Nguyen Thi Kim Lan vd Dtg Tgp chi KHOA HOC & CONG NGHE
S i r L i r u H A N H V I R U S G A Y B E N H T A I X A N H V A K E T Q U A P H A N L A P V I K H U A N G A Y V I E M P H O I 6 L O N T A I T I N H T U Y E N Q U A N G
Nguyin Thi Kim L a n ' , Nguyin Van Quang, Nguyen Thi Ngan, Tran Nhait T h i n g , Nguyen Huu Hoa Trucmg Dgi hpc Nong Ldm - DH Thdi Nguyen
T O M T A T
Ket qua nghien ciiu sy luu hanh virus gay benh Tai xanh 6 Ign tai tinh Tuyen Quang cho thay: Co 22% so miu huyet thanh Ign dugc kigm tra duong tinh vai virus PRRS. Ty Ie mau huyet thanh duong tinh d Ign tai thanh pho Tuygn Quang cao nhit (42%). Trong do, Ign con co ty le huyet thanh duong tinh vdi virus PRRS la cao nhit (37,5%), thip nhat la Ign nai (9,09%); ty le Ion co huygt thanh duong tinh d vp Dong - Xuin cao ban so vdi vp He - Thu ( 27,5% so vdi 15,5%).
Ngoai ra, khi tien hanh phan lip vi khuan gay vigm phoi a Ign thay c6 7,25% so mau phoi va cuong hpng ciia Ign phan lap duoc vi khuin A. pleuropneumoniae; 11,5% so mau phan lap dugc vi khuan P multocida va 13,00% so mlu phan lap duoc vi khuan S. suis. CJ Ign duang tinh va am tinh vdi PRRSV dlu phan lap dugc 3 loai vi khuan tren,
Tir khda: Am linh, duang linh, huyet thanh, Ign, virus PRRS, viim phoi, vi khudn D A T V A N D E
Tir n i m 2007 - 2013, b | n h Tai xanh da biing phat mgnh d nhieu tinh, thanh trong ca n u d e . L i n d i u lien djch Ign Tai xanh bimg p h i t d H i i D u o n g vao ngay 12/3/2007, sau do dich lay lan nhanh va rdng khap c i c tinh mien B i c ( C u H & i P h i i , 2 0 l i ) [ 5 ] .
Tinh dgn t h i n g 10/2016, linh Tuygn Quang cd tdng sd 518 nghin con Ign. M a c du linh Tuygn Quang chua cd dich benh Tai xanh x i y ra, song d cac linh lan can da cd dich, vi vay Ign nudi tai Tuyen Quang v l n cd nguy c o n h i l m virus P R R S , Nhieu lac gia d i nghien cuu v i cho t h i y , khi Ign m i c PRRS t h u d n g bi c h i t do viem phdi ke phat bdi mgt sd loai vi khuan trong dd cd Actinobacillus pleuropneumoniae (A.pleuropneumoniae), Pasteurella multocida (P.multocida).
Streptococcus suis {S.suis) (Biii Quang Anh va cs, 2008 [1]; Jantafong T. v i cs, 2015 [8]) Tir nhihig v i n d l tren cho t h i y , viec nghien cim su luu hanh virus gay bpnh va p h i n lap c i c vi khuan cd kha nang g i y viem phdi kg phat, tir do cd bien p h i p giam s i l , phdng chdng benh Tai xanh d Ign la viec lam rat c i n thilt. Vi vgy, trong n i m 2 0 1 6 - 2 0 1 7 , chiing tdi d i nghien ciiu v l su luu hanh virus gay
Tel 0912 660317, EmaiL lanquangi 9@gmad com
benh Tai xanh v i phan l i p vi khuan gay viera phoi d Ign tai tinh Tuyen Quang,
V A T LIEU, N O I D U N G V A PHU'ONG P H A P N G H I E N CUTJ
Vdt lieu
- Lgn cac liia tudi nudi d 4 huyen, t h i n h phd ciia linh Tuyen Quang. Nhiing lgn n i y chua d u g c tiera vaccine phdng benh Tai xanh, - M a u huyet thanh ciia lgn: 400 m i u . M a u phdi, cudng hgng cua Ign: 400 mau - T l bao M A R C - 1 4 5
- Cac logi radi trudng dimg de nudi c i y , phan lap VI khuan do hang Oxoid (Anh) v i Merck (Phip) s i n xuat i ± u mdi trudng nude thit, thach thudng, thgch mau, thach MacConkey, thach Chocolate...Cac hda c h i t v i dung cu thi nghiem khac.
