• Khoa hpc Ky thuit vi Cdng nghi
Phan lap va tuvin chon chiing vi khu^n CO kha nang khii mau nu-oc thai det nhuon
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i:hu\cn plian hit" iil'J, n^av nh3iiplia
^^"^""^ .- . ^ - - . - . o n h i l n , moi t r u 6 i . g u g h i e m t r p n g . Nhi^u Nude Ihai d?t nhuym khong qua \ir h . \ a Ir^rc tiep vao nguon niro-c SL g.iv o ntiiem ^^^ ^,_^ ^^^ ^^^.^^ ^ ^ ^ ^ phirmiH p h i p V ai Iv. hoa h^>c n h « Ipe. kit tua. keo tg 6k dirge tien hanh. luv " ' " ^ " -'• j ^ ^ , ; ^gt „ h u 6 m d u g c hr^nif;. lao raclKii thai khi. x u l v . anh h t n m s ldi hv sinh thai. .Sir d u n g viMUh vat a e ^ ^ ^ ^ ^
xem h. phutmn phap thav thi^ v. gia thanh khimg cao. than ihien vdi mm irirotig. Nh.eu c h u n . chi vi k L a n . vi n i n . . va k h u i n v.. ,a.. eo kha n . n g phan huv ,hu6c nh.i(MU. Nghien eiru nay ^ ' ^ 9 ; . ' ' ^ ^ ^ ^ ' ' ' ^ " j ; ; 7 , ^ i dich khao . . . kha nang k h u mau thuAe nhunm Red I N : H . eua mC-. sA chung vi k h u a n d u p e p h a n ^ ^ P * Y " " ' * , ^ d(.. nhtii.m, Ba ehung vi khuan v:, ^9 ^ . VI4 eo hi^u qtia k h u mau da dugc tuven ' = ' ' 9 " : . " f " / ! ? \ ^ , ^ ™ . ' " ' ehung V. (S.),.,"..i - M 4 ^iC.S-'.^ - M (.M.6%) .rongdic^u ki^n nuoi tinh; trong di^u kifm nuot lae, h . u q u a p h a n h u y th.n'.c nhupm eua chung V> (fi.V^-..l M 4 (34,9".,,> V2 , 2 6 . 9 % ) . C h u n g Vi d u p e ehpn de khao s^t a n h h u O n g cua mi}l si, dl^-u kivn moi i n n i n g nuoi eav. Mi^u qua k h u mau eua chung \ 9 m a n h nhk khi trong mot truffng n u o i cay CO nguon earl.on la tinh b(.l. nguon nito-la ( N H . I . s o ^ . \ H , ( I hav cao nam men, mat dp vi k h u a n 5-15/«, p H mot truiyng i n m g khoang (.-^, nhipl dg 35 < . C hung .\9 et. liim n.ing tmg d u n g \u- Iy nir6e thai det n h u p m .
Ill khmi. khir mdu. nutre thai, phan huy sinh hpe. Ihuoc n h u p m . Chi sd Khan loai: 2.^
B«t rtn dA
Trong nganh cong ngluep del nhugm. hang nam khoang 200.01)0 tim ihuoe nhupm cac loai dupc thai ra ducn dang niroc thai trong qua trinh nhugm va hoan lhi?n san pham (I ]. Phan Ittn cac loai thuoc nhugm ton lai rat lau trong lu nhicn do linh on djnh cio cua chiing doi voi anh sang, nhiel dp. Xa tare liep mrac ihai tir qua trinh del nhupm. in vao nguon nuoc se gav 6 nhiem nghiem trong Thutjc nhuom irong nuoe lam t.^ng dg due. khong chi gay giam dp lioa tan o\v trong nuac ma con lam g u m kha nang \u>en qua nuoc cua anh sang m^l ircn, da do anh huong den quang hop va loan bg he sinh thai duai nuac [2] \ a nuoc thai del nhugm vao JO. ha. song --e lam ihav doi pH va tang nhu cau o w sinh ho.i, nhu c.iu oxv hoa hoc va long carbon huu ca [?].
