\j NHAN GIONG CAY DANG SAM (Codonopsis javanica (Blume) Hook. f. et Thomson)
BANG KY THUAT NUOI CAY M O
Bui Van Thing', Cao Thi Viet Nga^ Vui Van Kien^, Nguyin Van Viet'' ' • Truong Dai hoc Lam nghiep
TOM TAT
Quy trinh nhan giong cay Dang sam bang ky thuat nuoi cay mo da dugc nghien cmi thanh cong. Ket qua nghien cim cho thay, sat khuan be mat mau cay bang con 70% trong 2 phiit va khii trung bang sodium hypochlorite (NaClO) 8% ffong 15 phut cho ty le mau sach nay mam 70%. Cam ling tao cum choi tren moi tnrong MS bo sung 0,5 mg/1 kinetin, 0,2 mg/1 NAA, 30 g/1 sucrose va 7 g/1 agar cho he so nhan choi 16,55 lan/chu ky nhan (3 tuan), ty le choi hihi hieu {>2,5 cm) la 91,09%. Choi ra re 100%. so re trung binh 6.17 re/cay va chieu dai re trung binh 1,2 cm khi nuoi tren moi truong MS bo sung 0,3 mg/I DBA, 20 g/1 sucrose va 7 g/1 agar. Cay con hoan chinh dugc huan luyen \'a chuyen ra trong tren gia the 100% cat vang cho ty le cay song 98,89%, chieu cao cay trung binh 11,07 cm va chieu dai re trung binh 13.51 cm, cay sinh tnicmg va phat trien tot sau 4 tuan trong. Quy trinh nhan giong nay c6 the ap dung de san xuat cay giong Dang sam chat lugng tot, dap ling nhu cau nguon cay giong Dang sam hien nay.
Tir khoa: Cay Dang sam, cum choi, nhan giong, nuoi cay mo.
L DAT VAN DE
Dang sam (Codonopsis javanica (Blume) Hook. f. et Thomson) la cay thuoc quy, c6 gia tri dugc lieu cao (Sach do Viet Nain, 2007), bo phan sii dung chinh la re cij c6 thanh phan saponin cao, nhieu loai axit amin va cac chat vi lucmg, v.v.. (Hoang Minh Chung, Pham Xuan Sinh, 2002 ). Re cii cay Dang sam c6 tac dung tot khi sir dung lam thuoc bo mau, tang hong cau, chira ca the suy nhugc, vang da, an uong kho tieu.
Hien nay, nhu cau ctia con nguoi ve nguon dugc lieu phuc vu cham soc sue khoe va chira berth ngay cang tang. Nguon dugc lieu con nguoi dang sir dung c6 the dugc tong hop bang nhieu con duong khac nhau nhu tong hop hoa hoc, tong hop tix vi sinh vat, song nguon dugc lieu tij thuc vat da dugc con nguoi sii dung tir lau va nhu cau ngay cang Ion. Tuy nhien, cac loai cay dugc lieu trong tii nhien dang hi giam ve so lugng va chat lugng bai su khai thac qua muc cgng vdi cac dieu kien ngay cang bat Igi ciia moi truong tu nhien do bien doi khi hau, dan den nhieu loai cay dugc lieu quy hiem hi tuyet chimg, mot so loai dang hi de dga tuyet chiing ngoai ty nhien, anh huong den nguon
cung cap dugc lieu ben vting cho con nguoi.
Mac du hien nay Nha nuac ta da rat quan tam den van de nay, c6 nhirng chij truong bao ve nguon dugc lieu quy. Nhimg rimg ty nhien bi tan pha nghiem trong, nguon dy tnJ trong tu nhien ngay cang can kiet. Chinh vi vay ma nguon dugc lieu tir cay Dang sam trong tu nhien ngay cang it trong khi nhu cau ngay mot tang. De c6 the cung cap nguon dugc lieu Dang sam chat lugng tot, ben vimg dap tmg nhu cau cham soc siic khoe ciia con nguoi, can phai gay trong vimg dugc lieu cay Dang sam.
Trong quy trinh trong cac loai cay thuoc noi chung va cay Dang sam noi rieng, giong tot quyet dirth den nang suat \ a dac biet la ham lugng va hoat tinh dugc lieu trong san pham sau thu hoach. Ben vay, viec ap dung cac ky thuat tien tien vao nhan giong cay Dang sain de san xuat cay giong chat lugng cao la rat can thiet.