Ngi dung nghien cdn
- N g h i e n ciiu su luu h a n h virus g i y b e n h Tai x a n h d lgn tai 4 h u y p n „ t h i n h ciia tinh T u y e n Quang.
- Phan lap mdt sd vi khuan thudng gay viem phdi ke p h i t trong benh Tai xanh tir phdi va cudng hgng cua Ign tai Tuygn Quang.
Phuffngphdp nghien cuu Chon mdu dieu tra:
- Chgn mau theo p h u o n g phap ngau nhien, l i y mau chum nhieu bgc.
119
Nguyin Thi Kim Lan vd Dig Tap chi KHOA HOC & CONG NOHb 10B(,U8;: I I V - lin
• Cic logi miu dugc lay ngau nhien tren nhiing Ign chua dugc tiem vaccine phdng benh Tai xanh, chua dugc su dung thudc khang sinh dieu tn.
Phuffngphdp lay mdu:
- Lay mau mau ciia Ign d vinh tmh mach cd, de mau ddng va chat huyet thanh.
- Dimg dao md vd trimg cit liy thiiy phdi va cudng hgng cd benh tich viem phdi ciia Ign (ddi vdi Ign bi viem phdi) hoac cit thuy phdi chua cd bpnh lich (doi vdi Ign khdng bi viem phdi).
- Cac loai mau benh phim dugc bio quan trong dieu kipn vo trimg, d 4° C.
Phucmg phdp ditng phdn ung IPMA (Immunoperoxidase monolayer assay) + Sii dung phin ling IPMA dg phat hipn khang the khang virus PRRS, ggi la ky thuit mien dich peroxidase mdt lap. Ky thugt nay dugc Ihuc hien tren cac ddng te bio MARC - 145. Te bao sau khi nudi cay 24h se dugc giy nhigm vdi virus PRRS vi dugc li d 37" C trong 24h.
+ Sau khi gay nhiem, cac tieu ban niy se dugc cd dinh va lac dung vdi mau huyet thanh, ii khoing 1 gid d 37° C, sau do tiep tuc
Bing 1. Slf luu hdnh virus gdy binh lai xanh
cho tac dung vdi khang khing the lgn cong hgp HRPO (Horseradish peroxidase).
+ Kgt qui: Phan iing duong tinh khi mlu huyet thanh cd chiia khang the khing virus PRRS cd 30 - 50% sd tl bao cd mau do khi cho Idp le bao tac dung vdi dung dich chromogen trong 30 phiit. Phan iing im tinh khi khdng cd su thay ddi man cua te bio.
Phuffngphdp nuoi cdy, phin lap vi giam dinh vi khuan A. pleuropneumoniae, P. multocida vi S. suis:
Nudi cay, phin lip va giim dinh vi khuan A.
pleuropneumoniae, P. multocida va S. suis theo cac quy trinh thudng qui ciia Bg mon vi triing - Vien thu y Qudc gia (Cu Hihi Phii, 2011) [5].
Phuffngphdp xif ly so lieu: Sd lieu dugc xir ly theo cac cdng thiic toan hgc tren phin mera Excel 2007.
Dia diem phdn tich mdu: Phdng thi nghiem ciia
Tnmg tim chin doan Ihii y trung uong, Vien thli y, Trudng Dai hgc Nong Lam Thai Nguyen KET QUA NGHIEN CUU VA THAO LUAN Su luu hinh virus giy benh Tai xanh d Ign tai 4 huyen, thanh phd ciia linh Tuyen Quang.
