Saudi Journal of Biological Sciences

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Original article

Ethnobotanical, phytochemical and antimicrobial activity of Halexylon salicornicum (Ramth) as a graze and promising shrub against selected animal microbes

Rehab M.A. El-Desoukey

^a,c,

⇑ , Fawziah M. Albarakaty

^b

, Nurah M. Alzamel

^c,

⇑ , Mashail N. AlZain

^d

aMicrobiology and Immunology Department, National Research Centre, Giza, Egypt

bDepartment of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Al Mukarramah, P.O. Box: 715, Saudi Arabia

cBiology Department, Faculty of Science and Humanities in Al Quwai’iyah, Shaqraa University, Shaqraa 11961, Saudi Arabia

dDepartment of Biology, College of Sciences, Princess Nourah bint Abdulrahman University, Riyadh 11451, Saudi Arabia

a r t i c l e i n f o

Article history:

Received 2 February 2022 Revised 22 April 2022 Accepted 23 May 2022 Available online 29 May 2022

Keywords:

Natural antimicrobial Halexylon salicornicum Ramth

Grazing herbs Shrub

Animal pathogen Camel Ethnobatanical

a b s t r a c t

Folk medicine, including plants, has been utilized for humans and animals since the dawn of civilization.

Because of the widespread problem of antimicrobial resistance around the world, one of the mainly significant challenges in microbiological research is to achieve a replacement antibiotic with the fewest adverse effects. Camel and ruminant grazing were provided by the wild shrubHalexylon salicornicum (Ramth). However, despite prior research demonstrating its antimicrobial action against human diseases, no investigations on its antimicrobial activity against animal pathogens have been conducted to far. The goal of this study is to investigate whetherHalexylon salicornicumaqueous and solvent extracts have antimicrobial activity on a variety of animal pathogens isolated from cattle and poultry using the agar well diffusion method (Enterococcus faecium, Shigella flexneri, Bacillus Cereus, Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, Streptococcus pyogens, Pseudomonas aerogenes, Salmonella typhimurium, and Candida albicans) moreover it’s ethnobotanical and phytochemical. All of the extracts tested had antimicrobial efficacy against tested strains and included bioactive chemicals, particularly the acetone extract, had the highest antibacterial activity. As a result, it can be stated thatHalexylon salicornicum is a promising important shrub that might be used as a natural antimicrobial alternative for animals or as a food additive.

!2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

1. Introduction

Half of the deaths in animals by infectious diseases are due to microbes especially that lead to diarrhea the leading cause of ill- ness and mortality in poor nations. Most of these cases due to (Sal- monella and E.coli) (Alavijeh et al., 2012, El-Desoukey, 2015, 2017).

In neonates are commonly associated with (C. parvum, E. coli, Rota and Coronavirus) which can lead to outbreaks (Merck Sharp and

Dohme Corp, 2014). In addition to Candida albicans is the main cause of systemic fungal infections (Sofos et al., 1998).In dairy cows the most common cause of clinical mastitis is mainly Staphy- lococci and Klebsiella pneumonia (Thorberg et al., 2009, Schuh and Weinstock, 1985). Bacillus cereus is the main cause of necrotizing placentitis (Munoz et al., 2006). Due to the high cost of chemical antibiotics, long degradation period, it’s side effects moreover the increase of antimicrobial resistance, especially in animal products, therefore a lot of researchers trying to find an alternative natural antimicrobial from plants and herbs (Abd El-Latif et al., 2002, Buchanan et al., 2008, Duraipandiyan et al., 2006). The chemical composition of the plant plays an important role as an alternative medicinal therapy, especially in it’s bioactive compound such as alkaloids, flavonoids tannins and saponins (Kanife and Odesanmi, 2012, Tariq et al., 2018). Also, a lot of people in developing countries believe in and use the herbs in folk medicine for both man and animals which do not require prolonged or expense treatment (Aziz et al., 2018).

https://doi.org/10.1016/j.sjbs.2022.103328

1319-562X/!2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

⇑Corresponding authors.

E-mail addresses:[email protected](R.M.A. El-Desoukey),fmbara- [email protected] (F.M. Albarakaty), [email protected] (N.M. Alzamel), mnal- [email protected](M.N. AlZain).

Peer review under responsibility of King Saud University.