Moi Ml tluiiv nhupm nhu ayo va cac ^an pham phan huv chung CO null diV. CO kha ri.ing gav ung thu. anh huong tai sue khoe con ngiiai Miicu plurong pliap \,\i K, hoa hgc nhu ivv. kcl lua. keo ly d.'i duge licii hanli luv nhien gia thanh CIO. lieu thu nhicu nang Iuang, lao ra that thai kho xu K ciing nhu anh huitng tai he vmh ihai [4 S] I'liucmi; phap sinh hi,H.- duoc \ e m la giai phap ihav ihe hicu qua vi iiia thanh khong eao, than ihien vai moi trtriTng \i<\ Khu mau nuoi, thai del nhugm co the dien ra ihco hai each hap r-h-
iren smh kh6i vi smh vat hay phan huy sinh hgc bai Ic bao VI sinh vat [I], Hien nay, bien phap phan huy sinh hoc d u g c img dung de loai bo nhieu chat gay 6 nhiem. Nhieu loai vi sinh val, gom vi nam, vi khuan, vi lao, nam men co kha nang loai bo nhieu dang thuoc nhupm [6]. Cac loai vi sinh V at sinh long hgp nhieu enzyme can thiet cho qua trinh lam mat mau va khoang hoa thuoc nhugm trong cac dieu kien moi inrcmg thich hgp [4]. Tren c a sa do, nghien cuu nay dugc lien hanh voi muc dich phan lap, tuyen chgn chung vi khuan tir nuac thai lang nghe del nhupm va khao sal anh huong cua mgl so dieu kien moi Irucrng toi kha nang lam mat mau thuoc nhuom Irong dieu kien in vilro.
Vdt lieu va phUfTng ptiap ngtiien ciia i'di lieu
Trong nghien ciru nay thuoc nhuom Red F N 2 B L i Reactive dye) vai /.-526 nm da duoc su dung, Niroc thai del nhuom dune lay tu lang nghe del nhuom phuong Van Phuc. quan Ha Dung. 1 P Ha Ngi.
Sang loc Idn ddu <. a nhudm
• chung vi khudn khir mdu ihudc Phan lap \ i khuan lu nuoc tliai del n h u o "
KHOAHOC \ l . \
U>NCNG«\iet V:!"
Isolation and characterisation of decolorising bacterial strains
isolated from textile dyeing wastewater
Huy Thuan Nguven'. Van Giang Nguyen'".
Thi Van Anh Le*
'Instilule of Research and Development. Duy Tan Universit}
•Faculty of Biolechnology. yielnam National Universm- of Agriculture Received 22 October 2019; accepled 3 Januarj 2020
/tbstract:
Directed discharge into water sources of untreated textile dyeing wastewater will cause seriously environmental contamination. Various kinds of physico-chemical methods have been in use for the treatment of the wastewater. However, these methods are not environment friendly and cost-effective, and hence become commercially unattractive. Many microorganisms strains belonging to the different taxonomic groups of bacteria, fungi, actinomycetcs, and algae have been reported for their ability to decolourise dyes. This study was conducted to investigate the decolorisation of Red FN2BL b\ using bacterial strains isolated from textile dyeing wastewater. Three different bacterial strains A2, A9 and A14 exhibited high decolorization effects were selected. Decolorisation effect of strain \9 was 80.6%>A14 (67.5%)>A2 (34.6%) in stable cultural condition and was A9 (63.3%)>A14 (34.9%)>A2 (26.9%) in shaking cultural condition. The strain A9 was selected for evaluating the effects of some cultural conditions.
Strain .A9 showed maximum decolorisation ability at pH between 6 and 7, temperature of 35''C, and bacterial density of 5-15%. Starch and (NH^),SO^, NH^Cl or yeast extract were found to be the optimum condition for decolorisation. In brief, the strain A9 reveals a great potential for application in the textile dyeing wastewater treatment.
bang each pha loang mau nuoc thai trong nuoc cat v a ca\
chang tren dTa thach dinh duong. Cae dTa th^ch nay dugc it 6 37-2T trong 24h Nhung khuan lac vai hinh thai khac nhau dugc lach rieng va nuoi cay lam thuan [7], Cac mau
\i khuan sau khi da duoc lam thuan dugc nuoi eav trong moi truong dinh duong long NB (gom (g/l): Peptone 10.
