NGHIEN Cliu TAG DUNG CHONG OXY HOA CUA CAC CAY GOI HAC LEEARUBRA^UMl
NGUYEN DUY THUAN, N G U Y I N THE THINH, PHAM QUOC BINH Hgc Vidn YDugfc co truyen ViStNam N G U Y I N T H I E N T H U O N G , Vien Duw: lidu
L§ ANH TUAN, Bdnh vi^n YHCT Hd Ddng
T6M TAT
R§ cdy Gdi h^c vd phdn doan EtOAc. n-BuOH cd tdc dung dgn cdc gdc DPPH. OH* va 02-* Trong dd kit qud qudt gdc t y do DPPH tdt han cd vd khd ndng qudt gdc tt/ do m^nh nhit duge thi hidn d phdn do$n n-BuOH cdc gid trj IC50 cua cdc phan doan Iin /upi Id 10.1; 8.6 va 5.8 (pg/ml). Gid tq nay cua chit ddi chOrtg duang catechin Id 2.5 (pg/ml). Nhw vdy tdc dicing qudt gdc ti/ do cOa djch chiit todn phdn va phin doan EtOAc. n-BuOH Id tuang ddi manh. Kdt qud nghidn ciru tdc dung loai bd goc tu do cOa Gdi h$c c6 thi g6p phin giii thich ca chd tdc dung chong vidm cOa cdy thudc, vd ggi y cho vi6c phdt trien khai thdc cdy thudc theo hudng ben vt/ng va hidn dai
Tip khda: Gdi h$c, Leea rubra Blume SUMMARY
The roots of Goi hac (Leea rubra Blume) and fractional EtOAc, n-BuOH wori< clearing DPPH radicals, OH and 02-: V\^ich results in free radical scavenger DPPH better and the ability to scan the most powertui free radical Is shown in segment n- BuOH IC50 values of 10.1 segments respectively; 8.6 and 5 8 (g / ml). This value of the positive control substance catechin was 2 5 (g/ml). Such free radical scavenger effects of extracts and whole segments of EtOAc, n-BuOH is relatively strong Results of the study effect of removing free radicals may contribute explain anti-inflammatory mechanism of action of medicinal plants, and suggestions for the development of exploitation of medicinal plants In a sustainable way and modem
Keywords: Goi hac, Leea rubra Blume OAT V A N D £
Y hpe d truydn nud'c ta cd tir ngdn ddi, ton tai va phat tridn song song ciing vdi ndn Y hgc phuang Ddng. Vdi nh&ng phuang phdp didu tn dfie dao, dua trdn ca sd cua mfit hd thdng ly luan chdt che, bien ChCrng logic vd mfit kho tang kinh nghidm chO'a bdnh trong ddn gian, dd vd dang mang Igi hidu qua cao khi didu trj nhidu chO-ng benh, ke ca nhu'ng bdnh nan y va man tinh.
NhO'ng thdp ky g i n day, edc nha khoa hpc Y Duge trong nude cOng nhu tren Thd gidi quan nhidu tdi vide nghidn cO'u cdc cdy thude, vj thudc tu' thidn nhidn, c6 tac dung tot dd ung dyng trong didu tri bdnh, dde bidt la nhCrng bdnh ly hay gap hidn nay, cd lidn quan tdi qud trinh oxy hod trong ca thd nhu:
bdnh tim mgch, ung thu, Pari<inson, Alzheimer, tidu
dudng, qua trinh vidm d nhidu bdnh ly trong dd ed cdc bdnh ve khdp (vidm khfi'p dang thdp, thodi hoa khdp...)[1].
