Nghiin cdu YDHCT Viet Nam So 50-2016
N G H I E N C U t J K H A N A N G G A Y K I C H t T N G N I E M M ^ C H A U M 6 N T R U ' C T R A N G C U A T H U O C N G A M T R l A T T T R E N T H O T h e p o s s i b i l i t y o f c a u s i n g a n o r e c t u m i r r i t a b i l i t y i n r a b b i t s o f A T T e x t r a c t
" N g u y e n H o n g S o n ', ** B a u X u a n C a n h ' , ** P h ^ m T h j VSn Anh ^ Hoc Vien YDuac hoc c6 nnryin Vict Nam. ^ Tnedng Dgl hoc T Ha Nql
Mgc tieu: ddnh gia kha ndng kich trng niem mgc hgu mon true 0-ang cita ATT. Phinmg phip;
Xac dlnh Ilnh kich ting cdp dnh nlim mac hgu mon Irtfc 0-ang Irin Ihd. cac phan ting nghiim Irgtig cita niem mgc hgu mon khi liip xtk vcti ATT. Danh cac ldn Ihuong vi, cac phan ting cm niem muc hgu mon mrc trang bdng guan sat dgi thi vit vi Ihi (kinh hiin vl) thong qua cac chi si nhi, sung huyil phiL Diim Irung bmh vi xung huyit va phi, ni dirge ghi lgi. Kit qua: ATT gay kich ling nhe nlim mac hgu mon Uvc Irang. Kit lufn: ATTco thi iugc sir dung Iren Uim sdng
Tir khoa: ATT, kfch tag bau mdn t r g t t r i n g , thd.
Summaiy
T H E P O S S I B I L I T Y O F C A U S I N G A N O R E C T U M m R I T A B I L I T Y I N R A B B I T S O F A T T E X T R A C T
Objectives: This study was aimed al evaluating anorectum Irrilation in rabbits of ATT extract Method: to determine the acule anorectum irrilation and severe anorectum reactions in rabbits wm treated with Ihe ATT extract Anorectal Injuries and reactions were examined by microscopic morphology uslnganoreclal congestion and edema criteria Sesulls and Conclusion: Ihe ATT extract caused anorectum Irritation with mild degree. This remedy may has clinical applications.
Keywords: ATT, anorectum irritation, rabbits.
Dja chi lien hf; Ph9m Thj Van Anh - Trutme Dai hnr V Hi MA: . . . Oi. chi .mail; phamthiv,„ari,.hn,u@eSLm ' ^^' ° j ! ° "J^ ™ ' Z S l t Phin bijn khoa h,c: GS.TS. Nguyfi wTuoc i K > ' S""" " ' ^ " ^ ' " ' ™ "
• BS; •• TS.BSCtai " s i y Hing hSo; 27/9/2016
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Journal af research In vielnam traditianal medicme and pharmacy No 50-2016 I. D4T VAN DE
Btah Tri la mgt ttong nhimg benh thudng gap d vtog hau mdn ttirc tttag.
Theo Nguyen Manh Nhtoi va cdng su cho biet bgnh hi chiem tdi 45% dta sd tai Viet Nam [4].Benh tti toy khtag de doa den su sing edn, nhung gSy khd chju, tah hudng din chit lugng cugc sdng ciia bgnh nhta. Mue tita chinh ciia dilu tti b$nh tti la gitei thilu cac ttita ehllng gay khd chju va cai thita chat lugng sing eho ngudi benh. Dilu
^ I f cd rit nhilu phucmg phap: Dilu chinh cbl dg ta udng, ehl do Itai viec, vg sinh tai ehd, dung thuic tota thta hoac tai ehd [1], cac thu thuat can thigp ngoai khoa (ttem xo, that vdng, khau ttiet mgch biii tti...).Y hgc eo ttTiyen ctog cd nhieu phuong phap dilu ttj nhu Sli dung thule thang sle uong, che tn, thuoc bdi, thule ngtoi tti...Chli ylu theo nguyen ly thanh nhi^t Igi thip, htah khi, boat huylt, luong huylt chi huylt, tieu viem tieu viem, gitai phu nl[l], [3].
