KHOA HOC KY T H U A T THU Y - TAP XVIII - s 6 5 - 2011
K H A O S A T H I E U G I A K H A N G T H ^ C O A VjT CON cv SUPER M
d HAI THOI DIEM TIEM PHONG VACXIN CUM GIA CAM KHAC NHAU
LUu Hflu M3nh', Nguyfin Hifi^u Thuan^ Nguyfin Nhtft XuSn Dung^
T6M TAT
Khao sat ham Itfdng kliang thd khang virut gay bfinh cum gia cdm ci vjt con giflhg Super M, 6 hai thdi didm tifim phdng khac nhau dtfdc thtfc hifin tai Hflu Giang tir thang 1 dfi'n thang 6- 2010,
Thi nghifim ed hai nghifim thiic: NTl vjt dtfdc tifim phdng cl 1 ngay tudi va tifim lap lai liic 14 ngay tudi. NT2 tifim phdng liic 14 ngay tudi va tifim ISp lai Iiic 42 ngay tud'i. Vacxin sii dung la vacxin tai td hdp vfl hoat subtype H5, chiing Re-1 Trung Qutfc. Lilu stf dung cho vjt 1 ngay tudi la 0,3 ml, <^ vjt 14 ngay tudi tri^ Ifin la 0,5 ml. Mdi Id thi nghifim gdm 45 con, sd mdu xet nghifim la 10 mdu cl mdi tlidi difi'm la'y mdu. Thdi didm kifi'm h-a huyfi't thanh cl NTl la 33, 49, 63 va 77 ngay tudi; ci NT2 la 41, 63, 77 va 91 ngay tudi.
Hifiu gia khang thd HI dtfdc xac djnh bdng phtfdng phap lic chfi' ngtfng kfi't hdng cdu. Gia trj HI S 41og2 dtfdc xem dat miic bao hfl dfl'i vdi virut ciim gia cdm. Kfi't qua tim tha'y nhtf sau:
Vit con gidng CV Super M mflt ngay tufl'i nhan dtfdc khang thd thu ddng khang virut ciim gia cdm tir me truyin sang kha bifi'n ddng, cd hifiu gia l,81og2 ± 2,04 vdi ti lfi bao hd trong dan 23,33% va giam xufl'ng cdn 0,81og2 ± 1,3 vdi ti lfi bao hfl la 5% d ngay tudi thii 13 va khflng phat hifin dtfdc khang thd cl ngay tudi thii 21. O NTl, hifiu gia HI (Iog2) tai cac ngay tudi 35, 49,63 va 77 Idn Itfdt la: 1,0 ±0,82; 1,2 + 0,63; 1,33 ± 1,22 va 6,91og2 ± 0,82, khdng dat yfiu cdu bao hfl ddi vdi bfinh cum gia cdm la HI 5: 41og2. 6 NT2, hifiu gia HI (logj d cac thdi didm 41, 63, 77 va 91 ngay hidi Idn Itfdt la: 1,65 ± 0,86; 2,88 ± 1,67; 2,53 ± 1,34 va 2,46log2 ±1,79; ciing khdng dat yfiu cdu bao hd dd'i vdi bfinh ciim gia cdm.