Viet Nam cd ngudn tai nguydn vd duge lieu khd phong phu, theo udc tinh Y hgc ddn gian da phdt hidn khoang 4000 cay thudc trong sd khodng 12000 loai thuc vdt duge biet [2], [3]. Tuy nhidn cac nghidn eCru de khai thdc sCr dung chCing mfit each khoa hgc, hi$u qua hidn eon r i t khidm tdn Trong giai doan 2010 d i n nay, chung tdi da thuc hien d l tdi "Nghien cuu sdng Igc cdc cdy thudc Wet Nam c6 tdc dung chong oxy hda di phdn Idp cdc ho^t chit cd tdc dijng phdng vd diiu tri cdc bdnh tim mach" do Quy phdt tridn khoa hgc va cdng ngtie Qude gia (NAFOSTED) tdi trg.
Trong bai bdo ndy, chdng tfii thfing bao k i t qud.
"Nghidn CIFU tdc dung chdng oxy hod cua cdy gdi hac (Leea rubra Blume.)"
D 6 | T U - O N G , NGUYEN LIEU, PHU'ONG P H A P NGHIEN CLTU
1. Nguyen li^u 1.1. DoitwoTig
- Cdy goi hac (Leea rubra Blume.) thu hdi tai vudn bao tdn cay thude d huydn Nam Ddng, thufic V u d n Quoc gia Bach Md tinh ThCra Thleji Hue.
- Nguyen hdu nghidn eCru la rd cdy Goi hgc duge thu hdl vao thang 8/2012, sau khi rira sach 6ifgc s l y d 60°C, den khd; xay thdnh bdt thd.
1.2. Nguy§n vdt li^u hda chat
Cac dung mdi, hda c h i t c i n thidt ddu dat tidu chudn eho thir tdc dung sinh hoc, 6iegc mua eua eac hang Merck, Sigma-Aldrieh, Invitrogen .. gdm ed'
- Gdc t u do DPPH (Sigma),
- Cdc hda c h i t pha dung dich ddm: K2HP04 (hodc KH2P04), NaOH, HCI, ethytenediaminetetraacetic acid (EDTA)
- Cdc c h i t duge sCr dung dd Idm ea c h i t hodc tac nhan oxy hda: xanthin, nitro blue tetrazolium (NBT), (EDTA), ascorbic acid (vitamin C), FeCI3, dufi'ng 2- deoxynbose (Sigma), TBA, TCA.
- Cdc chdt doi chidu. (+)-eatechin.
2. Dyng cy mdy mdc - Mdy do pH
- Ong ly tdm 1,5 vd ddu cdn cae logi - DTa 96 gidng
- Mdy do quang UV - VIS, do quang ELISA Multiskan cua Thermo.
- Tu I m (37''C)
- Pipet- dOng pipel cdc cfi' (0.5MI -100.0 pi)
Y HOC THIJC HANH (940) - s 6 11/2014
- Ong nghidm 2 ml.
3. Dja didm nghien c i r u : Vien nghidn cCru Y Duge ed toiydn Tud TTnh - Hgc vidn Y Duge hgc cd truydn Vidt Nam vd Vidn Duge lidu.
4. Phuang phdp nghien ciru 4.1. Chuin bf mSu di sdng Igc
Quy trinh chidt nhu sau: Chidt xult bdt duge lidu b l n g MeOH (chidt ndng). Cfi djch chidt, thu duge c i n khfi. Hda eIn trong nude de duge hdn djch rdi dem l i e vdi dung mdi n-Hexan (Hx). pi ydn eho Idp Hx phdn dogn rdi gan l l y djch Hx. L i e vdi dieh Hx nhidu l l n ddn khi lap Hx nhat mau. Gdp cdc dich Hx, cfi cgn, thu duge e I n phdn doan Hx. Tidp tyc lam nhu vdy vdi cae dung mfii ethyl acetate (EtOAc), buthanol (n-BuOH), se thu 6iegc cac phdn dogn EtOAc, n- BuOH va eIn HjO.
C i n H^O va c i n cac phan dogn n-Hexan, EtOAc, n-BuOH duac hda tan trong MeOH d cdc ndng dfi duac ehgn de sdng loc hoat tinh chdng oxy hda,
4.2. Phuxmg phdp thv tdc dung dgn goc tw do DPPH [4], [5], [6].
* Tien hanh thi nghidm.
