VIETNAM MEDICAL JOURNAL N°2 - APRIL - 2018
21,4%, sau can thiep eung cap khau phan la 32,9%. Ty le ngffdi benh cd mffc Prealbumin thap sau can thiep tff van dinh dffdng la 81,4%, sau khi can thiep cung cap khau phan la 8,6%, sff khac biet cd y nghTa thdng ke vdi p<0,05.
KIEN NGH!
- To ehffc cac Idp tap huan ve cham sdc dinh dffdng cho cac bae sy, dieu dffdng benh vi|n theo cac ehuyen khoa, chit trong cung cap kien thu'c lien quan den tff van, chan doan, dieu tri dinh dudng, tao mdt mdi trffdng tich ct/e trong viec nhin nhan, danh gia vai trd cita dinh dffdng ddi vdi ngffdi benh.
- Can xay dffng va ap dung quy trinh cham sdc dinh dffdng theo tu'ng nhdm benh cu the.
- Xay dffng cac tai lieu truyen thdng dinh dudng cho cac nhdm benh de lam cd sd cho cae can bp y te thffc hien eac ndi dung lien quan den eham sde dinh dffdng ngffdi benh. De nhan rdng md hinh tff van va thffc hanh dinh dffdng cho ea the, can xay dffng cac video hffdng dan thffc hanh
cac che dp an benh ly de lam tai lieu cung cap c{io ngffdi benh, ngffdi nha ngffdi benh thffc hien.
TAI UEU T H A M KHAO
1. E^Yte'(2011).Th6ngtUso08/2011/rr-BYThijem dan conq tsc dmh du8nq, tet che trong benh vien, 2. Bo Y t e ^ Z O l l ) . Thong tU so 07/20il/rr - Bn
hffdng dan cong tac dteu dUSng ve ch3m soc ngudi benh trong benh vien,
3. Bo Y te (2013). Chu'dng trinh^dao tao Cham soc ngVdi benh toan dien
4. Lu'dng Ngoc Khue (2016). Tht/c trang hoat dpng dtnh duSng lam sang, Bao cao Hoi nghi klioa hoc Dtnh dySng iam sang cac tinh phia Bac nam 2016.,' 3. Nguyen Thj Lam (2016). Vai tro cita dinh dii3rK|
dieu tri va cac giai phap cai thien eong tac diSm soc dtiih dudng trong benh vien. Tap chi Dmh DU3ng & Tht/c Pham, 12 (3), 1-4.'
b. Doan Thj Tu'dng Vi, Cao Thj Thu, Du'dng Mai Phu'dng va cong s^i (2016). Hieu qua cita ho ttii dinh dudng trong benh dat thao dUdng tat h^\\
vien 19.8 Bo cdng cart. Tap chi Dinh DuQng & Ttwt Phim, 12 (3), 4-10.
7. P. M. Vegine, A. C. Fernandes, M. R. Torres, et al (2011). Assessment of methods to identify protein-energy wasting in patients on hemodialysis, JBrasNefrol, 33 (1), 55-51.
NGHIEN Ciru MOT SO TAC DONG CO hOl CUA CHE PHAM TRA T H A O MOC TU* LA CHE VA QUA CO
Nguyen Tran Hoang Anh*, Kieu Thanh Hang*
TOIVI TAT
Muc dinh: Xem xet mot so tae dpng c6 ldi den sffc khoe nhu giam beo phi, chong oxy hoa va kha n3ng tieu diet te bao ung thU ciia che pham tra thao mpc tff la, che va qua co. PhUdng phap. So sanh quang pho cua cae dung dich vdt DPPH de danh gia kha n3ng ehong oxy hoa cua CT3. Danh gia kha nang giam beo tren chuot nuoi. Tien hanh phan tich tai phong thi nghiem Khoa Sinh hpc dai hoc SU pham Ha Not. Xac djnh kha tiang khang khuan cita tra thao mgc tren 2 loai vi khuan la Escherichia coli va Salmonella typhimurium. Xac dinh kha nang khang nam M.
cirdnelloides vdt chiing gay benh R7B. Ket qua: La ehe gta co cac vong ffe che la 19,2mm (S.
typhimurium) va 21,3mm ( £ coli), Idn nhat trong cac loaMa, tUcJng dUtJng che pham CT3. La che gta va che pham CT3 co kha nang ffe che sinh tru'dng cita bao tff nam M. araneiloides. Kei luan: Djch chiet ciia tra thao mpc co kha nang khang Escherichia coli va Salmoneiia typhimurium, ffe che manh toe dp stnh trUdng bao tU nam M. cirdnelloides qa^ benh.
