KHOA HQC C 6 N 0 MOHt
MGHlilV CLIIJ OA DAIMG Dl TRUY£I\I T A P DOAIM LUA C H A T LUpniG BAIM 0 | A CUA VlffT IMAM BAIVG CHi TH|
PHAIM TLT SSR VI VC TIIVH (MICROSATELLITE)
Khuit HOu Tiung', Nguyfn Thi ly", DJng TTii Tlianh H i ' , Nguyfn Minh Anh Tliin'
T6MTAT
Sii dung 31 chl thi SSR df nghien cihi da dung di truyf n lip doin Ida chit lupng bin dia ciia Vift Nam vi di ttiu dupc tdng sd 1.519 bang ADN, thufc 120 loai alen khic nhau (vdl hf sd trung binh 3,87 alen trtn mdl locus). Hf sd PIC dao d(ng Hi 0 dfn 0,74 (bimg binh 0,52). Tl If d| hpp tii cua cic gidng Ilia chit lupng khic nhau, dao ddng tit 0% dfn 14,29% (trung binh 2,32%). Hf sd tuong ddng di buydn gifla cic gidng dao d^ng trong khoing tii 0,11 dfn 0,87. Dva vio so dd mfl quan hf dl tmyfn 50 gidng hia nghidn ciiu dupc chia tiiinh 6 nhdm ldn: Nhdm 1 gdm cd 23 gidng liia c6 bf sd hiong ddng dl truyf n dao df ng tir 0,45 dfn 0,87;
nhdm 11 gdm c6 10 gidng lua c6 hf sd tuong ddng dl truyf n dao df ng tir 0,45 dfn 0,74; nhdm 111 gdm 2 gidng Ilia cd hf sd tuong ddng di truyfn 0,49; nhdm IV gdm duy nhit mft gidng lua; nhdm V gdm cd 2 gidng Ilia cd hf sd tuong ddng di tiuyf n 0,41. Nhdm VI gdm cd 12 gidng Ida cd hf sd tuong ddng di truydn dao df ng tir 0,43 dfn 0,81. Kft qui niy lilm co sd df phin loai, xic d|nh marker. nhJn dang chinh xic mft sd ngudn gien phvc vv cdng tic bio tdn vi chpn, tao gidng liia chit lupng cua Vift Nam
Tir khda- Chi tly SSR, da dang di truyin, ddng hop, dihqp, gidng lua chit lucmg.
LBlTVliNIlt
Khi b i t diu thuc hifn cudc cich m^ng xanh, hiu hft cic chuong trinh dfu t i p trung phit trifn cic gidng Ilia cd ning suit cao. Bdi vjy, cic gidng liia die san ciia dia phuong thudng cd nang suit thip d i khdng dupc tm tidn dua vio s i n xuit, thay vio dd l i cic gidng liia lai ngin ngiy, khing siu bfnh, ning suit cao v i khdng Uiom (Bhattacharjee, 2002). Difu nay din dfn su thu hpp vf mjt da d?ng di truyfn, cd nhifu gidng liia chit lupng dia phuong da khdng cdn trong sin xuit, thjm chi hi m i t ngudn gien (Bemer.
1986; Ghareyazie. 1997; Singh. 2000).
Hifn nay. nhu ciu cua thi trudng v i ttii hifu ciia ngudi Ufu dimg d i thay ddi, djc bift nhu ciu vf g^o chit lupng ngiy cing gia ting. Dn dd, vifc ttiu ttljp v i dinh g i i cic ngudn gien phpc vv cdng tic chpn tao cic gidng liia chit lupng dang li vin df dupc r i t nhifu nudc trdn ttif gidi quan tim. Chinh vi vJy, df tii: 'Nghiin ciiu da dang di truyin tip doin Ilia chit luqngbin dia cda Viit Nam bing chi Oil phin ttrSSR vl vi dnh (microsate!hte)"Ai duptc Ufn hinh nhim dinh g i i da dang di Uuyfn, x i c dinh mft sd ngudn
Vifn Di truydn Ndng nghifp Sd Giio dye vi Dio tao Tuyfn Quang
gien Ilia b i n dia quy phvc vv cho cdng tic hio ton, khai thic v i su dpng cd hifu q u i c i c ngudn gien liia chit lupng b i n dia ciia Vift Nam.