- Pha mdu thu trong MeOH thdnh dung djch, ed ndng dd chinh xdc khde nhau _
- Cho vdo dng nghidm hdn hgp gdm cd: 10 pi dung dich m l u thO-, 490 pi dung dich DPPH da pha.
Trfin hdn hgp trong khodng 1 phCit rdi dd ydn 30 phut trong bdng tdi, d nhidt dfi thudng Dung djch sau khi phan Crng duge dem do mat dfi quang (A) d budc sdng 517 nm. Tidn hdnh do mat dd quang cua m l u cung vdi m l u tring (500 pi dung dieh DPPH), dung dieh c h i t thir trong 500 pi MeOH vd m l u ddi chung duang (10 pi dung dich c h i t ddi chidu, 490 pi dung djch DPPH) [161.
* Tinh kdt qua
Kha nang dgn gdc t u do eua mau thu (cho ed c h i t ddi chidu duang) duge tinh theo cdng thCrc:
HT(%) = [1 - ( A m t - A m ) / A O ] x 1 0 0 Trong dfi'
- HT (%). Kha ndng dgn goc t u do DPPH.
- Ao. Do h i p thy dnh sang eua dung dieh DPPH khdng cho mdu thCr.
- Am: Dfi hdp thu anh sdng cua dung djch DPPH cfi eho m l u thir.
- Amt: Dfi h i p thy dnh sdng eua dung dich dem ehi cho mluthCr.
4.3. Phuxyng phdp thw tdc dung dgn gdc t{p do superoxide anion (O'") [4], [5], [6]
* Tidn hanh thi nghidm
- Pha cdc dung dieh ddm phosphat tCr hfin hgp KjHPO., (hodc KH2PO4) dd ed duac dung djch d ndng dfi 20 mM ehua 0.1 mM EDTA vd cd dfi pH 7.8;
dung dich xanthin 5 mM, dung dich nitro blue tetrazolium (NBT) d ndng do 5 mM vd dung dieh enzym xanthin oxidase (XO) (pha lodng trong nude tinh khiet) d ndng dfi khoang 2 mM
- Cho vao dng nghidm hdn hgp gdm cd: 10 pi dung djch mdu thi>, 460 ^1 dung dich ddm phosphat, 10 pi dung djch xanthin 5 mM, 10 fjl dung dich NBT 5
mM va cudi ciing cho 10 pi dung djch enzyni XO.
Trdn ddu hSn hgp trong khoang 1 phut rdi 6& ydn trong 5 phut tidp theo d ^ e u kidn thudng Dung djch thu duac dem do mdt dd quang (Am) d budc sdng 560 nm. Tidn hdnh do mdt do quang cua mdu cCing vdi m l u trdng dd so sanh (khdng cd m l u t_hu vi khdng eho enzym XO), dung dich chi cho m l u thCr (Amt 10 pi dung dieh m l u trong 490 pi dung dich dem), dung djch ptian ung khdng eho m l u (Ao) va m l u ddi Chung duang.
* Tinh kdt qua
Kha ndng dgn gdc t u do O^^ cua m l u thO- (cho ca c h i t ddi chidu duang) duge tinh theo cdng thCrc.
HT (%) = [1 - (Amt - Am)/A0]x100 Trong dd:
- HT (%): Khd ndng dgn gdc t u do O^"
- Ao' Dfi hdp thy dnh sdng eua dung djch 0^' khfing eho mdu thu
- Am' Dfi hdp thy dnh sang eua dung djch O^" co cho m l u thCr.
