêŠĂúĉêø×ċĚîĕð ÝċÜöĊñúìĞćĔĀšöĊðøĉöćèøćÖîšĂ÷

3.3 Öćø÷šć÷êšîĂŠĂîĂîčïćúĔîēøÜđøČĂî

îĞćêšîĂŠĂî×ĂÜÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎøĒúąĀüć÷êąöĂ÷ ìĊęĕéšÝćÖÖćø×÷ć÷óĆîíčŤĔîÿõćóðúĂéđßČĚĂĂĂÖðúĎÖ ĔîđéČĂîöĉëčîć÷î 2564 ÝĞćîüî 50 Ēúą 40 êšî êćöúĞćéĆï úšćÜéšü÷îĚĞćÿąĂćé ĒߊîĚĞć÷ćÖĆîøćđðŨîđüúć 15 îćìĊ ĔßšÖćï öąóøšćüßîĉéĕöŠêĉéđðúČĂÖĒ×ĘÜéšćîîĂÖ ĒúąÖćïöąóøšćüßîĉéĕöŠêĉéđðúČĂÖĒ×ĘÜéšćîîĂÖøŠüöÖĆïēôöĀŠĂñúĕöšđðŨîüĆÿéčĀŠĂøćÖ üćÜĔîÝčéìĊęöĊĂćÖćýëŠć÷đìéĊ ĒêŠĕöŠöĊúöĒøÜ ĒúąĕöŠēéîòî óïüŠćĀúĆÜĂĂÖðúĎÖĂîčïćú 4 ÿĆðéćĀŤ (đéČĂîÖøÖãćÙö) êšîĂŠĂî Öúšü÷ĕöšĀüć÷ìĆĚÜ 2 ßîĉé ÷ĆÜĕöŠóïÖćøêć÷ ĒêŠđöČęĂđúĊĚ÷ÜêŠĂÝîëċÜ 8 ÿĆðéćĀŤ (đéČĂîÿĉÜĀćÙö) Öúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ìĊę ĔßšüĆÿéčĀŠĂøćÖđðŨîÖćïöąóøšćüßîĉéđéĊ÷ü öĊêšîøĂéßĊüĉê 3 êšî ÙĉéđðŨî 6 đðĂøŤđàĘîêŤ ÿŠüîÖúšü÷ĕöšĀüć÷êąöĂ÷ êšîĂŠĂîêć÷

ìĆĚÜĀöéïîüĆÿéčìĆĚÜ 2 ßîĉé ĒúąĕéšĂĂÖðúĎÖÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎøĂĊÖÙøĆĚÜĔîߊüÜđéČĂîÖøÖãćÙö ÝĞćîüî 50 êšî ēé÷Ĕßš ÖćïöąóøšćüøŠüöÖĆïēôöĀŠĂñúĕöšđðŨîüĆÿéčĀŠĂøćÖ ĔĀšñúĔîìĉýìćÜđéĊ÷üÖĆïÖćøĂĂÖðúĎÖĔîđéČĂîöĉëčîć÷î ĒúąđöČęĂđúĊĚ÷Üîćî 8 ÿĆðéćĀŤ (đéČĂîÖĆî÷ć÷î) óïêšîøĂéßĊüĉê 12 êšî ÙĉéđðŨî 24 đðĂøŤđàĘîêŤ

õćóìĊę 1 êšîĂŠĂîđĀúČĂÜÝĆîìïĎøĀúĆÜĂĂÖðúĎÖĔîÿõćóēøÜđøČĂî

4. ÿøčðñúÖćøìéúĂÜĒúą×šĂđÿîĂĒîą

ĂćĀćøìĊęđĀöćąÿöĔîÖćøßĆÖîĞćĔĀšđÖĉéêšîĒúąÖćøđóĉęöðøĉöćè×ĂÜÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ÙČĂĂćĀćøÿĎêø MS ìĊę đêĉö BA 5 öĉúúĉÖøĆöêŠĂúĉêøđöČęĂđúĊĚ÷Üîćî 60 üĆî ÿŠüîÖúšü÷ĕöšĀüć÷êąöĂ÷ ÙČĂĂćĀćøÿĎêø VW ìĊęðøćýÝćÖÿćøÙüïÙčöÖćø đÝøĉâđêĉïēê BA đöČęĂđúĊĚ÷Üîćî 90 üĆî