Production and hosting by Elsevier

Contents lists available atScienceDirect

Saudi Journal of Biological Sciences

j o u r n a l h o m e p a g e : w w w . s c i e n c e d i r e c t . c o m

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Haloxylon salicornicum is a shrub or undershrub belonging to the Chenopodiaceae family that is present in many countries in Asia and Africa including the Kingdom of Saudi Arabia (El- Wahab et al., 2014, Phondani et al., 2015). Haloxylon salicornicum can be physiologically and ecologically adapt and tolerate the water limitation, salty land and high temperature, each part of the shrub is traditionally used for animal food (fruiting top, seeds), human food during drought especially in India and shrub wood as fuel (Ajabnoor et al., 1984). H. salicornicum has been approved to have anti-inflammatory, anti-diabetic and Antiseptic activities (Awaad et al., 2014). These all activities in addition to hepatoprotective are supposed to be due to the shrub’s secondary metabolites as triterpenoids, alkaloids, tannins, glycosides, flavonoids and tannins (Eman, 2011, Karimi et al., 2011). The organic extracts of the shrub showed significant antibacterial against some bacteria of human origin (Salmonella typhi, Staphylococcus aureus, Micrococcus luteus, Sar- cina ventriculi and Bacillus subtilis) in addition to antifungal effect against some fungi of human origin (Penicillium chryso- genum, Aspergillus flavus, Candida albicans, Aspergillus fumigates and Candida tropicalis) (Dash et al., 2008, Kim and Van Ta, 2011).These antimicrobial activities due to its phytochemical content (tannins, alkaloids, saponins and flavonoids) have a lot of useful pharmacological effects (Hammer et al., 2001, Shankar and Kumar, 1984). However H. salicornicum is not used globally (Ajabnoor et al., 1984). It has been used as important fodder for camels, sheep and goats in some countries including Kingdom of Saudi Arabia (Singh et al., 2015). Also it has been used in folk medicine for some diseases of man and animals (Arshad et al., 2002).

To far, however, there have been no studies that have investigated the antimicrobial activity of H. salicornicum extract against pathogens from animal sources especially as a grazing herb that is consumed by camels and small ruminants select it for eating by instinct that my resume the use due to the Zoopharmacognosy as animals medicate themselves by selecting natural substances (plants, herbs, clay and insects) to reduce the risky effect of pathogens (Kapadia et al., 2014, Attardo and Sartori, 2003). Especially as the safety for therapeutic uses of aqueous extract of the shrub H. salicornicum has been demonstrated and can be used without any significant toxicity (Ullah et al., 2019). So this research aimed to inspect the phytochemical, ethnobotanical and antimicrobial effect of H. salicornicum against pathogens of animal origin to give more attention to this neglected shrub.

2. Materials and methods

2.1. Materials and reagents

2.1.1.

Reagents used for extraction (Ether, Ethanol and Acetone) and reagents used for phytochemical tests (Ferric Chloride, Glacial Acetic Acid, Alcoholic Potassium Hydroxide, Sulfuric Acid, chloroform, ammonia and Formaldehyde) were purchased from Arkan Group, Fisher chemical

^"

in addition to media and antimicrobial used for antimicrobial assay (Nutrient Agar, Potato Dextrose Agar, Mueller Hinton Agar, Peptone Water, Mc Farland BSS 0.5, Cipro- floxacin, tetracycline, cefpodoxime, Erythromycin, Gentamycin, Augmentin and Nystatin) were also purchased from Arkan Group, scharlab

^"

.

2.1.1. Collection of shrub

H. salicornicum aerial parts were collected during the flowering season in March 2018 from Valley of the Quwai’, in Al Quwai’iyah, a large Province beside Riyadh Province, Saudi Arabia.

2.1.2. Bacterial and fungal strains

The bacterial and fungal strains used in this study (Enterococcus faecium, Shigella flexneri, Bacillus Cereus, Klebsiella pneumoniae, Sta- phylococcus aureus, Escherichia coli, Streptococcus pyogens, Pseu- domonas aerogenes, Salmonella typhimurium, and Candida albicans) were isolated from some clinical cases in poultry and cattle farms from Cairo Province, Egypt, outskirts belonging to first author previous research. Where those samples have been identified bio- chemically in Microbiology and Immunology Department/

National Research centre / Egypt.