L VAT 1 ^ Vll PHUMC PMP N G N A CW
1. Vjt Ufu nghifn Cliu
- Vjt Ufu nghifn ciiu l i tjp doin bao gdm SO gidng Ilia c h i t lupng dupc thu thjp d nhifu dia phuong khic nhau, dang dupc luu giii v i bao tdn lai Ngin hing Gien Ciy trdng Qudc gia (Trung tim Tai nguyfn Thvc vjt) v i Ngin h i n g Gien c i a Vifn Liia ddng bing sdng Cuu Long (bing 3).
- 31 cjp mdi SSR dupc sir dvng df phin tich do Hang Invitrogien cung c i p dva vio cic thdng tin vf trinh tv, kich thudc, sd alen trfn mdi locus, vi tri phin bd cua c i c locus it U*n 12 nhifm sac ttif khSc nhau d i dupc McCouch cdng bd (McCouch et al, 2002) ( b i n g l ) .
2. Phuong p h i p nghifn Cliu
Tich chiit ADN tdag sdi: miu l i cda timg cSy dupc thu thJp rifng rd v i tich chift ADN tdng so theo phuong phip cd sir dpng CTAB ciia Obara va Kako (1998) dupc cii Ufn (Obara v i Kako, 1998).
Thinh phin vi chu tnnh nhiit cua phdn phsn img FCR-Tong ttif tich cua phan ling PCR l i 15 iJ
26 NONG NGHlfP VA PHAT TRIEN iiA»ly-» T U A M k k.1^^ A f A A V
KHOA HOC C O N O NOHj
bao gdm: 1,5 ul Df m PCR lOx, 1,2 |ll MgClj 25 mM, 0,3 pi dNTPs 10 mM. 0,3 pi Taq ADNpolymeraza 5 u/til, 1,5 vl mdi xudi 10 pM, 1,5 pi mdi ngupc 10 pM, 1 pi ADN (30 ng/pD, 7,7 pi nudc cit hai lin k h i ion.
Cic phin ung PCR dupc ttivc hifn theo chv trinh nhift 94<'C (5 phut), 35 - 37 chu ki [94<'C (40s);
SS'C - 60°C (30s), 72»C (30s - 1 phiit)] v i kft ttnic * 72''C(5phit).
Diin di sin phim PCR vi nhudm: Sin phim PCR dupc difn di U-f n gel polyacrylamit 6,0% v i dupc phit hifn dudi tia cvc tim bing phuong phip nhufm ettiidium bromide.
TT 1 2 3 4 5 6 7 8 9 10
u
12 13 14 15 16
Bing Tdn mdi
RM13 RM30 RM282 RM135 RM142 RM143 RM270 RM153 RM162 RM171 RM172 RM174 RM224 RM245 RM257 RM264
l.Tfn,vi NST
5 6 3 3 4 3 12 5 6 10 7 2 11 9 9 8
tri tlia nhifm sic thf v i kich thudtc c i a Kich ttiudc
fbni 124-154 171-183 129-150 119-131 235-238 195-207 104-117 196-234 210-238 310-356 159-165 207-222 120-152 136-146 120-170 148-178
TT 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Tfn mdl RM316 RM284 RM302 RM309 RM286 RM310 RM318 RM323 RM337 RM341 RM515 RM17 RM229 RM166 RM223
c i c cJp mdi SSR NST
9 8 1 12 11 8 2 1 8 2 8 12 11 2 8
Kich ttiudc rhni 186-214 141-149 120-191 158-180 99-128 85-120 134-154 241-244 154-194 126-186 205-231 160-171 108-140 315421 139-163
Phin tich vi joi ly sdMu:K€t qua du^c thong kS dira vao su xuat hi$n hay khong xuat hi^n c^c bang ADN (cac alen). So li6u duoc xii ly, phSn tich bang chirong trinh Excel version 5.0 v^ phdn m^m NTSYSpc 2.1.
He so PIC (Polymorphic Information Content- Lugng thdng tin da hinh) dupc tinh theo cfing thiic sau:
PIC =1 - 2P,2 (trong do P. M tdn s6 xudt hi^n cua alen thli i).
