Curriculum vitae
HANI ZAKARIA ASFOUR
Department of Medical Biology Faculty of Medicine & Allied Science
King Abdulaziz University
Jeddah, Saudi Arabia
HANI ZAKARIA ASFOUR
Department of Medical Biology Faculty of Medicine
King Abdulaziz university Jeddah, Saudi Arabia
PERSONAL
Marital status: Married Nationality: Saudi Date of birth: 11/5/ 1974
Place of Birth: Jeddah, Saudi Arabia
Languages: Arabic & English
Home Address: Jeddah, Saudi Arabia
Work Address: P.O Box 80205 Jeddah 21589, Saudi Arabia.
(Tel) Office : 02-6400000 (Ext. 22122, 22082), Home :02-6919182 Mobile# 0506255504
Academic Qualifications
2006 - Ph.D. Molecular Biology, College of Art and Sciences, University of North Texas, Denton, Texas, U.S.A.
2002 - M.S. Molecular Biology (Microbial Genetics), College of Art and Sciences, University of North Texas, Denton, Texas, USA.
1997 - B.Sc. Microbiology,(Honor) College of Science, King Abdulaziz University, Saudi Arabia
Work Experience - 1998- Present
Present
Assistant Professor, Chairman of Medical Biology Department, School of Medicine, King Abdulaziz University, Jeddah, SaudiArabia.
2003-2006
Teaching assistant, Biology Department, University of North Texas, U.S.A
1998-1999
Teaching Assistant , Medical Biology Dept, School of Medicine, King Abdulaziz University
When I was Teaching assistant at King Abdulaziz university Faculty of Medicine, Department of Medical Biology, I was teaching and supervising tutorials of small group teaching, and laboratory classes for specialization for first year Medical students, Dentistry, Pharmacy, and Medical Technology students.
I was also responsible for teaching several undergraduate students the principle of microbiology at university of North Texas, Biology Department.
Publications
1. Asfour, H. and A.E. Hadhoud. (2007). Metallo beta-lactamase production in Pseudomonas and sensitivity to antibiotics in Intensive Care Units Patients of King Abdulaziz Hospital & Oncology Center, Jeddah, KSA. (In preparation).
2. Asfour, H., M.H. Al-Qahtani and S.M. Al- Assouli. (2007). The prognostic value of Heat Shock protein in beast cancer. (In preparation).
3. Asfour, A. (2006). Regulation of pyrimidine biosynthesis and virulence factor production in wild type, Pyr- and Crc- mutants in Pseudomonas aeruginosa. Ph.D.
Dissertation. University of North Texas. U.S.A
4. Asfour, A. (2002). Effector response of the aspartate transcarbamoylase (ATCase) from wild type Pseudomonas putida and a mutant with 11 amino acids deleted at the N-terminus of PyrB. M.S. Thesis. University of North Texas. U.S.A
Additional Professional Activities
During the past years my research emphasis was placed on:
Pyrimidine metabolism in bacteria with special focus on the regulatory enzyme, aspartate transcarbamolase (ATCase).
All pyrimidine enzymes assays, using different techniques such as Spectrophotometer.
The role of pyrimidine enzymes in the regulation of the pyrimidine pathway and other chemical pathways.
Genetic organization and biochemistry of prokaryotic DNA.
Practical application of Polymerase Chain Reaction (PCR)
Through these research projects I obtained knowledge and techniques in recombinant DNA technology, amplifying of cloned gene, bacterial strain construction, Making competent cells, transformation, selection and purification of wild type and mutant ATCase by using affinity chromatography. .
The focus of my master and Ph.D research was on pyrimidine metabolism in bacteria with special emphasis on the regulatory enzyme. In my master thesis my research was focused in the study of the regulatory enzyme Aspartate transcarbamoylase (ATCase) in Pseudomonas putida. In this project I dealt with the purification and kinetic characterization of mutant and wild type Pseudomonas putida ATCase. During that time I experienced different techniques such as, strain construction, making competent cells, transformation, selection, and purification of ATCase by using ionic exchange column.
In addition to that, techniques in cell biology were also experienced by taking a practical course.
The main focus in my Ph.D research was to study the relation between different mutants in the pyrimdine genes and the production of virulence factors in the opportunistic pathogen, Pseudomonas aeruginosa. This study showed that all mutant strains were deficient in the production of virulence factors. Low level production of pyocyanin, hemolysin, casein protease, and elastase were found in the mutant strains compared to the wild type strain.
Swimming, swarming, and twitching motility were also impaired. Many different techniques were experienced during that time including the following: polymerase chain reaction (PCR) and its different application, hybridization, Southern blot, gel electrophoresis, DNA isolation, Protein purification, and construction of mutant strain.
Workshops and Conferences Attended
Training course for INNOVATION MANAGEMENT during the 5th Quality Day Program at King Abdulaziz University, April 29, 2008
Workshop of ‘Effective Assessment of student performance’ 8th March to 13th March 2008. at King Fahd Medical Research Center, King Abdulaziz University, Jeddah. KSA.
Study Guides: Their Uses and Preparation ( 22 April 2007, King Abdulaziz University, school of medicine
Conference about an introduction to center of Excellence in Genomic Medicine Research (CEGMR) At king Fahad Research Center, King Abdulaziz University on 2nd June 2007.
Communication Skill ( 23June- 4July 2007, King Abdulaziz University)
Workshop of Essential Skills for Medical Education Course, 3-4 Sep 2007, King Abdulaziz University, School of Medicine.
6thGCC Medical Education conference held at King Abdulaziz University, Jeddah, KSA 25-26 Shawal 1428/ 6-7 November, 2007
Professional Societies’ Membership
1. Active Member of American Society of Microbiology 2. Member of Saudi biological Society (SBS)
3. Symposia Conferences and workshops
4. Attending a number of pertinent conferences dealing with my specialization