Ttykhoa: Vit con super M, Ciim gia cdm, Vacxin virut H5N1, Dap ling khang thfi' The investigation of antibody leveis against avian influenza of CV super IVI
ducklings vaccinated at two different days of age Luu HCfu Manh, Nguyen Hifi'u Thuan, Nguyin Nhirt XuSn Dung
SUMMARY
The investigation of antibodies levels against avian mfluenza of CV Super M ducklings vaccinated at different days of age were carried out in Hau Ciang province. 360 ducklings were randomly allocated into 2 experiments. Each experiment consist of 45 ducklings, 4 replicates, 10 birds of each were sampled. Ducklings in experiment 1 were vaccinated at 1 day of age and revaccinated at 28 days old while ducklings of experiment 2 were vaccinated at 14 days of age and revaccinated at 42 days of age. hiactivated reassortant subtype H5 strain Re-1 vaccine against AI virus originated from China was used with the dose 0.3 ml for 1 day old duckling and 0.5 ml for 14 days or elder one. Blood samples were collected at 35, 49, 63, 77 days of age for experiment 1 and at 41, 63, 77 and 91 days of age for experiment 2. HI method was used to determine antibody of experimental birds. An HI antibody titre of > 4Iog2 is considered protective against AI virus. Results are as follows:
'Bd mfln diu y Khoa nflng nghiSp & SHUD, Dai hoc CSn Thd, -Trung tflm khuy#h n6ng Hflu Giang 'Ba mfln chan nufli Khoa nflng nghidp & SHLTD, Dai hoc Cdn Thd
KHOA HOC KY T H U A T T H O Y - TAP XVIII - s 6 5 - 2011 The maternal antibodies HI titre of 1 day old Super M ducklings was l,81og2 ± 2,04 reached to protective rate 23.33% of flocks. This value decreased to 0,81og2 ± 1,3 (5% protective rate) on the 13"' day of age and undetected at 21 days of age. Antibody titres received at 35, 49, 63 and 77 days of age from ducklings of experiment 1 were 1,0 ± 0,82; 1,2 ± 0,63; 1,33 ± 1,22 and 0,9log2 + 0,82 respectively while these values of experiment 2 at 41, 63, 77 and 91 days of age were 1,65 + 0,86; 2,88 ± 1,67; 2,53 + 1,34 and 2,461og2 ± 1,79. Duckings of both experiments have not reached protective titre against AI virus.
Key words. CV super M ducklmgs , Avian influenza. Virus HSNl vaccine, Antibody response
I. DAT VAN DE
Bfinh ciim gia cdm xay ra ci ntfdc ta vao cudi nam 2003, dfin nay bfinh vdn edn xay ra Ie te (I nhilu dia phtfdng trfin toan qufl'c va ed tinh lap lai hang nam, do do bfinh ciim gia cdm vdn cdn la vdn nan cho ngtfdi chan nufli cung nhtf cd quan quan ly. Cac cd quan chiic nang da trifin khai nhilu bien phap phdng chflng tdng hdp nham khdng chfi' dich bfinh, trong dd bifin phap tifim phdng vacxin d\S^c xem la cdng eu huTa hifiu trong vific khfl'ng che' va t i ^ tdi thanh toan dich bfinh (Td Long Thanh va cs, 2007). Trong cdng tac tifim phdng hang nam, vit dan chan nudi theo phtfdng thiic chay ddng , ngtfdi nudi phai dang ky vdi c0 quan thii y, dtfdc quan ly chat che va dtfdc tifim phdng rat nghifim ngat. Vit chay ddng la nhdm san xua't triing thtfdng pha'm, hoSc cung ca'p tning cho cac cd sd Sp
• nd di san xua't vit con.
Quy trinh tifim phdng vacxin hifin tai ap dung cho vit de la tifim mui 1 vao ngay tufli 14 va sau dd 28 ngay tifim tifi'p miii Ihii 2 (ngay tudi 42). Sau khi Hfim mui 2 la kfi't thiic, sau do 4 thang se tifim lap lai ddt 2.
Bfin canh nufli vit dan chay ddng thi mflt bd phan kha Idn nflng dan nudi mdt sd' loai gia cdm, thiiy cdm khac ban thit nhtf ngan, vjt CV Super M, ngflng, ga dia phtfdng nhd le. Do dac tinh la qui mfl nudi khflng Idn nhtf vjt dan, vific tifim phdng tifi'n hanh ciing khd khSn nfin cac ddi ttfdng nay khflng dtfdc chu y tifim phdng. Mdt sd y kifin lo ngai eiia cac nha quan ly, cac nha chSn nudi va ca cac nha khoa hoc cho rdng bfinh ciim gia cdm cd thfij xay vao thdi die'm vit eon hi 1 dfi'n 13 ngay tufl'i, khi do vit con da xua't cho ngtfdi chan nudi nhtfng chtfa dtfdc tifim phdng vacxin bao hfl.
