KHOA HOC C 6 H G NGHt
DAIVH GIA D A DAIXIG DI TRUY^IM T A P D O A M L L I A CHILI HAI\I CLIA V I ^ T IVAIVI B A I M G C H I T H | P H A I V TLT 5SR
N g u y S n T h j M i n h N g u y d t S K h u ^ t H i m T Y u n g ' , N g u y S n T h j NW, N g u y e n T h i i y D i d p ' , Kidu T h j D u n g ' , Nguyftn T h ( IhkoK N g u y d n T h j T h a n h Thiiy^
T6MTAT
Nghien cuu da dang di tniydn tap doAn lua chju h^n ciia Vidt Nam O^ao gdm 39 gidng liia dja phucmg c6 kha nang chju ban tdt va gidng lua tham khao - IR64 m^n cam vdi khd hgn) b a n g chi thj SSR cho th^y: c^c gidng lua dupe nghien cuu lAl da dang vd cSc t h i n h phan alen. Kdt q u i phan tich 23 locus SSR thu dupe tdng sd 82 loai alen, trung binh 3,57 alen/locus. TI1$ alen hidm xu^t hi^n la 8,7% (2 alen hidm xuflt hi$n d c a c cSp mdi RM3467 vd RM5811). Hd sd PIC dao d^ng tir0,22 - 0,77 (frung binh la 0.56). Ti 1$ dj hpp d c a c gidng liia nghien ciJru rfit khAc nhau, dao d^ng tir 0 ddn 14.29% (trung binh la 3,45%), T a p doAn gidng lua nghifin ciiu c6 miic d$ da dang di truydn rSit cao, h$ sd tuong ddng di truydn giua cAc gidng lua dao ddng tir 0.02 ddn 0.91. Dya vdo k h o i n g each di truydn, 40 gidng liia nghien ciru dupe chia t h i n h 4 nhdm Idn. Kdt ht;rp kdt qua phan nhdm cAch bi$t di truydn va nhOng tlidng tin vd kidu hinh chju ban, 8 gidng Ida didn hinh c6 ngudn gdc khac nhau da dupe chpn dd giai trinh tu, tao lap c o sd dij li^u ngudn gien phuc vy cong tkc chQn, t?o gidng lua chiu han cua Vi^t Nam.
Tirkhda: Chith/SSR, chjuhan. da d^ngditruy4n, lua d/aphuong.
LfiATVAMtE
c a y Ilia {Oryza sativa L.) la m d t t r o n g n h i r n g c a y l u o n g t h u c c d k h a n a n g c h i u b a n k e m va k h a n h a y c a m vdi m d i t r u o n g b e n n g o a i . T i n h c h i u b a n ciia lua p h u t h u d c vao n h i e u y e u td k h a c n h a u : k h a n a n g d a m x u y d n ciia rd, k h a n a n g didu c h i n h a p s u a t t h a m t h a u c u a td b a o la, c a c h d didu k h i d n t h o a t h o i n u d e d k h i k h o n g . N h i j n g n g h i d n ciiu vd s i n h ly va di t r u y e n h p c d a c h i r a r a n g : cd k h o a n g 200 g i e n t h a m gia vao c o c h e k h a n g h a n d c a y t r d n g , n h u n g c a c g i e n n a y k h o n g q u y tu vao m d t g i d n g cd d i n h . Vi vay, k h a n a n g va m i i c d p k h a n g b a n r a t k h a c n h a u p h u t h u d c v a o s u cd m S t va b i e u h i ^ n c u a c a c g i e n k h a c n h a u .
