Tap chi KHOA HOC DHSP TPHCM Doan Pham Nggc Ngd va Igk
PHAN TICH DA DANG DI TRUYEN CUA M O T SO GIONG DIEU (ANACARDIUM OCCIDENTALE LINN)
TRONG 6 BINH DUONG, BINH PHU'dC VA DONG NAI
BANG KI T H U A T AFLPe o A N PHAM NGOC NGA', H 6 B I C H L I E N "
TOM T A T
1 'ii'c phdn tich da dang di truyen cua cdc gidng dieu d Binh Duang. Binh Phirdc vd Dong Nai bdng kT thudt AFLP cd y nghia thiet thirc vd cdp bach cho viec bdo ve ngudn gen rd chon gidng Trong 64 td hop primer nhdn ban chon loc dd sir dung, cd 7 td hop primer cho nhieu sdn phdm khuech dai vdi mire do xudt hien da hinh rdt cao. He sd ddng dang di Iriiyan bien thien tir 0,69 den 0.92. C 'dy phd he cd rdt nhieu nhdm nhd cho thdy qudn the dieu a 3 viijig tren cd sir tap giao rdt phirc tap
Tir khda: da dang di truyen. ca} dieu. kT Ihual AFLP.
A B T R A C T
Genetic diversity analysis of some genera of cashew (Anacardium occidentale linn^
cultivated in Binh Duong, Binh Phuoc and Dong Nai provinces by using AFLP markers Genetic diversity analysis of some genera of cashew (Anacardium occidentale linn) cultivated in Binh Duong. Binh Phuoc and Dong A'a/ province by using AFLP markers has a practical and urgent meaning to pre.ierve the gene pools and select genera. Among 64 primer combinations used. 7 primer combinations give many amplification products with high polymorphism. The variation range of genetic similarity coefficients varies from 0 69 to 0.92. There are many small clusters in the genealogical tree that shows the cashew population in the above areas reveals a very complex cross-pollination existence.
Keywords: genetic diversity, cashew, AFLP markers.
1. Mo-dau chii yeu a cac tinh phia Nam Trung Bfl tir Cay diiu, Aatacardium occidentale Da Ning tra vao, Dflng Nam Bp va Tay L. thuflc lap cay hai la mim Nguyen. Theo sfl lieu thong ke kinh te - (Dicotyledoneae), lap phu Archichlamideae, xa hpi nSm 2000, dien tieh trflng dieu chu bp Sapindales, hp Xoai (Anacardiaceae), yiu tap trung 6 cac tinh miin Dflng Nam chi Anacardium, loai Occidentale. Cay Bp, trpng dp tap trung ldn nhat o ba tinh diiu dupe du nhap vap Viet Nam vao Binh Phuoc (76.437 ha), Dong Nai khoang cufli thi ki XVIII. Tuy vay, dien (36.500 ha) va Binh Duong (13.205 ha).
tich trflng diiu chi moi dupc mo rpng lit Hien nay, qua trinh phat trien cay sau nam 1983. 6 nuoc ta, diiu dupc ti-flng diiu 6 Viet Nam noi chung va Dflng Nam Bp noi rieng dang gap nhieu khfl khan va chua 6n dinh. Nguyen nhan chii yiu cua 'ThS, Trung tam Hal nhan TPHCM , . . .
"ThS, Oai hoc Binh Duong su tro ngai nay la do cac nguyen nhan
47
Tap chi KHOA HQC DHSP TPHCM Sd 36 nam 2012
nhu: san lugng va chat lugng hat dieu Ihap va khong on djnh, n5ng suat cay dieu chua cao, nhay cam vai b^nh vk con triing gay hai. MSc dii d3 co nhicu co gang nhung viec chgn giong dieu dya tren phuang phap lru\en thong de luyen chon ra nhiing d5c linh co ich nhu: kich ca hat, trpng lugng hat, chieu dai ph^l hoa... CO y nghTa rat lan nhung hi?u qua luyen chgn s5 giam Iheo lirng giai doan sinh truang phat trien ciia cay va thucmg xuyen chiu anh huang ciia moi truang.
Dimg truac tinh trang nay, viec xac dinh cac dong cha m? va dua ra cac dSc tinh CO loi vao chuong trinh chgn giong yeu cau thong tin dang tin cay han ve miic dp luang dong di truyen.