^Tru'dng THPT Chuyen Hiing Vu'dng Phu Thg Chiu trach nhiem ehinh: Nguyen Tran Hoang Anh Email: [email protected] Ngay nhan bai: 23.2.2018 Ngay phan bien khoa hpc: 30.3.2018 Ngay duyet bat: 12.4.2018
Tdkhda: khang khuan, khang nam, tra thao moc, la che, qua co
SUMIVIARY
STUDY OF SOME HEALTH BENEFIT OF HERB TEA FROM CAMELLIA SINENSIS AND
LIVISTONA SARIBUS Objective. To evaluate the antibacterial, antifungal properties of herb tea preparations from Camellia sinensis leaves and Livistona saribus fruit.
Companson of antibacterial and fungicidal pnaperties of CT3 formula with old tea leaves and young tea leaves. Method. We had proceeded analytical atthe Biology department of Ha Noi national university of education. Determine the antibacterial of the herbal teas on Escherichia coii and Salmonella typhimun'uffi.
Determine the antifungal on pathogen R78 of M.
ciranelloides Results: The zone of inhibition of old tea leaves were 19.2 mm (S. typhimurium) and 21.3 mm ( £ coli), which was the largest equal CT3. Tlie old tea leaves and CT3 formula could inhibited the growth of M. circinelioides spores. Conclusion: Ttie extracts of herbal teas could resistant to Eschenchia coll and Salmonella typhimurium, which could strongly inhibit the growth of M. circinelioides scores.
Key words: Antibacterial, antifungal, herb tea, camellia sinensis, livistona saribus
124
TAP CHi Y HOC VIET NAM TAP 465 - T H A N G 4 - SO 2 - 2018
I. DAT VAN DE
Mpt so nghien cu'u da cho thay la che va cac chat tinh che tir che c6 kha nang khang khuan, khang virus, chong lao hoa, chong ung thu', tang eu'cJng siTc khoe va keo dai tuoi tho. Trong do, polyphenol dong vai tro quan trpng nhat trong kha nang chong oxy hoa va khang cae loai nam (Yiannakopoulou E. Ch., 2012; Yang X. et ai, 2015; Nguyen Thj Ha va cs, 2005).
Co thupc ho Cau, lau nay qua co van du'cfc sir dung de ehe bien thanh mot vai mon an quen thuoc. Mpt so nghien eifu ve cac loai trong ho Cau cung da cho thay ehiing co chira cae hoat chat eo tiem nang trong viec dieu tn mot so benh d ngiid'i, nhii khang khuan, ung thu', chong oxy hoa, hen suyen, giam beo (Tran Thi Phu'dng Anh, 2008; Tran Van Lpc va cs, 2010).
Tuy nhien, den nay cac nghien eiTu ve hoat tinh sinh hpc cua la che va qua cp trong tren vung doi Phu Tho van con han che. City che hien nay chit yeu du'dc sir dung phan bup non, con la gta van bi coi la phe pham ctja nganh che va gia tri thu'dng mai rat thap. Ben canh do, cac san pliam che ciia Phii Thp hien nay van dang bi ban che ve chiing ioai nen co the cliung ta chua phat huy het gia tri eua cay ehe. Mat khac, nhieu bo phan ciia cay cp chu'a du'dc nghien eij'u va sii dung; qua ep la mot trong nhD'ng dac san eiJa Pliu Thp, nhu'ng no van chi du'dc coi la mot mon an vat ma chu'a du'dc ehe bien thanh nhu'ng san pham eo gia trj cao.
Cau hoi dat ra la: La che va qua ep du'dc trong tai viing doi niii Phu Thp cd nhu'ng hoat ttnh CO \d\ gi doi vdi sire khoe? Neu ket hdp hai dac san nay vdi nhau trong mpt che pham tra thao moc thi cac boat tfnh sinh hpc ciia chijng eo du'p'e gia tang hay khong? Do do, chiing toi da de xuat nghien ciru de tai; "Nghien cu'u mgt sd tic dpng cd ldi cua che pham tri thio mgc tit li che vi qui cg".