Hidn n a y , h a n h a n la n g u y d n n h a n c a n t r d r a t Idn c h o vide p h a t tridn b d n v i m g n d n n d n g n g h i d p c u a n u d e ta, vi v a y vide n g h i S n ciiu, d a n h g i a c a c n g u d n g i e n h d n q u a n d d n tinh c h i u h a n d c a y lua d d n a n g c a o k h a n S n g c h i u h a n , o n d j n h n S n g s u a t t r d t h a n h v^Ln d d t h d i s u m a n g t i n h c a p b a c h va c 4 n thidt. C h i n h vi vay, Ai tai: "Ddnh gia da dang di truyen tap doan lua chiu han cua Vidt Nam bSng chi thi SSR" n h a m m u c tidu t a o l a p c a s d d u lidu c h o
' Vi^n Di tmyen Nong nghi?p Vi?t Nam
^ Tmdng D^ti hpc Su ph^im Ha Npi ' Bp Nong nghi?p va Phat trien ndng thdn
n g u d n g i e n liia c h j u h a n b a n dia c u a Vidt Nam phuc v u c d n g t a c n g h i d n ciiu, c h p n t a o g i d n g lua chju han.
I . VAT UfU VA PHUONG PHAP NGHCU CUU 1. v a t lidu nghifen ciiu
v a t lidu n g h i d n ciiu la t a p d o a n b a o gdm 39 g i d n g liia cd d a c tinh c h i u h a n d u p e t h u t h a p d nhidu dia p h u o n g k h a c n h a u ; c a c g i d n g n a y d a n g dupe luu giij va b a o ton tai N g a n h a n g Gien Cay trong Qu6c g i a CTrung tarn T a i n g u y d n T h u c v^t) va ngan hang g i e n ciia Vidn Liia d d n g b a n g s o n g C u u Long va g i d n g liia t h a m k h a o - 1R64 m S n c a m vdi kho han ( b a n g 1). 2 3 c$p m d i SSR ( d o H a n g I D T - M ? cung cap) d u p e sir d u n g d d p h a n tich t h u d c cac locus RM d u p e c h p n Ipc, d u a vao c a c t h d n g tin vd trinh tu, kich t h u d c , sd a l e n c h u ^ t r d n m d i l o c u s , vi tri phan bo ciia c a c l o c u s d t r d n 12 n h i d m s a c t h d k h a c nhau da d u p e c d n g b d ( b a n g 2 ) .
2. P h u o n g p h a p n g h i d n c u u
T d c h c h i d t A D N t d n g sd: m a u la c u a timg miu g i d n g d u p e t h u t h a p ridng rd va t a c h chidt ADN tdng sd t h e o p h u o n g p h a p C T A B ciia O b a r a v a Kako c6 cai tidn ( O b a r a v a K a k o , 1998).
C h a y PCR: c a c p h a n l i n g c h u d i t r i i n g h p p (PCK d u p e tiiue h i d n t h e o c h u tiinh nhidt: 94°C (5 phut), 35-37 c h u k y [94'*C (40 g i a y ) ; 5 5 ^ 0 « C (30 giay), 7 2 ^ (30 g i a y - 1 p h u t ) ] v a k d t tiiiie d 72''C (5 phiit).
P h a n tich v a x u ly sd lieu: k d t q u a d u p e thdng ke d u a v a o s u x u a t h i d n h a y k h d n g x u ^ t h i d n cua ck b a n g A D N ( c a c a l e n ) . Sd lidu d u p e x i i ly, p h a n tich
NONG NGHlf P VA PHAT TRIEN N 6 N G T H 6 N • KY 1 - THANG 1/2013
KHOA Hpc C 6 N 0 N C H C
bkg chuong trinh Excel version 5.0 v4 phin m^m m6i c6 xuait hi«n 2 aIen/1 locus SSR; M 14 tong s^
NTSYSpc 2.1 (Rohlf, 1997). m6i sit dung trong nghiSn ciiu; Y14 tdng sd mdi SSR H6 sd PIC (Polymorphic Information Content - khdng xuSthi^n bang ADN.
luong thdng tin da hinh) dupe tinh theo cdng thiic l y \i khuyft sd lieu (M) dupe tinh bang cdng sau:PIC.l-ZP,Mtrongd6F, litSnsdxuSthifnciia , . . „ , Z . , , , , , v , ., alen thii i) ""^^ ^'^ = — • ''^""S dd: Z 14 tong sd mdi khdng
Ty 14 di hop (H) cua mdi mau ducjc tinh theo xu4t hifn bang ADN; M14 tdng sd mdi sii dung trong
•^ ^ ^ nghidn ciiu.