Trong bai bao nay, chiing tdi trinh bay eac kfi't qua vl hifiu qua dap ling miln dich ctia vit CV Super M dd'i vdi vacxin ciim gia cdm d 2 thdi difi'm tifim phdng khac nhau.
n . VAT LIEU VA PHUCfNG PHAP NGHIEN CLTU
2.1 vat lifiu
- Vit thi nghiam. Vit con CV Super M mdi a'p nb. 1 ngay tufl'i tai cac cd sd a'p vit d Tiln Giang, day la nhiing vit con tti tning ciia nhiing dan vit bd' me da tifim phdng vacxin ciim gia cdm H5N1 va da xac dinh ti Ie bao hd.
Vit dtfdc chon ngdu nhien tCr 8 me a'p khac nhau. Mdi me chon 45 vit, tdng cdng 360 vit.
- Vacxin sii dung la vacxin ciim gia cdm HSNl Trung QM6C chiing Re-1, vfl boat dang nhii ddu.
Khang th^ chudn khang virut ciim subtype H5 ciia phdng thi nghifim CDC, USA.
- Khang nguydn chudn H5 cua Phdng thi nghifim CCC, Weybridge, Anh qufl'c.
2.2 Phtfdng phap - Bd'tri diinghiSm:
Vjt dtfdc bfl' b-i 2 nghiem thiic (NT) la thdi difi'm tifim phdng khac nhau:
NTl: Vjt con dtfdc tiem miii 1 Iiic 1 ngay tufl'i va tifim lap lai miii 2 liic 14 ngay tufli.
NT2: Vjt con tifim mui 1 Iiic 14 ngay tufl'i va tifim lap lai mui 2 liic 42 ngay tudi.
Ddn vj thi nghifim la dan vit 45 con. Sd ca thd xet nghifim mdu la 10 ca thd / dan. Lap lai 4 Idn.
Thdi difim la'y mau huyfi't thcinh xet nghifim: NTl: huyfi't thanh dtfdc ISy vao cac ngay tudi: 35, 49, 63 va 77; NT2: vao cac ngay 41, 63,77 va 91.
Tdng sd' mdu xet nghifim la 320 mdu.
KHOA HOC KY T H U A T THU Y - TAP XVIII - s 6 5 - 2011 Phan ling ngimg kSi Jidng cd'u (HA-
Haemagglutmation) dtf^c ditfc hifin theo quy trinh cua Cue thii y ban hanh. Quy trinh tdm tdt nlitf sau: (i) Chon 3 hang 12 gifi'ng h-ong dia microplate cd 96 gifi'ng, day hinh chtf U hoSc chir V, cd ky hifiu A, B, C; (ii) Nhd 50 ^il PBS vao gifi'ng 1 dfi'n gifi'ng 12 (hang A, B, C).
(iii) Cho 50 ^il khang nguyfin chudn vao gifing Al va Bl. (iv) Dung mieropipette trdn diu d gifi'ng Al va Bl, la'y 50 \il tir gifi'ng Al va Bl chuyfi'n sang gifing A2 va B2, trfln diu. (v) Lap lai btfdc (iv) cho dfi'n gifi'ng A12 va B12, tir A12 va B12 bo di 50 |J1. (vi) Thfim 50 [\l hdng cdu 0,5% vao ta't ca cae gifi'ng {A1-A12, B1-B12, Cl- C12), ldc nh^ bang tay. (vii) U cl 4 ^ /30-45 phiit. (viii) Doc kfi't qua khi tha'y dfl'i chiing hdng cdu Idng. Hifiu gia ngtfng kfi't la d$ pha loang khang nguyfin cao nhat cdn gay ngtfng kfi't hdng cdu.