& tai 4 huypn, thdnh cua linh Tuyin Quang Bia diem So Ign xet nghiam (con) So lgn dmmg tinli (con)
Tvle(%)
TP. Tuyen QuangH. ygn Son H. Chiem Hoa H. Son Duong Tinh chung
100 100 100 400
42,00 12,00 21,00 13,00 22,00
Bang 1 cho thay: Kiem tra 400 miu huyet thanh tai 4 huyen, thanh phd ciia linh Tuygn Quang thiy: ty le miu duong tinh la 22%., biln ddng tir 12 - 42%.. CJ cic huypn, thinh phd phat hien thay lgn mang virus PRRS vdi ly le khac nhau, ly le cao nhat thay d TP. Tuyen Quang, sau do la huyen Chigm Hda, ty le thip nhil la huypn Ygn Son vi Son Duong.
Dieu dd cd nghia li nguy co biing phat dich bpnh Tai xanh la cd the xay ra d tinh Tuygn Quang, neu khdng cd bien phap giam sat va phdng chdng thudng xuyen va hieu qui.
Su luu hdnh virus gdy benh tai xanh & Iffn theo lua tudi
Bang 2. Su hm hdnh virus gdy binh Tai xanh & Iffn theo lira tuoi LqgiIgn So Ign xet nghiem (con) So lgn diroTig tinh (con) T y l f ( % ) Lon con (< 1,5 thing tuoi)
Lgn sau cai sira (>1,5 - 3 thing hi6i) Lgn thjt (>3 - 8 thing mli) Lgn nai
Tinh chung
14 326
6 4 74
4 88
37,50 28,57 22,69 9,09 22,00
120
Nguyin Thi Kim Lan vdDtg Tap cU KHOA HOC & CONG NGHE 168(08). 119-124
TJlg(%) ^ 30 20 10 0
1 •
S 1,5 thing
m
1 m
>1,5 - 3 thang
fl m
>3 - 8 thjng Ndi
Hinh I • Biiu do su luu hdnh virus gdy binh tai xanh theo lua tuoi Iffn Bang 3. iSir liru hdnh virus gdy binh tai xanh theo mua vy Mtia vy
Dong -Xuan He - Thu
Tmh chung
So lon x6t nghiim (con) 200 200 400
So Ion dirong tinh (con) 55 33 88
T * i | ( % ) 27,50 16,50 22,00
Kgl qui d bang 2 va hinh 1 cho thiy: Cac loai
lgn khac nhau cd ty le huygt thanh ducmg tinh vdi virus PRRS khic nhau. Lgn con < 1,5 thing tudi cd ty le huyet thanh duong tinh vdi virus PRRS la cao nhat (37,5%), sau dd den lgn sau cai siia (28,57%). CJ lgn thit, Ign nii va hau bj ty le huyet thanh duong tinh vdi vims PRRS thap hem lgn con: Iin lugt la 22,69% va 9,09%.
Nguyen nhin lgn con dudi < 1,5 thing tudi va Ign sau cai sGa cd ty le huyet thanh ducmg tinh vdi virus PRRS cao la do he thong thin kinh chua hoan chinh, thich nghi vdi ngoai cinh chua tdt, din tdi sue de khing kem, Dong thdi virus PRRS cd tinh dinh hudng te bao cao nhu tg bao phe mang nen virus PRRS de xam nhap va ton t^i trong co thg Ign con.
Su luu hdnh virus gdy bpnh tai xanh theo mua vu
K6t qui xac dinh su luu hanh virus giy benh Tai xanh d lgn nudi trong cac mua vg khic nhau dugc trinh bay d bang 3.