Duoi su huang dan cua TS Bui Tri, Bg mon Cong nghe Sinh hgc Thuc vat, Dai hgc Nong Lam TPHCM nhieu cong trinh nghien cim ve cay dieu da dugc thuc hien bao gom:
Do Thi Hoa, Nguyen Thi Huyen (2005) [3] [4] da khao sat linh trang canh tac cac giong dieu dang dugc trong a cac tinh trong di^u chii yeu: Binh Duang, Binh Phuoc va Dong Nai va buoc dau tim ra dugc quy trinh li trich DNA dilu nham xay dung ngan hang di truyen invitro phuc vu cho cong tac nghien cuu tinh da dang di truyin cua quan the dilu.
Pham Van Binh (2005) [2] da sir dung 4 primer de danh gia so bg muc do da dang di truyen ciia quan the dilu dang dugc trong 6 Ninh Thuan bing kt thuat RAPD. Cung voi tac gia nay da su dung 12 to hgp moi chgn Igc de danh gia su
khac bi^t di truyen ciia 4 giong dieu ban]
kT thual AFLP.
Nguyen Quynh Anh (2005) [1] d;
img dyng 2 kT thuat RAPD \a AFLI danh gii mire dp da dang di truyen cu.
cac giong dieu dang dugc trong a Ba Ri;
-Viing Tau.
Qua cac nghien ciiru dugc biel nhi da Irinh bay, nhin chung nhimg thong tin ve he gen, ve tinh da dang di truyen ca>
dieu chua dugc biet nhieu. Dac biel cac nghien curu trong nuac chi 6 miic khcri dau vai cac marker RAPD. Do do, viec su dung mgt marker hieu qua hem de tim hieu ve he gen cay dieu la mpt \ iec lam can thiet, tren co sa do ki thuat AFLP dang dugc quan tam va sir dung phuc vu cho viec bao ve nguon gen \a chon gidng.
2. Vat lieu - phirong phap 2.1. Vat lif u
Vat lieu nghien cuu gom 26 mau DNA li Inch tir la dieu thu thap tir 3 linh Binh Ducmg, Binh Phuac \a Dong Nai dugc luu trii' a - 2 0 V trong ngan hang DNA tai Trung tam Phan tich Thi nghiem Hoa sinh, Truang Dai hgc Nong Lam TPHCM.
Dua vao cac thong tin ve nguon goc va cac tinh trang ciia cac mau la dieu da dugc Ii trich DNA (bang 1), chung toi tien hanh chgn nhimg mau DNA co nguon goc tir cac giong co nhung tinh trgng dac biet. Moi tinh chgn khoang 12- 13 miu, trong do moi huyen chgn khoang 2-3 mlu CO nhimg tinh trang noi trpi.
48
r?p chi KHOA HOC BHSP TPHCM Boan Pham Nggc Ngd va Igk
Bdng 1. Nguon goc vd mot sd tinh trang ve kieu hinh ciia 26 mdu DNA nghien cim
STT
01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Ten mau DNA TBll TB28 XL 80 XL 81 TP 187 LKlOl LK 102 LK107 DQ 161 LT 158 PGS PG6 TU9 TU20 BC9 BC15 DT 1 BL16 BL23 BL28 DX3
Nguon goc TB/DN TB/DN XL/DN XL/DN TP.'DN LK/DN LK/DN LIC/DN DQ/B N LT/ON PG/BD PG/BD TU/BD TU/BD BC/BD BC/BD DT/BD BL/BP BL/BP BL/BP DX/BP
Nang sunt (Tan/ha)
2.3 2-2.5 2.2 2.5 1.5 2-2.3 2-2.3 2 5-3
2.5 1.5 1 1 0.8 1 0.8 1.1 0.8 2 1.5 3.5 2
Hinh thai la Bau ngQn la Hinh thuon Bau ngpn la BJu ngpn la Hinh thuon Bau ngon la Hinh thuon Bau ngon la Biu ngpn la BSu ngon la Hinh thuon BJu ngpn la Bau ngpn la Blu ngpn la Bau ngpn la B9u ngpn la Hinh thuon Hinh thuon Hoi Iron Hoi Iron Hinh
rinh trang ve kieu hinh Miiu sac la (la nonyia gia) Xanh nhgt/
xanh lpi Do lia/
xanh dam Xanh nhat/
xanh dam Xanh nh^l/
xanh d^m Xanh nh^il/
xanh Ipt Do tia/xanh l.?t Do tia/
xanh dam Xanh nhat/
xanh dam Do tia/
xanh dam Xanh nhat/
xanh dam Do tia/xanh dam Xanh nh^t/
xanh dam Do tia/xanh dam Do tia/xanh dam Do tia/xanh dam Xanh nhat/
xanh dam Do tia/xanh dgm Do tia/xanh dim Do tfa/xanh dam Do tia/xanh dam Do tia/xanh
Mau sac qua Do Do Vang Vang Do Do Vang Vang Do Vang Vang Vang Do Do Do Do Do Vang Co soc
Vang Do
Cac dac diem
khdc Qua chiim/
hat 16'n Qua nho/
hat lan Qua va hgt to Hat lan Qua nho/
h^t Ion Hgt r4t to Qua sang.
