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Lab # 6 - The Effects of Temperature on Growth

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The Effects of Temperature on Growth

Physiology of microbiology KING ABDULAZIZ UNIVERSITY

Lab # 6

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Environmental Effects on Microbial Growth:

Bio 336

The Effects of Temperature on Growth

1-Physical factors

2- Chemical factors

3- Biological factors

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The Physical factors :

• Temperature

• Radiation

• Oxygen classes

• pH

• Osmotic pressure

The Effects of Temperature on Growth

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Bio 336

The Effects of Temperature on Growth

Dependence of growth of microorganisms on temperature is largely related to activation and inactivation of enzyme systems in

microorganisms.

Temperature and Microbial Growth:

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The Effects of Temperature on Growth

The effects of temperature contribute to the death of microbial cells

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Bio 336

The Effects of Temperature on Growth

The range of temperatures for the growth of a given microbial species can be expressed as three cardinal temperatures so Each microbes has 3 different temperature range:

Minimum temperature: it’s the lowest temperature the microbe can grow on

Maximum temperature: the highest Optimum temperature: the best

Psychrophilic: low temperature <10 oC Mesophilic: 20- 40 oC

Thermophilic: high temperature > 60 oC

Temperature Range:

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The Effects of Temperature on Growth

Classification of Microorganisms by Temperature Requirements

Temperature is the major environmental factor

controlling microbial growth

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The Effects of Temperature on Growth

Experiment # 1

estimation of microbial growth optimal temperatures q Material and Experimental Protocols:

q Observation and results:

q Conclusion:

5 C

10 C

28 C

37 C

42

C○ 55

C

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Bio 336

The Effects of Temperature on Growth

Thermal Death Time TDT of microorganisms :

Microbes can be partially or completely eliminated by heat treatment.

However, the time and temperature used are dependent on the type and number of microorganisms.

Thermal death point: it is the temperature at which the microbe dies due to the high temperature.

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The Effects of Temperature on Growth

Experiment # 2

Thermal Death Point Measurement.

q Material and Experimental Protocols:

10 minuets then put all tubes in ice bath.

55

control

45 60 80

100

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