Bai bao nay cong bo ket qua nghien ciru nhan giong thanh cong cay Dang sam bang ky thuat nuoi cay mo dat he so nhan giong cao.
n. VAT LIEU, PHirONG PHAP NGHIEN CUXJ 2.1. Vat lieu nghien ciru
- Hat Dang sam chin thu hoach dugc thu tii cay dau dong da tuycn chgn tai vuon cay thudc Tam Dao. VTnh Phuc.
TAP CHI KHOA HOC VA CONG NGHE LAM NGHIEP SO 4-2016
Cong nghe sinh hpc & Giong cay trong - Cac loai moi truong nuoi cay dugc ghi 6 bang \.
2.2. Phinmg ph4p nghien ciru
- Nuoi cay khei dong: Hat sau khi nia sach bing nuoc xa phong loang, sat khuan be m5t qua bang c6n 70% trong 2 phut, sau do khu trijng diet khuan va nam bing dung dich NaClO 8% trong thai gian 15 phiit, mau duoc trang bing nude cat v6 trung 5 Ian va tham kho bang giiy thSm v6 trimg. Cay hat len moi truong nuoi ciy khoi dong (MTKD), nuoi cSy duoi anh sang gian den neon. Sau 4 tuan nuoi, hat nay m§m thanh cay con dat chieu cao tir 5 - 8 cm dugc sii dvmg lam vat lieu cho giai doan nghien cmi nhan nhanh choi.
- Nhan nhanh chdi: Than cay Dang sam in vitro dugc cSt thanh cac doan c6 kich thuoc 1,0 1,5 cm loai bo phdn la va cSy len moi tnxcmg tao cum ch6i (Mi-Si. 9) c6 ham Itrgng chat diSu hoa sinh truong (DHST) khac nhau.
MIU dugc nuoi duoi anh sang gian den neon, sau 3 tuSn nuoi mlu tao cum choi thong ke so choi tren cum choi, choi hihi hieu (chieu cao >
2,5 cm) va tinh he s6 nhan choi.
Tao cay hoan chinh: Chgn ciim choi phat trien dong deu, dung keo cat cac choi huu hieu CO kich thudc khoang 2,5 cm va cay len moi truong kich thich choi ra re tao cay hoan chinh (M-R). 5). Cac binh ch6i dugc nuoi duoi anh sang gian den neon, sau 4 tuan nuoi choi ra re tao cay con hoan chinh, thong ke so re tren
choi va do chiSu dai re bang thuoc.
- Huan luyen va ra ngoi: Cac cay con ra re in vitro dugc dua ra nha huSn luyen cay mo trong thai gian 7 ngay dl cay thich nghi din voi diSu kien moi tnicmg tu nhien. Sau thoi gian hu§n luyen cay con in vitro cung cap (than cay khoe, c6 mau xanh tim, la to va day, b6 rl phat triln). Cay con dugc rura bg re loai bo moi tnicmg thach bang nude may (nia nhe nhang tranh lam gay re, dap than, la cay). Sau do, cay con dugc ciy tren cac cong thuc gia thi khac nhau (GT1.5), cay dugc tre anh sang chieu true xa bang ludi den (50%), ngay tudi nude phun suong 4 Ian (2 Ian vao buoi sang va 2 lin vao bu6i chiSu), moi l4n 5 phiit. Sau 4 tuan trdng, th6ng ke s6 lieu ty le cay song, chiSu cao cay, chieu dai re va danh gia chat lugng cay.
Cac thi nghiem dugc bo tri trong cac binh tru thiiy tinh (5 mau/bmh 200 ml). Moi cong thiic nhac lai 3 lin. S6 lieu dugc xu ly bSng phSn m&n Microsoft Excel va phuomg phap Duncan's test (Duncan, 1995) vdi miic sai khac CO y nghia p = 0,05.
DiSu kien phong nuoi cay: nhiet do phong nuoi 25 ± 2°C, cuong do anh sang gian den neon 3.000 Lux, thai gian chieu sang 14 h/ngay.
Cac loai moi truong nuoi cay trong nghien ciiu dua tren moi truong dinh dudng co ban MS ciia Murashige va Skoog (Murashige T.
and SkoogF. ,1962).
Bang \. Th^nh phan cac loai moi trvorng nuoi cay cay Dang sam Thanh phan m6i tnrdrng nuoi cay MS bo sung 30 g/1 sucrose , 7 g/1 agar.