2.2. Ethnobotanical

2.2.1. The shrub description and taxonomy have been done by Dr/ Nurah. Alzamel, Assistant Professor of plant ecology, Biology Department, Faculty of Science and humanities in Al Quwai’iyah, Shaqraa University, where the shrub is kept in the herbarium of the same department, Shaqraa University, with voucher number (BSQS#48) the description was compared to (Migahid, 1978, Chaudhary, 1999).

2.2.2. Traditional and therapeutic uses of shrubs were gathered from previous studies, textbooks, websites, journals, symposia, peri- odicals, and databases that manage medicinal plants used to treat human diseases in Saudi Arabia, the Arabian Peninsula, and other areas of the world. Where the accuracy of English/Arabic and Arabic/English dictionaries has been confirmed (Aati et al., 2019).

2.3. Extraction methods 2.3.1. Aqueous extraction

The aerial parts of the shrub H.salicornicum were washed three times with clean distilled water and air-dried in the shade before being ground into a powder in a blender. Cold aqueous extracts were prepared using the infusion method (Thakur et al., 2011, Nayak et al., 2011), while hot aqueous extracts were prepared using the decoction method (Thakur et al., 2011). (Shetty et al., 2008). The extracts were then stored at 4

#C until they were

required.

2.3.2. Solvent extraction

The grounded shrub H. salicornicum was extracted using (ether, ethanol and acetone) by solvent extraction method according to (Patil et al., 2009). Then the extracts were reserved at 4

#C till to

be used.

2.4. Phytochemical analysis:

2.4.1. Tannins Test:

A modified technique was employed to validate the presence of tannins in the extracts, according to (Kanife, 2012). A few drops of Ferric chloride reagent were combined with 3 ml of extract. The presence of tannins was detected by the appearance of a blue- black coloration.

2.4.2. Alkaloids Test:

To the 5 ml of extract, a few drops of Marqus reagent (made from 0.5 ml of Formaldehyde and 5 ml of concentrated H2SO4) were added. The presence of alkaloids was detected by turbidity (Ajoku et al., 2015).

2.4.3. Saponins Test:

For 5 min, vigorously shake the combination of 3 ml of H. sal-

icornicum extracts and 10 ml of distilled water in a test tube. Then

leave the test tube for 30 min to see the creation of honeycomb

foam, which indicates the presence of saponins (Cho et al., 2003,

Clarke, 1975).

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2.4.4. Flavonoids Test:

The flavonoids test was carried out following (Doss et al., 2011). A yellow tint formed when 2 ml of the extract was mixed with Alcoholic KOH (0.5 mol.), indicating the presence of flavonoids.

2.4.5. Glygosides Test:

1 ml of conc. H2SO4 was added to 0.5 g of ground H. salicornicum, which had been mixed in 2 ml glacial acetic acid with one drop of ferric chloride solution. The presence of Glycosides was indicated by a brown ring (Antia et al., 2010).

2.4.6. Anthraquinones test

A mixture of 1 g of the H. salicornicum powder and 20 ml of chloroform was heated for 5 min in a steam bath. Then the mixture while hot was filtered and let to be cool. Added equal volume of 10% ammonia solution to the filtrate. Shaking the mixture the appearance of bright pink color in the upper layer of the mixture indicated the presence of Anthraquinones (Cho et al., 2003, Clarke, 1975).

2.4.7. Sterols test

A quantity of 1 ml of H2SO4 was added to the extract. The appearance of a brownish-red ring in the contact line between the two liquids shows the presence of sterols (Bankole et al., 2016, Senhaji et al., 2005).

2.5. Antimicrobial screening

The investigation of the antimicrobial activity H. salicornicum (Ramth) aqueous and solvent extracts was done by agar well dif- fusion method against bacterial and fungal strains isolated from cattle and poultry farms (Enterococcus faecium, Shigella flexneri, Bacillus Cereus, Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, Streptococcus pyogens, Pseudomonas aerogenes, Salmonella typhimurium, and Candida albicans). Inoculated with the studied strains on petri dishes containing 20 ml of nutrient agar (for bacteria) and malt extract (for fungi) and standardized using McFarland number 0.5 to yield 1–2 X 107 cfu/ml standard inoculums. Wells of about 5 mm in diameter were drilled into the media, which were then filled with 50 l l of aqueous and solvent extracts at a concentration of 5 mg/ml. All plates were incubated for 1–3 days at 37

#C. The antibacterial efficacy was confirmed by

measuring the inhibitory zone in millimeters. Distilled water (100 l l) was employed as a negative control for antibacterial and antifungal screening, whereas the control was found to be positive for both antibacterial and antifungal screening as part of an antimicrobial sensitivity test. (NCCLS, 1993). MS Excel was used to obtain the mean and the standard deviation for each sample.

