lugng cao hon so vdi loii A. capitatum. Nhung ket qui nghien cftu tren day khing Ham lugng polyphenol toin phin cua loii dinh o Vidt Nam cd tdn t^ mdt loii thuge chi A. hirsuta chidm 1,08% tinh theo ham lugng Adenosma khac so vol nhung loii hien dang acid galic li m§t trong nhQng kit qua mdi, dugc sft dung lim thuic. Vdd ngudn dugc theo hiiu biit cua tac gia chua co tii lieu nio lidu khi phong phi nen cin cd nhung nghien cdng bd vi dinh lugng ham Iugng polyphenol cftu sau hon vi tic dung smh hgc cfta loai toan phin trong cac loii thudc chi Adenosma. niy nhim gdp phin da dang hda nguydn lieu
4. Ket luan lim thudc sin cd tai Viet Nam.
T^i lifu tham ithao
r. Do Tdt Lgi (2004), Nhttng Ciy thu6c v4 Vi thu6c Vi$t Nam, NXB Y Hgc, pp. 625-629. 2. V6 VSn Chi (1997), Til diSn c a y thuoc Viet Nam, NXB Y hoc, pp. 863-865. 3. Pham Hoing Ho (2000X Ciy co Vi|t Nam, NXB Tri, QuySn II, tr. 903, 7635, 4. Tran VSn Sung (1997), Tsp Chl Hoa Hgc Nghiin cilu thJnh phin h6a hgc cCia loai Nhin trdn [Adenosma caeruleum R.B.R. (Serophulariaeeae)], 35 (2), 7-8.5. Nguyen Tiln Bin (1997), Cdm nang tra cuu vd nh^n biet cdc hq thuc vgt hgt kin a Viet Nam. NXB. Nflng nghiep, tr 59. 6. Le Dhih Bich, TrSn van On (2007), Thuc vgt hoc, NXB. Y hoc, tr. 399. 7. NguySn Vilt Thin (2000), Kiim nghlfm duvc Uiu bdng phucmg phdp hiin vl. NXB Y hgc, tap I. 8. Bg Y t l , Dw?c dlin Viet Mam III. tr. 434-435. 9. Ve Vfin Chi (2012), Tit Diln Ciy Thulc Vift Nam, tip 2, NXB Y Hgc, pp 351 -352.10. Viln Dugc liju (2006), Ciy thulc v i fi?ng vJt ilm thulc g V i | t Nam, NXB KH&KT, pp. 233-234. I I Vi|n Dugc li|u (2004), Cay Thuflc v4 Dgng VJt Lim Thuflc ft Vi|t Nam. nxb KH&KT, pp. 233-235. 12. De Abreu, M. B., malaftonte, N., Kiem, P. V., Braca, A. (2009), A new iridoid from Adenosma caeruleum R. Br. Fitoterapia, 80, 358-360.13. Phuong, N. M., Sung, T. v., Kamperdick, C , Adam, G., 1997, Methoxylated flavones from Adenosma capitatum, Phannazie, 52(8), 647-648. 14. The British Pharmacopoeia Commission (2009), British Pharmacopoeia 2009, 15. Elena T.
Tsankova, Liii V. Kulevaa, Le Thai Thanh (1994), Composition of the essential oil ot Adenosma bracteosum Bonati. Joumal of Essential Oil Research, 6(3), 305-306.
Tap chiDuwc lieu, tfp 18, s61/2013 (Trang 49 - 55)
NGHIEN CilV T A C D V N G CUA HON HgfP CAO CHIET TU" CAC DirglC L i f u B ^ C H QUA, HOANG K t , OAN SAM VA DUONG QUY LEN MQT SO CHI TIEU TIM M^CH, H U Y I I T AP VA DONG MAU
TREN DONG VAT T H ^ C NGHIEM
'c&n Vin Mio, ^Phgm Thj Nguyet Hing, 'Bd Thj Phuang,' Trdn ViftHung, 'Hgc Yi$n Qudn Y103, 'Vien Kiim nghiem thudc Trung uang, 'Vien Dugc lieu
(NhSn bai ngiy 22 thang 01 nim 2013)
Summary
Effect of Mixed Crude extracts {Ginkgo biloba Lin., Radix Astragali, Radix Salvlae mlltlorlilzae.
