Molecular Mapping of the Tenacious Glumes, Tg, Gene in Common Wheat. (W-nalam115954-oral)
Authors:
V. Nalam* - Oregon State University O. Riera-Lizarazu - Oregon State University C.J.W. Watson - Oregon State University M.I. Vales - Oregon State University
Abstract:
Molecular Mapping of the Tenacious Glumes, Tg, Gene in Common Wheat. Nalam, V.J., C.J.W.
Watson, M.I. Vales, and O. Riera-Lizarazu Synthetic hexaploids, derived from hybridizations between tetraploid wheat (Triticum turgidum) and Aegilops tauschii, are non-free-threshing. Glume tenacity and the non-free-threshing characteristic in synthetics, carrying Q or q, are due to the action of tenacious glumes, Tg, a semi-dominant gene on the short arm of chromosome 2D. Current evidence suggests that at least two mutations, q to Q and Tg to tg, were necessary to generate free-threshing hexaploid wheats with the QQtgtg allelic constitution. Since Tg has played an important role in the evolution of bread wheat, we have initiated a project to isolate and characterize this gene. In a previous study, we localized Tg to a ~20-cM region flanked by two RFLP markers (Xbcd102 and Xcdo1379). In this study, we mapped 11 SSR markers and localized Tg to a smaller region (~4 cM) flanked by Xgwm296 and Xwmc25. We are currently using a combination of genomics resources and bulked segregant analysis to increase marker saturation, and developing a population to construct a high-resolution map of the Tg region.
Speaker Information: Vamsi Nalam, Oregon State University, Dept. Crop and Soil Science Oregon State Universi, Corvallis, OR 97331; Phone: 541-737-5839; E-mail: [email protected]