Kgt qua bang 3 cho thiy: Ty 1? lgn cd huygt thanh duong tinh vdi virus PRRS tai tinh Tuygn Quang trong 2 mua vy la khac nhau: d vu Ddng - Xuan la 27,5%, vg He - Thu la 16,50%,
Sd dl cd sg khac nhau vg ty lp luu hanh virus PRRS giiia hai miia vu la do: Trong vy Ddng - Xuan cd nhiet do Irung binh va sd gid nang
thip nhat trong nam, die bipt vio thing 12 va thing I (nhipt do dao ddng tii I5,5°Cdgn 16°
C). Thdi gian nay cd nh&ig dgt rgt keo dai, kem theo mua phiin, do am khdng khi cao, lanh va am la nhfing dieu kipn bat Igi cho 1cm, nhit la Ign con theo me. Vi vay, d vy Ddng - Xuin Ign de nhiem virus PRRS hon so vdi vy He - Thu.
Ket qud phdn lap vi khuan A.
pleuropneumoniae, P. multocida vd S. suis &
phoi vd cudng hgng lp7t t^i cdc dia phuffng cua tinh Tuyen Quang
Chiing tdi da tien hinh phan lap vi khuan A.
pleuropneumoniae, P. multocida va S. suis d cac mau benh phim ciia Ign thu th^p tai Tuyen Quang. Kgt qui dugc trinh biy d bang 4.
Kgt qui d bang 4 cho thiy: Tir cac miu phdi va cuong hgng ciia Ign thu thap lai 4 huyen, thinh phd thudc tinh Tuygn Quang deu phin lap dugc 3 lo^ vi khuin A.
pleuropneumoniae, P. multocida va S. suis vdi ty Ip phin lap tucmg iing la 7,25%.;
Il,50%val3%,
Mat khac, vi khuan A. pleuropneumoniae trong nghign ciiu ciia chiing tdi cao hon so vdi ket qua nghien ciiru cua Trinh Quang Hipp va cs. (2004) [3], lic gia da phan lap dugc vi khuin A pleuropneumoniae tir phdi va hach lympho ciia lgn vdi ty Ip la 3,33%), til' dich ngoay mCi la 6,8%.
121
Nguyin Thi Kim Lan vd Dtg Tap chi KHOA HQC & CONG NGHE 168(UB): I i y - i 2 4 Ket qua phan I$p vi khuan P. multocida cua
chimg tdi t h i p hon so vdi ket qua nghign ciiu cua Cu Hthi Phli (2011) [5]. T i c g i i cho bigt:
ty lp p h i n lap d u g c vi khuan P. multocida d 90 m l u benh p h i m Ign mac benh tai c i c d dich PRRS l i 14,44%. Theo nhigu lac gia (Albina E. va cs 1994 [6], Thomas Blaha va cs 2005 [9], Cii Hiiu Phii va cs, 2006 [4], L e Bang 4. Kit qud phdn lgp vi khudn A. pleuropneumoi
Van D u o n g 2013 [2]) thi vi khuan nay co vai Ird rat quan trgng trong nhiing ca bpnh viem phdi d Ign, d | c biet la khi Ign mac bgnh Tai xanh thi vi k h u i n S. suis l i mOt trong nhirng nguygn nhan chinh lam cho lgn chet nhigu trong cac d dich tai xanh tai cac dia phucmg trgn c i nude trong nhiing nam vira qua.
'-, P. multocida vd S. s lgi tinh Tuyin Quang
a phoi vd cuong hgng lgn
Dja phuong (huypn, thanh)
Ket qua phan l | p vi khuan So mau
p h i n lap
A. pleuropneumoniae P. multocida So mau (+) Tylg
(%)
S6 mSu (+) T y l g SomSu TJIS (%)' (+) (%) TP. Tuyen Quang 100H. Yen Son z H. Chiem Hoa 100 H. Son Duong 100
Tinh chung 400 29
10,00 5,00 6,00 8,00 7,25
19 8 10 9 46
19,00 8,00 10,00 9,00 11,50
20 10 8 14 52
20,00 10,00 8,00 14,00 13,00 Ket qud phdn lap vi khuan A. pleuropneumoniae, P. multocida vd S. suis O" Iffn cdc lira tudi De l i m ro vai trd g i y b^nh ciia vi k h u i n A. pleuropneumoniae, P. multocida va S. suis ddi vdi lgn d c i c Iiia tudi k h i c nhau, chiing tdi d i p h i n l i p 3 loai vi khuan tren d cac mau phdi va cuong hgng ciia lgn theo tudi. Ket q u i dugc trinh b i y d b i n g 5.