cdng thiic: HVo =
-; trong dd: X14 tdng sd M-Y'Bang 1. Danh s4ch, tfen, ki hi^u v4 sd dang ki cua c4c gidng liia nghidn ciiu
TT 1
2 3 4 5 6 7 8 9 LO 11 12 13 A .5 .6 .7 .8 9
;o
SDK H2
ISO .832 .837
;021
!125
!127
!131
!135
!642 1351 1371
•099 1429
!483 1525 i430 (588 .666 1895
Ten gidng Nep bd hong Hai Dirong Liatdn Khau nudt cung Lua md trdng Khaumeo B'le tolo Ble blu Ble la tong Ble lenh xi Ble' la Liia can dd Ndpmam Khau cam pi Chidm dd Liia mud'i BachoK'td Khau ken Tan ngan IR64 Ble ma miia
Kyhidu HI
H2 H3 H4 H5 H6 H7 H8 H9 HIO H l l H12 H13 H14 H15 H16 H17 H18 H19 H20
Ngudn gdc Hal Duong
Qu4ng Nam Quang Nam
Quang Tri Quang Ngai
Binh Dinh
Y6nB4i IREI Son La
TT 21
22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
SDK 3935
3947 3970 4123 4723 4726 4748 4762 4792 4793 4794 4806 4840 4843 5057 5015 5018 5020
Tdn gidng
*ldng lu
Chau bd kh4 Jlech cau Ch^u 15y khao
"ham sodng
•Idp cai can langngua j?cang Chau ma gi4ng Qidu ndn Qiauhin U4o sinh sai J14o ddng il4o cd nem QiSucu [^hao luu Chau san Qiau dd ddn
•^Jang qudt bidn Vangqudtv4ng
Kyhidu H21
H22 H23 H24 H25 H26 H27 H28 H29 H30 H31 H32 H33 H34 H35 H36 H37 H38 H39 H40
Ngudn gdc
LaiCh4u
Ghi chu: SDK sddSngkiangan hang gien; (-) chuaro ngudn gdc; ('): Gidng lua miia dia phuong mien Nam.
•.KcmuiviliiMoiiwii
1. K^t qu4 phan tich da hinh ADN bSng c4c chi
I IfaiphantuSSR
Kdt qua phan tich 23 cSp mdi SSR vdi t?p do4n 40 gidng Ilia nghidn aiu thu ducjc tdng sd 82 loai alen
khac nhau, sd alen/locus dao ddng tu 2 - 5, trung binh 3,57 alen/locus. Ca 23 cSp mdi ddu cho c4c locus da hinh, trong dd cd 3 c4p mdi thu dupe 2 alen, 10 cap mdi thu dupe 3 alen, 4 cap mdi thu dupe 4 alen v4 6 cap mdi thu dupe 5alen(bang2).
I NONG NGHlfP VA PHAT TRI^N N 6 N G T H 6 N - KY 1 - THANG 1/2013
13
KHOA Hpc C O H G NGHt
B4ng 2. Sd alen thd hi{n v4 h^ sd PIC cfia 23 cap mdi SSR STT
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Tdn mdi RM145 RM152 RM267 RM566 RM1155 RM1364 RM1367 RM3288 RM3431 RM3467 RM3468 RM3476 RM3483 RM3515 RM3534 RM5599 RM5811 RM6051 RM7003 RM7372 KM7581 RM25271 RM25319
Vi tri U-dn NST
2 8 5 9 4 7 2 4 6 3 1 5 12 2 4 11 1 9 12
9 1 10 10
Kich thudc s4n phSm PCR (bp)
194-281 151-175 150-180 234-290 120-190 120-194 90-150 13ai90 130-194 95180 180-234 118-190 150-234 110-156 110-150 120-156 90-130 118-156 100-130 100-140 120-220 156-210 120-200 Tdng
Tnmgbinh
Sd alen thd hidn
5 3 3 4 5 4 5 3 2 5 5 4 5 3 3 3 3 3 2 3 3 2 4 82 3,57
Sd alen hidm
0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0,087