Beef exU^ct 10. NaCl 5. pH-7.3±0.l) trong 24h Iruoc khi ca\ ehuven sang moi trucmg muoi khoang loi thieu (gom (gl): (NHJ.SO^ 2. ICHPO^ 1, KH,PO, 1. MgS0,.7H,0 0.1, NaCl 5."glucose 3^ nuoc cdt 1000 ml. pH-7). Sau 24h nuoi. 10% (vv) dich nuoi cac chting vi khuiin dugc chuyen sang ong nghiem co chira 200 mg/l dung djch thuoc nhupm.
Quan sat su khir mau ciia cac chung v i khuan sau 4Sli ii cac ong thi nghiem a 30±2°C, Chiing nao bieu hipn liem nSng khir mau manh nhal dugc chgn de tien hanh cac thi nghipm tiep iheo [8].
Sdng lpc ldn cudi vd ddnh gid hi^ii qud khir mdu thuoc nhuom ciia cdc chung vi khudn phdn Igp
Thi nghiem nay dugc lien hanh bang each sir dgng thuoc nhugm Red FN2BL trong moi truang MS king.
Moi chiing vi khuan dugc chpn dugc nuoi 24h irong moi truang NB, sau do chuyen 5% (v/v) ciia moj chung sang binh Erlenmeyer(V=250 ml) chira 50 ml moi truirng MS va 100 mg 1 thuoc nhupm Red FN2BL Cac binh nay sc dugc nuoi cay a 30±2°C, trong thai gian 24-72h, dieu kign tmh va lac (150 vong/phiit). Dp hap thu anh sang cua cac mau ihi nghiem dugc do tai ?t=526 nm a thai diem 24 va 72h [8], Dua tren ket qua giam dg hap thu, % khir mau thuoc nhugm dugc linh loan, chiing vi khuan co liem nang khir mau thuoc nhugm cao se dugc chpn cho cac thi nghipm lii}p theo.
Hieu qua khu mau thuoc nhupm dtrpc bieu hipn bang %, 5 ml mau thi nghiem da khir mau dugc ly lam 10.000 vong/
phiit trong 15 phul va do do hap thu anh sang lai X=526 nm, Ong doi chimg chi chira thuoc nhugm va moi truang nuoi chung vi khuan. Thi nghiem dugc lap lai 3 lan. Hieu qua loai bo mau cua cac chimg vi khuan ihi nghiem dugc xac dinh theo cong thirc sau [8]:
Keywords: biodegradation, decolorisation, wastewater.
Classification number: 2.7
dyes,
Hieu qua khir mau (%) = [(1 - F)x 100]/1
fl)Trong do: I la do hap thu ciia ong doi chirng; F la dp hap thu cua ong thi nghiem.
Khdo sdt dnh hir&ng ciia mpt so chi tieu den khd ndng khir mdu cua cdc chiing vi khudn thi nghipn [5]
Anh hirijng ciia nong do ihuoc nhupm Red FN2BL: cac chiing vi khuan ihi nghiem dupc nuoi 24h trong moi trucmg NB. sau do 5% (v \) dich nuoi vi khuan duoc chuyen sang binh Erlenmeyer (V=250 ml) chira 50 ml moi Iruong MS dugc bo sung 100, 200 va 300 mg/l thuoc nhugm, pH=7.
Cac binh nay se dugc nuoi cay 6 30+2'X', irong thoi gian
72h. dieu kien tmh. Hieu qua khii mau dugc linh iheo cong
thuc (i).