- Plia'n dng ngdn tra nginig kit hdng cSu (HI- Haemagglutination Inhibition) dtfdc thtfc hifin theo quy trinh Cue thii y ban hanh. Quy trinh tdm tdt nhtf sau: (i) Cho 25 ^I PBS vao ta't ca cac gifing (tir A1-A12). (ii) Cho 25 ^l huyfi't thanh (mdu da xii ly) vao cac gifi'ng (Al-
A12) diing lam dd'i chiing huyfi't thanh va Bl- B12. (iii) TCr gifi'ng B1-B12, diing mieropipette trdn diu rdi la'y 25 pi chuyfin sang C1-C12, ttfdng ttf hi C1-C12 chuyfi'n sang D1-D12, lap lai dfi'n' gifi'ng H1-H12. Tir gifi'ng H1-H12 chuydn bo di 25 ^1. (iv) Cho 25 ^il khang nguyfin da pha loang vao cac gifi'ng B1-H12;
13c diu. (v) Dd yfin ci nhifit dfl phdng 60 phiit.
(vi) Cho 50 |.il hdng cdu vao ta't ca cac gifing tit A1-H12. (vii) U cl 4''C 45 phiit. (viii) Dpc kfi't qua khi tha'y dd'i chtfng hdng cdu Idng. Phan ling dtfdng tinh khi hdng cdu tu thanh cha'tn do rS day gifi'ng; hifiu gia khang thd la dd pha loang cao nha't ciia mlu huyet thanh cdn kha nSng lie chfi' ngtfng kfit h6ng cdu. Phan ling am tinh khi hdng cdu ngtfng kfi't ci day gifing.
Mdu cd hifiu gia HI 5 1/16 (4log2) la mlu dat miic bao hd vdi virut ciim gia cdm.
Sd' lifiu dtfdc xii ly gia trj trung binh X ± SD III. KET QUA VA THAO LUAN 3.1. Kfi't qua khang thfi' thu ddng d vit con theo ngay tudi
Ham Itfdng khang thd tfiu dflng ciia vit con h cac ngay tudi khac nhau trinh bay trong bang 1, 2 va 3.
Bang 1. Khang thi this ddng cua vit con d cac thdi diim 1, 13 va 21 ngay tuii
Ngay tudi 1 13 21
Sdmiu xdt nghidm (n)
40 40 40
Hidu gii tmng binh (Hi titro) 'x'(log2) ± SD
1,8 ±2.04 0,8 ± 1,30 Khdng phdt hi$n
Khodng bidn ddng 0 - 6 "
0 - 4 °
' sd'miu coflltit 4Iog2 - 6Iog2 lA 13; sd'miu khdng phat hifn khang thd la 17,
"sd'mSu CO Hl4!og21a 2;sd'miu khdng phat biSn khang thila 2S.
Bang 2. Khang thi thu ddng 6 vft 1 ngay tuii tilt cac me ip khac nha u
Mddp
1 2 3 4 T6ng so
Sd vit con 45 45 45 45 180
Sdm§u tiidm tra 10 10 10 10 40
Hidu gii tmng binh (Hi) X ilog2) t SD
0,9 ±1.45 1.8 ±2,30 2.2 ±1,75 2,3 ± 2,50 1,8 ±2.04
Khoing bidn ddng 0 - 4 "
0 - 6 "
0 - 4 ° 0 - 6 ° 0 - 6
" sd'miu CO H!tLf4hg2Ia 1. ''sd'miu co H!41og2 - 6log2la 3.
'sd'miu cd HI4log2la 4. •'sd'miu ca HI4Iog2Ii 5.
K H O A H O C K Y T H U A T T H U Y - T A P X V I I I - S O 5 - 2 0 1 1
Bang 3. Khang the"thu ddng vit con 13 ngay tud'i tii cac me Sp iihac nhau Mdap
1 2 3 4 Tong so
So vit con 45 45 45 45 180
Sd mdu xdt nghidm
10 10 10 10 40
Hidu gii tmng binh (HI) T ( t o s 2 ; ± SD
0,3 ± C.95 0,9 ±1,52 0,7 ±1,16 1,3 ± 1,49 0.8 ± 1,3
Khodng bidn ddng 0 - 3 0 - 4 ' 0 - 3 0 - 4 "
0 - 4 - sd'miu CO H!41og21a 1.
