Identification And Analysis Of Defoliation Responsive Genes In Alfalfa. (6412)
Authors:
K.A.L. Al-Hadid* - Purdue
S.M. Cunningham - Purdue University J.J. Volenec - Purdue University
Abstract:
Shoot growth of alfalfa originates from buds present on crowns near the soil surface, but we do not understand the physiological and molecular responses that occur as quiescent buds begin growth after shoot removal. Our objective was to determine defoliation-induced changes in gene expression that occur in crown buds of alfalfa. Four field replicates of a semi-dormant cultivar (Pioneer 5454) were established in May of 2000, and crown buds sampled 14 h and 38 h after defoliation in summer 2001. In order to study changes in gene expression in developing buds in autumn and the effects of fall dormancy we sampled buds 15 and 63 h
post-defoliation from the cultivars Vernal (fall dormant) and CUF 101 (fall non-dormant). Differential display was used to identify defoliation-induced or defoliation-repressed transcripts. Dot blotting and Northern blotting are being used to confirm the result obtained from differential display. Full-length cDNAs of the confirmed transcript will be isolated and sequenced. Preliminary results indicate that our defoliation-sensitive clones share sequence similarity with transcripts involved in cell cycle regulation such as histone H4 and histone H2B.
Speaker Information: Khaldoun Al-Hadid, Purdue, 1901 Union St./ Apt# 340, Lafayette, IN 47904; Phone: 765-447-3819; E-mail: [email protected]
Session Information: Tuesday, November 2, 2004, 1:00 PM-3:00 PM Presentation Start: 1:00 PM (Poster Board Number: 1241)
Keywords: Alfalfa; Fall dormancy; Bud deveopment; Gene Expression