t vi Cdng nghi
f
Anh hmmg cuu n Ic licp giong cac chung \ i khuan tin nghK'm dugc nuoi 24h trong moi truiTie NB. *au do lan lugt bo sung VI khuan a cac nong do khac nhauiv v ): 1,5. 10 va 15'V. vao binh Frienmevcr i \ -25ii ml) cei chua 50 ml moi trutmg MS v a 100 mg I ihu.x: nhuom. ehuan do nioi tnn-Tic ve pH " Cac binh nav s2 dugc nuoi eav a .'ib2 C. irong i h ^ gian 72h. dieu kicn tinh Hi^u qua khu mau dugc tmh nhu cong thuc 111
Anh hmmg cuu pli nhi('i dd ac ehung \ i khuan ihi nghifm dugc nuoi 24h irong moi truang NB. sau do 5*^0 iv v i d)chnu6i vi khuan duoe ehuvcnvjo binh Frlenmc>er(\ -2>M ml) chira 50 ml moi truang MS duoe Ki sung ilKhiig 1 ihuoe nhugm pH cua cae hinh ihi nghiem duoe Jieu chinh dat s, t\
7 V J S Cac binh nav duoe nuoi e.'iv a .'Or2"C, trong ihai gian 72h. dieu kii*n iTnh Tuong ty nhu va\. chinh pH eua hinh ihi nghidm de d^i gia in pH^ ~, nhung cac binh dugc u a nhiei do 15, 25. 30 v.i 411 t , irong ihtti gian "2h. dieu kicn linh Hieu qua khii mau dugc tinh iheo cong ihirc (I).
Uih hmmg cua ngudn carbon \d niiir cac chting \ i khu.in thi nghidm duoe nuin 24h irong nioi uvcmg NB.
sau do 5"n (V V) djch nuoi vi khuan dugc chuyen vao binh Erlenmeyer i \ - 2 5 0 ml) chua 5o ml moi truong MS dugc bfl sung 100 nig I thuoc nhugm, nguon ducmg glucosic trong moi inrone MS duoe Ihav ihe bang Saccarose, tmh bgt, Lacio-.e. \vio-.e, Mallo-e va Fructose. Tien hanh thi nghiem tuang Ur. niurne ihav nguon (Nil i.SO trong moi Iruong MS b.ing NMC'l. NaNO . KNO \ a peptone, cao thit, cao nam men. Cae binh n,iv liuoe nuoi eav a 30±2''C, irong ihoi gian ~2h, a dieu kien tmh. Hi^u qua khir mau duge tinh theo cong thirc (1),
K«t Qua va tuat iBiB
AW qud phdn lap chiing vi khudn tir niro'c thdi del nhupm
Mpl so khuan lac eo hinh thai, mau sac khac nhau da
\uai hicn iren dia moi tiirang thach dinh duong sau khi de dia o 3"'C ,sau 24li (hinh I). Cac chung vi khuan phan lap lir niju nuac thai thu tai lang nghe del nhupm phuong Van Phiic. quan Ha Dong. TP Ha Ngi duoc ldn luot dat ten la AI d^nA14.
Nhieii nha oghien euu da phan '-'ly''-'' \\^^\:^x nhie-m M khuan khu mau nuac tha. nhuoin [U^. ^ . ^ . ^ ^ j,^j^|^
thu.v nhugm azo [S]. Kel qua nay cho ^^^ _^^ ^ ^ . ^^.^
nehi tu nhicn ciia cac chung vi khuan -^_^-^^ s6t irong i-'v-rig hi nhiem ihuoc nhugm va Ktui n-in^-. • - dieu kiv'n CO thu6c nhugm dpc hai.
Sin, l,c lin Ma eae ehttn, vi ^httin ^"^ " ' ° " ' " " - nhupm
a n u . , C , . „ , ..-.an D , ^ - ; ; - : : ^ r . m l d,n., Jmic lam Ihuan Juoc nuoi ca> -41i tron^ . ,^.°,„,,;;, d u ™ . lone NI! iruac klu c.« chujcn sang nu>, tmcmg m u o , k h o a n . u-.; ,h,cu. sau 24h nuo,. 10% 0 > > d,ch num ca C h u n . ; , khuan dugc clunen sang ong ngh.em co chua 200 m . f d u n g d,ch .hu6c nhupm. Ket qua khu mau cua cac ch'ung >, khudn sau 4Sh u cac ong th. ngh.em a 30±2 C dugc the hi?n a hinh 2.