W. M%T Ll|U VA PHU'aNG PHAP NGHIEN CU'U 2 . i : Vat lieu
- La che va qua cp: La che (C. sinensis) dddc thu vao thang 6/2016 tai cac viing che sach va quan trpng ciia Phii Thp do la Doan Hiing, Thanh Sdn va Ha Hoa. La ehe du'dc phan chia thanh ba ioai: La non, la banh te va la gia. Can cir de phan ehia eae loai mau la che du'a theo TCVN 2843-79. Qua cp chin (Livistona saribus) dddc thu vao thang 12/2016 tai huyen Cam Khe, tinti Phil Thp.
- Cae nguyen lieu bo sung: mot so nguyen
lieu bo sung vao san pham tra thao moc, gom:
Cam thao, rau ngo va hoa eiic trang.
Bang 1. Ty le thanh phin cua cic cdng thdc ira thio mdc
- - J I g n g thu'c Thanh pR3n-^-^
La che gia say kho Hon hdp bo sung
- Qua CO - Cam thao - Rau ngo - Hoa cue tranq Tong
C T l 2.8 (56%)
2.2 (14%)
0.44 0.66 0.44 0.66 sg
CT2 3.5 (70%)
1.5 (30%)
0.3 0.45 0.3 0.45 5g
CT3 4.0 (80%)
1.0 (20%)
0.2 0.3 0.2 0.3 5g Cac sinh vat du'dc dung de ttiu" nghiem Chuot nhat trang (Mus muscuius) dddc sd dung trong thi nghiem chu'ng mmh vai trd eiia djCh chiet tra thao mpc trong viee phong ngiTa benh beo phi tren ehuot.
Te bao ung thu' vit (MCF7) va te bao tuyen vu lanh benh (HMT3522-S2) cita ngu'di du'dc sir dung de danh gia kha nang tieu diet te bao ung thu'eiia cac loat djch chiet mau.
2.2. Thdi gian va dfa diem nghien cdu Cae thf nghiem va phan tfeh du'dc thuc hien tir thang 6/2016 den thang 2/2017, tai c^c phdng thi nghiem Tru'cfng Trung hpe pho thong Chuyen Hung Vu'dng, Viet Tri va cac phong thf nghiem thuoc Khoa Sinh hpc, Tru'dng Dai hpe Su' pham Ha Noi.
2.3. Phu'dng phap nghien cu'u 2 . 3 . 1 . Phu'dng phap thu' hoat tinh chong oxy hoa
Nguyen tac: Cae ehat co kha nang chong oxy hda se trung hoa goc DPPH bang each cho hydrogen, lam giam dp hap thu tai bu'dc song et/e dat va mau ctJa dung dich phan irng se cliuyen tir tfm sang vang nhat. Trong nghien ciru nay, chat chuan du'dc sir dung la axit ascorbic du'i?c pha bang nu'dc cat khir ion thanh day eac nong dp gom: OmM; O.OSmM; O.lmM; 0.2mM va 0.4mM. Dung djch DPPH diidc pha trong dung moi methanol tinh khiet de dat nong dp O.lmM.
Dieh ehiet eac mau du'dc pha loang thanh day tir 4 den 5 nong dp khac nhau. Du'a vao moi gieng 20pl dieh {chat ehuan hoac dich chiet mau), them isOpl DPPH O.lmM. Cae gieng trong (blank) sir dtJng 20pl nu'dc cat khijr ion bo sung them 180pl ethanol. U trong 30 phut va tien hanh do quang pho tai bu'dc song 517nm, tap lai 3 ian. Kha nang chong oxy hoa ciia dieh chiet du'dc tfnh di/a tren phan tram LTC che (1%). Phan
VIETHAM MEDICAL JOURNAL H°2 • APRIL • 2018 tram u'e ehe (1%) du'dc xac djnh theo cong thu'c
sau (Le Thanh Tam, 2010):
l ( % ) = X 1 0 0 % Ac
Tmng do, Ac: Gia trj mat dp quang cua dung dich khong cd mau thir (control); As: Giajtn mat do quang ciia dung djch cd ehu^ mau thir (sample).