2.6. Determination of minimum inhibitory concentration (MIC) The H. salicornicum-susceptible bacterial and fungal strains were inoculated into sterile peptone water and incubated over- night at 37

#C. After that, 9 ml of sterile peptone water was added

to sterile test tubes, followed by 1 ml of the various concentrations of extracts, and finally 0.3 ml of the culture of the studied strains.

Only peptone water and the extract were used in the control. For Bacteria and Fungi, the inoculated and control tubes were incubated at 37

#C and 25#C for 24 h and 48 h, respectively, before

being checked for turbidity. The MIC was determined to be the lowest concentration that produced no turbidity (Adetitun et al., 2013).

2.7. Determination of the minimum bactericidal or fungicidal concentration (MBC/MFC)

After incubation, samples from the MIC test tubes that did not display turbidity were streaked out with sterile cotton swabs on solidified Nutrient Agar (Bacteria) and Potato Dextrose Agar (Fungi) plates and incubated at 37

#C and 25#C, respectively. After

24 and 48 h of incubation, MBC was determined as the lowest concentration of the extract that showed no growth on plates, suggest- ing bactericidal or fungicidal action (Adetitun et al., 2013).

2.8. Antimicrobial susceptibility test

The disc diffusion method was used for the analysis. Antibiotic discs were utilized (Ciprofloxacin, tetracycline, cefpodoxime, Ery- thromycin, Gentamycin, Augmentin and Nystatin). The Mueller Hinton agar plates were seeded with the tested strains before add- ing the standard antibiotic discs with sterile forceps and incubating at 37

#C and 25#C for 24 h, respectively. The antibiotics’ inhibition

zones were measured to the closest millimeters (mm) and catego- rized as susceptible (+) or resistant (

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) (Anibijuwon and Udeze, 2009).

3. Results

3.1. Ethnobotanical studies 3.1.1. The studying area

The area of the present study is Al Quwai’ valley in Al- Quway’iyah or Al Gwei’iyyah (Arabic name:

ﺔﻴﻌﻳﻮﻘﺍﻟ

) is a large Province beside Riyadh Province, Saudi Arabia. Where it is located 165 km by road southwest of Riyadh. It had a population of 126,161 people according to the 2010 census.The Province of Al- Quway’iyah is considered one of the largest provinces of the kingdom of Saudi Arabia that is located on a flat plain surrounded by mountain ranges from three sides the north and west or south, or to the west is the famous supply chain of fiery configuration, and the eastern desert is flat and wide sedimentary form, which is the desert of Hadba and the desert Jala so it represents the tip of the Arabian Shield rock. It occupies the point of convergence between the ancient pyramids and the calcareous limestone rocks, and the most famous valley in Al-Quway’iyah is Al Quwai’ valley (Wadi Al Quwai’,) which descends from the west to the east and then rush to the Hadba desert and spread through it and the name of Al-Quway’iyah derived from the name of the valley. The valley contains a lot of wild herbs and shrubs used for animal grazing, fuel and folk medicine (Fig. 1 a and b).

3.1.2. Plant description, folk uses and medicinal uses

Table 1 demonstrated that Haloxylon salicornicum (Moq) Bunge ex Boiss is a shrub of family Chenopodiaceae. Considered a promising shrub used for both man and animals in folk medicine in sev- eral countries and also used for grazing, a lot of studies confirmed its importance as antidiabetic, antioxidant, antinflam- matory, anticancer and antimicrobial for some human pathogens.

3.2. Phytochemical analysis

As shown in Table 2 and Fig. 2, all extracts contained phyto-

chemical compounds, particularly acetone and ethanol extracts,

which contained all bioactive compounds (Alkaloids, Saponins,

Tannins, Glycosides, Sterols, Anthraquinones, and Flavonoids),

whereas Glycosides, Tannins, and Flavonoids were absent in the

aqueous extract. The ether extract is devoid of flavonoids and ster-

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ols. According to the information in Table 2 and Fig. 2, the optimum solvents for extracting phytochemical compounds from the shrub H. salicornicum are acetone and ethanol.