K h a n g thfi thti dflng d vjt con 1 n g a y tufli, gidng CV S u p e r M d n g u d n n a y cd miic bifi'n d d n g ra't Idn, gia tri cao nha't la 2,3. Sd' m d u k h d n g p h a t hifin k h a n g thd la 1 7 / 4 0 (42,5%);
s d ' m d u cd miic hifiu gia t h d p (<4Iog2) la 1 0 / 4 0 (25%); nhtf v a y vit c o n ed miie k h a n g thfi t h u d d n g dii bao hfl dfl'i v d i v i r u t HgN, la 32,5% (ed H I > 41og2), hifin difin hck^ d a n thi nghifim v d i ti lfi Idn Itfdt la 10%, 30%, 40% v a 50%.
6 n g a y tud'i thii 13, chi cd 2 / 4 0 m l u (5%) ciia h a i d a n cd gia tri H I = 4Iog2 , 1 0 / 4 0 m d u (25%) cd gia tri < 4Iog2 va 70% s d m l u khflng p h a t hifin k h a n g thd. Dfi'n n g a y tudi 21 thi khflng cdn p h a t hien k h a n g thfi.
Miic d d k h a n g the t h u d d n g d vit con 1 n g a y t u d i d u d e b a o h o chd'ng lai v i r u t ciim gia cdm chi chifi'm t r u n g b i n h 32,5%, cdn lai ti lfi 6 7 ^ % (tir 50% dfin 90%) ctia q u d n thd k h d n g dii k h a n g thd bao hfl, d o d o b a n klioan ciia ngtfdi c h a n n u d i c u n g nhtf n h a q u a n ly la tifim p h d n g s d m v a c x i n ciim gia cdm, n g a y
khi xua't vjt eon cho ngtfdi chan nufli, dfi giam bdt riii ro la k h a h d p ly. Dfi'n n g a y tudi thii 13 till k h a n g thd thu dflng dii bao hfl chi cdn 5%, nhtf v a y cd the xem nhtf vao thdi difim nay, vit con khflng cdn k h a n g the dfi bao hd ntfa.
Q u i trinh tifim p h d n g bdt d d u vao n g a y tufli 14 cd thd xcui la k i p tlidi giiip vit tao k h a n g thfi mdi. T u y nliifin c u n g co thfi la m u d n chang, bdi le trtfdc 13 n g a y tudi ciing cd tlid c6 mflt b' le Idn vit khflng con dii k h a n g thfi bao hfl. Do d d tim t h d i difi'm thich h d p tiem p h d n g idn ddu cho vit la cdn thifit.
3.2. Kfi't q u a d a p ling m i e n d i c h O v i t con tifim p h d n g d 1 n g a y t u d i va l a p lai d 14 n g a y tud'i
4 d a n vit, mdi d a n cd 45 con dtfdc thtf n g h i e m tiem p h d n g vacxin ciim gia cdm v a o 1 n g a y tudi va tifim l a p lai liic 14 n g a y tudi.
Hieu gia k h a n g the dtfdc k h a o sat vao cac tlidi didm 35, 49, 63 va 77 n g a y tufl'i. Ke't q u a brinh bay trong b a n g 4.