Bai thule ATT la tta vilt tit ciia bai thule nghigm phuong dtog dl dilu ttj bgnh tn bfag phucmg phap ngam h|u mdn bao gdm cac vj thulo Hotag ba, Hotag eim, Xa stag hi, Dia du, Kinh gidi, Hde hoa, Nhu huong, Mgt duge cd tae dgng thanh nhiet lgi thip.
htah khi, hogt huylt, luong huylt chi huylt, tita vital. Muc tieu:
Bdnh gid kha nang gay kich iing hgu man cia ATT tren thuc nghiem H. CHAT LIEU, DOI TLTONG VA PHUONG PHAP NGHrtN ClTU
2.1. Nguyta lieu nghien ciru Tiianh phdn: Sta pham thule ngam iTi ATT bao gdm:
Hotag ba lOg Hotag elm lOg Hde hoa sao-vtag 20g Kinh gidi sao den lOg Dja du sao den 20g Xa stag tii 20g Mgt duge 5g Nhii huong 5g Thuic dugc xay tta thtah b^t, ddng gdi 50 g
Cdch diing: Cho thudc bgc vao ttii vai, dun soi vdi 01 lit nudc ttong vdng 10 phiit, sau dd ie ngudi va ngam tti, ngay 2 ifa, mdi ifa 20 phiit
2.2. Doi tirgrng nghita ciru Thd chtog Ne-w Zealand White, ca hai gidi, Idng frfag, trudng thanh, khde manh, eta ntag 2,0 - 2,2 kg do Trung ttal chto nudi dgng vjt thi nghiem Ha Tay cung cip. Thd duge nudi tti 1 ttifa fiTidc khi ttin hanh nghien ciiu va ttong suit thdi gian nghien ciru tai Eg mdn Dugc Iy, ttudng Dai hgc YHa Ngi.
Ngluen cdu YDHCT Viet Nam SSSO. 2016
2.3. Hoa chat, dung cu phgc vii nghita ciru
Nuoc muii smh ly NaCI 0,9%, catheter mim diu til, xylanh 1ml.
2.4. Phirong phap nghita ciru Nghien Cliu dugc tiln htah dua ttieo hudng dfa ciia ISO 10993 - 10 [7].
Quy trinh nghien cu-u tuifc tien hanh tren 6 Ihd nhusau:
Trudc khi bat diu nghien ciiu, dtah gia tmh ttang hta mdn ban diu:
tiet djch, sung nl, diu hita nhilm tttog, kich tog, tin thuong cCi....Loai bd nhimg thd cd bit thudng d hta mdn trgc tttag.
Thd duge chia thtah 2 Id:
• Ld 1 (n = 3): E)ua 1 ml nudc muoi sinh Iy vao hju mon - ttuc tttag
• Ld 2 (n = 3): Dua 1 ml thudc ngSm tti vao hta mdn - ttyc tttag
Thd d moi Id dugc dua nudc muii sinh Iy hojc thuoc thii vao hta mdn ttvc tttag ttong 5 ngay, moi ngay 1 Ita.
Bua thuoc vao hgu mon trifc trdng;
Hang ngay: Quan sat, danh gia hta mdn trgc tttag va viing day ehta
dl phat hita tmh ttang tilt dich, ban do va phu nl. Nhiing thd ed tinh ttang tiet dieh nhieu, phu ne nang vMiojc kho bom thudc vta se bj gilt va dtah gii md benh hgc. Cta thdi dilm dtah gia dai the (tilt djch, ban do, phi nl)
* 24h ttirdc khi dua ifa thuic diu tten
* Ngay trude mdi ifa dua thuic (ngay 1 dfa ngay 5)
* 24h sau lieu thude cuoi ctag.