- Amt: Dfi hdp thy anh sang cua dung djch d$m chi cho m l u thu
4.4. Phuxmg phdp thw tac dung dgn goc tw do hydroxyl radical (OH'). [4], [5], [6]
* Tien hanh thi nghidm
- Pha m l u thCr trong MeOH thdnh dung djch, c6 ndng dd chinh xdc khae nhau._
- Cho vao dng nghiem hdn hgp gdm ed: 10 \i\
dung dich m l u thCr, 470pl dung dich ddm phosphat, 10 pi dung dich dudng deoxyribose vd lOpI dung dieh Fe^*. Trfin hdn hgp trong khoang 1 phut rdi de yen trong 30 phCit a bdng tdi, nhidt dd thudng. Dung djch sau khi phan Crng duge cho thdm 500 pi dung dich TCA 20% vd 500 pi dung djch TBA 1%, sau d6 dem dun sdi cdch thuy trong khoang 15 phCit. H5n hgp thu duge dd ngufii ddn nhidt dd thudng rdi dem do mat dd quang (Am) 6' budc sdng 532 nm, Tidn hdnh do mat dfi quang eua mau cOng vdi m l u tring de so sanh (khdng cd c h i t thCr vd khdng eho F e ^ , dung djch c h i t thCr trong dung dich ddm (AmtlO pi dung djch m l u thu. 490_MI dung dich ddm), dung djch phan Crng khdng cho mau thii' (Ao: 480 pi dung dicii dem phosphat, 10 yl dung dieh dudng deoxyribose, 10 pi dung dich Fe^*) va mdu ddi chCrng duang.
* Tinh kdt q u i :
Kha nang dgn gdc t y do OH- cua m l u thir (cho ca c h i t ddi chidu duang) duac tinh theo cdng thO'c:
HT (%) = [1 - (Amt - Am)/A01xi 00.
Trong dd:
- HT (%): Khd ndng dgn gdc t y do OH*
- Ao: Dd h i p thu dnh sdng eOa dung dich OH- khdng cho m l u .
- Am- Dd hdp thu dnh sang cua dung dich OH* c6 cho mdu thir.
- Amt:_ed d p thu dnh sdng cua dung dich ddm chi cho mdu thCr
K^T QUA NGHIEN CU'U VA B A N L U A N 1. Kit qud sdng Igc DPPH cua ri cSy thudc g6i hac & nong d^ khic nhau/MeOH
Y HOC THirc HANH (940) -
B l n g 1. Tac dyng logi bd gdc t u do DPPH eua cao gdi hac
% logi bo g6c DPPH 100 j 50 1 25 12.5 I 6 25 I IC50 (Mg/ml) (ug/mi) (ug/mt) (ug/ml) (ug/mi) ^jg/ml nAihar- 96,30 j 97,12 97,25 73,46 41,1
^^•^^'^ I ±1,39 ±0,60 ±1,18 5,0 Nhdn xdt. Kdt qua sdng Ipe cho thiy cdy thudc Gdi hgc ed tdc dyng dpn gdc t y do DPPH tdt, vdi gid tn tC50 = 5 0 yg/ml. TCre Id vi thudc Goi hac ed tde dung tdt vdi gia trj IC50 = 5 0 yg/ml. Gdi hgc la v| thudc, da duge sir dung rfing rai trong ddn gian 6k chu'a cac chung bdnti phong thap, tidu vidm. Ket qud sdng Igc da ggi y ring, cd thd ed mdi lldn quan giira tdc dyng loai bd gdc t y do cua cdy thude vdi tdc dyng chdng vidm. Vi vdy, nghidn cCru tdc dyng loai bd gdc t u do cua Gdi hgc cd thd gdp p h i n gidi thich ca chd tae dyng cua cay thudc, va ggi y cho vide phat tridn khai thdc cdy thuoc theo hud'ng bdn yCrng va hidn dgi.