Radix Angelicae sinensis) on some Parameters Including Heart Contraction, Blood Pressure and Blood Coagulation in R a t
Researches to find out traditional medicines that have effective treatment on brain circulatory insufficiency ate being interested. Healthy rat were divided into two groups, including positive control group used p.o Ginkgo biloba extract with the dose of 24 mg/kg/day and treated group used p.o. mixed crude extracts with the dose of
Tap chi Duac lieu, tdp IS, sd 1/2013 49
152 mg/kg/day). Animals in bodi groups undergone surgery for vertebral arteries narrowed. After recovery (2 weeks), tail blood pressure, heart contraction and blood coagulation tests were performed before and after oral administration. The heart contraction was expressed by measuring the right intra-atrial and mitra-ventricular pressure. The results showed that mixed crude extracts (Gln/cgo biloba Lin., Radix Astragali. Radix Sahlae mtltiorhlzae. Radbc Angelicae sinensis) ameliorated brain circulatory insufficiency; increased heart contraction;
increased prothrombia time but there was no change in arterial blood pressure.
Keywords: Brain circulatory insufficiency. Glnlcgo biloba Lln., Radix Astragali, Radix Sahlae mllliarhizae, Radbc Angelicae sinensis, heart pressure.
1. Dat van de
Khi khdng dap ftng du nhu ciu dinh duong, khdng du oxy, nao khdng chi lim viec higu qui thSp ma cdn phat sinh benh, thiiu nang tuan hoan nao hay cdn ggi li thiiu mau nio.
Theo dinh nghia cua Td chfte Y te the gioi - thieu ning tuan hoin nio la trang thai nhat thdi, xiy ra dgt ng^t do cic thieu sdt ve chfte ning than kinh, thudng hdi phuc hoin toan sau 24 gid vi cd xu hudng l^p lai nhieu lan.
Benh thudng g^p d nhftng ngudi dung tudi.
Benh cd nguy co gay tft vong cao thft ba sau bdnh tim in?ch vi img thu. Ty 1$ thudng gip li 2 - 8/1.000 dan/nam va chiim 9-25% ting sd cic tai bien m^ch mau nao. Ty Id tai phat thanh tai bidn mach mau nio chiem 6-8%
trong nhftng thing dau vi 3-5% d mdi nam sau. Chi tinh rieng d My, chi phi y te cho bgnh niy chiem khoing 40 ty USD hing nam.
Hien nay da cd mgt sd thudc dugc sft dung cd tic dung dieu tri hieu qua benh 1^
niy. Tuy nhien, hieu qua vin cdn hjin chi va gia ca cdn cao, do dd, viec tim cac thudc ddng y cd tac dung dieu tri benh ly dang dugc cic nhi nghien cuu quan tim. Xuit phit tft thirc te tren, nhdm nghien cftu tiin hanh de tai nghien cftu tac dung hdn hgp cao chiit tft cac dugc lieu bsich qui, hoing ky, dan sam, duong quy nhim cii thien tuin hoan nao len mdt s6 chi sd tim mach, huyit ap vi mau tren dgng vat thirc nghiem.
2. Doi tirong vi phirong phip nghiin cihi 2.1. Boi tugng nghien cint.
Chu^t cdng tring khde m?inh 8-10 tuin
tudi (trgng lugng tft 200 - 300 gr) vdi si lugng la 40 con, do Ban ddng y?t Hgc v i ^ Quan y cung cip. Bgng v^t dugc chia thinh hai nhdm:
- Nhom ddi chiing ducmg: gdm 20 con dugc cho udng thudc cao bjch qui vdi liiu 24 mg/kg/ngay
- Nhdm thudc: 20 con dugc cho udng thudc thft vdi liiu 152 mg/kg/ngay
Thudc nghien cftu l i hSn hgp cao chiit tft cac dugc Iidu dugc cung cap tu de tii cap Sd khoa hgc vi Cdng ngh^ thinh phi Hi NOi
"Nghien cftu bao chi va dinh gii tic d\mg hojt huyet tren thuc nghi$m cfta hSn hgp cao chiit bio chi tft cic dugc li$u bach qui, hoang ky, dan sam va duomg quy" do TS.
Tran Vi$t Hiing lam chu nhiem di tii, nam 2011. Liiu udng dua trdn co sd liiu udng cfta bii thudc Duong quy bd huydt, gim hoing ky vi ducmg quy, cd bd sung them b?ch qui vi dan sam. Trdn co sd liiu uing niy, nhdm tie gii quy ra liiu uing tren dgng vJt.