T^ te di hpp (H) cua moi mau dirpc tinh theo cong thiic://% = X
Trong do: X: ia tdng so m6i c6 xudt hi^n 2 alen/1 locus SSR; M: ]k tdng sd moi sir dung trong nghifin cuu; Y: la tong so m6i SSR khong xudt hi^n bang ADN.
Ty 1$ khuyet so li§u (M) dirpc tinh b ^ c6ng Z_
M thiic: M%
M-Y
Trong do: Z \k tdng sd moi kh6ng xudt hi^n bang ADN; M \k tdng sd moi sir dung trong nghifin ciiu.
•.KET QUA VATHAO LUAN
1. H § s d P I C , s d a l e n wk t d n g sd b a n g A D N t h d h i ^ n trfin t u n g c $ p m d i
NONG NGHIEP VA PHAT TRIEN N 6 N G T H 6 N - KY 1 - THANG 9/2012 27
KHO* HQC COMO MOH>
ph lii, ba 3,8 ca gii hi, ml nh ca Da hif Da df Nf
28 TT
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
Tfncfp mdl RM13 RM30 RM282 RM135 RM142 RM143 RM270 RM153 RM162 RM171 RM172 RM174 RM224 RM245 RM257 RM264 RM316
Bing 2. Sd alen ttif VI tri g i n
mAKNST)
5 6 3 3 4 3 12 5 6 10 7 2 11 9 9 8 8
S^alen dif hifn 4 2 5 3 1 7 6 5 2 3 5 2 5 3 5 6 2
h l ^ v <
PIC 0,69 0,44 0,56 0,50 0,00 0,46 0,66 0,49 0,31 0,45 0,61 0,45 0,68 0,55 0,70 0,74 0,50
h« ad PIC c i n 31 cin mdl SSR
TT
18 19 20 21 22 23 24 25 26 27 28 29 30 31
Tfncfp mil RM284 RM302 RM309 RM286 KM310 RM318 RM323 RM337 RM341 RM515 RM17 RM229 RM166 RM223 Tdng
Vlti^gin mdl(NSD
8 1 12 11 8 2 1 8 2 8 12 11 2 8
Thing bmh
S^alen ttl j hifn
2 4 2 5 S 2 1 4 5 8 6 3 3 4 120 337
WC
0,44 0,52 0,2,1 0,72 0,70 0,4? 0,(0 0,4«07?
0,75 QMm om
0,62 lfi It 0,52 l u kdt qua Uiu dupc tir bd tifu bin difn di sin dd v i Ka Bfu. Hf sd PIC (Potymoiphk Mrnmn i r a PCR cua 31 cip mdi SSR vdi tip doin 50 gidng Content) dupc xem l i ttiudc do da hinh cua cic alo 1 chit lupng (bing 2) da ttiu dupc tdng sd 1.519 tiia mdi locus SSR (Smitti et al.. 1997). Gii trj RC ng ADN ttiudc 120 loai alen khic nhau ( b r o g binh ciia 31 locus nghifn ciiu ttiay ddi tit 0 (6 2 cip mo 7 alen/locus). Trong dd, xuit hifn 9 alen hifm ft 6 xuit hifn 1 alen li RM142 v i RM323) dfn 0,74 (oc»
p mdi (alen chi xuit hifn duy nhit ft mft miu mdi xuit hifn 6 alen - RM264). Hf sd PIC tnugbiil ng cd tin sd <0,05). Nhu viy, ty If alen hifm xuit ciia 31 c?p mdi nghifn ciiu l i 0,52, ttii^) hon so w n l i 9/120 (-7,5%), ty If alen hifm trung binh ti-f n mft sd cdng tiinh d i du^c cdng bd Irdn ttif gidi vao 1 locus l i 9/31 (29%). Sd alen hifm xuit hifn Vift Nam (Malik etal., 2010; Raj etal 2006- Khii ifu nhit ft locus RM 270 (xuit hifn 3 alen hifm ft HQu Trung vi cs., 2010).
