XAC DINH THANH PHAN H O A HQC CUA TINH DAU CAY IHTONG NHU TRANG CS NGH|: AN
Nguyin Th! Chung'
TOM TAX
Tinh dSu la cay hiicrag nhu trang {ocimum gratissmum L.) a Thanh Chuong, Ngh? An dugc tach hing phuang phap chung ck 16i cu6n hoi nudc. H ^ luQUg tinfa d ^ thu dugc la 0,5% so vdi nguyen lieu tuoi dugc phan tich bang phuang phap-sdc k^ khi (GC) vk sac ky khi - kh6i ph6 ky lien hgp (GC/MS). Co 29 hgp chit da dugc x&c d|nh chigm hem 96,42% tdng ham lugng tinh diu. Thanh phSn h6a hgc cua tinh diu chii ylu 14 cac hgp chit monotecpen va secquitecpen trong do thinh phSn chinh la eugenol 74,56% ; gecmacren - D 6,59% va (E) - p - ocimen 5,38%.
Tit kh6a: Tinh dau cay hucmg nhu, hgp chat eugenol.
ABSTRACT
The leaf oil of ocimum gratissmum L. collected from Nghe An was isolated by steam distillation to give oil yield 0.5% and analyzed by capillary GC and GC/MS. 29 components have been identified accounting more than 96,42% of the oil respectively. The main components of ihe oil are mainly compounds of the type monotecpen and secquitecpen including eugenol 74,56%; gecmacren - D 6,59% and(E) -/3- ocimen 5,38%.
Key words: Ocimum essential oil, eugenol compound.
I. DAT VAN DE
Cay hucmg nhu trang {ocimum gratissmum L.) thu^c hg hoa moi (Lahiatae) mpc hoang d nhi6u vung cua nudc ta. Theo nghien cdu cua cac nha th\rc saX. hgc thi cSy huong nhu tring co nguon g6c d vimg nhiet ddi Chau Phi hoac An Dg. Ngay nay, cay dugc tihag a mgt s6 nudc khac nhu vimg Trung, Nam Phi va Dong Nam A, trong dd c6 duy nhit Vi^
Nam da trong huoug nhu trang tren pham vi rong d nhi6u dia phucmg. Trong dan gian, UM.
cay nay dugc dung lam thu6c dk ha s6t, chua cam nhit la cim ning, say ning, nhdc diu, dau b ^ g , di ngoai, non mira, phu thiing, hoi mi?ng [1].
Tren the gidi, ciing nhu d Viet Nam ngudi ta da phat hi?n ph§n tren m|t dit huong nhiiv^
tiing chlia nhieu tinh diu va da phan thanh phin tinh diu trong cay thanh nhidu nh6m hod hpc ' (Chemotype) nhu: nhom giau eugenol, nhom giau p-caryophylen (39,5%) vk gennacrai (29,9%), nhom giau thymol [1,5].
' ThS. TnionE Dai hoc Vinh..
J
r •'
T/?PCHfKHOAHOCS66
Ding cW J? l i loii hmmg nhu cho tinh din chtra nhidu eugenol se rit cd gii tri hong thvc ndn. Eugenol chid, xnit «t tinh tiiu duac dting tong nha khoa va l i nguyen lifu de tong h ^ chi. Atom vanilm Tu «ngenoI teng hop ndn mCyl eugenol dung Iim chi. din dv ruo, ving h , , cam. Tmh dau huong nhu cdn cd did tttay ftd cho tinh diu dinh huong tong cac nganh sin xua. hum,g lieu, x i phdng va y du,c. Bac biet chd phim tttude phd b,en nha. cna tinh dau cay huong nhu .ring la diu cao "Sao vang" m i chung m ttiudng
dung cmtg nhu de xuit khau[I,4,5]. ^ Trong bii bio niy, chung tdi gop phin cmig cip din lieu vd ttianh phin hda hoc
tinh dau cay huong nhu tiing a Nghd An nhim bd smig tt,em eac dtt kien vd mat hda hoe tinh dau cay huang nhu trang.