Bang 4. Hiiu gia idiang thi a vit tiSm phong vacxin cum gia cam dl ngay tudi va 14 ngay tudi
Ban vit/
so vit 1/45 2/46 3/45 4/45 Tfing so
Sdmau xet nghi0m
10 10 10 10 40
Hieu aii tmna binh IHI titre) X lloa2i ± SD Ngdy tuoi
35 1,4'±1,35
1.1±0,88 0,9 ± 0,88 1.0 ±0,82 1,0 ± 0,82
49 0,8 ± 0,92 0,9 ± 0,74 1,3 ±0,82 1,2 ±0,63 1,2 ±0,63
63 2'±1,76 0.87 ± 0,87
1,2 ±1,03 1,1 ±0.88 1,33 ±1,22
77 1,18 ±0,87
0.7 ± 0,82 1 ±0,82 0.7 ± 077 0,9 ± 0,82 '.• 1 miu coHI = 41og2 '': 1 miu cdHI=6!og2
KHOA HOC KY THUAT THU Y - TAP XVIII - stf 5 - 2011
CN
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= 3 I
2 1
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• X
• — ^ - -
- • - K h i n g Ihjihv -•— Khing Oil t u Iiimph6iui - * - KhinilhCMa
IS 30 45 60 75 9 0 Ngdy lu<fi
hu dpng vd hiiu gid tiem phdng dl vd 14 ngdy tuoi
Kfi't qua bang 1 cho tha'y d thdi difi'm 35 lap lai cl 14 ngay tudi a Long An va Ddng ngay tufli (28 ngay sau khi tifim phdng iiiiji 2)
miic hifiu gia trung binh d 4 dan la 1 log2 (tir 0,9 dfi'n 1,1 log2), chl 1 mdu dat 4log2, ti lfi bao hd 2,5% . O thdi didm 49 ngay tudi hifiu gia la 1,2 Iog2 , khdng cd mlu dat miic hifiu gia bao hd. O 63 ngay tudi hifiu gia la 1,33 log2, cd mflt mdu dat 6 log2, ti lfi bao hd 2,5%. 6 77 ngay tudi miic hifiu gia giam ehi cdn 0,9 log2.
Kfi't qua cho tha'y miic hifiu gia tang ddn tir thdi difim 35 ngay tudi dfi'n 49 va 63 ngay tudi, sau dd giam d ngay tudi thii 77. Liic nay ciing la thdi difi'm ban thit. Nhin chung d ta't ca 4 thdi didm khao sat, miic hifiu gia diu ra't tha'p so vdi miic bao hd, hay ndi khac di qui trinh tifim phdng vacxin ciim gia cdm cho vjt cl 1 ngay tudi va tifim lap lai d 14 ngay tud'i khdng tao dii khang thd bao hd.
Trong mdt nghifin cihi ciia dtf an VAHIP, tifim phdng sdm cho vit d 1 ngay tudi va tifim
Thap cho kfi't qua ti lfi bao hfl d 42 va 56 ngay tudi la 0,5% va 2% d Long An, trong khi 5 Ddng Thap la 4,4% va 2,2%. Kfi't qua tong dii nghifim ciia chiing tdi va thi nghifim nay cho tha'y tifim phdng sdm cho vjt 6 1 ngay tudi da khdng mang lai hifiu qua miln djch td't, cdn cdn nhilu nghifin ctfu tifi'p vl thdi didm tifim phdng.
3.3. Kfi't qua dap ting mifin djch d vjt con tiem phong d 14 ngay tu^i va lap lai a 42 ngay tud'i
Song song vdi thii nghifim quy trinh tifim phdng d 1 ngay tudi va 14 ngay tud'i; bdn dan vjt, mdi dan cd 45 con dtfdc thtf nghifim tifim phdng vacxut ciim gia cdm vao 14 ngay tudi va tifim lap lai liic 42 ngay tudi. Hifiu gia khang thd dtf^c khao sat vao cac thdi didm 41, 63, 77 va 91 ngay tudi. Kfi't qua trirUi bay trong bang 5 va 6.