PIC 0,61 0,51 0,59 0,72 0,62 0,63 0,77 0,50 0,29 0,70 0,72 0,70 0,72 0,66 0,53 0,53 0,50 0,46 0,46 0,46 0,22 0,38 0,61 12,89 0,56 Ghi chu: NST: nhiSm sic thS; PLC: Polymorphic Information Content
Hd sd PIC dupe coi la thudc do tinh da hinh cua cac alen d timg locus SSR. Qua kdt qua thu dupe d bang 2 cho thay ring, gia tri PIC cua 23 chi thj phan tii thay ddi tir 0,22 (d cap mdi RM7581) ddn gia hi PIC cao nhat la 0,77 (d cap mdi RM1367). Hd so PIC
Bang 3. MQt sd kdt qua phan tich da dzuig di truydn su dyng clu thj SSR trdn cAy liia da dugc cdng bd trung binh cua 23 c^p mdi nghidn ciiu la 0,56. Co 2 alen hidm xuat hidn (alen cd tan sd xuat hidn nho hon 5%) d cac c^p mdi RM3467 va RM5811 (chidm li Id 8,7%). Nhiing kdt qua thu dupe cung tuong dirong vdi mdt sd kdt q u i nghidn ciiu trdn thd gidi (b^ng 3).
TT 1 2 3 4 5 6 7 8
Tac gi4 NagarajuJ.,2002 Yu S. B., 2003 Chakravarthi B. K, 2006 Giarrocco L E., 2007 Alvarez A , 2007 HerreraT.H.,2008 WongS. C.,2009 Nghidn ciiu n4y
Sd gidng
24 193 15 69 50 18 8 40
Sd chi thi
19 101 30 26 10 48 12 23
Tdng sd alen
70 628 462 219 66 203
31 82
Trung binh Sd alen thd hi^n/locus
3,8 6,2 8,4 6,6 4,23
2,6 3,57
PIC
0,68 0,69 0,74 0,52 0,52 0,56
Sdalen hidm/locus
1,7 1,4
0,087
N 6 N G NGHIEP VA PHAT TRIEN N 6 N G T H 6 N - KY 1 - THANG 1/2013
KHOA H9C C 6 H G H G H |
l l S h p l 7 2 b p l
Hinh 1. Anh didn di sin p h i n PCR ciia 40 gidng liia nghidn ciiu vdi c ^ mdi RM3468 (M: aX17- Hea III digest DNA ladder)
2. Ty Id di hpp (H56) v4 ty If khuydt sd Udu (M%) ciia 40 gidng liia nghidn ciiu
Bing 4.Ty Id dj hpp (H%) v4 ty If khuydt sd lif u (M%) ciia 40 gidng liia nghidn ciiu TT
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Tdn gidng Ndp bd hdng Hai Duong Uaton
Khau nupt cung Lua mp trang Khau meo B'le tolo Ble blu Ble la tong Ble lenh xi Ble' la Lua can do Nep mam Khau cam pi Chiem dd Liia mudi B a c h o K t d Khau ken Tan ngan IR64 Ble ma miia
M%
4.35 0.00 0,00 4,35 4,35 0,00 0,00 0,00 0,00 4,35 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00
H%
0,00 0,00 4,35 0,00 0,00 4,35 0,00 0,00 0,00 0,00 4,35 0,00 0,00 4,35 8,70 8,70 8,70 4,35 0,00 0,00
TT 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
Tdn gidng Mdng lu Khau bd kha Blech cau Khiu ISy khao Cham sodng Ndp cai can Hang ngua Locang Kh^u m4 giang Khau ndn Khau hin Blao sinh sai Blao ddng Blao cd nem Khiucu Chaoluu Khau san Khau dd ddn Nang qudt bidn N4ngqu6tv4ng Trung binh
M%
4,35 0,00 4,35 8,70 0,00 0,00 0,00 4,35 0,00 0,00 0,00 0,00 8,70 0,00 0,00 0,00 4,35 0,00 13,04 8,70 1,85
H%