2.3.2. Phu'dng phap thuT Itha nang g i a m b^o t r e n c h u o t beo phi thu'c n g h i e m
Chupt du'dc nuoi trong long nuoi vdi ehe dp chieu s^ng 12h/ngay, nhiet do 25 ± 2''C, dp am 65 ± 10%. De nghien cii'u tac dpng eua djch ehiet ciia tra thao mpc trong viee phong benh beo phi,chiing tdt da tien hanh thir nghiem 3 t i n , moi tan 20 ca the chupt du'dc chia thanh 4 16, mot 16 5 eon, gom:
- Lo binh thu'dng (BT): an khau phan cd sd de tao chupt binh thu'dng;
- Lo beo phi thiTc nghiem (BP): khau phan giau chat beo de tao chupt tjeo phi;
- Lo thi nghiem 1 (BP+1/2C): Chupt du'dc an khau phan giau chat beo va uong djcli chi§t cua che pham tra thao mgc CT3 vdi tteu lu'dng 10 ml/ngay/kg chupt;
- Lo thi nghian 2 (BP4-C): chupt duWc an khau ( ^ n giau chat tieo va uong d[eh ehigt tra thao mgc CT3 vdi lieu luWng 20ml/ngay/kg chupt.
BT BP BP+1/2C BP-l-C Hinhl. Bdt/idc long nudi chupt trong thinghi$m.
BT; Chupt binh thu'dng; BP: Chupt beo phi;
BP+1/2C: Chupt beo phi du'dc bo sung djch chiet pha loang (ty le 1:1); BP+C: Chuot IJeo phi dddc b6 sung djch chiet khong pha loang.
^ Chu^t du'dc cho uong djch chiet 3 lan ngay bang phu'dng phap bdm qua thdc quan, nham dam bao moi ca the chupt trong cung to thf nghiem du'dc bo sung ciing 1 lu'dng dich chtet la che nhu" nhau. Chupt d cac 15 du'dc theo dot khoi lu'dng hang tuan, xac djnh su" thay dot khoi lu'dng sau 3 - 6 tuan thf nghiem.
2.3.3.Phu'dng phap t h i r kha n d n g t i e u diet t e bao ung t h U
Te bao ung thu* vu (MCF7) va te bao lanh (HMT3522-S2) du'dc nuoi trong cac dia 48 gieng trong moi tru'dng thfch hdp. Mot gieng chiJa
6x10'' te bao trong 400|jl mot tru'dng tu'dng iftig, Bo sung dich chiet mau vao cac gieng (doi chu'ng va thire nghiem) theo cae ty le khac nhau (5%, 10%, 20% va 40%). Cac te bao du'dc nuoi bong dteu kien nhiet do 37°C, nong dp CO2 5%. Klia nang diet te bao ung thu' cua djch chiet m§u du'dc xac djnh bang ty le te bao chet sau 24h nuoi cay. De xac dinh te bao chet, chung to nhupm te bao bang dung dich Trypan blue (Sigma), te bao ehet se bj nhupm thanh mau xanh, te bao song khong bj nhupm mau. *.
2.4. Phu'dng p h a p xuT l y so lieu - Cac so lieu deu du'dc xu' ly bang phan mem Microsoft Office Excel 2016. Cac sS lieu trong tgo cao nay deu ^ d c bieu dien du'di dgi^;
^ ± SD, Trong do: ^ la gia trj trung binh, diftlf tinh bang ham AVERAGE. SD la dp lech chuan' (Standard Deviation) du'dc tfnh bang ham STDEV. Sd khac biet thong ke glQla cac nhom (St lieu du'dc danh gta bang kiem dinh T-student (tiam TTEST).
III. K f r QUA VA BAN L U A N
3.1. Khio sit khi ning chdng oxy hoa cua chi'pham
Hoat tfnh chSng oxy hoa la m6t trong nhiitig tfeh chat rat quan trong cua cac che pham, dong vat tro chii dao trong viec phong chong ung thu"
va cac tac dpng cd hai khac ciia eac goe tu" do, Trong nghien cdu, nay, chiing toi tten hanh pha loang eac djch chiet thanh mot day cac do ptia loang: 25, 100, 200 va 400 tan rat tien hanh so sanh vdi chat chuan la axit ascorbic d cac nong dp khac nhau (bang 2 va hinh 2).
Ket qua cho thay: Nhin chung ta che non co kha nang quet goc tu" do DPPH eao hdn so veil la che banh te, la che gia va qua co chin. Dieu nay phu hdp vdi ket qua phan tfch ham lu'dng polyphenol trong eac mau la ehe d phan IV.2.2.