n . THirC NGHIfM 2.1. Thilt bi
viu, , , ^ t t ^ . * ° ? * " ' ' * * " " ^ " ' - " * ' ' ' ' « " = ' " Technologies HP 6890N Plus gin T H I l ^ r - ' ^«"™',T-hnologies. My. s i c ky khi-khdi phd (GC/MS) d u ^ T V , ? i ' " ' " " " " ^ " ^ ^ '' ""^ "^ " » " ' P °C/MS cua h&ng Agilent Technologies HP 6890N. Agilem Technologies HP 6890N/ HP 5973 MSD duo! lip vdt cpt tach mao quin vi vin hinh vdi He lim khi mang.
2 J . Nguydn lifu thnc v^t
duoc b o " ' " ^ """"'T ' ^ ' * " " * " " " * ™ " * ^ " " " " S . Nghe An. Mlu tttuc vat duac bo mon Tlvrc vat. khoa Sinh. Trutag Dai hoc Vinh xac dinh la humig nhutring (oc,mt«H^a,„s„„„L.) thupc ho hoa mdi (Lahiatae).
23. Tich, xic ajnh thinh p h i n hda hpc tinh d^u
nudc iron "^V • ' " " ' f ' ' . * " * ' "' " ' ™ ^ ' ' • " ^ ^ P " ™ * P^O ^ " ' " S "^^ ' « cu^n hoi nudc tong tti,^ gian 3 gto o ap suit ttiutag ttieo tieu chuin Duac didn Viet Nam I [2]
H t a Itrong tinh dau li tinh ttteo nguyen li^u tiroi la 0,5%. Tinh diu thu duac cd mau v L g
^ ^ ^ l r X ' " " " " " * ' '^'- ' " ' = ' * " ' * " ^ ^ 8 Na.SO. khan, bao quan 6 nhiftdStirO-5 "C tiirdc khi phan ti'ch.
oh^n n f \ T - , l : ' T ^ " ' " '"'°« ' " ^ ™ ' ^ ' " " • " ^ ^ ^^'^ • * hoac loai dung eho
Cmn), a n g 4 C/mm cho den 220"C, dtog o nhiet dd nay tong 10 min. Nhiet dd budng bom mlu 250''C. Nhift dp detecto 260°C, Ml, mang la He. iNmet do buong
^ ' ^ ^ f = ° * cic eiu t i dupc ttiuc hien bing each so sanh eac du Men phd MS eua
* m g v . h p h 6 c h * didupc Cdng bdcd tong tt,u vien Willey/Chemstation HP T ^ g r n d t ^ mrtaghpp^tadmtiabangeiechattiangtinhdindabidthoacchitchuinpdl
m . KfiT QUA VA THAO LUAN
dupcc.^Sss"!:!^'*'""'""'""^'"*'^-^^^'-"™^'*"^^^'^'^^^'^
6
Bing 1. Tyli%
TT I 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Hop chit a - thujen a - pinen sabinen 3 - myrxen a - teepinen cymen (E)- 3- ocimen y-teepinen Cis- sabinen hidrat Octanol-I a- tecpinolen Linalol alio - ocimen Teepinen - 4- ol Eugenol
TRUONGOAI HtJC HA TUB!