4,55 0,00 9,09 4,76 4,35 4,35 0,00 4,55 4,35 4,35 0,00 8,70 14,29
0,00 4,35 8,70 4,55 4,35 5,00 0,00 3,45 Qua kdt qua thu dupe a bang 4 cho thay, ty 14 dj
hpp a cac gidng liia nghidn ciiu rat kh4c nhau. Ty Id di hop (H«) cao nhat a gidng liia B14o ddng 14 14,29«, tidp theo la gidng liia Blech cau cd ty Id dj hpp 14 9,09*. C4c gidng liia Liia mudi, Ba c h o Ktd, Khiu ken, B14o sinh sii v4 Chao lim ddu cd ly Id di hpp 14 8,70%. Gidng Iiia N4ng qudt bidn cd ty Id dj hpp 14 5,OOS6. Gidng liia Khau 14y khao cd ty Id di hpp la 4,7696. Ba gidng liia Mdng lu, Lp cang v4 Khiu s4n cd ty 16 di hpp 14 4,55«. C4c gidng liia Khiu nupt cung, Ble tolo, Liia can do, Chidm do, Tan ngin, Cham sodng, Ndp cii c?n, Khiu m4 gi4ng, Khiu ndn, Khiu cij va Khiu dd ddn ddu cd ty If di hpp 14 4,35%. 17
gidng cdn lai cd ty Id di hop 0% cd nghia 14 c4c gidng n4y ddu ddng hpp d ca 23 mdi nghidn ciiu (chi cd 1 alen duy nhit/locus). Ti Id dj hpp trung binh cua c4 tap do4n la 3,45%.
Ty Id sd hdu khuydt (M%) cao nhit d gidng liia N4ng qudt bidn 14 14,29% (khuydt sd lidu a 3 mdi).
Cac gidng Khau lay khao, B14o ddng v4 N4ng qudt vang cd ti Id khuydt sd lidu la 8,7% (khuydt sd Udu d 2 mdi). Cic gidng Ndp bd hdng Hai Duong, Liia md trang, Khiu mdo, Ble' la, Mdng lu, Blech cau. Lp cang, Khau san cd ti Id khuydt sd lidu 14 4,35%
(khuydt sd lidu a 1 mdi). Cdn lai 28 gidng khdng bi khuydt sd hdu d" t i t ca cic mdi. Ty Id khuydt sd Udu
N 6 N G NGHIfP VA PHAT T R I I N N 6 N G T H 6 N - K9' 1 - THANG 1/2013
15
KHOA H9C C 6 N G WOHt
trung binh cua ca tip do4n 14 1,85%; khdng cd gidng n4o cd d If khuydt sd lifu Idn hon 15%. Nhu vSy, c i 40 gidng nghidn ciiu ddu cd y nghia tlldng kd trong phan tich da dang di truydn.
3. Kdt q u i phin tich mdi quan hf di truydn ciia cic gidng Ilia nghidn cihi
Sd lifu thu dupe til kdt q u i difn di sin phJm PCR cua 23 cjp mdi SSR vdi tip doin 40 gidng liii nghidn ciiu dirpc thdng kd v i phin tich bang phfe mdm NTSYSpc 2.1, tii dd thidt Up dupe bing hf si tuong ddng di truydn v4 so dd hinh ciy vd mdi quan hf di truydn giiia cic gidng liia chiu han (hinh 2).
—
H
1
1 ^ = —
H 1
( — ^ —
L
H <
1 1
- ^
CoefTicient
Hinh 2. Sa dd hinh cay vd mdi quan hd di truydn giOa cic gidng liia clyu h ^ nghidn cuu Bang he sd tuong ddng va so dd vd mdi quan hd
di truydn giiia cac gidng nghidn ciiu (hinh 2) cho thay: hd sd tuong ddng di truydn ciia 40 gidng liia chiu han nghidn cuu dao ddng trong khoang tu 0,02 ddn 0,91. O muc tuong ddng di truydn 26%, 40 gidng Ilia chiu han nghien ciiu dupe chia thanh 4 nhdm each bidtdi truydn.