Bang S. Kit qud phdn lpp vi khudn A. pleuropneumoniae, P. Multocida vd S. suis & lgn cdc liia tuoi Lo^iIon
< 1,5 thang
> l , 5 - 3 t h a n |
> 3 - 8 thdng Lgrn ndi Tinh chuns
So mau — KT 16 . 14
326 44 400
A. pleuropneumoniae S o m a n
(+) 1 4 20 4 29
T J l c (V.) 6,25 28,57 6,13 9,09 7,25
P. multocida So mau
(+) 1 4 36 5 46 Bang 6, Ket gad phdn lap 3 Ioai vi Ichuan & itm duong tinh vd dm Xet nghiam
huyit thanh Duong tinh vol PRRSV
Am tinh voi PRRSV
TuSi Ion
< 1,5 thang 1,5-3 thang
> 3 - 8 thing Nai T6ng
< 1,5 thdng 1,5-3 thang
> 3 - 8 thang Nai Tong Tinh Chung
So kiem - 6 4 74 4 88 10 10 252 40 312 400
A, pleuropin So mau
(+;
1 2 6 0 9 0 2 14 4 20 29
zumoniae T J I f
(%>
16,67 50,00 8,10 0 10,22
0 20,00
5,55 10,00 6,41 7,25
TylS
(%)
6,25 28,57 11,04 11,36
i:,so
tinh vCT vir So mau
3 2 39 8 52 m PRRS P. multocida So mau
f+) 1 1 6 1 9 0 3 30 4 37 46
T J l ? S
(%)
16,67 25,00 8,10 25,00 10,22 0 30,00 11,90 10,00 11,85 11,50
S. suis (+)T).1?(%)
S.
18,75 14,28 11,96 18,18 13,00 suis io mau Ty 1$
(+) 1 1 8 1 11 2 1 31 7 41 52
16,67 1%) 25,00 10,80 25,00 12,50 20,00 10,00 12,30 17,50 13,14 13,00 Qua bang 5 cho thay:
Cac mau phdi va cuong hgng cua lgn d 4 nhdm tudi deu phan pleuropneumoniae, P. multocida v i S. suis. Trong do, sd m i u p h i n lg]
122
ap d u g c vi khuan A.
dugc vi khuan S. suis
Nguyen Thj Kim Lan va Dtg Tgp chi KHOA HOC & CONG NGHE 168(08): 119-124
chigm ty le cao nhil; sau dd dgn vi khuin P.
multocida vi thip nhit li vi khuin A.
pleuropneumoniae.
Cu the, ty Ie phan lap dugc ddi vdi tirng Ioai vi khuan d mdi Iiia tudi nhu sau:
*Vi khuin A. pleuropneumoniae:
Ty Ip miu phin lap dugc vi khuin A.
pleuropneumoniae ciia lgn 1,5 - 3 thing ludi cao nhit, chigm ty le 28,57%. Lgn d cac lua tudi khac vi lgn nai nhigm vi khuan nay it hon (bign dgng tir 6,13 - 9,09%),
* Vi khuin P. multocida
Ty le mau phan lip dugc vi khuin P.
multocida cua lgn 1,5 - 3 thing tudi li cao nhil, chiem ty le 28,57%o. Lgn d cac liia tudi khac va Ign nai ty Ip nay thip hon (6,13 - 11,36%).
* Vi khuin S. suis
Lgn < 1,5 thing tudi cd 3 miu phan lip dugc vi khuin S. suis chigm ty lp 18,57%. Lgn d cac Ilia tuoi khic va Ign nai ty lp mau phan lip dugc vi khuan niy li 11,96 - 18,18%..
Ket qua phin lap 3 l o ^ vi khuan d lgn duong tinh va am tinh vdi virus PRRS
Kgt qua phin lap 3 loai vi khuan d Ign duong tinh va am tinh vdi virus PRRS dugc trinh bay d bing 6.