dep Hoa it rung Qua Ion, tron
Hat Icm, dep Vi chat Vi rat ngon Qua nhieu/
hat to Ron hat tim it sau benh Qua to gan
SQC
Qua chiim.
khong d?p Hoa na tre Hoa no loat Qua va hat to
Tap chi KHOA HOC DHSP TPHCM So 36 nam 2012
22 DX8 !>X/BP
23 PL 21 PL/BP 24 PL 33 PL/BP 25 PL 48 PL/BP 26 B D 2 3 BD/BP
thuon Hinh thuon Bau ngpn la Bau ngpn la Hinh thuon Hai tron
d^m
Xanh Co sgc nhal/\anh Igt hong Do tia/xanh
dam Xanh
nh^l/xaiih Ipt Xanh nhgt/xanh Ipt D o tia/xanh d^m
Qua va hat to Co spc it sau b?nh
Do Hoa no tre Do Hoa no logt Vang Hoa no loat
2.2, Phuong phdp
Tir nguon nguyen li?u DNA dugc li trich sEn. DNA se dugc kiem tra dp tinh sach va nong dp bang phuang phap dipn di tren gel agarose 1,4% va quang pho ke.
Nhirng mau DNA dugc chpn co ti so OD260nm/OD280nm "Sm trong khoang 1,7- 2 va nong do tren 100ng/)il.
Hoa chat diing trong phan img AFLP dugc mua ciia Applied Biosystems va 64 to hgp primer (la tat ca to hgp primer cho AFLP) dugc dat mua tai ABI.
Cdt thic DNA va chay kiem tra tren gel 1,5%: phan ung cat gom co: enzyme fcoRI; enzyme li4sel; 3X buffer T4 ligase; 300ng DNA template. U 2 gio a 37°C va lam bat hoat enzyme 6 VCC trong khoang 15 phut. Kiem tra DNA da cat bang each lay 10|il dien di tren gel agarose 1,5%.
Chuan bi hon hipp enzyme (cho 1 mau): Hon hpp enzyme can chuan bi gom co: 0,0625X buffer T4 ligase co AT;
0,0025M NaCl; 0,005mg BSA; enzyme Msel; enzyme EcoRl; T4 ligase.
Phdn wig cat va gan adaptor (cho 1 miu): IX buffer T4 ligase co ATP;
0,05M NaCl; 0,05mg BSA; lui adaptor Mse\\ \\i\ adaptor £coRI; 2^1 hon hgp enzyme (chuan bj a tren); 600ng DNA
mau. U a 2 gia 6 37"C va pha loang phan img cat gan nay bang TE IX de dugc the tich 200^11.
Nhdn ban tien chon loc: thanh phan hoa chat cho mpt phan ung nhan ban lien chgn Igc gom: 4|il san pham cua phan img cat va gan da pha loang, l^il primer nhan ban tien chgn lpc; 15^il hon hpp core mix AFLP (P/N 402005). H6n hpp phan img nay dugc khuech dai theo chucmg trinh PCR: biln tinh a 9 4 ^ trong 30 giay, h6i linh a 56"C trong 30 giay.
keo dai a 72"C trong 1 phiil, u 6 60''C trong 30 phiit va giii lanh a 4"C.