DOng vat d ca hai nhdm dugc phau thu^t giy hgp ddng mjch ddt sdng hai bdn sau dd dugc chia diu thinh hai nhdm nhd (moi nhdm 10 con) cho 2 thi nghiem; Do huyit ip dudi chugt, do ap lire budng tim (tim nhi vi tim thit phii) vi liy mau lim xdt nghigm ddng miu toan bO.
Chudt dugc chim sdc v i nudi trong phdng du thoang mat, an uing diy dft, chu k^ sing tdi dugc duy tri 12/12 gid.
2.2. Phuangphdp nghidn cuu.
2.2.1. Chuin bi dOng vjlt:
Trudc khi tiin hinh nghien cftu 2 tuin
50 Tap chi Duac Ueu, tdp IS, sS 1/2013
dOng v^t nghien cuu d c^ hai nhom dugc phSu thu§t gSy hep d^ng mach ddt sdng hai ben.
> Phdu thudt gdy hep dong mcich dot sdng - Sau khi gdy me bfing thufic me Thiopentan [hang Rotexmedica, Diic] (nong dp 1%) voi liSu 4 ml/kg chuot dugc co dinh ISn he thdng c6 djnh Stereotacsic (Naghishigate, Nheit Ban) bang cac thanh co djnh vao hai tai, ham tren theo ky thugt co dinh chudn.
- Dong vdt dugc dat nam sap tren ban mo, cao sach 16ng va sat trung vung c6 sau.
- Boc 10 r5 16 d^ng mach dot s6ng a hai ben d6t s6ng c6 Cl, trong lo thSy dgng m?ich dot s6ng di qua.
- Dung dSu dot duong kinh 1mm cua mdy dot dien de dot Iam bit tdc dpng mach dot s6ng qua lo dpng mach d6t s6ng hai ben dot song c6Cl.
Dpng v^t dugc thodt mg sau 2 gib vd dugc sudi km, theo d5i trong 12 gia sau phdu thudt.
2.2.2. Cac chi ti^u nghiSn ciiu:
Sau phau thudt 2 tudn, chupt hdi phuc trd lgi binh thudng vd dugc cho udng thudc trong 3 tu4n. Trudc vd sau khi udng thudc chupt d cd hai nhdm dugc xdc djnh cdc thdng so sau:
> Ghi dp luc tdm nhivd tdm thdt phdi Sau khi dugc gay me bdng pentotan (n6ng dd 1%) vdi lieu 4 ml/kg, chupt dugc dat ndm ngtra tren ban md, vd phiu thudt boc lo ttnh m^h canh. Ludn canuyn (kim ludn kich thudc 22 gause) vdo tinh mach canh ben phai ngoai sau khodng 5mm. Qua canuyn ludn diu do dp lire SPR-1000 Millar Mikro-Tip
BSng 1. Anh huong cua hSn hop
(AD instrument, IJc) dudng kinh 0,33mm vdo tdm nhi phdi va ti^p tuc qua van ba la dk vdo tdm thdt phai. Ap l\rc tdm nhi va tam thit phai dugc ghi lai khi n6i ddu do dp luc vdi hf thdng ghi vd phan tich sd lipu Powerlab (AD instrument, Uc).
> Ghi huyet dp dong mgch
Hyet dp dpng mach cua chudt dugc do bdng phuong phdp do gian tilp qua ddng mach dudi:
Chugt dugc nam thodi mai trong budng ghi huyet ap.
Ddt bao dp luc va ddu do MLT44 (Powerlab, Uc) vdo gdc dudi chudt, ddu do huylt dp cdeh bao ap lire 2 cm va diing vi tri cua dgng mach dudi chupt.
Ndi ddu do dp l\rc vdi module ghi huylt ap tren ddng vdt (NIBP module) vd ndi module vdi hS thdng Powerlab.
Ghi vd phdn tich kit qud bdng he thdng thu thap, xur ly sd Heu Powerlab, phdn mim Labchart Pro (AD instrument, Uc).
> Xet nghiem ddnh gid cdc thong sS dong mdu todn bo.
Chudt dugc ldy mdu bdng kj? thudt ldy mau dudi chugt lam xet nghipm dinh lugng cdc thdng sd ddng mau todn bp. Thdng sd thu dugc gdm: thdi, thdi gian APTT (activated partial thromboplastin time), thdi gian PTT (Prothrombin time), ndng dp fibrinogen.