* Nhdm I gdm 3 gidng lua: Ndp bd hdng Hai Duong, Nang qudt bien va Nang qudt vang. Hd sd tuong dong di truydn giua cac giong trong nhdm dao ddng trong khoang 0,26 (giira gidng Ndp bd hdng Hai Duong Va Nang qudt bidn; gidng Ndp bd hdng Hai Duong va Nang qudt vang) ddn 0,91 (giua gidng Nang qudt bidn va Nang qudt vang).
* Nhdm n gdm 11 gidng dupe chia thanh 2 phan nhdm:
PhSn nhom 2.1 gdm 2 gidng: Liia mp trdng va Khau meo cd hd sd tuong dong di truydn la 0,42.
Phan nhom 2.2gom 9 gidng dupe chia thanh 2 phan nhdm phu:
+ Phan nhom phu 2.2.2 gom 6 gidng: Ble lenh xi, Chidm dd, Liia mudi, Lua can dd, Ndp m§m va Ble' la, cd hd so tuong ddng di truydn dao dOng tir 0,42 (giiia gidng Ble' la va Liia mudi) ddn 0,74 (giiia gidng Ble lenh xi va Chidm dd).
+ Ph^ nhdm phil 2.2.1 gom 3 gidng: IR64, Blech e^u va KhSu lay khao, cd hd sd tuong ddng di tniy&i dao dpng hi 0,53 (giua gidng Blech cau va Khau lay khao) ddn 0,62 (giiia gidng IR64 va Blech cau).
* Nhdm III gdm 6 gidng: Lia tdn, Khau nuot cung, B'le tolo, Ble blu, Ble la tong va Khau cam pi.
Hd sd tuong ddng di tinydn giua cac gidng trong nhdm dao dOng trong khoang 0,28 (giua gidng lia tdn va Kh^u nupt cung) ddn 0,64 (giua gidng Ble blu va Ble la tong).
* Nhdm IV gdm 20 gidng dupe chia thanh 3 phan nhdm:
+ Ph^ nhdm 4.1 gom 8 gidng Ba cha Ktd, Khiu ken, Cham sodng, Lp cang. Tan ngan, Ble ma miia, Kh^u bd kha va Mdng lu cd hd sd hiong ddng dao 16 NONG NGHI?P VA PHAT TRIEN NONG THON - KY 1 • THANG 1/2013
KHOA H p c C 6 N G N G H I
ddng tir 0,37 (giua gidng Ba c h o K'td va Mdng lu) ddn 0,70 (giiia gidng Ble ma miia va Khau bd kha).
+ PhSn nhom 4.2 gdm 7 gidng: Ndp cai cgn.
Hang ngua, Khau ma giang, Khau ndn, Biao sinh sai, Blao ddng va Kh^u hin. Hg sd tuong ddng di truydn giua cac gidng trong phan nhdm nay dao dpng tir 0,38 (giiia gidng Hang ngua va Kh^u ndn) ddn 0,85 (giiia gidng Kh^u ma giang va Khau ndn).
+ Phan nhom 4.3 gdm 5 gidng Blao cd nem, Khau cu, Chao luu, Khau san va Khc'iu dd ddn, cd hd sd tuong ddng dao ddng tir 0.47 (giua gidng Blao cd nem va Khau dd ddn) den 0,75 (giua gidng Khdu cu va Chao luu).
•V.KFTLUAN
Tap doan gidng liia chiu han ban dja ciia Vidt Nam kha da dang vd cac thanh phan alen. Ket qua phan tich 23 chi thi phan tii SSR vdi 39 gidng liia chiu han va gidng IR64 thu duoc tdng sd 82 loai alen, trung binh 3,57 alen/locus. He sd PIC dao ddng tir 0,22 ddn 0,77 (trung binh 0,56). Ti Id alen hidm xuat hidn la 8,7% (2 alen hidm xuat hien d cac cap mdi RM3467vaRM5811).