Nhdn bdn chgn loc: hon hgp phan img nhan ban chgn Igc gom: 3ul san pham nhan ban lien chpn lpc da pha loang, 5|iM primer Msel-CS'S. \\iM primer £coRI-ANN+ ch§t phat huynh quang, 15|il h6n hgp core mix AFLP (P/N 402005). H6n hgp phan img nhan ban chgn Igc dugc dem khuech dai theo chuong trinh PCR lucmg tu nhan ban tien chpn lpc. San pham nhan ban chgn lpc duoc dem dien di va thu nhan ket qua tren m^' giai trinh tu gen (sequencer ABI3100).
Xir li dii- lieu: Phan mem MSTATC dugc su dung de tuyen chpn cac to hop primer chpn loc (Primer cho kk qua khuech dai cao). Kich thuac ciia cac san
50
Tap chi KHOA HOC DHSP TPHCM Dodn Pham Ngpc Nga va tgk
pham khuech dai thu dugc sau khi dien di bang may giai trinh tu gen dugc ma hoa thanh dang nhi phan 0 va 1. Khi co san pham khuech dai thi ma hoa thanh 1 va khong CO san pham khuech dai thi ma hoa thanh 0. Bang raa hoa dupc luu duoi dang file excel va chuyen sang phan mem NTSYSpc2.1 d^xuli.
3. Ket qua va thao luan
3.1. Ket qua khdo sdt 64 to hop primer nhan ban chgn loc vd tuyen chon cdc td hop primer cho ket qud tdt nhdt
Vai 64 to hgp primer nhan ban chpn lpc tren 4 miu PG5, PG6, TU9 va TU20. Ket qua khuech dai chpn Ipc dugc trinh bay qua bang 1.
Bang 2. Ket qud khuech dai chgn loc ciia 64 to hg-p primer chgn loc trong ki thuat AFLP
Ten to liop AM-CAT/E-AAC AM-CTA/E-AGC AM-CTA/E-ACC AM-CAA/E-ACT AM-CAA/E-ACA AM-CAG/E-ACT AM-CAC/E-AOG AM-CAC/E-ACG AM-CAC/E-AAG AM-CAC/E-ACC AM-CAC/E-AGC AM-CAC/E-AAC AM-CAC/E-ACA AM-CAC/E-ACT AM-CTA/E-ACA AM-CTA/E-ACT AM-CTA/E-AAG AM-CTA/E-ACG AM-CTA/E-AGG AM-CT/VE-AOC AM-CTA/E-ACC AM-CTA/E-AAC AM-CTC/E-AGC AM-CTC/E-ACC AM-CTC/E-AAC AM-CTC/E-AGG AM-CTC/E-ACG AM-CTC/E-AAG AM-CTC/E-ACT AM-CTC/E-ACA AM-CTG/E-AGC AM-CTG/E-ACC
P r i m e r M s e l A/iel-CAT Mid-CTA Aftel-CTA .Wsel-CAA Aftel-CAA Mse\-CAG Aftel-CAC M!?I-CAC Msel-CAC Msel-CAC Msel-CAC Msel-CAC Msel-CAC Miel-CAC Mrel-CTA Msel-CTA Miel-CTA Msel-CTA Msel-CTA Msel-CTA Msel-CTA Msd-CTA Msel-CTC Msel-CTC Msel-CTC Msel-CTC Msel-CTC Msd-CTC Msel-CTC Msel-CTC Msel-CTG Msel-CTG
P r i m e r E c o R l
&0RI-AAC
&0RI-AGC
&0RI-ACC fcoRI-ACT
£coRI-ACA
&0RI-ACT
&0RI-AGG
£coRI-ACG
£coRI-AAG
£coRI-ACC
&0RI-AGC
&0RI-AAC aoRI-ACA
£coRl-ACT
£coRI-ACA
£coRI-ACT
&0RI-AAG
£coRI-ACG
£coRl-AGG
£coRI-AGC
£coRI-ACC
£coRI-AAC
&0RI-AGC
£coRl-ACC fcoRl-AAC
£coRl-AGG
&0RI-ACG
&0RI-AAG
£coRl-ACT
&0RI-ACA
£coRI-AGC
