Supplementary file 1

(1)

Autophagy Inhibition Enhances the Mitochondrial- Mediated Apoptosis Induced by Mangrove (Avicennia

marina) Extract in Human Breast Cancer Cells

Item Type Article

Authors Esau, Luke;Sagar, Sunil;Bajic, Vladimir B.;Kaur, Mandeep Citation Autophagy Inhibition Enhances the Mitochondrial-Mediated

Apoptosis Induced by Mangrove (Avicennia marina) Extract in Human Breast Cancer Cells 2015, 5 (3):304 European Journal of Medicinal Plants

Eprint version Publisher's Version/PDF

DOI 10.9734/EJMP/2015/14181

Publisher Sciencedomain International

Journal European Journal of Medicinal Plants

Rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://

creativecommons.org/licenses/by/4.0), which permits

unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download date 2023-12-08 22:00:12

Link to Item http://hdl.handle.net/10754/550089

(2)

Esau et al.; EJMP., 2015; Supplementary material

Supplementary file 1

The sequences of the human genome based primers, probes and details of TaqMan assays used for RT-PCR

Target Sequence

p53 Forward Primer 5'-GCCCCCAGGGAGCACTA-3' p53 Reverse Primer 5'-GGGAGAGGAGCTGGTGTTG-3'

p53 Probe 5'-TTGGGCAGTGCTCGCT-3'

NF-kB Forward Primer 5'-AGCACAGATACCACCAAGACCC-3' NF-k B Reverse Primer 5'-CCAGGGAGATGCGCACTG-3'

NF-k B Probe 5'-ATCAAGATCAATGGCTACACAGGACCAGG-3' MDM2 Forward primer 5'-TTACCCAGGCTGGAGTGCAG-3'

MDM2 Reverse primer 5'-GAGAATGGTGCGAACCCG-3'

MDM2 Probe 5'-TGGCTCACTGCAAGCTCTGCCCTC-3'

BCL2 Forward primer 5’-CATGTGTGTGGAGAGCGTCAA-3’

BCL2 Reverse primer 5’-GCCGGTTCAGGTACTCAGTCA-3’

BCL2 Probe 5’-CCTGGTGGACAACATCGCCCTGT-3’

BAX Forward Primer 5'-TCCCCCCGAGAGGTCTTTT-3' BAX Reverse Primer 5'-CGGCCCCAGTTGAAGTTG-3'

BAX Probe 5'-TCAGAAAACATGTCAGCTGCCACTCGG-3'

GAPDH Forward primer 5'-AGCCTCAAGATCATCAGCAATG-3' GAPDH Reverse primer 5'-ATGGACTGTGGTCATGAGTCCTT-3'

GAPDH Probe 5'-CCAACTGCTTAGCACCCCTGGCC-3'

BAD and CASP7 Taqman Assays were procured from Applied Biosystems.

Supplementary file 2

Cell cycle distribution of MCF-7 cells treated with AM extract. MCF-7 cells were seeded in a 6-well plate at 3 x 10

⁵

cells per well and treated with 100 or 200 μg/ml AM extract for 24 h. Cells were trypsinized and stained with 10 μg/ml PI and DNA content was analyzed by

Supplementary file 1

Autophagy Inhibition Enhances the Mitochondrial- Mediated Apoptosis Induced by Mangrove (Avicennia

marina) Extract in Human Breast Cancer Cells

Item Type Article

Authors Esau, Luke;Sagar, Sunil;Bajic, Vladimir B.;Kaur, Mandeep Citation Autophagy Inhibition Enhances the Mitochondrial-Mediated

Apoptosis Induced by Mangrove (Avicennia marina) Extract in Human Breast Cancer Cells 2015, 5 (3):304 European Journal of Medicinal Plants

Eprint version Publisher's Version/PDF

DOI 10.9734/EJMP/2015/14181

Publisher Sciencedomain International

Journal European Journal of Medicinal Plants

Rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://

creativecommons.org/licenses/by/4.0), which permits

unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download date 2023-12-08 22:00:12

Link to Item http://hdl.handle.net/10754/550089

Supplementary file 1

Supplementary file 2

Cell cycle distribution of MCF-7 cells treated with AM extract. MCF-7 cells were seeded in a 6-well plate at 3 x 10

cells per well and treated with 100 or 200 μg/ml AM extract for 24 h. Cells were trypsinized and stained with 10 μg/ml PI and DNA content was analyzed by

flow cytometry. Cells treated with 200 nM Docetaxel for 24 h were used as a positive

control