2. Kdt qua vd tdc dung chdng oxy hod ciia djch chidt vd cdc phdn doan tO" rd cdy Gdi hac
Tdc dyng dgn gdc t y do eiJa eao djch chidt todn phdn va cac phdn dogn n-Hexan, EtOAc, n-BuOH, Cin nude cua r l cdy Gdi hgc duge thCr theo eac phuang phdp da md ta, ket qud duge trinh bdy trong cac(Bang2, 3, 4),
2.1. Tdc diing loai bd gdc tw do DPPH Bang 2:Tdc dyng loai bd gdc t y do DPPH ciia cac phdn dogn
Vdi catechin IC50 = 2 5 yg/ml
Nhan xdt: K i t qua eho thdy cao todn p h i n vd tat ca edc phan dogn cua djch chidt tir re cdy Gdi hgc ddu cd tdc dyng logi bd gdc t y do DPPH, tdc dyng mgnh nhdt thd hidn d cac phan doan n-BuOH, EtOAc va eao toan p h i n .
2.2. Tdc d{ing lo^i bd goc tw do OH- Bang 3- Tac dyng loai bd gdc t y do OH- cOa eac 3han doan
MAU THCT T, phin Hexan EtOAc n- BuOH
C<n nifdc
HOAT TINH%
2,5 (Mg/mll
10 9 9 10 0
6 (ug/ml)
19.1 9 16,7 15.8 S
10 (MS/ml)
25 10 24 26,1 12,5
20 (pg/ml)
34,8 14,7 35 40 20,1
50 (ng/mi)
56.1 25,6 59,4 62.1 30
IC50 42,1
>100 33,6 31,2
>100
V d i catechin IC50 = 4 4 yg/ml
Nhan xet: Kdt qud cho t h i y eao todn p h i n va cac phan doan EtOAc, n-BuOH cung cfi tdc dung logi bd gdc t y do O H -
2.3. Tdc dung loai bo goc t{r do O^"
Bang 4. Tdc dyng loai bd gdc t u do O^" cua cdc phan doan
M A U TH1>
Todn ph^n n- Hexan EtOAc n- BuOH
Cin nLf6^
HOAT TINH%
2,6 (Mg/ml)
9 0 10 8 5
6 (ug/mi)
15,6 9 16,6 13,5 8
10 (pg/ml)
22,3 15,7 26 27 12
20 (ua'mi)
37.5 21,3 38,1 40 16,6
60 60,1 30,1 63 67.8 25.9
IC50 36.0
»100 31.2 26.1
>100
MAu THl>
Toin phdn n- Hexan EtOAc BuOH Cin nu-dc
H0ATTINH%
25 (Ug/ml)
12,2 5 16 19 7,9
5 (ug/ml)
26 8.5 30 47 9
10 (Mg/ml)
401 11 46.5 66,1 13,5
20 (ug/ml)
67,9 21,1 90,1 100 20
50 (ug/ml)
.100 50,9 100 100 56,1
IC50 (ug/ml)
10,1
>20 86 5,8
>20
Vdi catechin IC50 = 3,6 yg/ml
Nhdn xdt- Tdc dyng loai bd gdc t y do O^" duge thd hidn d cdc phdn doan n-BuOH, EtOAc vd eao toan p h i n , phdn dogn n-BuOH eho tdc dyng tdt n h i t
Tir kdt qua (Blng 2; 3; 4) d trdn cho thdy cao djch chidt tir rd cdy Gdi hac vd phdn dogn EtOAc, n-BuOH ddu cho tdc dyng dpn cae gdc DPPH, OH* va O^".
Trong dfi kdt qud qudt goc t u do DPPH (Bang 2) xem nhu tdt han cd vd kha ndng qudt gdc t y do mgnh nhit duge the hidn a phdn doan n-BuOH cdc gid trj IC50 eua eac phan dogn l l n lugt la 10.1; 8.6 vd 5.8 (yg/ml).
Gia tn nay eua chdt ddt chu'ng duang catechin ta 2.5 (pg/ml). Nhu vay tdc dung qudt gdc t u do cOa djch chidt todn p h i n vd phdn dogn EtOAc, n-BuOH Id tuang ddi manh. D i l u dd ed thd khIng dinh ring trong ba mau trdn c h i c c h i n cd chii'a cdc chdt chdng oxy hda vd chu'a nhidu nhdt d phan dogn n-BuOH.