£coRI-ACC M a u iiuynh q u a n g V V V XD XD XD XLC XLC XLC V V V XD XD XD XD XLC XLC XLC V V V V V V XLC XLC XLC XD XD V V
So san pham PGS
2 0 1 14 39 15 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 31 10
PG6 0 5 1 0 0 0 3 0 0 3 1 0 0 0 0 0 0 1 1 5 0 0 1 0 2 6 0 0 0 1 29 33
khuech d a i T U 9
0 0 0 1 10 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 1 1 2 0 0 1 0 13 28
TU20 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 16 1 3 0 0 1 33 16 32
Tap chi KHOA HOC DHSP TPHCM So 36 ndm 2012
AM-CTG/E-AAC AM-CTG/E-AAG AM-CTG/E-ACO AM-CTG/E-AGC AM-CTG/E-ACA AM-CTG/E-ACT AM-CAT/E-AAG AM-CAT/E-ACG AM-CAT/E-AGG AM-CAT/E-ACA AM-CAT/E-ACr AM-CAG/E-ACG AM-CAA/E-ACG AM-CAA/E-AGC AM-CAA/E-ACC AM-CAA/E-AAC AM-CAG/E-ACA AM-ACG/E-AAG AM-ACG/E-AGG AM-ACG/E-AGC AM-ACG/E-ACC AM-ACG/E-AAC AM-CTT/E-ACT AM-CTT/E-ACA AM-CTT/E-AGC AM-CTT/E-ACC AM-CTT/E-AAC AM-CTT/E-AGG AM-CTT/E-ACG AM-CTT/E-AAG
Msel-CTG Msel-CTG ,\Ai.|-CTG AAel-CTG ,1/iel-CTG MsvUlG AAel-CAT Msel-CAT Msel-CAT Msel-CAT A/sel-CAT Msel-CAG .'Uiel-CAA A/sel-CAA Mtel-CAA Msel-CAA Msel-CAG Mscl-ACG Msel-CAG Msel-CAG Msel-CAG Msel-CAG Msel-CTT Msel-CTT Msel-CTT Msel-CTT Msel-CTT Msel-CTT Msel-CTT Msel-CTT
£eoRI-AAC
&0RI-AAO
&r)Rl-ACG
£eoRl-AGC
£c()Rl-ACA
£ef)RI-ACT
£o)Rl-AAG
£c<!RI-AC(i
&0RI-AGG
£coRI-ACA
£coRI-ACT
£coRl-ACG
£coRl-ACG
£cuRl-AGC
£coRI-ACC
£eoRl-AAC
£roRl-ACA
£coRl-AAG
£coRl-AGG
£coRI-AGC
£coRl-ACC
£coRl-AAC
£coRI-ACT
£coRI-ACA
£eoRl-AGC
£coRI-ACC
£eoRl-AAC
£coRI-AGG
£coRI-ACG
£eoRI-AAG V XLC XLC XLC XD XD XLC XLC XLC XD XD XLC XLC V V V XD XLC XLC V V V XD XD V V V XLC XLC XLC
25 3 ID 35 40 23 0 1 16 14 1 9 0 25 0 0 14 0 14 9 10 0 0 0 1 0 0 4' 0 1
34 39 37 33 1 33 2 3 0 3 7 7 0 0 0 0 12 0 5 2 8 0 1 0 1 0 1 1 0 2
23 29 26 22 12 31 0 2 1 4 10 0 3 0 0 0 31 27 37 28 23 32 0 0 2 12 0 0 0 4
30 39 27 11 39 42 0 1 0 2 11 1 1 4 0 0 25 5 10 26 10 11 1 8 0 5 0 4 0 4 Chu Ihich V: Vdng; XLC: Xanh la cdy; XD; Xanh ducmg
Chon loc bang phan mem MSTATC, chiing toi nhan t h i y chi co 7 t6 hop primer cho tren 10 san p h i m khuech dai 6 moi mau va trong s6 nay co 6 to hop primer co nguon goc tCr primer Msel-CTG va primer £coRJ-ANN. DiSu nay chung to primer tien chpn lpc MseV
Bdng 3. Danh sach 7 to hgp primer chgn loc dugc tuyen chgn C khi gan them 2 nucleotide T va G de tro thanh primer chpn Ipc thich hgp cho phan ling PCR chpn lpc tren cay diSu. Do do chiing toi chpn 7 cap primer nay cho phan tich da dang di truyen tren cac mau con lai.