3.3. The antimicrobial assay, MIC, MBC and

Tables 3–7 show that all types of shrub H. salicornicum extracts have significant antimicrobial activity against the examined animal pathogenic strains when compared to the standard antimicrobial. As shown in Tables 3 and 7, acetone extract has the highest significant antimicrobial activity, followed by ethanol extract, ether, and aqueous extracts. These findings, combined with those in Table and Fig. 2, revealed that acetone and ethanol are the optimum solvents for producing H. salicornicum extracts with potent antibacterial, antifungal properties.

4. Discussion

The Kingdom of Saudi Arabia is a large, waterless area with an extent of approximately 2 km that covers a substantial por- tion of the Arabian Peninsula and is characterized by diverse ecosystems and plant species (Abdel Khalik et al., 2013). Saudi Arabia’s geomorphology, like that of the Arabian Peninsula, is a primordial massif in which geologic formation developed in lockstep with the mountains. The climate in Saudi Arabia varies greatly from the coast to the interior. Along the shore, higher moisture levels are associated with milder temperatures, whereas the interior is marked by dehumidification and high temperatures (Country Profile: Saudi Arabia, 2006). Even though Saudi Arabia lacks permanent gutters or lakes, ‘‘Wadis” may be found all around the nation. In this article, the term ‘‘Wadi”

refers to a non-endless stream whose runoff is dependent on rainfall. It happens frequently, although Wadis have been underused for decades. Wadis are one of the most noticeable desert areas in arid regions, with physiographic irregularities and associated variances in plant species allocation (Kassas and Girgis, 1964). Wadi al-Quwai’a‘‘ is one of the most significant Wadis in Saudi Arabia’s Al-Quway’iyah Province’s western section. The valley is rich in wild plants and bushes

that are utilized for grazing, firewood, and traditional medicine (Fig. 1 a and b).

Saudi Arabia’s foliage is one of the most diverse biodiversi- ties in the Arabian Peninsula, with a sizable inheritable coffer of crops and medicinal plants (Rahman et al., 2004). The distribution of living forms is closely linked to geomorphology and landscape (Kassas and Girgis, 1965, Zohary, 1973, Orshan, 1986, Fakhireh et al., 2012). The structure of life forms in Wadis reflects typical desert vegetation, with therophytes and chamaephytes accounting for the bulk of species. The foliage of Wadis in general isn’t consistent; it changes from season to season, depending on the humidity level (Siddiqui and Al-Harbi, 1995). Numerous factors, such as geographical posi- tion, physiographic characteristics, and environmental effects, influence the development, maturity, renewal, and allocation of plant populations in the Wadis (Shaltout and El-Sheikh, 2003, Kürschner and Neef, 2011). Thousands of traditional plants are quietly discovered uncultivated or farmed in a variety of countries, according to ethnobotanical inspections. These plants are often ignored or neglected across the world, although they’ve been shown to have major uses on a local or worldwide level (Alatar et al., 2012). Furthermore, the majority of these plants are suitably adapted to a variety of frontier growth con- straints, such as drought or salty environments, contribute to nutrition, and play an important role in naturalistic curative therapies (Korkmaz and Ozcelik, 2013). Haloxylon salicornicum (Moq) Bunge ex Boiss is a Chenopodiaceae family shrub. The species has evolved to survive in parched locations where water is scarce and nutrients are few (Ajabnoor et al., 1984). This species is used as a meal, food aid, medicine, and in the regener- ation of waterless regions that have become degraded. As has been demonstrated in Table 1.

However, no studies have examined the antimicrobial activity

of H. salicornicum extract against microbes from animal sources,

especially because it is a grazing herb that camels and small rumi-

nants eat by instinct, and it may be used again due to Zoopharma-

cognosy, which states that animals medicate themselves by

selecting natural substances (plants, herbs, clay, and insects) to

reduce the risky effects of pathogens (Kapadia et al., 2014,

Attardo and Sartori, 2003). Especially since the aqueous extract of

Fig. 1.(a and b). This is a figure demonstrated the location of Al-Quway’iyah Province and Al Quwai’ valley in Saudi Arabia map.

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the shrub H. salicornicum is safe for medicinal purposes and may be administered without causing substantial harm (Ullah et al., 2019).