MS b6 sung (0,1 - 0,75 mg/1) BAP, (0,2 -0,75 mg/1) kinetin, (0,1 - 0,3 mg/1) NAA, 30 g/1 sucrose, 7 g/1 agar (bang 2)
MS b6 sung (0,25 - 0,75 mg/1) NAA, 0,3 mg/1 IBA, 20 g/1 sucrose, 7 g/1 agar (bang 3).
GT,: 100% dat tang B; GTj: 75% &ix ting B - 25%
cat vang;: 50% dit ting B - 50% cat vang; GT4: 25%
d4t ting B - 75% cat vang; GTj: 100% cat vang.
Giai doan nuoi cky
Nuoi cay khoi dong Tao cirni choi
Kich thich choi ra re tao cay hoan chinh Ra ngoi
Ky hieu moi trirong MTKD Mi-S,_io
M-R1.5 GT,.5
TAP c m KHOA HOC VA CONG NGHE LAM NGHIEP SO 4-2016
Tat ca cac moi truong nuoi cay dugc chuan do d£n pH = 5,8; khu trimg d 121°C, ap suat
1,5 atm trong 20 phiit.
HL KET QUA NGHIEN CUU, THAO LUAN 3.1. Nuoi cay khoi dong cay Dang sam
Hat Dang sam sau khi khu trung cay tren moi truong nuoi c§y khdi dong (MTKD: MS bo sung 30 g/1 sucrose, 7 g/1 agar), nuoi cay dudi anh sang gian den neon. Sau 4 tuan nuoi, ty le hat Dang sam nay mam thanh cay con dat 70%, ty le hat bi nhilm khuAn 17,8% va ty le hat khong nay mam 12,2%. Cay con dat chieu cao tir 5 - 8 cm dugc sii dung lam vat lieu cho giai doan nghien ciiu nhan nhanh choi.
3.2. Nhan nhanh choi cay Dang sam
Thi nghiem nhan nhanh choi cay Dang sam in vitro tren 10 cong thuc thi nghiem vdi loai va ham lugng chat DHST khac nhau. Ket qua thu dugc trinh bay nhu d bang 2, cho thay rang:
tren cong thiic moi truong bo simg loai va ham lugng chat DHST khac nhau anh hudng ro ret den kha nang tai sinh choi cay Dang sam, he so nhan nhanh choi dao dong ttr 1,3 den 16,55 lan/chu ky nhan (3 tuan nuoi) va ty le choi hiru hieu (> 2,5 cm) dao dong tir 66,73 91,09%.
Trong khi do, d cong thiic doi chiing (DC) chi cho he so nhan choi rat thap (1,3 lan/chu ky nhan) va ty le chdi hihi hieu chi dat 45,38%.
Ket qua thu dugc ciing chi ra rang, nuoi cay cay Dang sam in vitro rat nhay cam vdi loai va ham lugng chat DHST. Trong cac cong thiic thi nghiem bo sung ham lugng chat DHST rit it (0,1 - 0,75 mg/1) nhtmg da tac dong ro ret den kha nang tai sinh choi. Nhan thay moi truong dinh duong MS co ban bo sung 0,5 mg/1 kinetin va 0,2 mg/1 NAA cho he so nhan choi va ty le choi hiru hieu dat cao nhat trong cac cong thiic thi nghiem (16,55 ISn/chu ky
nhan va 91,09% choi hiru hieu).
Theo thong bao ciia Nguyin Hoang Uyen Dung (2012) da nuoi cay choi cay Dang sam in vitro trong cac loai moi truong khac nhau, ham lugng sucrose, ham lugng nude dira va nong do BAP khac nhau. K6t qua cho thdy, chdi Dang sdm nuoi tren moi truong MS bo simg 4%) sucrose, 10%) (v/v) nude dua va 0,5 mg/1 BA cho kit qua nhan chdi la 3,7 chdi/cum.