Ten to hgrp Primer Msel
Primer
£coRI liuynh quang
So san pham khuech d^i PGS PG6 TU9 T l S AM-CTG/E-AGG Mse\-CJG E c o R I - A G C V
AM-CTG/E-ACC M s e l - C T G £ c o R I - A C C V 31 10
29 33
13 28
16 32
52
Tap chi KHOA HOC DHSP TPHCM Dodn Pham Nggc Nga va tgk
AM-CTG/E-AAC AM-CTG/E-ACG AM-CTG/E-AGC AM-CTG/E-ACT AM-CAG/E-ACA
Msel- CTG Msel- CTG Mse\- CTG Msel- CI G Msel- CAG
£eoRl -AAC
£coRI -ACG
£raRI -AGC
£coRI -ACT
£coRI -ACA . V XLC XLC XD XD
25 10 35 23 14
34 37 33 33 12
23 26 22 31 31
30 27 11 42 25 3.2. Phdn lich niiec do da dang di
truyen Iren cay dieu g 3 tinh Binh Duong, Binh Phudc vd Dong Nai
De danh gia tong quat muc dp da dang di truyen cua quan the dieu a ba tinh Binh Duong. Binh Phuac \a Dong Nai, chiing toi da tien hanh lap ca)' di truyen the hien quan he di truyen giua 26 mau dieu phan tich (hinh).
Hinh \e cho thay he so dong dang di truyen ciia 26 mau dieu phan tich bien thien tir 0.69 dSn 0,92. Nhu \ a\. cac mau phan tich co quan he di truyen kha gin nhau. Ca\ di truyen chia thanh hai nhom Ion (X \ a Y) co miic dp tucmg dong di truySn til 69% Atn 92%. Trong 26 miu diSu phan tich. nhom X chiem 8 mau va nhom Y chiSm 18 raSu. Nhom X chu yeu g6m cac miu thugc hai tinh Binh Phuoc va D6ng Nai. Nhom Y gSm cac miu thugc ca ba tinh.
Nhom X lai chia thanh hai nhom nho XI va XII co mire dp tuong d6ng di truySn khoang 70%. Nhom XI chi co hai mSu LT158 va TP187, co mire dp tuong ddng di truySn khoang 74%. Khi phan tich mire dp da dang di truySn giita cac miu thupc tinh Dong Nai thi hai mau LT158 va TP187 cung l?p thanh mpt nhom. KSt qua nay cho thiy hai miu LT158 va TP187 co thS co cimg t6 tien voi nhau. GiOa cac miu nhom XI co quan
he di truyen xa hem giira cac mau nhom XII. Do do, CO the phan biet hai mau nay vai cac mau con lai. Nhom XII gom 6 mau, CO mire dp tucmg dong di truyen khoang 77%. Cac mau trong nhom nay phan bo chu yeu tren hai tinh Binh Phucfc va Dong Nai. Nhu vay, doi vai nhom XII chi gom cac mau thupc tinh Binh Phuac va Dong Nai, khong thay su xuit hien cua cac mau thupc tinh Binh Duong. Co th§ trong qua trinh phat trien trong mai nong dan da lay giong chii yeu a Dong Nai cho nen cac miu thupc tinh Binh Phuac CO kieu gen kha gan vai cac mau tinh D6ng Nai va kha xa voi cac miu tinh Binh Duong.
Nhom Y chia thanh hai nhom YI va YII CO mire dp tuang dong di truyen khoang 70%. Nhom YI phan thanh rit nhieu nhom nho, co mire dp tuang dong di truySn kha cao (>70%). Co the nhom nay chi g6m mpt vai gi6ng nhtmg co su lai h6n tap giOa cac ca the dan den cac cay lai co ki§u gen gin giong nhau.
Trong nhom YI chiing toi nhan thay co su hinh thanh nhom giOa cac miu thupc tinh Binh Phudc va D6ng Nai, ciing nhu giira cac miu thugc tinh Binh Duong va D6ng Nai. Nhu vay, cac miu dieu thu thap a tinh D5ng Nai vira co kiSu gen gan voi cac miu thupc tinh Binh Phuoc, vira
Tap chi KHOA HOC DHSP TPHCM Sd 36 nam 2012
CO kieu gen gan voi cac mau tinh Binh Duang.