'7»-»^--.^ --r
EX A l v : AJ A4 A5 VG A7 A8 A9 A 1 0 A 1 1 A I 2 A 1 3 A l
Hinh V Khuin lac chung A2, A4 va A5 phan lap dugc \M nu thii d^t nhu^m
Hmh 2, Ket qua sang Ipc cac chiing vi khuan co kha nang khu- mau thuoc nhupm,
Kel qua quan sat cho Ihay, chiing A2, A9 va A 1 4 bieu hien hieu qua khu mau thuoc nhugm manh nhat, d u g c chpn de lien hanh danh gia hieu qua khir mau trong thi nghiem liep Iheo. Anamika va cs (2013) [8] cung da sang lpc d u g c nhieu chung vi khuan co kha nang khtr mau mgt so loai thu6c nhugm nhu Balck W N N , Blue FRN, Blue RC, T U R Q blue, nhung chi sang lpc dugc mgt chiing vi khuan khir mau thuoc nhupm Red FN2BL la thuoc nhugm d u g c sir dung u-ong thi nghiem nay. Ogugbue va Sawidis (2011) [1] da niyen chpn dugc 5 chiing vi khuan co kha nang khti mau thuoc nhugm Basic violet 3, Trong thi nghiem ciia minh, Ken Meerbergen va cs (201 8) [2] da tuyin chon d u g c cac chung VI khuan thugc chi Acinelobacler va Klebsiella biSu hien tiem nang khu mau thuoc nhuom azo. T u cac mau n u a c thai thu thap tai vimg bi nhiem thuoc nhupm. Maulin va cs 12013) [4] cung da phan lap d u a c 50 chun;; \ i k h u i n co ihe ;.v.i bo thuoc nhuom Nliu vdy co the ket luan rang, cac Chung vi khuan mai duoc phan lap tir nuoe ihai del nhuom CO the song \ a smh truong trong moi truong co cac loai ihuoc nhugm khac nhau.
KHOAHOC \
Sdng lpc lan cudi vd ddnh gid hieu qud khir mdu thude nhupm cua cdc chung vi khudn mai phdn lap
Chiing vi khuan A2,A9 vaA14 dugc nuoi 24h irong moi trucmg NB, sau do chuyen 5% (v/v) ciia m6i chiing sang binh Erlenmeyer (V=250 ml) chua 50 ml moi truang MS va 100 mg/l thuoc nhupm Red FN2BL. Cac binh nay se dugc li tai 30+2°C, trong thoi gian 24-72h, a dieu kien tinh v a ldc (150 vong/phiit). Hieu qua khir mau (hinh 3) ciia chiing .^9 (80.6%)>A14 (67,5%)>A2 (34,6%) trong diiu kien nuoi tmh, con trong dieu kien nuoi ldc, hieu qua phan huy thu6c nhupm ciia chiing A9 (63,3%)>A14 (34,9%)>A2 (26,9%) Ket qua sang Igc cho thay, hieu qua loai bo thuoc nhugm ciia cac chiing vi khuan thf nghiem trong dieu kien linh cao hon trong diSu kien lac. Thi nghiem ciia Anamika va cs (2013) [8] cung cho thdy, khd nang khir mau thuoc nhupm ciia cac chiing vi khudn trong dieu kien tmh cao hon irong diSu kien I5c. Theo cong b6 ciia Chen va cs (2003) [10], vi khudn Aeromonas hydrophila khir mdu thuoc nhugm (80%) trong diSu ki^n tmh (ky khi hay higu khi). Wang va cs (2009) [11]
Cling ghi nhdn hieu qua khii mau thuoc nhugm ciia chiing vi khuan dat 96% trong dieu kien ky khi, va chi dat 13%
trong dieu kien hieu khf, lac 150 vong/phut. Tuy nhien, mot so ket qua nghien cicu ciia Ogugbue va Sawidis (2011) [ 1 ], Saad El-Din Hassan va cs (2015) [5] cho thdy, hieu qua khu mau thuoc nhugm ciia cac chiing vi kliuan thf nghiem cao nhat trong dieu kien hieu klii (nuoi lac), Nhu vay, hieu qua khij mau thuoc nhupm cua cac chiing vi khuan khong chi ph^i thupc vao dieu kien hieu klif hay ky khf, co the con phy thupc vao cac yeu to kliac nhu khd nang sinh truang, long hgp enzyme phan huy Ihuoc nhupm ciia chung vi khuan.