cdc chdt cd trong tinh ddu cdy huang nhu trdi^ d Ng^An - H i m lurpng %
0,23 0,03 0.26 0,19 0,07 0.03 5 3 8 0.14 0.27 0.05 0.03 0.12 3,68 0.24 74,56
TT 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Hlfp c h i t a - copaen 3 - buorbonen 3 -cubeben 3 - elemen bieycio elemen a-cubeben 3- caryophylen a- amorphen a- humulen germacren- D 5- cadinen Y- muttrolen caryophylen oxit a- cadinol Cic hpp chat khic
H i m l i i ^ g % ' 0.59 0.24 0.59 0.24 0.02 0,02 2.24 0,03 0,10 6,59 0,18 0.19 0.08 0,03 1 3,56 Theo ket qui phin ti'ch cho thiy ttiinh phin hoa hpc cua tittt, diu cay hucmg nhu tring d Thanh Chuang, Nghe An da xic dinh dupc 29 hpp chi. chiim 96.42% , Thinh phin chinh cua tinh diu la eugenol voi him lupng Ion (74,56%). MOt s6 hpp chit khac cd him lupng tirong ddi cao nhu germacren - D (6,59%), (E) - p - ocunen (5,38%), aUo - ocimen (3,68%),
Eugenol li hpp chit cd gia tr. thuc tidn cao. cdng thtic phan tiJt cua eugenol 14 C10H12O2 (cd tte dung diet khuin. dimg chtta dau nhic rang tong nha khoa). Tiir eugenol ngudi ta didu chd meQileugenoI (chi. din du rudi ving hai cam), axi. eugenoxiaxetic (chi.
kich ttuch h j . ngu cdc nay mim) va vanilin (huong lieu cho banh k?o).
Tit y nghia thirc tien, cay huong nhu tting duac tidng tidn di$n rOng di sin xui.
tinh diu dimg tiong nutjc va xui. khiu. Didu dang quan tam l i cac mlu tinh diu ttiu ttijlp d nhidu dia phuang khac nhau cua Viet Nam ddu cho ttiiy tinh diu cay huong nhu tting d ntrdc ta cd ham lupng eugenol cao cd ttid dat tdd 74-80%[I,5]. Nhu vay. loii huong nhu tting Uiupc ddi tepng nghien cttu o tten cung dupc xdp vio chemotype Eugenol r i . cd gii ni, gdp phan cung cip din lieu vd ttianh phin hda hpc tinh diu ciy huong nhu tting cting nhu vide didu tta tidm ning ngudn tii nguyen tinh diu.
IV, KET LUAN
I. Da lay man, tach va xac dinh h t o lupng tinh diu ciy huong nhu tting d Thanh Chuong, Nghj An. Cic kit qua phan tich cho tttiy h t o lupng tinh dau l i 0.5% ttieo him lupng tiroi.
TAPCHfKHBAHOCs66
2. BSng phuong phip sac ky khi vi sic ky khi - khdi phd ky hen hpp da xic dmh dirpc fliinh phan hoi hpc cua tinh din cay huong nhu tiing 6 Nghd An chu ydu la monotecpen va secquitecpen vtSi ttiinh phiu chinh Ii eugenol chidm ddn 74.56% Diy Ii mO. tttmg riiung hap chi. cd gii tti tong cdng nghiep chd bidn huong lidu, my phim dupe ptan vi ndng nghidp. Ngoii ta mlu cdn cd nhung hpp chit khac eo hto lupng tirong dot cao nhu germacren-D (6.59%). (E)-p-acimen (5.38%), allo-ocmien (3 68%)
3, Loa, huong nhu tting a Thanh Chuang, Nghe An dupc xdp vio chemotype Eugenol ra. CO gm.n.
TAI LIJU THAM KHAO
Khoahi'ia^^ to^'* "' ' ™ " ' ' ° ° " ' '^^ "•"'' • " * " « "* '""• ""'•=' ''*«'™. NXB [2]. Dugc diin ViflNam I (1971), NXB Y hpc
p h a m ^ J ^ o e ^ * " ' ' ' ° " ' ™ ' ^ " ^ ^ ^ ^ < ™ " - ^ ' ^ - " • ' " ' •^'^ Purification of eugenol Britieh Abo,,, }^'^^°^ ^"™ °™^' ^-^^ ^ ' c ' ^ ' C ' ^ ° ^ Moi, Nguyen Thi Phuong Thao fl987,
data. w f S . N : : f S . ' - ' " " * " " " ° '•• ' * ^ ^ * ' ^ "• * " " ^ ) - ''^^-'^ " Z " - ^^-'^•"
Ngiy nhin bii bio: 31/7/2015 Ngiy phin bifn long: 1/9/2015