As a result, the goal of this study was to examine the ethnobotan- ical, phytochemical, and antibacterial effects of H. salicornicum against pathogens of animal origin to give this underutilized shrub more attention.

As shown in Table and Fig. 2, all extracts contained phytochemical compounds, particularly acetone and ethanol extracts, which contained all bioactive compounds (Alkaloids, Saponins, Tannins, Glycosides, Sterols, Anthraquinones, and Flavonoids), whereas Gly-

cosides, Tannins, and Flavonoids were absent in the aqueous extract. The ether extract is devoid of flavonoids and sterols.

According to the information in Table and Fig. 2, the optimum solvents for extracting phytochemical compounds from the shrub H.

salicornicum are acetone and ethanol. Much prior research has demonstrated the therapeutic value of bioactive chemicals derived from plants (Ahmad et al., 2008, Cowan, 1999, Darout et al., 2000, Eloff, 1998, Emad et al., 2009, Karaman et al., 2003, Malu et al., 2009, Mothana and Lindequist, 2005, Lin et al., 1999, Parekh and Chanda, 2008).

Table 1

This is a table demonstrate ethnobotanical ofHaloxylon salicornicum.

Ethnobotanical items References

Family name Chenopodiaceae (Shankar and Kumar, 1984, Al-Sodany et al., 2013, Shawky and

Alzamel, 2020)

Species name Haloxylon salicornicum (Shankar and Kumar, 1984, Al-Sodany et al., 2013, Shawky and

Alzamel, 2020)

Common name Ramth in Arabicﺚﻣﺭ (Al-Sodany et al., 2013, Shawky and Alzamel, 2020)

Plant life form Shrublets (Shankar and Kumar, 1984, Al-Sodany et al., 2013, Shawky and

Alzamel, 2020)

Plant specimen voucher number BSQS#48 Herbarium of Biology Department, Faculty of Science and

humanities in Al Quwai’iyah, Shaqraa University Favrouble soil for plant Salty land, mound basis, salty depressions. (Shawky and Alzamel, 2020,El-Shaer, 2010) Traditional uses for man Valuable source of food especially seeds in drought condition

in arid region

Used the plant ash for cloth washing.

Used the wood for fuel

(El-Shaer, 2010, Ali and Qaiser,2001, Al-Ani and Jawad, 1974, Singh et al., 2003).

Traditional uses for animals Used for camel and small ruminants grazing with very high concentrated food

(Shankar and Kumar, 1984, Ibnoaf, 1987, Singh et al., 2009, Ali et al., 2009, Chaudhary et al., 2003).

Folk Medicinal uses for man Used for internal ulcer healing and insect stings.

Used for dysmenorrheal by drinking as tee Used the smog for cold in Saudi Arabia.

Used for hepato-biliary treatment Used for sexual disorders

(Singh et al., 2003, Chopra et al., 1956, Anonymous,1959, Ahmad and Eram, 2011, Stewart, 1972).

Folk Medicinal uses for animals Used in some region as ethno-veterinary medicine as antiparasitic for lice, myiasis and tick treatment

Fly repellent

(Farooq et al., 2008, Raza, 2013).

Studies on plant Plant extracts have anti diabetic effect.

Antifungal effect on some human pathogenic fungi Plant derivative has lipoxygenase inhibition effect anticoagulant effect of plant extract

plant derivative has anti-tuberculosis activities plant extract has hepatoprotective effects

Free radicals isolated from plant extract have inhibition activity.

Free radicals from plant extract have antioxidants and anti- inflammatory effect

Plant extract has inhibition of uterine contraction Plant extract has antibacterial effect on some human pathogens.

Plant extract has antiparasitic effect on some animal parasites.

Increase body gain in camel as food additives.

The plant extract has anticancer effect.

The plant has antipyretic, antioxidant and anti-inflammatory effect.

(Ajabnoor et al., 1984, Shabanaet al., 1990, Ferheen et al., 2005a, Awaad et al., 2001, Ferheen et al., 2005b, Alqasoumi et al., 2012, Bibi et al., 2010, Ibrahim et al., 2011, Saleem et al., 2013, Abdallah and El-Ghazali, 2013, Saini et al., 2009, Ullah et al., 2019, Aala et al., 2021).

Table 2

This is a table demonstrate the phytochemical constituents ofH. salicornicum.