Hinh 3, Kha nang khip mau thuoc nhugm ciia chiing A2,A9vaA14 sau 72h trong dieu kien ITnh (T) va lac (L),
Kit qua thi nghiem nay cho thay 3 chimg vi khuan A2, A9 vd A14 la cdc chiing vi khuan ky klii khong bat bupc, oxy can thiel cho sinh trudng ciia vi khudn, nhimg lic che hieu qua khii mau ciia chiing. Dua tren hieu qua khir mau, chiing A9 dugc chpn de lifin hanh cac thi nghiem tiep theo va dupc nuoi trong dieu kien tmh.
Khdo sdt dnh hirang cua mot so chi tieu den khd ndng khir mdu cua chting vi khudn A9
Anh lur&iig ciia nong do ihudc nhuom Red fSlBL sau
72h nuoi cay voi cac nong do thuoc nhupm khac nhau, hieu qua khu mau ciia chiing A9 dat 76.7-81.3" a phu ihupc vao nong dp thuoc nhupm (bdng I), Khi tang nong dg thuoc nhupm, hieu qud khir mau ciia chting A9 giam. Hi?u qua khu mau ihuoc nhupm ciia cac chiing vi khuan giam khi lang nong dp thuoc nhugm da dugc nhieu nha nghien cim cong bo, Saad El-Din Hassan vd cs (2015) [5] khi khao sat anh huong ciia nong dg thuoc nhupm lot hipu qua khii mau ciia cac chiing vi khuan Klebsiela da ghi nhan kel qua khi none dp thuoc nhupm tang, hieu qua khii mau cua cac chiing V i khuan giam ro ret. Nong dg thuoc nhupm Reactive Black 5 nhieu hon 200 mg/l da giam hieu qua khir mau ctia chiing VI khudn Enlerohacler sp. [11]. Hai chiing vi khudn Enterococcus faecalis va Klebsiella variicola da giam kha nang khu mau Ihuoc nhupm khi tang nong dp thuoc nhupm tir 50 den 100 mg/l [6]. Nong dp cao cdc loai thuoc nhupm irc che kha nang loai bo mau lir ca chal ciia vi khudn vd anh huong toi sinh trucmg, trao doi chdt va long hgp enzyme ciia te bao vi khuan [1.5].
Bang 1. Anh huwig cua nong dp thuoc nhupm to-i khd nSng khi>
mau cua chung vi khuan A9.
None dQ Ihuoc Gifi trj OD o, , . . • >
nhugm (mg/I) Doichune ThinelxiSm a.209
0.694 1.439
0,039 0,151 0.335
Anh Inrang cua ly le liep giong: hieu qud khir mdu thuoc nhupm ciia vi sinh vdt phu thupc vdo nhieu qua trinh nhu phan huy sinh hpc, hdp phu sinh hgc, kel liia [5]. Vi the, sinh khoi te bao co vai tro quan Irpng trong qua trinh khir mau nuac thai. Ket qua thi nghiem ciia chiing toi (bdng 2) cho thdy, khi mat dp vi khudn tang tir 1 den 15%, hi?u qua khir mau tang tucmg img tir 40,0 den 88,0%,
Bang 2. Anh hudng cua ty le tiep giong toi kha nang khi> mau ciia chung vi khuan A9.
Ty 1? tiep None 6^ ^'^ '"T OD , , , u • * giong (%) ttiuoc nhupm £,^- ^hihtg Thf nghidm
0.130 0,039 0,035 0,025
40,0 81,3 B3.3 88.0
Hieu qua khir mau cao nhat (88,0%) dat dugc khi lugng giong vi khuan ban dau la 15%, Mohan vd cs (2013) [12]
ghi nhdn chiing Bacillus sp, va Planococcus sp. dat hipu qua khir mau cao nhal khi nong dp chung giong ban dau la 10%
va thap hon a cdc nong dp 1, 2 vd 5%. Ket qua tuong tg cung da dugc cong bo boi Kumar vd cs (2009) [13].