Cd the do sd tich luy cac hoat chat co kha nang chong oxy hoa, dac biet la polyphenol trong la che giam dan theo dp gia a i a nguyen lieu.
- Khi pha loang cac djch chiet mau 400 Kn:
Kha nang quet goc tu" do DPPH ciia tra thao moc CT3 khong khac biet co y nghla thong ke so i ^ eac loai dieh chiet la che va axit ascorbic 0.05 mM (p > 0.05).
- Khi pha loang cac dich chiet mau 200,100 va 25 lan:
Khi eang gtam dp pha loang, che pham &a thao mpc CT3 cang the hien ro kha nang kh^ng, DPPH manh hdn ro ret so vcfi axit ascorbic (0.1, 0.2 va d.4mP4) cac loai djch chiet la che (non^
banh te va gia) va qua cp chfn (p<0.01). Co tl«
126
TAP CHt Y HOC VIET NAM TAP 465 - THANG 4 - SO 2 - 2018 kht nong dp djch ehiet tang ten thi xac suat "bat
gap" cac goc tu" do ciia cac chat chong oxy hoa trong djch chiet cung tang nen kha nang quet eua ehung cung Idn hdn.
Ket qua nay co the do cac thanh phan bo sung trong tra CT3 cung co eae chat chong oxy hoa nen chung lam tang kha nang ehdng oxy
hda ciia che pham. Theo bao cao cua Yao H et al (2012), ioai cp xe (L chinensis), cung hp vdi ioai Co ( i , saribus) trong nghien cdu nay, cd ehu^
nliieu loai flavonoid cd boat tinh chong oxy hoa manh. Tuy nhien, de cd cd sd khoa hoc cu the hdn, eac phan tfeh thanh phan hoa hpc cua Ioal Cp L saribus (^n diidc tien hanh.
Bang 2. 7)rle % ii'c che Chat chuan Axit ascorbic Dich chiet mau
Che pham Ci J Ls non L^ banh te
La qia Qua CO chin
goc tu-do DPPH cua chat chuan va cac dich chiet mau Tv le % ire che DPPH
Omi^
0 BC
0 0 0 0 0
O.OSmM 18.4 ± 2.59"
S400 17.9 ± 13S' 15.4 ± 4 . 5 6 ' 14.3 ± 3 . 8 9 "
13.4 ± 2.37"
10.9 ± 2 . 9 9 "
O.lmM 31.2 ± 2 . 7 8 "
S200 27.8± 2.34""
24.4 ± 2.33"
21.3 ± 2.45"
21.2 ± 3.22"
16.8 ± 1.09"
0.2 mM 53.4 ± 5 . 0 7 "
SlOO 48.7 ±2.56"' 36.6 ± 3 . 2 8 "
35.4 ± 3.22"
31.5 ± 1.22°
27.3 ± 2 . 1 1 "
O.lmM 75.2 ± 6 . 5 0 "
S25 83.5 ± 3 . 4 5 "
68.7 ± 3.44"' 63.5 ± 1 . 2 3 "
59.1 ± 3 . 2 1 ° 52.9 ± 2.30 "
Ghi chu: +Chat ehuan axit ascorbic du'dc pha thanh day nong dp 0; 0.05; 0.1; 0.2 va 0.4 ml^. Djeh chiet mau du'dc pha loang theo cac ty le 25,100, 200 va 400 lan (ky hieu S25, SlOO, S200 va S400).
-1- ^ khae lilet khong co y nghla thong ke so vdi chat chuan (p>0.05); * khae blet co y nghTa thong ke so vdi chat chuan (p<0.05). * * khae biet eo y nghTa thong ke so vdi djeh chiet la che gia (p<0.01)
aaiiti hiiL ii
Hinh 2. So sinh tyl0%dc chigoe iUdo DPPH cua dc loai d/ch chiet vdi chat chuan axit ascorbic Chat chuan axit ascorbic du'dc pha thanh day
nong do 0; 0.05; 0 . 1 ; 0.2 va b.4mM. Cac djch ehiet mau che pham CT3 va la che du'dc pha loang theo cac t^ le 400, 200, 100 va 25 tan (ky hteu S400, S2C0, Sioo va S25).