Phytochemical tests Plant extracts

H. salicornicumAqueous Extract H. salicornicumEther Extract H. salicornicumEthanolic Extract H. salicornicumAcetone Extract

Tannins Test _ + + +

Alkaloids Test + + + +

Sterols test _ _ + +

Saponins Test ± ± ± +

Flavonoids Test + _ + +

Glygosides Test _ + + +

Anthraquinones test + + + +

*(+) = presence of phytochemical compound, (_) = absence of phytochemical compound), (±) = traces.

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Additionally, Tables 3,4,5,6,7 show that all types of shrub H.

salicornicum extracts have significant antimicrobial activity against the examined animal pathogenic strains when compared

to the standard antimicrobial. As shown in Tables 3 and 7, acetone extract has the highest significant antimicrobial activity, followed by ethanol extract, ether, and aqueous extracts. These

Fig 2.This is a figure demonstrate the phytochemical profile ofH. salicornicumdifferent extracts. *0 = absence of phytochemical compound, 1 = traces of phytochemical and 2 = presence of phytochemical compound.

Table 3

This is a table demonstrate the antimicrobial activity ofHaloxylon salicornicum(Ramth)aqueous and organic extracts against some animal pathogen in (mm).

Type of microorganism Types of extract Mean ST.DEV. Control negative

Aqueous extract Organic extracts Distelled water

Hot aqueous extract Cold aqueous extract Ether Acetone Ethanol

Gram negative Ps.aerogens 0 0 0 16 24 8 11.31 0

E coli 0 0 17 36 0 10.6 15.99 0

Enterococcus 0 0 19 39 25 16.6 16.80 0

Shigella 27 0 0 18 26 14.2 13.42 0

Salmonella 0 0 16 38 23 15.4 16.14 0

Gram positive K. pneumonia 17 13 0 23 29 16.4 10.99 0

Bacillus 0 0 0 17 19 7.2 9.88 0

S.pyogens 0 0 0 19 21 8 10.97 0

S. aureus 0 0 0 18 26 8.8 12.37 0

fungi C albicans 0 0 0 23 26 9.8 13.46 0

*Mean = the average for each sample, ST.DEV. = the standerded deviation for each sample.

Table 4

This is a table demonstrate the MIC and MBC/MFC of the ether extract of theHaloxylon salicornicum.

Examined strains Concentrations of ether extract (mg/mL)

5 25 50 75 100 150 MIC MBC/MFC

E coli ++ + + _ _ _ 75 mg/ml 100 mg/ml

Salmonella ++ + + _ _ _ 75 mg/ml 100 mg/ml

Enterococcus + _ _ _ _ _ 25 mg/ml 50 mg/ml

Ps.aerogens ++ ++ + + + + _ _

Shigella + + + + + + _ _

Bacillus ++ ++ + + + + _ _

S. aureus ++ ++ + + + + _ _

S.pyogens + + + + + + _ _

K. pneumonia + + + + + + _ _

Candida albicans ++ ++ ++ + + + _ _

*(+) = turbid (microbial growth), (++) = very turbid (high microbial growth), (_) = no turbidity (no microbial grwoth).

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findings, combined with those in Table and Fig. 2, revealed that acetone and ethanol are the optimum solvents for producing H.

salicornicum extracts with potent antibacterial and antifungal properties. Much prior research (Awaad et al., 2014; Shabana et al., 1990; Ferheen et al., 2005b; Bibi et al., 2010; Abdallah and El-Ghazali, 2013; Aala et al., 2021) However, the best of our knowledge, no studies have been conducted to date that has investigated the antimicrobial effect of H. salicornicum extracts on animal pathogens, except for what has been reported for the first time in this article, so our results in addition to the previous data that have been mentioned by (Mathur et al., 2011, Saini et al., 2009) about this promising shrub that has great value as concentrates in rations of camel and small ruminants, moreover enhancing milk production, which led to the suggestion of utilizing the H. salicornicum shrub as a food supplement in poultry and cow rations.