ĂćĀćøìĊęđĀöćąÿöĔîÖćøßĆÖîĞćĔĀšđÖĉéøćÖ×ĂÜÖúšü÷ĕöšĀüć÷đĀúČĂÜÝĆîìïĎø ĒúąĀüć÷êąöĂ÷ ÙČĂĂćĀćøÿĎêø MS ìĊę đêĉö NAA 0.5 öĉúúĉÖøĆöêŠĂúĉêøđöČęĂđúĊĚ÷Üîćî 90 Ēúą 60 üĆî êćöúĞćéĆï

ÿĞćĀøĆïÖćøĂĂÖðúĎÖêšîĂŠĂî×ĂÜđĀúČĂÜÝĆîìïĎø ÙüøĂĂÖðúĎÖĔîߊüÜìĊęöĊÙüćößČĚîÿĆöóĆìíŤÿĎÜ (ÖøÖãćÙö - ÖĆî÷ć÷î) đóČęĂĔĀšöĊÖćøðøĆïêĆüÖĆïÿõćóõć÷îĂÖĕéšÖŠĂîđךćÿϊߊüÜĒúšÜ ÿŠüîĀüć÷êąöĂ÷ êšĂÜìĞćÖćøýċÖþćüĉíĊÖćøĂĂÖðúĎÖĔĀöŠ đóČęĂĀćüĆÿéč üĉíĊ ĒúąßŠüÜđüúćìĊęđĀöćąÿöêŠĂĕð

5. đĂÖÿćøĂšćÜĂĉÜ

[1] Yang, L., Wang, Z.,and Xu, L. (2006). Simultaneous determination of phenols (bibenzyl, phenanthrene, and fluorenone) in Dendrobium species by high-performance liquid chromatography with diode array detection. Journal of Chromatography A. 1104, 230-237.

[2] Peyachoknagul S, Mongkolsiriwatana C, Wannapinpong S, Huehne PS, Srikulnath K. Identification of native Dendrobium species in Thailand by PCR-RFLP of rDNA-ITS and chloroplast DNA. ScienceAsia.

2014; 40 (2) : 113-20.

[3] Liu, Y. N., Pan, S. L., Peng, C. Y., Huang, D. Y., Guh, J. H., Chen, C. C., Shen, C. C. and Teng, C. M. (2010).

Moscatilin repressed lipopolysaccharide induced HIF-1ɲ accumulation and NF-KB activation in murine RAW264.7 cells. Shock, 33 (1), 70-75.

[4] Kowitdamrong, A., Chanvorachote, P., Sritularak, B., and Pongrakhananon, V. (2013). Moscatilin inhibits lung cancer cell motility and invasion via suppression of endogenous reactive oxygen species. BioMed Research International, 2013, Article ID 765894

[5] Tsai, A.C., Pan, S.L., Liao, C.H., Guh, J.H., Wang, S.W., Sun, H.L.,et al. (2010). Moscatilin, a bibenzyl derivative from the India orchid Dendrobriumloddigesii, suppresses tumor angiogenesis and growth in vitro and in vivo. Cancer Letter, 292(2), 163-170.

[6] Seidenfaden G. (1985) Orchid Genera in Thailand XII. Dendrobium Sw. Opera Botanica no. 83. Council for Nordic Publications in Botany, Copenhagen.

[7] ÷čóĉî ÖþĉîđÖþöóÜþŤ ÞĆêêŤîõć ׊öĂćüčí ĒúąĂĆöóĉÖć ðčîîÝĉê. (2560). øć÷ÜćîñúÜćîüĉÝĆ÷đøČęĂÜđêĘöÿĉĚîÿčéðŘÜïðøąöćè 2559 đøČęĂÜ ÖćøìéúĂÜøüïøüöĒúąÙĆéđúČĂÖÖúšü÷ĕöšÿÖčú Dendrobium ìĊęöĊýĆÖ÷õćóđðŨîÿöčîĕóø ēÙøÜÖćøüĉÝĆ÷ĒúąóĆçîćÖúšü÷ĕöš Dendrobium ìĊęöĊýĆÖ÷õćóđðŨîÿöčîĕóø. ÖøčÜđìóĄ: ÿëćïĆîüĉÝĆ÷óČßÿüî ÖøöüĉßćÖćøđÖþêø.

[8] ĂïÞĆîìŤ ĕì÷ìĂÜ. (2549). Öúšü÷ĕöšđöČĂÜĕì÷. óĉöóŤÙøĆĚÜìĊę 12. ÖøčÜđìóĄ. ÿĞćîĆÖóĉöóŤïšćîĒúąÿüî.