: gidng Tim cd rpt, Tim xoan vi Ning ttiom Chp 2. Ty If khuyf t sd lifu (M) v i ty If dj hpp (H) o). Tifp tiieo l i locus RM515 (xuit hifn 2 alen ciia c i c gidng liia chit Iupng nghifn a h i m o^6ne lua Tim ti-ing Vmh Phiic vi Ving Ba Ty If d| hpp (H) v i ty If sd lifu khuyft (M) dl.
nh. Cic locus RM17, RM135, RM153 vi RM310 c i c gidng liia chit lupng dupc nghidn ciiu dua trfn J xuit hifn 1 alen hifm hrong ling ft c i c gidng kft q u i phin tich vdi 31 c?p mdi SSR v i dupc tiinh p cil nuong, Tim ti-ing Vinh Phiic, Tim nghf hat biy ft b i n g 3.
B i n g l i y If khuyft sd lifu (M) v i t t If d( hpp ( m ri„ ^ . ^ / ( „ ^ 1.-., .^t limnr nrbif n nn, Ky
hifu
Tl T2 T3 T4 T5
Tfn ddng Tim hic Tiy Bic Tim tiing Vinh Phiic Tim den Ha Ddng Tim thom Hai Duong Tim ttiom Thii Binh
N d N G N M,X 0,00 0,00 3,23 3,23 0,00
GHI|P V
H,X
0,00 3,23 0,00 0,00 0,00A PHAT Kj>
hifu
T27 T28 T29 T30 T31
TRI^"
Tfn ddng Khiu tan pdn Nf p cii nuong Khiu nua nuong Khiu muftng hun Ve nuong
wySMr; T U 6 M - if v M,%
3,23 9,68 3.23 3,23 0,00
1 . TUA
H,*
0,00 14,29
000
0,00000
H/** n '201!
KHOA HQC C 6 N O NOHt
T6 T7 TB T9 TIO T U T12 T13 T14 T15 T16 T17 T18 T19 T20 T21 T22 T23 T24 T25 T26
Tim tidn Hii Duotng Tim diing Hii Duong Tim xoin cd riu Hai Tim xoan Bic Ninh Tim nghf hat dd Tim xoan Vinh Phiic Tim xoan Hii Hiu Tim thom i p be Tim Xuan Dai Tam tidu Tim Xuin Hdng Tim Nghia Hong Tim con Tam Nghia Lge Tim Xuin Bic Tim Hii Giang Tim Nghia Son Tim cd rut Timxoan Tim nghen Tam cao ciy
0,00 0,00 0,00 0,00 0,00 0,00 3,23 0,00 0,00 0,00 0,00 3,23 0,00 3,23 0,00 3,23 6,45 3,23 0,00 0,00 3,23
0,00 3,23 0,00 0,00 0,00 0,00 3,33 3,23 0,00 0,00 6,45 0,00 6,45 3,33 3,23 0,00 0,00 6,67 0,00 3,23 3,33
T32 T33 T34 T35 T36 T37 T38 T39 T40 T41 T42 T43 T44 T45 T46 T47 T48 T49 T50
Chifm pua Blio dang Plf liu chCia Khiu khinh Katifu Khiu lao Tf nuong Mao chft Nf p dng tio Ning thom d$c sdn Ning thom chp dio Liia ning thom OM3536 Ning Nhen Thom Lii Thom man Thom liin miia Vang Ba Danh Huyet rdng 4
Tdng Trung binh
3,23 9,68 3,23 6,45 0,00 3,23 0,00 0,00 3,23 3,23 3,23 0,00 0,00 0,00 0,00 0,00 0,00 3,23 6,45 96,97
1,94 0,00 7,14 0,00 3,45 0,00 0,00 0,00 9,68 0,00 0,00 0,00 0,00 9,68 3,23 0,00 9,68 6,45 6,67 0,00 115,95
2,32 I sd lieu b bang 3 cho thay: Ty 1$ khuyet sd lifiu
,M) cao nhat la 9,68% b 2 giong N^p cdi nuong va ,31ao dang; 3 gidng (Tam Nghia Son, Khau Khinh, iuyet Rong 4) co ty lfi khuydt sd li^u la 6,45%. Co 18
^ndng (Tam den Ha Dong, Tam thom Hai Duong,
"am xoan Hai Hau, Tam Nghia Hong, Tam Nghia ,ac, Tam Hai Giang, Tam cd rut, Tam cao cay, Kh^u an pon, Khau nua nuong, KhSu muong hun, Chiem )ua, Pld lau chiia, Khau lao, N^p 6ng tao, Nang thom tac san, Nang thom cha dao, Vkng Ba Danh) c6
±uyet sd Ufiu cr 1 trong tong sd 31 c3p moi tuong /ng vol ty lfi 3,32%. Cac gidng cdn lgi (27 gidng) iiong b\ khuydt sd li^u. Ty 1^ khuydt sd lifiu trung )inh cua 50 gidng lua kha ihip Ik 1,94; khong c6 :idng n ^ CO ty 1^ khuydt sd lifiu Ion hon 15%. Nhu ky, ca 50 gidng nghifin ciiu d^u c6 y nghia phan tich iidng kfi.