Mac du la che non co kha nang khang oxy hoa eao hdn so vdi la che gla. Tuy nhien, ham lu'dng polyphenol va caffeine cua la ehe gla tu'dng ling dat tdi 88.2% va 62.3% so vdi la ehe non. Vi vay, la gla nen du'dc xem la mot nguon nguyen lieu quan trpng co the tan dtjng trong viec tach chiet polyphenol va che bien che, thay vi bj coi la phe pham nhu' hien nay.
kha nang quet goc t t ; do DPPH cua che pham tra CT3 cho thay tiem nang cua no trong viec bao ve te bao va cd the trong viec chong lai cac goc t i i do cd hai. Kha nang nay cua tra thao mpc CV^ CO the giup ho t r d cd the chong lai mot so tac nhan nguy hat cho cd the, vf du nhir su* hinh thanh va phat trien cua te bao ung thu*.
3.2. Khio sii khi nang lam giim bio phi cua chepham:Thd nghiem kha nang giam beo phi cua che pham (CT3) dirdc tien hanh tren dot tu'dng chuot nhat trang da du'dc gay beo phi thy'c nghiem. Trong thf nghiem nay, chung toi chi danh gia tac dung cita dieh chiet eua che pham tra thao mpc.
Hinh 3A cho thay su" khac biet ve hinh thai ngoai cua chupt trong cae 16 thf nghiem. Chu6t nuot beo phi eo kfeh thu'dc cd the Idn hdn han so vdi chuot nuoi binh thu'dng. Chupt nuoi beo phi dddc bd sung 1/2 lu'dng djch chiet la che co kfch thu'dc giam mpt chut so vdi chupt beo. Trong khi do, chupt du'dc bo sung djch chiet la che nguyen chat thi cd cd the nho hdn kha ro, mac du van Idn hdn chupt binh thu'dng, song no da cho thay tac dung tfch cu'c cua djch chiet che pham tra CT3 trong viec lam giam kfch thu'dc cd the chupt beo phi. Ket qua nay du'dc khang djnh bdi so lieu ve khoi lu'dng ed the.
VIETNAM MEDICAL JOURNAL N°2 - APRIL - 201S
Banq 3. So sanh khoi iWdno cO the cda cac nhom chuot sau 4 tuan thi nghiem Cathe
1 2 3 4 5 Trunq binh
SD
Nhom BT Otuan
17.8 19.5 18.3 19.8 18.6 18.80 0.83
4 tuan 31.0 31.0 35.0 29.0 31.4 31.48
2.18
Nhom BP 0 tuan
18.5 17.1 21.1 18.0 18.7 18.68
1.49 4 tuan
44.8 39.0 43.0 46.0 43.4 43.24
2.65
N h o m B P + 1 / z c Otuan
17.3 20.7
20.0 18.40
1.94 4 tuan
43.0 35.0
37.4 3.57
0 tuan 20.1 18.5
18.5 1.24
4 tuan 39.S 35.7 36.0 33.0 35.7 35.98
2.31
n I nl ni ni
Hinh 3. So sinh h/nh thii ngoai, khdi IUdng cd thd chudt irong thi nghiem (A) Hinh thii ngoai cua chugt in thu'dng vi an beo. (B) Thay ddi khdi ludng cd the cua chudt
*p < 0.05, **p <0.01, n.s khong cdy nghia thong ke (p > 0.05) Kha nang lam giam khoi lu'dng d chupt beo
phi thi/e nghiem cua dieh chiet che pham tra thao ripe CT3 co the glal thfch d o j a che eo kha nang lam giam hap thu va chuyen hoa llpit d ehuot. Ngoai ra, la che xanh con lam gtam kha nang tieu hoa va hap thij tinh bpt d ngu'di (Lochocka K. et at., 2015). Ket qua nay cho thajf ttem nang cua che pham tra CT3 trong viec ho trd dieu trj beo phi d ngu'di.
3.3. khio sat khi nang diet ti'bao ung thU vu in vitro cua che pham
Mac du y hpc ngay cang phat trien, da co nhieu phu'dng phap tien tien hien dai du'dc ap dung trong chan doan va dieu trj, xong ung thU van la nguyen nhan gay tir vong hang dau doi vdi con ngu&i. Ung thu' vu d phu nO' la mpt trong nhD'ng can benh ung thu" ptio bten nhat. 6 cac nu'dc eong nghiep, tan suat ung thu" vu den
70/100,000 dan/nam. O mien Bac nu'dc ta, tj^ le tir vong do ung thu' vu du'ng hang dBu trong cac loai ung thu" phu khoa (theo Bai giang ung thi;
vu, www.dieutri.vn). Nham danh gla kha ning cita dieh chiet che pham CT3 va cac loai mlu khac trong viee phong chong ung thu", chiing tgt da thir nghiem kha nang tieu diet eae te bao ung ttiu" trong dieu kien in vitro. Sau 24h nuoi cay tt bao ung thu" vu (MCF7) va te bao lapli.