3. Ket qua
3.1. Anh hudng ciia hon hap cao chiit ISn tim mgch
3.1.1. Bien ddi siic co bdp cua tim:
cao chiet Ien &p lire tSm nhT ph^i
---_^__^^Th6i digm
Nhdm ddi chthig duong Nhdm thu6c
n 9 9 P
Ap lu^ tam nhi phai (mmHg) Trini'c u6ng thu6c
8,55 ± 4,01 9,22 ±1,93
>0,05
Sau u6ng thu6c 10,61 ± 3 , 8 7 11,94 ± 3 , 0 9
>0,05
P
>0,05
>0,05
Tap chi Daac lieu, tdp IS, so 1/2013 51
Kit qui Bang 1 cho thiy: sau udng thudc, ^ nghia thing kd (p>0,05), khdng cd sir khic ap luc tim nhi phai cua chudt d ca hai nhdm biet gifta nhdm chiing vi nhdm udng thuic d chftng vi nhdm thudc ting nhe so vdi trudc ci hai thdi diim tnrdc va sau uong thuic udng thudc nhung su khic bidt nay khdng cd (p>0,05).
Bang 2. Anh hudmg cua hon hop cao chiSt len 4p Iuc tam that phSi
^ — ^ ThMdiem j
^ ^ ^ • ^ n
Nhom ^ ^ Nhom dfli chiing duong 1 9 Nh6m thu6c i 9
P
Ap lire tSm thit phai (mmHg) Trird'c uong thuoc
41,79 ±14,02 42,52 ±7,18
>0,05
Sau u6ng thu6c 43,61 ± 19,64
50,88 ±1,.05
<0,05
P
>0,05
<0,05
Kit qua Bdng 2 cho thdy: dp luc tdm thdt cua nhdm thudc tdng len dang kl so vdi trudc phai cua chupt thudc nhdm chiing khdng cd sir udng thudc (p<0,05) va cao hem rd r^t so vdi khac bipt giiia trudc va sau khi udng thudc. nhdm chumg sau udng thudc (p<0,05) Ngugc lai, sau udng thudc, dp luc tdm thdt phai 3.1.2. Biln ddi huylt ap ddng mach:
BSng 3. Anh hu'dng ciia hSn hop cao chilt' len huyet &p tSm thu
^ ^ ^ ^ ^ T h 6 i tlilm Nhom ^ ^ ^ ^ ^ Nh6m d6i chung ducmg Nh6m thuflc
n 10 10 P
Huylt Ap tSm thu (mmHg) Tru^'c u6ng thu6c
99,13 ±11,23 97,24 ±13,37
>0,05
Sau u6ng thu6c 103,32 ±14,23 101,25± 11,86
>0,05
nfV.
, >0,05
>0,05
Bdng 3 cho thdy: Huyet dp tam thu cua chudt la khdng cd y nghia thdng ke (p>0,05) va khdng thupc ca hai nhdm tang nhe sau khi udng thudc cd su khdc biet giu^ hai nhdm d ca hai thdi dilm so vdi tnrdc udng thudc, tuy nhien sir khac biet trudc vd sau udng thudc (p>0,05).
Bang 4. Anh hudng ciia hSn hop cao chilt ISn huylt ^p tSm trirong
^ ^ ^ - . . . ^ Th6t digm Nh6m ^ ^ ^ - - . . . ^ Nhom doi chiing duong Nhom thuoc
n 10 10 P
Huyet &p tam trutmg (mmHg) Tru-6'c u6ng thu6c
48,39 ±6,31 51,42 ±5,25
>0,05
Sau u6ng thuSc 50,43 ±8,11 53,43 ±9,61
>0,05
P
>0,05
>0,05
Bang 4 cho thiy, huyit ap tam truang cia chudt thudc ci hai nhdm tSng nhe sau khi udng thudc so vdi trudc uing thudc, tuy nhien su khac biet la khdng cd y nghia thdng ke vi khdng cd sir khac bidt gifta hai
nhdm d ci hai thdi diem trudc va sau uing thudc.