Tuong tu, tac gia Doan Trong Due, Tran Van Minh (2015) thanh cong trong viec dong hoa cay Dang sam Viet Nam (Codonopsis javanica (Blume) Hook. f. et Thorns.) in vitro. Mlu nuoi cSy la dot than dugc khu triing bang HgCh 0,1% trong 5 phiit dat hieu suit vd trimg 58,3%. Moi truong nuoi cay MS cd bd sung 2 mg/1 2,4D va 0,1 mg/1 TDZ thich hgp cho sir tao mo seo tren be mat cat cila dot than sau 30 ngay nuoi cay. Md seo dugc nuoi cay tang sinh tren moi truong 1/2 MS bd sung 2 mg/1 2,4D va 0,1 mg/1 TDZ. Khdi md seo cd dudng kinh 5 mm dugc nuoi cay tren moi truong MS bd sung 2 mg/1 BA va 0,5 mg/1 NAA phat sinh cum chdi (3-5 chdi/cum) sau 60 ngay nuoi cay.
Zhang va cs (2013) thong bao nhan gidng loai sam Codonopsis pilosula, chdi nudi cay tren moi truong MS bd sung 1,0 mg/1 BAP, 0,5 mg/1 NAA, 3% sucrose, sau 9 tuan ty le chdi tai sinh tao cum chdi 75% va 15-18 chdi/cum.
Qua cac ket qua nghien ciiu ciia cac tac gia da thong bao, trong nghien ciiu nay cho thSy chdi cay Dang sam nudi cay tren moi truong MS bd sung 0,5 mg/1 kinetin va 0,2 mg/1 NAA cho he so nhan chdi va ty le chdi hihi hieu dat (16,55 l§n/chu ky nhan va 91,09% chdi hihi hieu) sau 4 tuin nudi, cao hon so vdi cdng trinh da thong bao trudc day.
TAP CHI KHOA HQC VA CONG NGHE LAM NGHlEP SO 4-2016
Cong nghi sinh hpc & Giong cay trong
Bang 2. Anh hudng
CTTN
DC Mi-Si Mi-Sj Mi-Ss Mi-S4 Mi-Ss Mi-S6 Mi-S, Mi-Sg Mi-S, Mi-S,o
cua ch4t DHST den kha nang tai sinh choi cay Dang Ham Ivgnng chat dieu hoa
sinh tnramg (mg/I) Kinetin
0,20
0,25 0,50 0,50 0,50 0,75
BAP
0,10 0,20 0,30 0,50 0,50 0,50
0,50 0,75
NAA
0,10 0,10
0,15
0,20 0,20 0,25 0,25 0,30
Hf so nhSn choi (lan/chu ky)
1,30 8,24 2,72 5,34 13,56
8,73 2,15 16,55
3,71 3,30 7,82
sam in vitro Ty \i cbbi
bihi hi^u
(%)
45,38 85,44 79,59 66,73 83,69 76,72 73,29 91,09 72,20 76,95 87,49
Ghi chu: Chdi hihi hieu la chdi cd chieu cao
> 2,5 cm, chdi tot, than va la xanh khdng bi di dang, cd kha nang cat chdi tao cay hoan chinh va nhan nhanh chu ky tiep theo.
3 J . Kich thich choi ra re tao cay hoan chinh Cac chdi hihi hieu cd chieu cao > 2,5 cm dugc cat va cay len mdi truong ra rl cd bo sung chat DHST NAA va IBA vdi cac ham lugng khac nhau. Sau 4 tuSn nudi kit qua thu dugc trinh bay d bang 3 cho thiy: Mdi truong MS khdng bd chat DHST (DC) chdi Dang sam khdng ra re sau 4 tuan nudi cay, ngugc lai tren
cac cdng thiic mdi truong MS bd sung (0,25 - 0,75 mg/1) NAA hoac 0,3 mg/1 IBA chdi Dang sam nudi cay in vitro cho ty le chdi ra re cao, dao dong tir 87,45% den 100% va chilu dai re trung binh dat 0,55 - 1,2 cm, sd re trung binh/cay dat 6,17 - 18,61 rl/cay. Ty le chdi ra re, chieu dai re va sd rl trung binh/cay dat cao nhat la d cdng thiic mdi truong M-R4 bd sung 0,3 mg/1 IBA trong cac cdng thiic thi nghiem.
Dieu nay nhan thay riing chit DHST IBA cd anh hudng tdt hon NAA din kha nang ra rl ciia chdi Dang sam nudi ciy in vitro.