Ket qui bang 6 cho thay:
- Doi vdi Ign duong tinh vdi PRRSV; Trong 88 mau benh pham ciia Ign cac liia tudi duong tinh vdi PRRSV cd 10,22% so miu phan lap dugc vi khuan A. pleuropneumonia; 10,22%
so mau phan lap dugc vi khuin P. multocida;
12,5% sd mau phan lip dugc vi khuin S. suis.
- Ddi vdi lgn am tinh vdi PRRSV: Trong 312 mau benh pham ciia lgn cac liia tudi im tinh vdi virus PRRS cd 6,41% sd mau phin lap dugc vi khuan A. pleuropneumonia; 11,85% sd mau phan lap dugc vi khuan P. multocida;
13,14%. sd mau phan lap dugc vi khuin S suis.
Vdi thuc trang niy, khi Ign bi mac benh Tai xanh, siic de khing ciia Ign giam siit thi cac vi khuin nay se sinh sdi, gay viem phdi kg phat
va lam chet lgn. Nhiing lgn khdng mac PRRS Cling de bi benh viem phdi khi siic de khang suy giara. Vi viy, can phat hien va dieu trj kjp thdi benh viem phdi cho lgn bang cac phac do digu tri hieu qua de giam thiet hai do benh gay ra tren dan Ign.
Kit qud gidm dinh dac tinh sinh vat, hda hpc cua cdc chung vi khuan phan lap duprc Chiing tdi da giim dinh mdt sd die tinh sinh v^t, hda hpc ciia cac chiing vi khuan phan lap dugc d cac miu phdi va cudng hpng ciia Ign tai tinh Tuyen Quang. Ket qua cho thiy cac chiing vi khuin A. pleuropneumoniae, P.
multocida vi S. suis phan lip dugc tix lgn d tinh Tuyen Quang qua giam dinh dgu cd die tinh sinh vit, hda hgc phu hgp voi cac die tinh ciia cac loai vi khuin nay ma Barrow G.I.
va Feltham R.K.A. (2003) [7] di md ti.
KET LUAN
- Cd 22%. so mau huygt thanh lgn thu thip tai tinh Tuygn Quang duong tinh vdi virus PRRS.
- Lgn con dudi 1,5 thing tudi cd ty le huygt thanh duong tinh vdi virus PRRS li cao nhat (37,5%), thip nhit la Ign nai (9,09%).
- Ty lg Ign nudi d vu Ddng - Xuan cd huygt thanh ducmg tinh vdi virus PRRS cao hon so vdl vu He - Thu (27,5% so vdi 15,5%).
- Cd 7,25%) sd mau phdi vi cudng hgng cua lgn thu thsip lai Tuygn Quang phin lip dugc vi khuin^, pleuropneumoniae; 11,5% sd mau phan lap dugc vi khuan P. multocida va 13%
sd mau phan lap dugc vi khuan S. suis.
- (3 Ign duong tinh va im tinh vdi PRRSV dgu phan lap dugc 3 Ioai vi khuin tren.
- Cac chimg vi khuin A, pleuropneumoniae, P. multocida va S. suis phin lap dugc tir lgn d tinh Tuyen Quang qua giam dinh deu cd dac tinh sinh v§t, hda hgc phii hgp vdi cac dac tinh chuin ciia cac loai vi khuan nay
TAI LIEU THAM KHAO
1. Dili Quang Anh, Hoang Van Nam, Van Dang
Ky, Nguyen Van Long, Nguyen Ngpc Tien (2008), Hpi chung roi logn ho hdp vd sinh sdn 123Nguyin Thi Kim Lan va Dtg Tgp chi KHOA HQC & CUNO NOiiii itioiuo;: 124 (B4nh Tai xanh), Nxb Nong nghiep, Ha Noi, tr.
7 - 2 1 .
2. Le Van Duong (2013), Nghien cihi mpt so dgc tinh sinh hgc ciia vi khudn Actinobacillus pleuropneumoniae, Pasteurella multocida.
