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In Vitro Culture and Propagation of Punica granatum L.

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lKAU: Met.,EIIV., Arid Land Agric.

s«,

Vol. I, pp.67-75 (1410 A.H.l1990A.D.)

In Vitro Culture and Propagation of Punica granatum L.

R.S.

AL-ODIED. M.A.

SHAHEEN".

F.A.

SAAD

and A.E .

SAID

Plant Production Department, College of Agriculture, King Saud University, Riyadh, Saudi Arabia.

AIlSTIl.AtT. Shoot tip cxplarus (I em in length) of pomegranate (Punica granatumL.)proliferated and produced shoots after 8 weeks ofin vitro cul- ture.Shoot tip explants were cultured on Murashige and Skoog (MS) basal medium supplemented with a combinations ofIAA:BAat ranges of 0.0-1.3 rng/l and 0.0-3 .0mg/l respectively. Shoot multiplication occurred efficiently 4 weeks after the initial growth was recultured.BA alone (O.R mgll pro- duced the highest proliferative effect thoughBA+fAAat 3.0:0 .2mgll had a relatively high multiplication effect (3.4 shoot/explant).Theinteraction of fAA and BA showedtohave an effect on shoot length and shoot number per culture. The produced shoots were subcultured on half strength MS mediumsupplemented with 2.0mgi1 IBA and 0.25gil activated charcoal.

The percentage of motel! cultures was 100%,.

Introduction

Pomegranate (Punica granatum L.) has been cultivated for fruit production, orna- mental and medicinal purposes.

It

is propagated by vegetative parts, such as hardwood cuttings and suckers. However, few reports on in vitro shoot production of Pomegranate have been published (Jaidka and Mehra 1986, and Omura et at.

1987). Such method of propagation could reduce the time required for commercial production of large number of lines and/or stock plants from newly released cul- tivars. In vitro mass propagation has been employed for several fruit trees, including apple (Pieniazek 1968, and Werner and Boe, 1980), Peach (Hammerschlag 1979, and Skirivin and Chu 1978) and grapes (Barlass and Skene 1978). Shoot tip culture

'Currently associated with Department of Arid Land Agriculture.FaculryofMcteornlogy,Environment. and Arid Land Agriculture.King Abdulaziz University.Jcddah, Saudi Arabia.

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