Bang 3. Anh hu-frng cua chdt DHST den kha nang ra re choi Dang sam in vitro
CTTN DC M-R, M-R2 M-R3 M-R, M-R5
NAA (mg/1)
0,75 0,50 0,35
0,25
IBA (mg/1)
0,30
Ty le ch6i ra re (%)
0,00 94,22 94,08 96,53 100,00
87,45
Chieu dai re TB (cm)
0,75 0,73 0,56 1,20 0,55
Sd re trung binli/cay
7,76 8,62 17,07 18,61 6,17
TAP c m KHOA HQC VA CONG NGHE LAM NGHIEP S 6 4-2016
So sanh vdi kit qua nghien cuu nhan gidng loai sam Codonopsis pilosula cua Slupski va cs (2012) sii dung mdi truong cai tien MS bd sung 60 g/1 sucrose va 0,5mg/l IBA cho ty le chdi cam ling ra re chi dat 98%. Trong khi do, ket qua ciia nghien cuu chiing tdi, sir dung mdi truong MS bo sung ham lugng sucrose thap hon (20g/l moi truong), ham lugng chat DHST it hon (0,3 mg/1 mdi truong) nhung ty le chdi cam ling ra re dat 100%, vdi chieu dai re trung binh la 1,2 cm va so lugng re trung binh dat
18,61 re/cay.
3.4. Huan luyen va ra ngoi
Sau khi huan luyen, cay con dii tieu chuan se dugc nira sach thach va trong vao gia the tai vudn ugm. Cay Dang sam in vitro dugc trong ra vudn uom ngoai chi tieu cho ty le song cao Cling can co kha nang sinh truong tdt. 0 giai doan dau khi dua cay in vitro ra trong, thanh phan mot bau co vai tro quan trong giiip cay cd kha nang hut nude va chat dinh dudng, dong thai
khdng lam thdi re cay la rit quan trong. Trong thi nghiem nay, thanh phin rudt b4u trong cay con Dang sam nudi ciy in vitro dugc bd tri vdi 5 cdng thiic. Sau khi trong 4 min, thong ke cac chi tieu dl danh gia ty le sdng va su sinh trudng ciia cay con sau khi trong d vudn uom dugc tong hgp d bang 4.
Kit qua thu dugc cho thiy rang, gia the mot biu trong cay Dang sam nudi cay in vitro giai doan 4 tuin diu thich hgp nhat la cat vang 100% cho ty le cay sdng 98,89%, chilu cao cay trung binh 11,07 cm va chieu dai re trung binh 13,51 cm, cay cd chit lugng tdt. Sau 4 tuin cay sdng dugc chuyen ra dat trong san xuit. Theo bao cao ciia Doan Trgng Dtic va Trin Van Minh (2014), cay Dang sam nudi cay in vitro khi ra ngoi trong tren cat chi dat ty le sdng 85,07%. Kit qua nay cd the nhan dinh ring ty le cay sdng ngoai phu thuoc chinh vao thanh phin gia the rugt bao, con phu thudc vao chit lugng cay md, ky thuat cham soc, khu vyc ra cay, miia vu.
Bang 4. Anh hu-ong ciia thanh phan root hiu den kha nang song, sinh tru-ffng ciia cay Dang sam nuoi cay in vitro
CTTN Thanh phan gia the ruot bau
So lu-ffng
cay
Tyle song (%)
Chieu cao cay TB
(cm)
Chieu dai re Chat lu-ffng TB (cm) cay con GT,
GT2
GT3
GT4
GT5
100% dat tang B 75% dit ting B -
25% cat vang 50% dat ting B -
50% cat vang 25% dit ting B -
75% cat vang 100% cat vang
90 90
90
90
90 0 0
0
41,:
98,!
,32
11,07
6,45 13.51
Chu thich: +++: cay con chat lugng rat tdt (cay cung cap, than khoe, la, re phat trien rat tdt);
++: cay con chat lugng tot (cay con cung cap, than cung, la, re phat trien kha); - : cay chet.
TAP CHI KHOA HOC VA CONG NGHE LAM NGHIEP S 6 4-2016
Cong nghi sinh hpc & Giong cay trong
Hinh 1. Cay Dang sam nhan giong in vitro. a,b,c - cum choi; d,e - choi ra re tao cay hoan chinh;
f - cay trong trong gia the cat vang sau 4 tuan tuoi; g - cay trong trong dat.
r v . K E T L U A N
- Hat Dang sam chin thu hoach sau khi rira sach bang nude xa phong loang, sat khuan be mat qua bang con 70% trong 2 phiit, khu triing diet khuan va nam bang dung dich NaClO 8%
trong thdi gian 15 phiit, nudi tren mdi trudng MS bd sung 30 g/1 sucrose, 7 g/1 agar cho ty le mau sach nay mam thanh cay la 70%.