3.2. Anh huang ctia hSn hgp cao chiit lln qud trtnh dong mdu.
3.2.1. Anh hudng len thdi gian APTT:
52 Tap chiDugc Ueu, tap IS, sg 1/200
Bdng 5. Anh hucmg ciia hSn h^rp cao chifit len thin gian APTT ,-^^Thifi dilm
m 6 m ^ ~ ~ ~ - ~ _ , _ ^ Nhdm d6i chthig duong Nh6m thudc.
• 9 9 P
Thiri gian APTT (gily) Tru6-c u6ng thu6c
14,34 ± 2,86 13,94 ± 1 , 5 6
>0,05
Sau u 6 n g t h u l c 16,51 ± 3 , 6 2 16,62 ± 4 , 4 4
>0,05
P
>0,05
>0,05
Bing 5 cho thiy, thdi gian APTT cua chuOt khdng cd sir khic biet giua hai nhdm d ca hai d ci hai nhdm khdng cd s\f biin dii dang ki thdi diim ttudc vi sau khi udng thuic (p>0,05).
giiia trudc va sau khi udng thuic (p>0,05) vi 3.2.2. Anh hudng ldn thdi gian PTT:
Bdng 6. Anh huong ciia h5n htfp cao chiSt len th&i gian PTT
^ ^ " - - - . . , T h M dilm NhSm ^""~-~^,^^^
Nh6m dli chiing duong Nh6m thulc
n 9 9 P
Thfri gian PTT (gifiy) T r u ^ ulng thulc
15,66 ± 1 , 1 9 15,78 ±,.29
>0,05
Sau ulng thulc 17,11 ± 3 , 8 7 26,97 ± 2,06
<0,05
P
>0,05
<0,05
Bang 6 cho thdy, thdi gian PTT ciia chupt tdng len dang kl sau khi udng thuoc so vci khdng cd s\l Ididc biet giQa hai nhdm d cd hai trudc khi udng thuoc (p<0,05).
thdi dilm trudc va sau khi udng thulc 3.2.3. Anh hudng l€n ndng d0 fibrinogen (p>0,05). Tuy nhien d cd hai nhdm, cd s\r huylt tuong:
B i n g 7. Anh hirdng ciia hSn hep cao chiet len ndng d^ fibrinogen
Nh6m ^~~~"-'---,,_^^
Nh6m d6i chCrng duong Nh6m thuoc
"
9 9 P
N i n g d9 fibrinogen (g/l) Tru^c u l n g thulc
4,67 ± 1 , 6 4 4,30 ±1,75
>0,05
Sau ulng thulc .7,46 ± 1 , 6 7 5,09 ±1,91
>0,05
P
>0,05
>0,05
Bdng 5 cho thdy, ndng dp fibrinogen trong huyet tuong cua chudt d ca hai nhdrn khdng cd sy biln ddi ddng kl giGa trudc vd sau khi ulng thudc (p>0,05) vd khdng cd su khac bi^t giita hai nhom 6 cd hai thdi diem truoc vd sau khi u6ng thudc (p>0,05).
4. Bin lu^n
Nghien ciiu tim kilm hon hgp cao chiet ddng y Id hudng uu tien trong duong ldi kit hgp hai nen y hgc hien d^i va cd truySn d Vi§t Nam. D3 cd nhilu cdng trinh nghien ciiu
vl tdc dung cOa m^t so hSn hgp cao chilt dnh hudng len tudn hodn ndo vd mgt so sdn pham thuoc ddng y dd dugc mang vdo sii dyng trong lam sang cd kit qua dieu tri tit.
Nghien ciiu sti dung nhdm ddi chiing la cao bach qud. Id mdt dugc li$u dd dugc nghien ciiu rat rgng rai cd trong nudc vd qudc tl Id cd tac d\mg tdng tudn hoan ndo, cdi thipn tri nhd, cdc tri|u chiing ciia tinh tr^ng thilu nang tudn hoan nao [3,7].