.4nh Inr&ng cua pH, nhiet do- pH ciia moi trucmg nuoi
I Khoa hpc Ky thuit vi Cdng nghi
c^y dong vai tro quan trong Joi vm hoat dgng sinh 1> CI:J cue ti- bao VI khuan va >u trao doi cua cac thanh phin dmh dm>ng kha^ nhau qua mang. Hi?u qua khu mau cua ehung VI khuan A'J Jai cao nhat lai pH=t>-". khi lane hay g u m eia trj pH so vol <>-". hiv'uquakhumau deu giam ibang .'i "^.ud Fl-I)in Hassan va cs | 2 0 1 ? | [5] da ket luan nine pH loi UL de d^i hii-u qua >.!•.: mau cao nhal cua 2 chung vi kh....ir Klcl">iclUi pneumoniae va A,'. '•'.. lla \anicola la 5-". net likng hav g u m pH si> voi gia in nav. hieu qua khu mau gi. ' Mjiilin \ . i c s t 2 0 1 3 ) 1 4 ) trongthi nghiC'm cua minhd.l kl\'i"j dinh. pH mid truong tang tu 5 den ". hieu qua klur mau ting va d.ii hieu qua cao nhal iJi pH ". neu Hep tut i.ing pM loi 9, hiv'u qua khu mau giam. Nghien cuu cua Kumar vj cs (200-)) \\\\ ken Meerbergen va cs |201 M [21 cimg cho kcl qua Urong Ig. hii'u qua khu mau cao nhat cua hon ln,Tp vi sinh v,ii d.ii dui'i khi moi trutHig co gia in pH "-S pli moi Irui'mg anh huong tui hiC'u qua khu mau eua t.ic chung vi sinh v,^i, pli loi uu de loai bo mau nhieu lo^i ihutK nhuom thutmg irong iiguniic tu (> den III |5] Til kel qua nghien cim niiy, co the kel lu.m pli d-" ihieh h(,Tp cho hoat Jong khu mau thuoc nhupm Red I S2H1 cu.i chimg vi khuan V Bing 3. Anh hiramg cua gia tri pH toi kha nang khu mau cua chijng vl khu^n A9.
pli NAngd^thuAc <-'«ir|on Dai chimg Thi ngliifm
HiV'u qua khu mau thuoc nhugm Red FN2B1. CUJ chung
\ i khu.iii Vi eao nhat (Sl,3°o) tai nhiel dg 35 C ihang 4) T^ii nhivt dg 25 v ,i 4ii i hii-u qua loai K) mau thuoc nhuom eua chung V^ khong khac nhau nhicu. Hieu qua khu mau thap nhat [.'4.2"..) khi nhi^-i dg moi iruung la 15 C Kha ning khir mau cua chung v i khuan ihi nghiem giam tai 45 C cb the do giam kha ning sinh truiTng cua le hao hoac bien linh en/ymc chiu trach nhi^-m khu mau lai 45 C. Chung vi khuan .•\9 sinh Inrcmg lol tat 35 C va l3ng cuimg long hgp e n n m e ph.in huv. do do hi?u qua phan huv ihuoc nhugm i.'ine Ki'l qu.i nav cung luimg tu kcl qua nghien cuu cua mpt vo nh.i khoa hgc khac.
Bang 4. Anh hirong cua nhl?t dp tcri kha nang khu mau ciia chung vi khuin A9
Nhividf Noni:dJ G t i t r j O D
, < i l h . i c n h u ^ DoUh^g Thinghifm * ' ' • ' * ' " * ' •
. n i . n v a e s . 2 0 . W , l l 3 ] d n c l l u ^ n J o j M ^ . ^ ^ , ^ ,
„ n h vat loa, bo mau thuoc ' f ' ^ " ' I ' l J j ^ \ i n g len va bj h,,u qua loai bo mau giam ^ ^ ^ % ; . . . . c » . sp. va . u m dang ke lai 45 C L hung v. ^]^^^^-J^^. ^.^ ^ , ( , , 3 ^ j ^ . ] Bacillus -? irong nghien cuu a i a - ^^^. ^.^^ ^^^^j-^ ^ . dai hi^u qua khu '"^'^il-^l'; jjl^'^l^^ ^hii mau gi^m Trong im 45 C hav giam vc _ L UK H ^^. ^^^^^ ^.^
.hi nghi^-m eua Hadi Eslam. va ^l:^ JJ ^ .,^.„/„ ^al . - I- ,,. I i.-.-ali'- va Kiei'Sieiia khuan / . „ k v , , . „ c . » , ' • " ' - (, neu tang nhiel d o toi