(HMT3522-S2) trong mot tru'dng thfeh hdp c6«b6 sung djch c h t 3 cac mau khac nhau. Ket qua cho thay dieh chiet td che pham CT3 eung nhu' cii qua CO chfn va cac Ioal la che deu eo tae dung gay rai loan sinh tru'dng hole tieu diet te bao ung thu" trong dieu kien In vitro. Trong do, cac Ioal la che deu the hien kha nang tieu diet tS bao ung thu" vu manh hdn ro ret so vdi qua c» chin (hinh 4 v a hinli 5).
Bang 4. Ty le 'yo tebao chet khi bo sung cac loai djch chiet vdi cac nong do khac nliau doS vdi tebao lanh va tebao ung thu'vu
Dona t g b i o HMT3522-S2 1 TvIe
5%
10%
20%
, 40%
fiol chu'nq 2.03±0.21 2.17±0.15 2.73±0.64 2.50±0.17
Qua CO 3.33±0.15 3.63±0.12 12.37±1.1 54.60±1.31
La non 18.87±0.60 46.60±1.18 99.50±0.30 100±0
La banh t e 12.20±0.75 23.67±0.91 57.30±1.30 99.70±0.26
La qia 3.5C±0.1 5.47±0.21 11.43±1.27 99.30±0.46
CT3 ; 3.37±0.31l 5.90±0.26 9.93±0.42 99.57±0.3li
Donq t e bao I^CF7 1 T « l e
5%
10%
20%
40%
Ool chu'nq 5.00±0.87 6.27±0.40 6.13±0.92 5.27±0.40
Qua CO 15.23±0.55 28.43±0.71 44.13±1.59 88.53±1.10
La non 39.13±0.21 74.43±1.40 98.27±1.01 100±0
La banh t e 41.33±0.95 62.77±0.64 85.87±1.63 99.77±0.25
La gia 39.50±0.61 60.30±1.00 82.40±1.15 100±0
CT3_, 45.10±l.ii 64.10±0.82_
89.30±0.Sb 100±0
TAP CHt Y HOC VigT NAM TAP 4G5 - THANG 4 - SO 2 - 2018
non D L J U n h t t B U i l k BQu^cv MCH
Hinh 4. Tyli^/o tebao chit khi bdsung dc loai d/ch chiet vdi dc nong do khac nhau(A}
0& vdi' tSbio linh (HMT3S22-S2). (B) Od/ vdi tebao ung thu vu (MCF7).
\-A bdnfi td
Hinh 5. Ti bio linh (HMT3S22-S2) va te bao ung ihd vu (MCF7) dUdi He dyng cua cic loai dich ch/'et vdi dc ty le khac nhau
Doi vdi t£ bao lanh HrTr3522-S2 (hinh 4A vahinh 5), cac Ioai la che (la non, la banh te va la gia) va che pham Cn deu gay chet gan 100%
khi bo sung 4 0 % djch chigt vao moi tru'dng (bang 4). NhuYig qua cp chi gay chet hdn 50% te bao lanh khi bo sung 4 0 % djch chtet. Tuy nhien, la che non co kha nang gay dpc manh ngay ca
doi vdi te bao lanh, vi no lam chet 100% te tjao khi bo sung 20% - 40% djch ehiet (bang 4). Dpc tfnh nay giam dan doi vdt la che banh te va la che gta. Dieu nay cho thay neu chung ta hap thu mpt lu'dng qua Idn nu'dc tra tiy la non se cd the gay hai cho sure khde.
VIETNAM MEDICAL JOURNAL N'2 - APRIL • 2018 Mat khac, dot vdi che pham CT3 va la che gta
khi b6 sung 5% djch chiet eiia ehiing se khong lam thay doi ty le te bao chet so vdi doi chuTig d te bao lanh. Nhu'ng la che non va la che banh te lai lam tang ty le te bao chet eo y nghTa thdng ke (p < 0.01) so vdi dot chu'ng (do thj hinh 4A).