Trong cdc nghien ciiu trudc day ve tdc
Tap chi Duac Ueu, tdp 18, s^ 1/2013 53
dung cua cao bach qua len tim m ^ h deu cho thdy cao bach qua it anh hudmg din siic co bdp cua tim khdng lam gidm huylt dp md chil ylu la tdng luu lugng mgch mdu nao, gian mach trung tdm, cai thien tudn hoan nao [2, 5]. Nghien ciiu nay ciing thu dugc kit qud tuong tu: chudt thudc nhdm chiing, sau udng thudc 3 tudn, khdng cd su bien ddi nhilu ve chi sd dp Iuc trong budng nhT trai, budng thdt phai so voi truoc udng thudc. Tuy nhien, chudt thuge nhom thudc thu lai cd chi sd dp lye thdt phdi tdng hem so vdi trudc ulng thudc va cd s\r khdc biet so vdi nhdm chiing d thdi dilm sau udng thudc, day Id sy: khac bi?t cua hdn hgp cao chilt so vdi cao bgch qua. Trong cdc nghien ciiu ve dan sam, duong qui cac tdc gia da cho thay: dan sam, duong qui cd tac dung bao vp co tim, tdng tuan hoan vi mach [3, 6], do do tdc dung len tim cila hon hgp cao chiet co the do tac dung cua dan sam va duong qui trong thdnh phdn cua thudc. Ddng thai ket qua trong nghien ciiu nay cho thdy khdng cd sir thay ddi dang kl ve huylt dp tdm thu vd tdm truong d cd hai nhdm trudc vd sau udng thudc. Nhu vdy hon hgp cao chiet lam tang siic co bdp ciia tim nhung lai khdng tang huyet ap, ket qua nay cd the do tdc dung gian mgch cua hon hgp cao chiet nen da bu tru vol tac dung tdng CO bop cua tim nen khdng Iam tang huylt dp, dilu ndy cung phu hgp vdi tdc dung ciia thdnh phdn bach qud va dan sam, duong qui cua hon hgp cao chilt [3, 5, 6]. Vdi cac dnh hudng len tim mach cua hdn hgp cao chilt cho ta thay hon hgp cao chiSt cd tac dung ldm tdng siic co bdp ciia tim, gay gidn mgch vd khdng dnh hudng len huylt dp.
Nghien ciiu vl dnh hudng cua bgch qua len tac dung chdng ddng mdu, hdu hit cac nghien ciiu diu cho thdy bach qud cd tdc dung hoat huylt nhung khdng lam anh hudng
din qud trinh ddng mau [3. 4]. Nghidn ctru nay cung cho thiy cao bgch qud khdng dnh hudng 16n thdi gian APTT, PTT va ndng dO fibrinogen trong huylt tuong. Anh hudng ciia hdn hop cao chilt len cac chi sd ddng mau cho thdy, sau khi udng thudc chugt d nhdm thudc cd chi sd thai gian APTT va ndng d$
fibrinogen huylt tuong khdng thay d6i nhung thdi gian PTT thi tang ldn ddng kl, dilu ndy cho thdy hdn hgp cao chilt gay keo ddi thdi gian ddng mau thong qua tdc ddng Ien cdc ylu td ddng mdu qua con dudng tgo prothrdmbinase ngogi sinh ma khdng dnh hudng qua dudng ndi sinh ciing nhir fibrinogen trong huylt tucmg. Tdc dyng ndy ciia sdn phdm nghien cihi Id sir khdc bi^t so vdi tac dyng ciia bgch qud co th6 la do tiidnh phdn, ducmg qui, dan sdm va hoang ky cua h5n hgp cao chilt gdy nen [3, 7, 8, 9]. Nhu vay hon hgp cao chiet nay ngoai tdc dyng hogt huylt bdi thdnh phdn bach qua cdn cd tdc dyng keo dai thdi gian ddng mdu qua con dudng ngoai sinh, dieu ndy giiip tac dyng hoat huyet mgnh han, giiip cho dong mdu qua ndo nhidu ban, di dang hon d6 chlng Igi tinh trgng thieu nang tu4n hoan ndo. Ket qua nghien ciiu ndy CO sy tuong ding vdi kit qud ciia mpt sl thudc cd tac dung cai thien tudn hoan ndo md eac tac gia khac da nghien ciiu [4,6,8].