5. Conclusion

So it could be concluded that the H. salicornicum shrub considered an important promising grazing shrub that was present naturally in arid rejoins can tolerate the salty land and inadequate water containing high value of nutrition to animals requires more attention in both veterinary and agricultural aspects and recommended to be introduced as a natural source of animal food as growth promoters and natural prophylactic antimicrobial, especially in poultry and cattle farms instead of chemical antimicrobial that were added to the animal rations in farms with no side effects or residues in animal products that also will help to decrease the bacterial resistance for human by consuming the safe animal products. Also, the results confirmed and recommended the use of acetone and ethanol as the best solvents for extraction of bioactive compounds from H. salicornicum shrub.

Table 5

This is a table demonstrate the MIC and MBC/MFC of the ethanol extract of theHaloxylon salicornicum.

Examined strains Concentrations of ethanol extract (mg/mL)

5 25 50 75 100 150 MIC MBC/MFC

E coli ++ + + + + + _ _

Salmonella ++ + _ _ _ _ 50 mg/ml 75 mg/ml

Enterococcus ++ + _ _ _ _ 50 mg/ml 75 mg/ml

Ps.aerogens ++ + _ _ _ _ 50 mg/ml 75 mg/ml

Shigella ++ + _ _ _ _ 50 mg/ml 75 mg/ml

Bacillus ++ ++ + _ _ _ 75 mg/ml 100 mg/ml

S. aureus ++ + _ _ _ _ 50 mg/ml 75 mg/ml

S.pyogens + + _ _ _ _ 50 mg/ml 75 mg/ml

K. pneumonia + _ _ _ _ _ 25 mg/ml 50 mg/ml

Candida albicans + + _ _ _ _ 50 mg/ml 75 mg/ml

Table 6

This is a table demonstrate the MIC and MBC/MFC of the acetone extract of theHaloxylon salicornicum.

Examined strains Concentrations of acetone extract (mg/mL)

5 25 50 75 100 150 MIC MBC/MFC

E coli _ _ _ _ _ _ 5 mg/ml 25 mg/ml

Salmonella _ _ _ _ _ _ 5 mg/ml 25 mg/ml

Enterococcus _ _ _ _ _ _ 5 mg/ml 25 mg/ml

Ps.aerogens ++ ++ + _ _ _ 75 mg/ml 100 mg/ml

Shigella + + + _ _ _ 75 mg/ml 100 mg/ml

Bacillus ++ ++ + _ _ _ 75 mg/ml 100 mg/ml

S. aureus ++ ++ + _ _ _ 75 mg/ml 100 mg/ml

S.pyogens + + + _ _ _ 75 mg/ml 100 mg/ml

K. pneumonia ++ + _ _ _ _ 50 mg/ml 75 mg/ml

Candida albicans ++ + _ _ _ _ 50 mg/ml 75 mg/ml

Table 7

This is a table demonstrate antimicrobial Activity of Standard Gram negative and gram positive antibiotics on the examined strains.

Control positive antimicrobial agents

Examined strains Escherichia Coli

Salmonella Typhimurium

Enterococcus Ps.

aerogens

Shigella Bacillus S.

aureus S.

pyogens K.

pneumonia

Candida albicans

Ciprofloxacin (5lg) 20 (S) 30 (S) 35 (S) 31 (S) - (R) 34 (S) 25 (S) - (R) 38 (S) Nt

Cefpodoxime (10lg) 19 (S) 16 (S) - (R) - (R) - (R) 20 (S) 27 (S) 15 (S) 22 (S) Nt

Tetracycline (30lg) 31 (S) 29 (S) 25 (S) 19 (S) - (R) 17 (S) 20 (S) - (R) 27 (S) Nt

Erythromycin (ERY) (5lg) - (R) - (R) - (R) - (R) - (R) 22 (S) 32 (S) - (R) 23 (S) Nt

Gentamycin (10lg) - (R) - (R) - (R) - (R) - (R) 25 (S) 31 (S) - (R) 29 (S) Nt

Augmentin (30lg) - (R) - (R) - (R) - (R) - (R) 14 (S) 35 19 (S) 27 (S) Nt

Nystatin (30lg) Nu Nu Nu Nu Nu Nu Nu Nu Nu 16 (S)

*(S) = Susiptable, (R) = Resistent and (Nu) = Not used.

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Declaration of Competing Interest

The authors declare that they have no known competing finan- cial interests or personal relationships that could have appeared to influence the work reported in this paper.

Acknowledgments

The authors also express their gratitude to Princess Nourah bint Abdulrahman University Researchers Supporting Project number (Grant No. PNURSP2022R103), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.

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