cac gidng Ilia chiu han cd dd thuan di truyen khac nhau, ty Id di hpp cua cac gidng liia nghien ciiu dao ddng tir 0 ddn 14,29% (trung binh la 3,45%). Miic 1 dp da dang di truydn giira cac gidng liia chiu ban rat . cao. He sd tuong ddng di truydn giiia cac gidng liia . dao ddng tir 0,02 den 0,91. Dua vao khoang each di truyen, 40 gidng nghien ciiu dupe phan thanh 4 nhdm each biet di truyen. Qua dd, da chon dupe 8 ' gidng Ilia dien hinh cd dp da dang cao va cd ngudn
gdc khac nhau de giai trinh tu, tao lap co sd diir lieu
* ngudn gien phuc vu cdng tac chpn, tao gidng liia
"f chiu ban ciia Viet Nam.
' Ldi cam on: Nghien cuu nay duoc thuc hidn
^ trong khudn kho de tai Nghi dinh thu hop tac voi '* Vwmg quoc Anh:"Nghien cuu giai mS h$ gien V (genom) mot sogiSng liia dia phuong cua Vidt Nam ".
TAI U a i THAM KHAO
1. AlvarezA., FuentesJ. L., PulddnV., GdmezP.
^., Mora L , Duque M. C , Gallego G. and Tohme J. M.
'(2007). Genetic diversity analysis of Cuban traditional rice {Oryza sativa L.) varieties based on -microsatellite markers. Genetics and Molecular fBioiogy,zo {A): iim-nn.
N 6 N G NGHIEP VA PHAT TRIEN NONG T H 6 N - K\' 1 - THANG 1/2013
2. ChakravarthiB.K., and NaravaneniR. (2006).
SSR marker based DNA fingerprinting and diversity study in rice {Oryza sativa. L). Ahican Journal of Biotechnology, 5 (9): 684-688.
3. Giarrocco L. E., Marassi M. A. and Salerno G.
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organization of the parental lines us<*d in the Theor. Appl Genet, 108:131-140.
International Rice Molecular Breeding Program.
ANALYZING GENETIC DIVERSITY OF NATIVE DROUGHT TOLERANT RICE VARIETIES IN VIETNAM BY MICROSATELLITE MARKERS
Nguyen Thi Minh Nguyet', Khuat Huu Trung', Nguyfin Thi Nhai', Nguyen Thuy Diep', Kieu Thi Dung', Nguyen Thi Thao^, Nguyen Thi Thanh TTiuy'
Summary
AiinKviiiR Ri'iuMir diversity of native drought tolerant n r r varieties in Vietnam (including 39 local nce varieties tolerant to drought and lR64-reference vanrty which Is sensitive to droughO by SSR markers showed that: Tlirsc drought tolerant ncc vaneUcs have diversity about allele components. The results using 23 SSR markers for analyzing genetic diversity of 40 varieties have obtained 82 differenrcs alleles, with an aver.iKc of 3.57 alleles per SSR loci. Tlie ratio of rare alld.- is 8.7% (2 rare alleles occur at RM3467 and RM5811). PIC value changed from 0.22 to 0.77 (with a mean of 0.56) which showed high diversity of the studied SSR loci. The ratio of liomozygosily of naUve drought tolerant rice varietjo;. are very different m 23 SSR loci. The heterozygosity ohanRed finiii 0-14.29% (with an average of 3.45%). The ginetic similarity coefficients of 40 varieties were ranging from 0.02 to 0.91. Genetic similarity was determined using Jaccard's similanty coefficients and final tiedrograni construction using a UPGMA clustering methods showed that 40 varieties were divided into four major groups, which shows great diversity among vaneties.
Based on genetic clustenng result, drought tolerant phenotype and the origins of rice varieties, eight varieties have been selected as materials for further research on establishment of a database for local rice genetic resources in Vietnam.
Keywords: Drought tolerance, genetic diversity, local nce vaneties, simple sequence repeat marker.
Ngudi phan bidn: GS.TSKH. Trdn Duy Quy Ngay nhan bai: 2/11/2012
Ngay thdng qua phin bidn: 28/12/2012 Ngay duydt dang: 07/1/2013
' Agricultural Genetics Institute ' Hanoi University of Education
^Ministry of Agriculture and Rural Development