Streptococcus suis gdy viim phoi trong Hpi chiing roi logn ho hdp vd sinh sdn & Iffn tgi Bdc Giang, biin phdp phong trj, Lugn an tign sT Nong nghiep, Tnrcmg Dai hoc Nong Lam - DH Thai Nguyen.
3. Trinh Quang Hiep, Cu Hihi Phii, Nguyen Thu Hang, Au Xuan Tuan (2004), "Xac dinh d|c tinh sinh vit hoa hpc, doc Igc cila vi khuan Actinobacillus, Pasteurella va Streptocococcus gay bgnh viem phgi 6 lgn". Tap chi Nong nghiip vd PTNT, (4), tr. 476 - 477.
4. Cii Hihi Phli, Nguyin Ngpc Nhien, Nguyen Thu Hing, Au Xuin Tuin, Nguyin Bich Thuy, Vii Ngpc Qu^, Pham Bao Ngpc (2006), "Xac dinh nguyen nhan gay bgnh dudng hd hap ciia lgn nuoi tai m^t so tinh phia Bac", Tgp chi Khog hgc ky thudt thuy, 12 (4), tr. 23 - 3 2 , '
5. Cii Hihi Phli (2011), "Nghien ciiu mdi IJgn quan giua Hpi chiing r6i loan ho hap va sinh san o Ion
vdi vi khuan gay benh ke phat va xac dinh bi|i,.
phap phong, tri bpnh", Bdo edo khoa hoc Vipn Thi y Quoc gia.
6. Albina E., Madec F., Cariolel R., Tomson J, (1994), "Immune response and persistence of the porcine reproductive and respiratory syndrome virus in infected pigs and farm units". Vet. Rec, 134, pp, 5 6 7 - 5 7 3
7. Barrow G, I,, Feltham R. K. A. (2003), Cowan and Steel's manual for the identification of medical bacteria, third edition, Cambridge University Press, UK,
8. Jantafong T., Sangtong P., Saenglub W., Mungkundar C , Romlamduan N., Lekchareonsuk C , Lekcharoensuk P. (2015), "Genetic diversity of porcine reproductive and respiratory syndrome virus in Thailand and Southeast Asia from 2008 to 2013", Vet. Microbiol. 176 (3 - 4), pp. 229 - 238, 9. Thomas Blaha, Robert B. Morrison, Thomas Molitor, Gert, Wensvoort (2005), "Update on porcine reproductive and respiratoiy syndrome (PRRS)", S-ivine Health and Production, Vol 3.
No. 6.
S U M M A R Y
T H E C I R C U L A T I O N OF PRRS VIRUS A N D B A C T E R I A L I S O L A T E D RESULTS C A U S E D P N E U M O N I A IN PIGS IN T U Y E N Q U A N G P R O V I N C E
Nguyen Thi Kim Lan', Nguyen Van Quang, Nguyen Thi Ngan, Tran Nhat Thang, Nguyen Huu Hoa University of Agriculture and Forestry - TNU
The result of prevalance of PRRSV (porcine respiratory and reproductive syndrome virus) in pigs at Tuyen Quang province showed that; there was 22% of pig serum samples were tested positive with PRRS virus. The seroprevalence In pigs at Tuyen Quang city was highest (42%). In these, piglets have the highest seroprevalence with PRRS vims (37.5%), the lowest were sows (9.09%);
the prevalence of seropositive pigs in winter-spring season was higher than in summer-autumn season (27.5% in comperation with 15,5%). In addition, there were isolated bacteria which caused the pneumonia in pigs showed that A. pleuropneumoniae bacteria were found in 7.25% of lung and throat samples; 11,5% of samples were isolated from P. multocida and 13.00% were isolated from S. suis In both positive and negative pigs with PRRS virus were isolated three mentioned bacteria, Keywords; negative, positive, serum, pigs, PRRS virus, pneumonia, bacteria
Ngdy nhgn bdt: 16/6/2017; Ngay phdn bipn:21/6/2017; Ngdy duyft ddng: 31/7/2017
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