Banh gid cuoi d0 nghiSn ciu:
Danh gia dgi thi vd ddnh gid mo bsnh figc:
Phta loai miic dO tin thuong mo btab hgc theo btag sau: bilu md, tham nhiem bjch ciu, sung huylt mach, phii HI. KET QUA NGHlfiN CiW
3.1. Danh gia dai thi hju mon trgc trang thd:
24 gid sau khi thd dugc dtag nudc mudi sinh ly hoae mfa thu ifa cuoi ctog, cac thd dugc mo dl phlu tich tota bO hju mdn tt%rc trtag.
Ve mjt dai thi, quan sat dugc Ial ca cac thd d 2 Id dfa khdng co hien tugng tin thucmg, hoai ta, niem mjc dai ttirc trtag khdng dd, khdng till djch, khdng phu.
Joumalof research in viet nam traditional medicine and pharmacy
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Hinh 3.1. Hinh anh h|u mon
true trtag tho Id 1 Hinh 3.2. Hinh anh h^u mon trirc tring thd Id 2 (dung thuic ngSm tri) 3.2. Danh gia ve mgt vi thi hju mon tnrc trang tho:
Lo 1: Dtog nudc mull sinh ly: (Kit qui hmh 3.3)
Tat ea thd dfa cd bita md phu niem mac, cac myfa cd sl lugng va hmh thai ttong gioi han bmh thudng, khdng mil ldp mtag nhiy, khdng thiy thoai hda hay hoai tu Md dem khdng phi, khdng hmh thtah cta d ap xe, md dem khdng ttag smh xo, lop CO khdng qua sta.
Hinh 3.3. Bilu md, m« dgm, m?ch mau binh thu*ng (HE x 200)
Nghien c&u YDHCT Viet Nam S6S0 - 2016
O tho 1 va tho 3, mo dem khong xSm nhap viem. Con tho 2, mo d6m co nai xam nhap viem nhe dSn viia. (K^t qua hmh 3.4)
Hinh 3.4. M6 dgm c6 noi x£m nh|p vi6m nhg din vira (HE x 200) Lo 2: Dtog thudc ngam tti ATT khdng pha lotag
Bita md phi ttgt nhg, khdng cd vung bao mdn, khdng thoai hda, di sta (Ket qua hinh 3.5)
Hinh 3.5. Bilu md phu trgt nhg * thd 16 2 (HE 1100)
Joumal of research in viet aam traditional medidne and pharmacy
Md dem phi cd xtoi nhap lan tda nhifa tl bta vital gim bach cfa da nhta ttmig tmh, Ijmipho bao, bach cau ai toan. Cta huylt quta khdng smig huylt, khdng gita rgng. (Ket qua hinh 3.6) = o / ^ s s
Hinh 3.6. Md dgm xam nhap tl bta viem d thd 16 2 (HE x 100) Btag dtah gia vi thi eho tting thd d eta Id nghita cuu
Stt 1 2 3 4 5
'
Bleu mo 0 0 0 1 1 1
ThSm nhiem b^ch cau 0 2 0 2 I 2
IU lung tno o cae 18 n Xung huyet mach
0 0 0 0 0
'-
ghien clhl Phu
0 0 0 1 1 1
Ting 0 2 0 4 3 4
Nghiin cOu YDHCT VietNam
Bang 3.2. Bang danh gia vi thi cho tCrag Id nghiSn cvu Lo
Ld 1: Nudc mudi sinh ly Ld 2: Thudc ngam tn
Tong diem vi the cia thd
2 11
Diem trung binh Id - Tong diem/ 3 thd
0,67 3,67
Chi so kich trng ciia 16
3 Tu ket qua d btag 3.1, 3.2 theo
phta loai muc do kich tog dua vta chi sl kich fag: Thule ngam Oi ATT gay kich fag rit nhe ddi vdi hau mdn true tttag thd. Tliulc ngam tti ATT khdng gjy ra cac tin thuang vi thi bilu md hju mdn trgc tttag thd.