- Mdi trudng nhan nhanh choi Dang sam:
MS bo sung 0,5 mg/1 Kinetin, 0,2 mg/1 NAA, 30 g/1 sucrose va 7 g/1 agar, cho he sd nhan chdi 16,55 lan/chu ky nhan (3 man); ty le choi hihi hieu dat 91,09%.
Moi trudng nudi cay kich thich chdi Dang sam ra re: MS bd sung 0,3 mg/1 IBA, 20 g/1 sucrose va 7 g/1 agar, cho ty le choi ra r l dat 100%, sd re trung binh dat 6,17 re/cay va chilu dai re trung binh 1,2 cm sau 4 tuan nudi.
- Cay Dang sam nudi cay in vitro hoan chinh dugc huan luyen 7 ngay trong nha huin luyen cho thich nghi dan vdi dieu kien ty nhien, cay dugc trong tren gia the 100% cat vang dat ty le sdng 98,89%, chilu dai re trung binh 13,51 cm va chieu cao cay trung binh dat 11.07 cm. Sau 4 tuan. cay du tieu chuan dem trdng
san xuat.
TAI LIEU THAM KHAO
1. Doan Trong Due, Tran Van Minh (2015).
Cloning of X'iemam Dang Sam (Codonopsis Jm'anica (Blume) Hook. f. et Thoms.) in vip-o. Tap chi
Dugc lieu, tip 20, s6 1/2015.
2. Hoang Minh Chimg, Pham Xuan Sinh (2002), Budc dau tien nghien ciiu thanh phan hoa hoc cua \' thuoc Dang sam Viet Nam. Tap chi Dugc lieu, so 7 (1) 3 - 6 .
3. Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol plant, 15: 473 - 4 9 7 .
4. Nguyen Hoang Uyen Dung (2012). Vi nhan giong dang sam Codonnopsis javanica Blume. Luan van thac SI, Dai hoc Da Lat.
5. Sach do Viet Nam (2007). Phan thuc vat - Trang 152.
6. Slupski W., Ankanna S., and Bhumi G. (2011).
Microppagaiion of Codonopsis pilosula (Franch). Nannf by axillary shoot multiplication. Ada Biologica Cracoviensia Seres Bolanica, 52 (2): 87-93.
7. Zhang Y., Gao S.. Du T.. Chen H., Wang H., Zhu T., and Zhang J. (2013). Direact multiple shoot induction and plant regeneration fi-om dormant buds of Codonopsis pilosula ( Franch ). Nannf .African Jownal of Bioleclmology 10 ( 51): 10509-10515.
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M I C R O P R O P A G A T I O N
O F Codonopsis javanica (Blume) Hook. f. et Thomson
Bui Vnn Thang, Cao Thi Met Nga, \ ui \ an Kien, Nguyen Van Met SUMMARY
Procedure for micro-propagation of Codonopsa javanica (H]umc\ Hook f cl Thoinson has developed The result showed that the most suitable method for seed sterilization was soaked m elhanol 70"ij for 2 minutes and then m sodium hypochlorite (KaClO) 8"o for 7 minutes (the regeneration percentage archived 70''til. MS medium supplemented with 0.5 mgl kinetin. 0 2 mgl 1-XaphthaIencaccuc acid (N.i^.M. 30 gl sucrose, and 7 g'l agar was the most effecti\'e medium for multi-shoot regeneration (16.55 shootse.xplant). The percentage of potential shoots (which are more than 2 5 cm in length! was 91 09% 100% shoots have rooted on .MS medium contained 0.3 mgl IB,^, 20 gl sucrose, and 7 g 1 agar, with the remarkable figures being 6.17 roots'shoot and 1-2 cm root. The survival raie archived 98.X9"(i after 4 weeks of <.'.v-v»;-o acclimating and transplanting to pots of 100"() sand. The plantlels grew and de\eloped well with the a\cragc height of I 1 cm. and the length of root of 13.5 cm.
Keywords: Codonopsis javanica. micro-propagation, muUi-shoot. tissue culture.
.Nguoi phan bien N<;ay nhan bai N<;ay phan bien N<;ay quyet dinh danjj
PGS.TS. Ha Van Huan 06/5/2016
25/7/2016 30/7/2016
r.\P CHI KHOA HOC VA CONG NGHE LAM NGHIEP SO 4-2016