' Ty le dj hpp (H) cao nh^t a gidng liia Ndp c ^ tuong la 14,29%, 3 gidng (Mao ch^t, OM3536 vk Tiom man) c6 ty lfi di hgp tir la 9,68%. Tidp den la
^ac gidng Blao dang (7,14%); T ^ cd rut va Vang Ba )anh (6,67%); Tam Xuan Hong, Tam con va Thom
;jn miia (6,45%); Khau khinh (3,45%); Tam xoan Hai ilau, Tam Nghia L^c va Tam cao cky (3,33%); 6 gidng
(Tim trdng Vinh Phuc, Tdm diing Hai Duong, Tam thom ap be, Tdm Xudn Bdc, Tam nghdn vk Nang Nhen) d^u co ty lfi dj htpp tu la 3,23%. Con lai 30 gidng c6 ty 1§ di hop tu bang 0% (dong hpp a ca 31 locus nghifin cuu).
3. K^t qud phdn tich mdi quan h^ di tniyin cua cdc gidng Ilia ch^t lupng nghifin ciiu
Sd lifiu thu dupc tir tifiu ban di^n di san pham PCR cua 31 cap mdi SSR voi tap dodn 50 gidng Itia chdt lupng nghifin cuu dupc thdng kfi va phdn tich bang phdn m^m NTSYpc 2.1, tir d6 thidt lap dupc bang h§ sd tuong ddng di truydn vd so dd hinh cdy ve mdi quan hfi di truydn giua cdc gidng liia chat lupng (hinh 2).
Qua bang h§ sd tirong ddng va so dd mdi quan h^ di truydn giua cdc gidng nghifin ciiu (hinh 2) cho thiy: Hfi sd tuong ddng di truydn cua 50 gidng liia chit lupng nghifin ciiu dao dong trong khoang tu 0,11 ddn 0,87. Dua vao so dd phdt smh chung, a muc tuong ddng di truydn 40%, 50 gidng liia chat lupng nghifin cuu dupc chia thdnh 6 nhom cdch bifit di truydn.
4dNG NGHllP VA PHAT TRIEN NONG THON - KY 1 - THANG 9/2012 29
KHOA HQC C 6 N 0 N 0 H >
•Nhdm 1: Cd 23 gidng ddu 14 cic gidng liia Tim cd hf sd hrong ddng dl huydn dao ddng tit 0,45 ddn 0,87 v i duc?c chia thinh 4 nhdm phy:
- Nhdm 1.1: Gdm gidng liia T i a ^ m tiic Tiy Bic), T12 a i m xoan Hii Hiu), T18 fTim con), T21 (JimHii Giang),T24 CTinixoan),T23 (Tdmcd rvit), T25 a i m nghfn), T26 (Tim cao ciy), T19 (Tim Nghia U K ) , T20 a i m Xuin Bic). Trong nhdm niy, hf sd hiong ddng dl ttuyfn dao ddng 0,45 (glQa gidng T20 vi T21) dfn 0,82 (giOa gidng T25 vi T29).