rv. BAN LUAN
Hai Thugng Lta Ong da md ta vl bgnh tri va ding cac bai thudc nam dl chia bgnh. Btag khai nigm "tita huylt"
Tug Tihh neu nguyen nhta eia benh la do: Ham do chien xao, rugu che, dam dgc, lo nghT, uat nhigt tich dge. Bfag khai niem "Tri rd" dng da neu each chtta benh tti thanh: Tring tti va Kinh tti, moi logi cd nhihig bai thule nam phi hgp [3]. Cac bai thuoc: "Bl tnmg ich khi thang gia vj", "Hoe hoa tta",
"Tu vjt dao hong gia gitai" dugc cta y vta ndi din khi dilu tri tti diu cd chung nguyen Iy: thato nhi$t, Igi thip, luong huylt, ehi huylt [1], [3].
ATT la bai thude ngam ttt nghigm phuong, dua theo Iy Imin Y hgc cl truyen dtog ddi phap lap phuong co tta dung thanh nhidt lgi thip, htah khi, boat huyet, luong huylt chi huylt tieu vigm, chdng sung nl.
Theo qui dinh cia To chile y te thi gidi dtah cho thule cd ngudn gic tir dugc lita va Y hgc cl ttuyin: Trudc khi dua thudc ding tren Iam stag cin phai dtah gia tinh ta toan vd higu qua cia thude tten thgc nghiem [7]. Vdi thule ngam tti, thule tiep xue ttg:c tilp vta vtog tin thuong, tilp xiic niem mac ttvc tttag hju mdn nen danh gii tinh an toto cfa phai xac djnh kha nang gay kich fag hju mdn [7].
Danh gia kit qua nghien cfa theo cac ti6u tri tin thucmg dji thi vi vi thi diia vao cta chi sl kich fag nhu phi ni.
sung huylt, thjm tri la bien dli caa ttiic (nlu cd) [7]. Kit qua nghita cihl cho thit ATT khdng cd ton thuang |-
Jourmd ofreseard, In via nam Iraddtanal medidne andpharmacy
v . KET LUAN
No SO - 2016 khac biet gi rd ret khi danh gia dgi thi
va gay kich fag rit nhe niem mac hju mdn d ndng dg dam dac. Vi vay theo hutag dfa OECD, ATT an tota va cd the dtog dugc tt^n Iam stag cho ngudi [5], [6].
- ATT kich tog niem mac hju mdn true tttag mfa do rit nhg d ndng do djm dac.
- ATT an tota va cd thi dtog dugc trta Itai s t a g cho ngudi.
TAI LIEU THAM KHAO
L H o a n g Bao Chau (1999), Wphdp chira benh tri bang y hqc CO truyen", NXB Y hgc
2. Nguyen Trung Hgc (2009),
" & sanh kil qua.dieu tri phau thugt bfnh IrT theo hai phucmg phdp Longo va Milligan-Morgan lai benh vi^n Vi?t Btrc 2008-200r, Luin vta bac sy chuyta khoa II.
3. Hai Thugng Lta Ong (1997). Y long tdm lihh quyin I- Khon hoa thai chdn, nhiing tiiu trgng yiu cua hgu thien ty, vf. NXB Y hgc. tt 498- 0.510.
4. Nguyen Mjmh Nham (1999).
"Tinh hmh btah trl d mgt nha may (qua didu tta dich tl hgc va kit qua
budc diu c i a cdng tac dilu tti". Tgp chi Ngogi Ithoa, 4, tt. 15- 2 1 .
5. Trinh Hong Son va cs (2007).
"Phau thujt Longo dilu tti trT tic mgch" Tgp chi Y hgc Ihtrc hdnh sd 2/2007, tt 58- 60.
6. Nguyen Tat Tmng (2000).
Nghien ctru dieu tri benh tri bdng thu thugt thdt trl cdi liin kil hpp tiem thuoc kho trt ctia y hgc c6 truyin.
Luta ta Tiln s i y hgc, Tnrdng Dai hgc Y H a N g i , t t . 94,108.
7. Intemationai Organisation of StandardizaHon (2010), ••Biological evaluation of medical devices - Part 10: Tests for initation and skin sensitization", ISO 10993-10.
