View of Porphyromonas gingivalis FimA Type II - PVXCP Fusion DNA Vaccine Expression in Mammalian Cells

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ARSQ@BS

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@M@KXYDCAX6DRSDQM!KNS@M@KXRHRTRHMF@MSH/57"/@MSHANCX

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Keywords :P.gingivalisU@BBHMD/DQHNCNMSHSHRU@BBHMD#- U@BBHMD/QNSDHMDWOQDRRHNMHML@LL@KH@M BDKKR

*Lecturer, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand.

Porphyromonas gingivalis%HL 3XOD((/57"/%TRHNM

#- 5@BBHMD$WOQDRRHNMHM,@LL@KH@M"DKKR

**)@MSHO@)NARQH 6@MUHR@/HKTDJ /NMI@QTR/DRNNMFMDQM *TRTL@)@LCDD**

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Porphyromonas gingivalis (P. gingivalis)

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%NQ 6DRSDQM AKNS @M@KXRHR BDKKR VDQD KXRDCVHSG^N"OQDGD@SDCOQNSDHMR@LOKDKN@CHMF ATEEDQ/QNSDHMRHMBDKKBTKSTQDLDCHTL@MCBDKK KXR@SDVDQDRDO@Q@SDCNM2#2/ &$@MC VDQD RTAIDBSDC SN 6DRSDQMAKNS @M@KXRHR TRHMF Q@AAHS@MSH/57"/@MSHRDQTLENKKNVDCVHSG'1/

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Figure 1. Electrophoresis of Leader-FimA PCR product (1.3 kb).

Number 1 – 19 indicate clone number. M: VC 100 bp Plus DNA ladder.

Figure 2. Electrophoresis of Leader-FimAPVXCP PCR product (2 kb).

Numbers indicate clone number. M: VC 100 bp Plus DNA ladder.

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"KNMDMTLADQV@RRDKDBSDCENQOK@RLHC

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&(

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!"+RBEU/57"/V@R@ONRHSHUDBNMSQNKENQOQNSDHM DWOQDRRHNM '$* BDKKR VHSG MN SQ@MREDBSHNM VDQDTRDC@R@MDF@SHUDBNMSQNK%HL /57"/

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OQNSDHMHMRHCDSGDBDKKRLHFQ@SDCVHSG@OO@QDMS LNKDBTK@Q VDHFGS NE J#@ VGHKD SGD OQNSDHM RDBQDSDCHMSNSGDLDCHTLLHFQ@SDCVHSG@OO@QDMS LNKDBTK@QVDHFGSNEJ#@%HF(MOB#-

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%HL /57"/BNTKCADDWOQDRRDCHML@LL@KH@M BDKKR

Figure 3. Electrophoresis of FimA-PVXCPRT-PCR product (1.9 kb) of RNA extracted from HEK293 cells transfected with pcDNA3.Fim-PVXCP (293-F) or pcDNA3.BCL1scfv-PVXCP (293-B)

or no transfection (293). No RT indicates RT PCR without reverse transcriptase.

M:VC 100 bp Plus DNA ladder

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#HRBTRRHNM

3GD BNMRSQTBSDC OB#- %HL /57"/

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@LHMN@BHCRDPTDMBDNE%HL /57"/V@R@M@KXYDC TRHMF-DS-&KXBRDQUDQ:<@MC-DS.&KXB RDQUDQ:<SGDC@S@RGNVDCSG@S%HL /57"/

BNMS@HMRL@MXONSDMSH@[email protected]@SHNM RHSDR %HL /57"/ LHFGS AD FKXBNRXK@SDC @MC SGDQDENQDSGDRHYDNESGDRDBQDSDCOQNSDHMV@R K@QFDQSG@MSGDOQNSDHMQDL@HMHMRHCDSGDBDKKR /QNSDHMDWOQDRRDCEQNLSGHR#- U@BBHMD HMBKTCDC ETKKKDMFSG %HL SXOD(( VGHBG L@X BNMS@HMRL@MX@MSHFDMHBCDSDQLHM@MSR(SG@R

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%HL SXODR

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#- U@BBHMDSNHMCTBDOQNSDBSHUDHLLTMHSXSGD U@BBHMDVHKKADETQSGDQ@CLHMHRSDQDCHMSN@MHL@K LNCDKR:<#- U@BBHMDRTRHMF/57"/ETRHNM HMOB#- A@BJANMDG@UDADDMRGNVMSNHMCTBD GHFG KDUDKR NE @MSHANCX @MC OQNSDBSHNM @F@HMRS RODBHEHB@MSHFDM:<3GDQDENQDSGHR%HL /57"/

#- U@BBHMDHRDWODBSDCSNHMCTBDGHFG@MSHANCX KDUDK@RVDKK

Figure 4. Detection of FimA-PVXCP protein by Western blot analysis.Proteins remained inside the cells (cell) and in the medium (s/n) of HEK293 cellstransfected with pcDNA3.Fim-PVXCP

(293-F) or pcDNA3.BCL1scfv-PVXCP (293-B) or no transfection (293) were analyzed. M:

Chromatein Prestained Protein Ladder, vivantis.

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