- Nhdm 1.2: Gdm gidng liia T9 a i m xoan Bic Ninh), T i l a i m xoan Vinh Phiic), T16 a i m Xuin Hdng), T14 a i m Xuin Dii), T15 a i m Ufu), T22
a i m Nghia Son), T13 a i m thom i p b«). T17 ffa Nghia Hdng). Hf sd tuong ddng dl tinydn dao d^
tir 0,45 (glfta gidng T U v i T17) dfn 0,87 (giiia gA T15viT22),
• Nhdm 1.3: Gdm gidng liia T5 a i m thorn Hi Binh), T8 (Tim xoin cd riu Hii Duong), T7 (Ti dimg Hii Dmmg). Cd hf «d tuong ddng dl tiiiyii 0,62 (giiia gidngTSviTT) ddn 0,82 (giiia gidngTSi T8).
- Nhdm 1.4; Gdm gidng liia T6 a i m tidn H;
Duong) vi TIO a i m nghf htft dd) cd hf sd luon ddng di tiTiydn Ii 0,72.
Hinh 1. Anh difn di sin phim PCR cua c i c gidng Ilia nghifn cOni vdi cJp mdl RMS15 (Af: (kmRuJa" DNA ladder Ultn Low)
Coefficient
Hmh 2: So dd hinh ciy vf mdi quan hf dl truyfn gitia c i c gidng liia chit luong nghidn cihi
N 6 N G N G H I ? P V A P H A T T R ' ilCM M A M / ^ T U A K I _ I^V 1 _ TU A .
KHOA H9C C 6 N 0 NOHi
* Nhdm II: Gdm 10 gidng liia nuong cd h$ sd uong ddng dao ddng tii 0,45 ddn 0,74 vd dupc chia hanh 3 nhom phy:
-Nhom II.l: Gdm 2 gidngT27 (Khdu tan pdn) vd
•^8 (Ndp cai nuong) c6 h§ sd tuong ddng di truydn
^0,61.
: - Nhom II.2: Gdm 7 gidng Id T29 (Khdu nua luong), T36 (Ka tifiu), TSl (Ne nuong), T35 (Khdu chinh), T33 (Bldo dang), T32 (Chifim pua) vd T34 Tid lau chua) cd h^ sd tuoTig ddng di truydn dao
;6ng tir 0,45 ddn 0,74.
- NhdmII.3: Chi c6 gidngT38 CTfi nuong).
* Nh6m III: Gdm 2 gidng Itia tdm Id T2 (Tdm dng Vinh Phiic) vd T4 (Tdm thorn Hdi DuonjO, cd fi sd tuong ddng di truydn Id 0,49.
* Nhom IV: Chi cd duy nhSt gidng liia Tdm T3 rdm den Hd Dong).
* Nhdm V: Gdm 2 gidng lua nuong Id T30 (Khdu luong hun) vd T39 (Mao chfit), cd hd sd tuong ddng i truydn 0,41.
* Nhdm VI: Gdm 12 gidng (2 gidng liia nuong vd 0 gidng Ilia chat lupng b midn Nam) hd so tuong dng dao dpng tir 0,43 ddn 0,81 va dupc chia lam 3 hdm phu;
- Nhom Vl.l: Gdm 3 gidng Id T37 (Khau lao), -'43 (Liia nang thom) vd T44 (OM 3536), c6 hd so
Jong ddng dao dfing tir 0,46 ddn 0,55.
- N h o m VI.2: G d m 8 g i d n g l a T 4 0 ( N e p fing t d o ) , '41 (Ndng t h o m d d c s a n ) , T 4 2 ( N a n g t h o m c h p d d o ) , '45 ( N d n g n h e n ) , T 4 7 ( T h o m m d n ) , T 4 8 ( t h o m liin Siiia), T 4 6 CThom Lai) vd T 5 0 ( H u y d t r d n g 4 ) , c o hfi r6 t u o n g d d n g di t r u y d n d a o d o n g tir 0,43 d d n 0 , 8 1 . I - N h o m VI.3: C h i c o g i d n g T 4 9 ( V a n g B a D a n h )
% IV. XET LUAN VA KEN NGM
? T a p d o a n liia c h a t l u p n g b a n d j a c u a Vi^t N a m r h d d a d a n g v e c a c t h a n h p h a n a l e n . K d t q u a p h a n
^ch 3 1 c a p m d i S S R trfin 50 g i d n g liia c h a t l u p n g t h u
^upc t d n g s d 1.519 b a n g A D N , thufic 120 loai a l e n
^ d c n h a u , t r u n g b m h 3,87 a l e n / l o c u s . Hfi s d P I C
> o d p n g tir 0 d d n 0,74 ( t m n g b i n h 0,52). X u a t h i d n 9
^ e n h i d m 6 6 c a p m d i (cdp m d i R M 2 7 0 x u d t h i ^ n 3 Ien h i d m , cdp m d i R M 5 1 5 x u a t hifin 2 a l e n h i d m , 4 3p m d i R M 1 7 , R M 1 3 5 , R M 1 5 3 v a R M 3 1 0 d d u x u a t ien 1 a l e n h i d m ) .