Bang 5. Hifu gia khang thi6 v/t tiSm phdng vacxin cum gia dm a 14 va 42 ngiy tuii Bin vit/
sdvit 1/45 2/45 3/45 4/45 Tong s6
SdmSu xdt nghidm
10 10 10 10 40
Hidu aid tmna binh HI X tlaa2l ± SD Ngdy tudi 41
2,20 ± 0.92 1,33 ± 0.87 1,56 ± 0,73 1,44 ±0,73 1.65 ±0,86
63 2,90 ±1.52 3,20 ±1.48 2.90 ±1,79 2.50 ±2,01 2,88 ± 1,67
77 2,25 ±1.16 2.20 ±1.69 2.60 ±1,07 3.13 ±1,36 2.53 ±1.34
91 2.53 ±1,60 2.53 ± 1.73 2.07 ± 2,09 2,67 ±1.88 2,46 ±1,79
KHOA HOC KY THUAT THl) Y - TAP XVIII - s 6 5 - 2011 Bing 6. Phan bihiiu gia khang thi cua vit tiim phong vacxin cum gia cam
a 14 vi 42 ngay tuii Ban vit/
sdvit 1/45 2/45 3/45 4/45 Tong so
Sdmiu xdt nghidm
10 10 10 10 40
Sd mSu cd hidu aid HI s 4/002 /TV Id bio hd %l Ngdy tudi
41 1 (10%)
0 0 0 1 (2,63)
63 2(20) 3(30) 3(30) 2(20) 10(25)
77 1(10) 2(20) 2(20) 2(20) 7 (17,5)
S1 3 (33,33)
4(25) 2(20) 3 (33.33)
12 (30)
— Khdng Ih^ thu dAng
—Hifiu gii tifim phdng b 14v^42nghylu6i
— Hifiu g i i bao ho
Hieu gid tiem phdng d 14 vd 42 ngdy tuoi
Kfi't qua bang 5 va 6 cho tha'y d Ihdi diem 41 ngay tudi (27 ngay sau khi tifim phdng mui 1, trtfdc khi tifim phdng mui 2 mflt ngay), miic hifiu gia trung binh ci 4 dan la 1,65 log2 (tit 1,33 drai 2,2 Iog2), chi 1 mdu dat 4 log2, ty lfi bao hfl 2,63% . Nhtf vay nfi'u chi tifim mdt miii, va xet nghifim ci thdi didm 27 ngay sau do, thi hifiu gia khang thd khdng du bao hfl cho dan vit.
Tifim phdng lap lai ngay 42 va khao sat huyfi't thanh ci 63 ngay hifl'i, hifiu gia la 2,88 log2,.
cd 10 mlu dat hifiu gia > 4 log2, ty lfi bao hfl 25%. 677ngayhidi miic hifiu gia dat 2,53 log2.
cd 7 mdu dat hifiu gia S 4 log2, li lfi bao hfl 17,5%. 6 91 ngay hid'i miic hifiu gia dat 2,46 log2, cd 12 mdu dat hifiu gia > 4 log2, ty lfi bao hfl 30%. Kfi't qua cho tha'y hifiu gia tang sau thdi difi'm tifim KIUI 2 d 42 ngay tudi. Tir 63 ngay tudi dfi'n 77 va 91 ngay tudi, hifiu gia trung binh hdu nhtf khdng thay dd'i, nhtfng sd mdu dat hieu gia bao hd gia tang nfin ty lfi bao hd b:ong dan gia tang tit 25% Ifin 30%. Tuy nhifin d ca 4 thdi difi'm khao sat, miic hifiu gia diu rat tha'p so vdi yfiu cdu ciia miic bao hd, hay ndi khac di, dfl'i vdi vjt CV super M, tifim phdng vacxin ciim gia cdm vit con d 14 ngay
KHOA HOC KY THUAT THU Y - TAP XVIII - s 6 5 - 2011 tudi v a tifim lap lai d 42 n g a y tufl'i khflng tao
dii k h a n g thd bao hfl.