K£T LUAN
* Da sang Igc tdc dyng loai bd gdc t y do DPPH eua cdy thudc gdi hgc (Leea rubra Blume.). Cho kdt qua tdt, vdi gia tri IC50 < 100 yg/ml
* Da danh gid tdc dyng chdng oxy hda cua cCia cdc phan doan dich chidt tCr rd cdy Gdi hac
Tdc dung tdt nhdt a cdc phan doan n-BuOH EtOAc
+ Cdc phdn 6o$n cho tdc dijng tit trong thi nghidm loai bd gdc tt/ do DPPH
- Phdn dogn EtOAc ed IC50 = 8,6 ± 0,61 pg/ml.
- Phdn dogn n-BuOH cd 1C50 = 5,8 ± 0,25 yg/ml.
+ Cdc phdn 6o$n cho tdc dijng tdt trong thi nghidm loai bd gdc ti/ do OH'
- Phan doan EtOAc cd IC50= 38,6 ± 0,43 yg/ml.
- Phdn doan n-BuOH cd IC50 = 31,2 ± 0,43 pg/ml.
+ Cdc phdn 6oan cho tdc d^ng tdt trong thi nghidm bd goc ti/ do O^"
Phdn dogn EtOAc cd IC50 = 26,1± 0,83 pg/ml.
- Phdn doan n-BuOH ed IC50= 31,2±1,01 pg/ml.
TAI LIEU THAM K H A O
1. Halliwell, B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence.
Lancet 1994, 344, 721-724,
Y HOC THUC HANH (940) - S6 11/2014
2 Nguyen Duy Thudn vd Cs (20051, Tdl nguydn cdy thuoc Viet Nam. Trong: Bdo cao tdng kdt de tdi e l p nha nude (KC 1007) "Danh gia vd nghidn cCru, dd xult giai phdp ddng bfi de phat trien bdn vO'ng ngudn tai nguyen duac lidu Viet Nam", 10/2005,
3. Pham Hodng Hfi (2000), Thuc v$t Vidt Nam.
Nha xult ban tre, Thdnh pho Ho Chi Mmh, 4. Nguyin Thi Thu Huang (2006), Phuang phdp nghidn cdu duac II thudc c6 tdc dung chdng oxy hda.
Trong eudn "Phuang phdp nghidn cuv tdc dung duac
II cOa thudc td dum: thdo". Vidn Duac ligu, Bfi Y td.
NXB Khoa hgc Ky thugt,
5 Thuong P.T.; Na M,K ; Dang, N.H.; Hung T.M.;
Ky P.T.; Thanh T.V.; Nam N H . , Thuan N.D.; Sok D.E.; Bae K.H.(2006), Antioxidant activities of Vietnamese medicinal plants Natural Pmduct Sciences, 12, 29-37,
6. Blois, M.S. Antioxidant detenninations by the use of a stable free radical. Nature 1958, 181. 1199- 1200.
KHAO SAT SUROI LOAN LIPID MAU
Of BENH NHAN TANG HUYET AP DIEU TRI TAI BENH VIEN NGO HANH SON
MAI HCTU PHUOC TOM T A T
Miu n = 95 BN tdng huydt dp (THA) diiu tri t$i Bdnh vi$n Ngu Hdnh San, td 10/2013 - 9/2014. Muc tidu: Khdo sdt roi lo$n lipid d benh nhdn tdng huyet dp didu trj tai BV Ngu Hdnh San.
Kitqud: TiinsdTHA- 81,1%, khdng biit tien sd RL lipid mdu 100%, BHYT 100%.
D$c didm LS: Tudi tmng binh: 65,58 ± 12,20. can ndng tmng binh: 51.69 ± 8,19 (kg) (nam: 54,63 ± 6,6;
nd 50.25 ± 8.49); rdi loan lipid mdu: 71,6%. p < 0.01 (nam: 66,7%; nCr: 73.9%). Ngdy ndm vidn tmng binh:
8,29 ± 4,34. trung vi (median) va mode Id7,p< 0,01;
THA tdm thu- 98,9%, THA tam tnrang: 70,5%, THA tdm thu va tdm tmxyng: 69,5%, p < 0,01; THA giai doan 1:33,7% vd giai 6o$n II: 66,3%, p < 0,01.