ZnTs. khuan ETL-1 va ETL-2 dat hieu qua k h u mau ihuoJnhupm c a . nhdl tai .^v'C va thap nhal la. 4 D ' C ,
4„h hmmg aia ngudn carbon va niia' carbon la nguon nane lu^mg cdn thia cho sinh truong va phat trien ciia vi Mnh v.u va tac dpng nhu chat nhuong dien tu can thiet de he nav eac edu n6i "irong phan ttr thuoc nhugm. Chimg A9 bii'u hiC-n kha niing khu mau t6t nhdt khi nguon carbon la imh hoi (bang 5). lic'p iheo la saccarose \ a lactose, Khi
>u dung ngu6^n carbon la .xylose, hieu qud khir mau ciia chung A9 Thap nhdt (41.6%). Ngudn carbon kliac nhau da anh hutmg icri hieu qua khu mau. Kk qua nay t u o n g tir voi ki-i qua dugc cong b6 bai Palanivelan va cs (2014) [14], Cac ehung vi khudn Bacillus sp (ESL-52), Micrococcus -p (TSL-7) \a Laciohacillus sp. (TS-5) trong nghieti ciru cua Palanivelan bieu hien kha nang khii mau cao nhat khi irong moi irucmg nuoi vi khuan co tinh bgt, cac chiing vi khuan Slapliylococcus sp. (ES-37), Pseudomonas sp. (M- 11 va Bacillus sp. (TSL-9) bieu hien lol da khd nang khii mdu khi nguon carbon Id lactose, Chiing Bacillus sp, trong thi nghiem ciia Pushpa vd cs (2017) [15] khir mau tot nhat khi moi irucmg nuoi co chira tinh hot, nguoc lai maltose va saccarose cho hieu qua khir mau thap.
Bang 5. Anh huffng ciia nguon carbon toi kha nang khu mau ciia chiJng vi khuan A9,
Nguon None do thuoc GiS trj O D nhuqm Doi chung Saccarose
Tmh bpi
Maltose
Thi nghiem o.oyT 0,080 0.151 0.239
><4,5 87,2 75.8 U\ V.
De danh gia anh huong ciia nguon niur den i lia nanq lam mat mdu thuoc nhuom cua chung A9, cae nijuun niio \ 6 c o v a h i J T j c o d a d u o c s u d u n g ( b a n g 6 ) , Ket qumhi riiilnem cho
iKHOAHOC \
thay. nguon niia v6 c o NH.CI va cao nam men giiip chung
\'> khir mdu lol hon cdc nguon nilo khac, my nhien khong h;ing nguon ( N H J , S O j Saad El-Din Hassan vd cs (2015 > [5]
kel lu^n cao nam men \ a N a N O , da lam tang kha nang khir mau thuoc nhupm disperse yellow (D4) ciia chiing v i khuan Klebsiella pneumoniae va Klebsiella variicola Mohan va cs(2013) [12] da ghi nhan peptone la nguon nito thich hgp nhat de chung Planococcus sp. khu mau cua thuoc nhugm Coractive Blue P-3R, nhung cao nam men lai Id nguon nilo ua ihich de khu mau ciia chiing Bacillus sp,
Bing 6, Anh huong ciia nguon nito toi kha nang khir mau ciia Chung vi khuan A9.
Neu^n I None dp
NH.CI (NHJ.SO.
NaNO, KNO, Cao ihil
Pcpliiim
Gi4 Iri OD Doi i
100 mg/l 0,624 nghifm 0,185 0,117 0,434 0,499 0.603 0,302
Kdt luftn
Ba chiing vi khuan A2, A9 \ a A14 co kha nang khir mau dS dugc phdn lap lu mdu nuac thai del nhuom. hieu qud khir mau ctia chiing A9 cao nhat
Hi^u qua khir mau thuoc nhupm Red FN2BL cua chung A9 cao nhdt khi sir dung nguon carbon la tinh bgt. nguon nilo la (NHJ,SO_,. NH^Cl vd cao nam men, nhiet do 35"C va pH ihich hgp trong khoang 6-7. Chiing A9 co tiem nang loai bo mau ciia mrac thai chira thuoc nhugm Red FN2BL tir cac ca so del nhugm.
TM LlEU IHAM KHAO
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