So s a n h hifiu q u a d a p ling m i l n djch dfl'i vdi q u y h:inh tifim p h d n g s d m cho vjt cl 1 n g a y hidi va Idp lai 6 14 n g a y tudi, thi q u y ti'inh tifim p h d n g ci 14 n g a y tudi va l a p lai 6 42 n g a y tudi CO cai thifin v l hifiu gia c u n g nhtf ty lfi bao hd, tuy nliifin vfln k h d n g d a p ling yfiu cdu bao h d c h o d a n v i l . Kfi't q u a n g h i f i n ciiu ciia N . K . C . T h a ' m (2008) tifim p h d n g trfin v i t S u p e r M ct 19-21 n g a y t u d i v a kifim tra huyfi't t h a n h 21 n g a y s a u d d c h o tha'y m i i c dfl b a o hfl d a t 4 5 % . S a u d d vjt dtfdc tifim l a p lai v a o 58-61 n g a y t u d i thi ty lfi b a o h d d a t dfi'n 9 0 % . C a c nghifin c t i u v l tifim p h d n g s d m c h o vjt c h o tha'y hifiu q u a d a p l i n g m i l n d j c h r d t k h a c n h a u ; n h t f n g yfi'u tfl' nhtf gifl'ng v i t , v a c x i n , t h d i difim tifim p h d n g v d n la n h i i n g yfi'u t d a n h h t f d n g dfi'n hifiu q u a m i l n d j c h v a c d n tifi'p t u c n g h i e n Cliu.
IV. K E T L U A N
Tifim p h d n g v a c x i n c i i m g i a c d m H j N , T r u n g Qud'c c h i i n g R e - 1 , v d b o a t d a n g n h i i d d u c h o vjt C V S u p e r M v a o 1 n g a y tufli v a tifim l a p lai v a o 28 n g a y t u d i c h o d a p l i n g m i l n d i c h ra't tha'p, khflng d u m i i e b a o hfl d d i v d i v i r u t c i i m gia c d m . Tifim p h d n g v a o n g a y t u d i t h i i 14 v a l a p l a i v a o n g a y tufl'i 42, hifiu gia k h a n g t h d cai thifin h d n , n h t f n g c i i n g k h d n g dii m i i c b a o h d ,
C d n nghifin ciiu t h d i d i d m tifim p h d n g thich h d p c h o g i d n g v i t C V S u p e r M d a t hifiu gia b a o h d , g d p p h d n g i a m thifi'u n g u y cd x a y r a bfinh c i i m gia c d m n g u y hifi'm.
T A I L I E U T H A M K H A O 1. Du an VAHIP.2009, H6i thao danh gia
kfi't qua tifim p h d n g thii nghiam vacxin cum gia cdm HSNl cho vjt. Tai li6u hfli thiio (khAiig xua't ban).
2. Nguyen Thi Tliu Hong, Dang Hung, Nguyen Ticn T. unj;. Do van Dung, Peter Spadbrow, Leigh Nind. Serological and immunological responses of ducklings vaccinated at 1 and 21 days of ago with lyophiliscd live duck plague vaccine. ACIAR proceedings Noll 7.
3. Dao Yfi'n Knanh, Tfl Long Thanh, Hoang Dan Pha'n, Trdn Thj H o a n , Vu Thi MJ H a n h . 2008. Kit'm nghifim vacxin ciim ga H5N2 n h a p tir Ha Lan va Trung q u d c . Tap chi KHKT thu y, l$p XV, sd'5. ,
.. Dao Yen Khanh, T6 Long Thanh. 2009.
Khao nghiCm thtfc dia vacxin cum gia d m H5N2 tir Ha Lan va Trung qutfc.
Phan 1: Dd an toan cii.i vacxin va dap utig mi^n ilich cua ga sau khi tiOm phdng. Tap chi KHKT didy t.1p XV!, sdi 5. To Long Thanh, Dao Yfi'n Khanh, 2009. Khao
nghifim ihuc dia vacxin cum gia cdm H5N2 tir Ha Lan vn Trung qutfc. Phdn 2: PhSn btf hifiu gia khang thd tr^n ga tai cac thdi di^m sau tiOm phong. Tap chi KHKT thu y, tip XVI. w 3
H. Nguyin Khdc Chung Tha'm, 2008. Khao sat hifiu gia khang thd sau tifim phdng vacxin ciim gi.i cdm va stf Itfu hanh virut cum A, H5 trfin dan gia cdm tai tinh An giang.
LuSn an Thac s i DH Cdn Thd.
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