PL21 vii PL48 la hai mau co kieu gen kha giong nhau (chi khac bl^l vc di truyen khoang 8%), Khi phan tich muc dp da dang di truyen cua cac mau dieu
thu thap a tinh Binh Phirac, chung toi cijng nh^n lh4y hai mhx PL21 va Pl.48 c6 mi'rc dp tuang dong di truyen kha cao.
Nhu v^y hai c^ the nay co the thugc mgt nhom qu^n the vi chung co quan he di truyen kha gan nhau.
Hinh. Cdy di truyen cua 26 mdu dieu thu va Dong Nai tren 4. Ket luan
TLT cac ket qua tren, chung toi co the rut ra mot so ket luan ve moi quan he di truyen giiia 26 ca the cay dieu 6 Binh Ducmg, Binh Phirac va dong Nai dua tren 7 to hgp primer chpn lpc nhu sau:
26 mau dieu phan tich co he so dong dang di truyen bien thien tu 0,69 den 0,92. Dieu nay chung to giua cac mau CO quan he di truyen kha gan nhau.
Quan the di8u hien dugc trong 6 3 vung Binh Duong, Binh Phuac va ddng Nai CO miic dp khac nhau ve di truyen khoang 28%, cho thay nguon gen cay dieu a day rat ngheo.
Co su khac biet rat lan giua tinh da hinh ve kieu gen cua cac ca thi dilu tai 3 tinh Binh Duong, Binh Phuac va Dong
thap a 3 tinh Binh Dircmg. Binh Phirac 7 to hgrp primer
Nai va tinh trgng kieu hinh thuc te. Do do can phai nghien cOni kT m6i quan he giira kieu gen va kieu hinh de co ca so chpn giong phu hgp va hieu qua.
Quan the cay dieu lai tinh Dong Nai CO muc dp phan bo rgng nhat tren cay di truyen va c6 muc dg tucmg dong dl iruyen kha cao vai quan the dieu cua tinh Binh Phuac \a Binh Ducmg. Dieu nay cho thay quan the dieu cua tinh Dong Nai CO the CO kha nSng thich nghi cao hon voi nhiJng dieu ki|n canh tac cua cac tinh khac. Do do cong tac chgn giong nen tap trung vao tinh nay.
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Tap chi KHOA HOC OHSP TPHCM Dodn Pham Ngpc Ngd vd tgk
TAI LIEU THAM KHAO
1. Nguyen Quynh Anh (2005). Ddnh gid .u> bg mire do da clang cii truyen cua qudn the dieu (Anacardium occidentale L) hien dugc trdng tai tmh Bd Rio - Vung Tau bdng kT thuat RAPD vd .-IF/,/'. Luan van tot nghiep kT sir cong nghe sinh hpc Dai hpc Nong lam, TP H6 Chi Minh,
2. Pham Van Binh (2005). Ddnh gid s(f hd mire dg da dang di iruyen cua qudn the dieu (Anacardium occidentale L.) hien dirge trdng lai linh Ninh Thudn bdng ki thudt RAPD vd AFLP. Luan \an tot nghiep kT su cong nghe sinh hpc Dai hpc Nong lam, TP H6 Chi Minh.
3. D6 Thi Hoa (2005). Buoc ddu diSu tra hien trgng canh tdc vd xdy dung ngdn hdng di truyen invitro cdc gidng dieu a mgt so hiiy^n irong dieu chinh ciia tinh Binh Duang, Luan van tot nghiep kT su nong nghiep Truang Dai hgc Nong lam, TP Ho Chi Minh.
4. Nguyen Thj Huyin (2005). Budc ddu dieu tra bien trgng canh tdc vd xdy dimg ngdn hdng gen cdy dilm tai mgt sd huyen ciia tinh Ddng Nai. Luan van t6t nghiep kT su nong nghiep Truang Dai hpc Nong lam, TP Ho Chi Minh.
(Ngiy Toa soan nti$n diroc bdt 01-3-2012, ngdy chSp nti$n dSng 24-4-2012)