Theo quan niem y hpc cd truyen thi bgch qud, hoang ky cd tac dyng bd khi, duong quy, dugc su dyng dl dilu tri cdc trudng hgp ty khi hu nhugc, khi huylt ludng hu, ty phi khi hu, huylt khi td dgi chdn tay, trung phong... Trong khi dan sdm, duang quy cd tdc dung bo huyet, hogt huylt, d£mg dl dieu tri cac chiing tdm can huylt hu (hii hOp ddnh tring nguc, cam gide bdn chin, hoa mdt chdng mdt...). Vi$c ket hgp cdc vi thulc c6 tac dung bd khi vdi cac vj fliudc co tdc dyng bl huylt, hogt huylt vdi ^ nghia Id hb khi de
54 Tap chi Duac liia, tdp 18, sd 1/2013
sinh huyet vd cd tac dyng dieu tri cac chiing tdc dyng: Khdng lam biln ddi huylt dp ddng hylt hu, khi huylt hu tuorng duang vdi cdc mgch, tang siic co bdp cua tim, ldm tdng thdi bilu hipn b§nh I;^ theo y hgc hidn dai nhu: gian ddng mdu qua con dudng npi sinh, tdc thilu ndng tuin hodn ndo, tdng huylt dp, suy dyng hoat huylt dudng nao mgnh hon so vdi nhugc ca thi, suy tun... [3] tac dung cua cao bgch qud don thuin.
5. Ket lu^n Ldi cdm an: Nhom nghien cmi xin cdm an Sa HSn hpp cao chilt bdo chl tii cac dugc lieu Khoa hoc vd Cong ngh? thdnh phd Hd Ngi da hd b?ch qua, hodng ky, dan sdm, duang quy cd tra/dnh phi di thuc Men di tdi.
Tai li^u tham khao
1. BO m6n Dirgrc li?u, Trudng d^i hpc Dir(?c Ha NOi (1980), Ba/g/angrfi/{re//eu.-/a/) / . NXB Y hpc H i NOi. 2.
Brinkley TE, Lovato JF, Arnold AM, Furberg CD, Ginkgo Evaluation of Memory (GEM) Study Investigators (2010). Effect of Ginkgo biloba on blood pressure and incidence of hypertension in elderly men and women. Am J Hypertens. Volum 23, issue 5, p 528-33. Epub 2010 Feb 18. 3. D 5 T^t Lpi (1995), Cdy thudc va vj thudc Vi?t Nam. NXB Khoa hpc ky thu^t. 4. Keun Ho Ryu. Hye Young Han. So Young Lee (2009), Ginligo biloba extract enhances antiplatelet and antithrombotic effects ofcilostazol without prolongation of bleeding time. Thrombosis Research, Volume 124, Pages 328-334. 5. Hirovasu Satoh. SeJichiro Nishida (2004), Electropharmacological actions of Ginkgo biloba extract on vascular smooth and heart muscles. Clinica Chimica Acta.,Volume 342, Issues 1-2, Pages 13-22. 6. Xun-Lan Lei and George C Y . Chiou (1986), Studies on Cardiovascular Actions of Salvia miltiorrhiza. Am. J. Chin. Med, Volum 14, issue 26 p 15-22. 7. Yi-Chian Wul and Ching-Liang Hsieh (2011), Pharmacological effects of Radix Angelica Sinensis (Danggui) on cerebral infarction. Chinese Medicine, p 6:32. 8. Yang T, Jia M, Mei Q, Shang P. (2002), Effects of Angelica polysaccharide on blood coagulation and platelet aggregation. Zhong Yao Cai, Volum 25 issue 5, p 344-345. 9. Meen Saleh Ahmed Bin Bisher (2009), Tifie Physiological Changes in Blood Coagulation Parameters Induced by the Therapeutic Doses of the Chinese Danshen Plant (Salvia miltiorrhiza) in Male Guinea Pigs (Cavia porcellus). Joumal of Biological Sciences, Volum9, issue \,pTi-ll.
Tgp chiDi^gtc lieu, tap 18, so 1/2013 (Trang 55 - 61)
XAY m^G PHXrONG P H A P DJNH LlTONG Z-LIGUSTILID TRONG O r g f C L i f u D i r O N G Q U I N H A T B A N D I THlTC BANG KY T H U ^ T
SAC KY LONG H I E U NANG CAO
Nguyin Thi Hd Ly, Nguyin Thi Phuang, Nguyin Thi Nggc Lan, Nguyin Thi Bich Thu, Nguyin Minh Khdi, Phwang Thien Thirang
Vien Duac li^u
( N h a n bai ngay 21 thang 11 nam 2012) Summary
Development of a Method for Quantitative Analysis of Z-Ligustilid fn the Roots of Angelica acutiloba by High Performance Liquid Chromatography The compound Z-Iigustilid is one of major compounds of Danggui {Angelica spp) in the oriental medicine. In this report, we established a method using HPLC for quantitative analysis of this constituent from the root of
Tap chiDugc lieu, tap 18, so 1/2013 55