[9] îć÷ĉÖć ÿĆîìćøčîĆ÷. (2558). ÖćøýċÖþćÿĎêøĂćĀćøìĊęöĊñúêŠĂÖćøđÝøĉâĒúąÖćøĂĂÖéĂÖ×ĂÜÖúšü÷ĕöšđĀúČĂÜÝĆîìïĎø (Dendrobium friedericksianum Rchb. f.) ĔîĀúĂéìéúĂÜ. ÖćøðøąßčöüĉßćÖćøĒúąđÿîĂñúÜćîüĉÝĆ÷

øąéĆïßćêĉ “ÿøšćÜÿøøÙŤĒúąóĆçîć đóČęĂÖšćüĀîšćÿĎŠðøąßćÙöĂćđàĊ÷î” ÙøĆĚÜìĊę 2 18-19 öĉëčîć÷î 2558 è üĉì÷ćúĆ÷

îÙøøćßÿĊöć ĂĞćđõĂđöČĂÜ ÝĆÜĀüĆéîÙøøćßÿĊöć, 155-162.

[10] Stewart S.L. & Kane M.E. (2006). Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tissue Org.

Cult, 86, 147-158.

[11] ðøĆßóøøè ĀîĎÝĊî. (2550). ðŦÝÝĆ÷ìĊęöĊñúÖćøđÝøĉâĒúąÖćøĂĂÖéĂÖ×ĂÜÖúšü÷ĕöšđĀúČĂÜÝĆîìïĎø. üĉì÷ćîĉóîíŤ×ĂÜÖćøĂĂÖ éĂÖ×ĂÜÖúšü÷ĕöšđĀúČĂÜÝĆîìïĎø. ÿć×ćüĉßćóČßýćÿêøŤïĆèæĉêüĉì÷ćúĆ÷ öĀćüĉì÷ćúĆ÷ÿÜ×úćîÙøĉîìøŤ.

[12] ÿčõćüéĊ øćöÿĎêø ðøĊéć ïčâđüýîŤ Ēúąüøĉ÷ć îüúîčß. (2558). ñú×ĂÜÿĎêøĂćĀćøêŠĂÖćøđÝøĉâđêĉïēê×ĂÜÖúšü÷ĕöšđĂČĚĂÜÖčĀúćï ÖøąđðŞćðŗéĔîĀúĂéìéúĂÜ. üćøÿćøóČßýćÿêøŤÿÜ×úćîÙøĉîìøŤ, 2 (4), 11-14.

[13] Ăćø÷ć ĂćÝđÝøĉâ đìĊ÷îĀĂö íĊøõøèŤ êčšöîšĂ÷ ðøĆßâć đêüĉ÷ą Ēúąüĉì÷ć ĒÖšüýøĊ. (2558). Öćø×÷ć÷óĆîíčŤđĂČĚĂÜßšćÜîšćüéšü÷

ÖćøđóćąđúĊĚ÷ÜđîČĚĂđ÷ČęĂ. üćøÿćøüĉì÷ćýćÿêøŤđÖþêø, 46 (3) (óĉđýþ), 101-104.

[14] îčßÝøĊ ÿĉÜĀŤóĆîíŤ ĒúąĒóøüóøøè ÝĆîđÜĉî. (2563). ñú×ĂÜÿĎêøĂćĀćøĒúąÿćøÙüïÙčöÖćøđÝøĉâđêĉïēê×ĂÜóČßêŠĂÖćø đÝøĉâđêĉïēê×ĂÜđĂČĚĂÜßšćÜîšćüĔîÿõćóðúĂéđßČĚĂ. üćøÿćøđÖþêøóøąÝĂöđÖúšć, 38 (3), 288-295.

[15] SheelavantmathS.S., Murthy H.N., Pyati A.N., Ashok Kumar H.G. and Ravishankar B.V. (2000) In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture. Plant Cell, Tissue and Organ Cult. 60 (2), 151-154.

[16] üĉßćâ ĒðÜđöČĂÜ ĒúąĂîčóĆîíŤ ÖÜïĆÜđÖĉé. (2555). ñú×ĂÜÿćøÙüïÙčöÖćøđÝøĉâđêĉïēêêŠĂÖćøóĆçîć×ĂÜêšîĂŠĂî Öúšü÷ĕöšĀüć÷ĒïîĔîÿõćóðúĂéđßČĚĂ, Ĕîøć÷ÜćîÖćøðøąßčöüĉßćÖćø óąđ÷ćüĉÝĆ÷ÙøĆĚÜìĊę 1 : ÖúčŠöÖćøđÖþêø üĉÝĆ÷, öĀćüĉì÷ćúĆ÷óąđ÷ć, óąđ÷ć, 7 î.