Doi vdi te bao ung thu" (do thi hinh 5B), khi bo sung 5% dieh chiet, tra thao moc CT3 cd kha nang tieu diet trung binh 4 5 . 1 % te bao ung thu^, manh hdn ro ret so vdi cac loai djch ehiet la ehe (p<0.01) (bang 4 ). Khi bo sung 10% va 20%
dich ehiet, tra thao mpc CT3 van duy tri kha nbng diet te bao ung thd vu manh hdn so vdi la che gia va la ehe banh te, khi nd eo kha nang lam chet tdi 6 4 . 1 % va 89.30% te bao ung thd In vitro (bang 4). r^ac du eac b/ le gay ehet nay thap hdn so vdi la che non d eiing nong dd. Tuy nhien, ddi ehieu vdi do thi hinh ISA, vdi eac t / le 10% va 20%, djch ehiet la che non va la che banh te lat gay hai Idn doi vdi ea te bao lanh.
Dieu nay eho thay la che non va la ehe banh te khong nen du'dc sir dung vdi ty le cao qua 10%.
Do do, la che gia thfeti hdp nhat khi bo sung vao che pham tra CT3.
Cac phan tfeh trSn cho thay djch ehiet cua tra thao mpc CT3, la che gla va qua cp chin d nong dp < 10% khong gay dpc doi vdi te bao lanh nliu'ng eo kha nang gay ddc manh dot vdi te bao ung thu' In vitro. Dieu thu vi la djch chiet tCf' tra thao mpc eong thii'e Cr3 co kha nang diet te bao ung thir vit manh hdn c6 y nghia thong ke (p<0.01) so vdi la che gia va qua cp chin khi bo sung 5%, 10% va 20% djch chiei (hinh 4B).
Dieu do cho thay st/ ket hdp cua la che gia, qua cp va cae thanh phan phu gia viTa giup gia tang kha nang khang te bao ung thu" vira giam thieu kha nang gay ton hai te bao lanh benh ciia che pham Cr3. Ket qua nay cd the giai thfch dtJ^
tren cd sd phan tfch ham lu'dng polyphenol tong so eiJa tra Cr3 dddc gia tang so vdi la ehe do sd CO mat ciJa cac thanh phan bo sung. Cac thanh phan polyphenol bao gom ca flavonoid, catechin, caffeine va tannin trong tra CT3 da gop phan bao ve te bao lanh va dc che, tieu diet te bao ung thu'. Tuy nhien, ket qua nay mdi chi tien hanh tren te bao In vitro, nen chung tdi chu'a eo dii ed sd de ket luan ve lieu diing doi vdi ngu'di.
Nhu" vay, nhu'ng ket qua nghien cu'u da trinh bay d tren cho thay che pham tra thao mpc C H vu^ dddc danh gia tot ve mat cam quan, lai v i i ^ the hien du'dc mot so tac dpng tfch ei/c, nhu" kha nang khang khuan, khang nam, chong oxy hoa, giam beo phi va gay dpc dot vdi te bao ung thu"
vii In vitro. NhD'ng ket qua bu'dc dau nay md ra
trien vpng ve vtec tJlig dung ehe pham tra tliao mpc c t 3 cd sti" ket hdp ciia la etie gia, qua co chin va mpt so thanh phan bo sung khac (cam thao, rau ngo, hoa ciic tran_p) nhu* mpt thir iK uong, dong thdi gdp phan ho trd dieu trj mot so loai benh d ngu'di.
V. KET LUAN
Kha nang ehong oxy hda ciia ehe pham tra thao mpc CT3 d dp pha loang du'di 200 lan manh hdn ro ret so vdi chat chuan axit ascorbic va cac Ioal mau la che va qua cp chin. Ben eanh do, djch chiet ciia ehe pham tra thao mpc CT3 co tac diing lam giam kich thu'dc va khoi lu'dng ccf the eiia ehuot beo phi thu'c nghiem.
Che pham tra thao mpc CT3, la ehe gia va qua CO chin d nong dp < 10% khdng gay chSt doi vdi te bao lanh nhirng cd kha nang gay cl5c manh doi vdi te bao ung thu' In vitro. Sii ket hijp ciia la ehe gia, qua cp va cac thanh phan ktiac trong tra thao mpc c f 3 lam tang kha nang tieu diet te bao ung thu* vu ciia che pham.
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