D0C diim CLS' TSng cholesterol mdu: 41.1%, p >
0.05; tdng tnglycende mdu: 37,9%. p < 0,01; giam HDL-C mdu: 42.1%, p > 0.05;. tdng LDL-c mdu:
25.3%, p < 0,01, rd/ loan 1-2 thdnh phin lipid mdu:
50.5%, p < 0.01; ECG thudng quy cho kit qud bit thudng: 70.5%. p < 0.01.
Cdc mdi tuong quan. Tuang quan thu$n yiu giO'a cholestemi va cdn n^ng, vdi r= 0,15; p> 0,05; tuang quan thuan manh giO'a cholesterol vd LDL-c, vdi r = 0,88. p < 0,01; tuong quan nghjch gida HDL-c vd LDL-c, vdir=-0.28; p< 0,01.
Di nghi: Tdng eudng tuyen tmydn, thiit lap di/dng ddy tw vin, triin khai cdc dan vj khdm phdt hidn, (ftiu tri, qudn ly bdnh nhdn THA cd RL lipid mdu. Diiu trj RL lipid mdu Id quan trgng dd phdng trdnh bdnh m^ch vdnh trong tuong lai.
Td-khda: Rdi lo^n lipid, tdng huydt dp SUMMARY
Dyslipldemia in the patients with hyperteslon at the Internal Medicine Department, Ngu Hanh Son hospital, Da Nang city, Viet Nam
Introduction: We studied the lipid level of 95 patients with hypertension (HTN) who were admitted to the Intemal Medicine Department of Ngu Hanh Son hospital from October, 2013 to September, 2014
Aims: To assess the clinical characteristics, laboratory findings and their correlations in order to design a preventive strategy for patients with dyslipldemia and HTN.
Results: There were 81.1% patients with prior history of HTN and 100% hypertensive patients without prior history of dyslipldemia. All had health insurance.
Clinical Presentation: The average age was 65.58
± 12.20, average weight was 51.69 ± 8.19 (kg) (male:
54.83 ± 6.6; female: 50.25 ± 8.49), hypertensive patients with abnormal lipid level were 71.6% (male- 66.7%, female: 73.9%). The average length of stay was 8.29 ± 4.34 days, both of mode and median were 7, p < 0.01. Systolic HTN was 98.9%, diastolic HTN was 70.5%, both of systolic and diastolic HTN were 69.5%. p < 0.01. The 1st stage HTN was 33.7% while the 2nd stage HTN was 66.3%. p < 0.01.
Laboratory findings: There were 71.6% with lipid disorders. 41.1% patients with increased cholesterol level; 37.9% with Increased triglyceride level, p <
0.01; 42.1% with decreased HDL-c level, p > 0.05:
and increased LDL-c level was 25.3%, p < 0.01. The percentage of patients having 1 or 2 abnormal lipid levels were 50.5%, p < 0.01. The percentage of patients with abnormal ECG was 70.5%, p<0.01.
Correlations: There was weak positive correlation between the increased cholesterol level and increased weight, with r = 0 15; p > 0 05; strong positive congelation between the increased cholesterol level and increase6 LDL-c level, with r = 0.88; p<0.01;
negative correlation between decreased HDL-c level and incmased LDL-c level, with r = - 0.28; p<0.01.
Suggestions: To educate the ' public on dyslipldemia; to set up communication channels so the public can access physicians for consultation on lipid disorder and to establish special units for detection, tmatment and follow-up of hypertensive patients with dyslipldemia. Treatment of dyslipldemia is important to prevent future coronary artery disease.
Y HOC THirC HANH (940) - S f . T i ^ ^