Cdc g i d n g Ilia c h d t l u p n g c 6 d d t h u d n d i t r u y d n k h d c n h a u , t;^ Id di h p p ciia c d c m d u nghifin ctiu d a o d d n g tir 0% d d n 14,29% ( t r u n g b i n h 2,32%). H d s d t u o n g d d n g d i t r u y d n giQa cdc g i d n g d a o d d n g t r o n g k h o d n g tir 0,11 d d n 0,87. C d c g i d n g l u a T d m (df m i d n Bdc), c d c g i d n g Ida n u o n g (b m i d n niii) vd c d c g i d n g Ilia tfi c h d t l u p n g (b m i d n N a m ) d d u n d m b c d c n h d m rifing. Kdt q u d n d y l d m c a sdr dd p h d n l o ^ , x d c d j n h m a r k e r , n h d n d ^ n g c h i n h x d c m d t s d n g u d n g i e n p h y c vy c d n g tdc b d o t d n vd c h p n , t^o g i d n g Ma c h d t l u p n g ciia Vi$t N a m
TJUL^UTHAMKHAO
1. Bemer D. K., Hoff B. J., 1986. Inheritance of scent in American long grain rice. Crop Science 26. pp. 876-878.
2. Bhattacharjee P., Singhal R. S., Kulkami P.
R, 2002. Basmati rice: a review. International Journal o/Food Science and Technology 37pp. 1-12.
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n.
ANALYZING GENETIC DIVERSnY OF NATIVE QUALTIY VARIETIES IN VIETNAM BY MICROSATELLITE MARKERS
Khuat Huu Trung, Nguyen Thi Ly, Dang Thi Tlianh Ha, Nguyen Mlnh Anh "nian Summary
The results using 31 Simple sequence repeat (SSR) markers for analyzing genetic divereity of Vietnam native quaUty rice varieties have obtained a total number of 1519 DNA bands bek>Dg to 120 difTerence alleles (with a mean of 3.87 alleles per loci). PIC value changed from 0 to 0.74 with a mean of 0.52. lliere are 9 rare alleles in 6 locus (7.5%). The rate of homozygosity of quaUty rice varieties are very different in 31 SSR loci, the heterozygosity changed from 0 to 14.29% (with a mean of 2.32X). GeneUc sunilarity coefficients of 50 varieties wer** ranging from 0.11 to 0.87. Based on the gcneUc rclauonship diagram. 50 varieties of quaUty rice are divided in to 6 major groups: Group I consists of 23 varieties with genetic simiUri^
coefficients were ranging from 0.45 to 0.87; group II consists of 10 varieties with the genetic similanty coefficients were ranging from 0.45 to 0.74; group III consists of 2 varieties with geneUc sinularity coefficients of 0.49; group IV have only one variety; group V consists of 2 varieties with genetic similarity coefficients of 0.41; group VI consists of 12 varieties with genetic similarity coefficients were ranging from 0.43 to 0.81. These results are very useful for classification, identification of geneUc resources, preservation and hybidization of quality rice varieties in Vietnam.
Key words: Simple seqenece repeat (SSl^ marker, genetic diversity, homoiygosity. heteroiygosity, i qualityhce.
Ngudi ph4n bi^n: GS.TSKH. T r ^ Duy Quj^
Ngdy nh^n bii: 28/5/2012 Ngiy th6ng qua ph4n bi^n; 31/8/2012 Ngiy duy^t ding: 6/9/2012
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