I Kt}oa hoc Ky thutt va Cdng nghi
So sanh qua trinh thu nhan cao chiet rong nau ^ai /«^
bang cac phirong phap chiet Ivhac nhau va danh gia Kna khang oxy hoa b k g phu-ong phap DPPH
nia* cti ivt-n t*'-^ ^^'^
TrinlhiNuix•^lai• „„rni p;ilH.„S,ndiapiiM'«"SlOI"»-"
- i l : ;\ nh in ph.m H\
^™ ""• .. „, c „ „ a , j , , m polynstiim t h u tCr viing bien NchiOii cihi d u v c lien h i n h Iren hai laai rong nau Sarga^^umfeldmaimii'.i '. | , j p . Soxhlet, sir d u n g song kh:,ril, lloa >i M n h T h u a n . Thu nhan cao chiet tir hai loai rong naj hiing oJ P ? >^ ^ j j j „ j „ cho li le t h u sieu i n , , a c h i i . ke, h.^, >u Is e „ „ n , e \ ise.usnu. 1 KO, ,u^a. P h - ' ' " ' ' P / ' ' J ^ ^ ^ ^ ^ M , g ^ ^ ^ D P P H t u m i g
u n s la ( , . . . l l t - „ s i 6 : . - : , l " . . 1),-., sdi p h u o n g phap s u Is M s e o z , n , e L " " ' ' ' « ' " " l ung la 6 1 , 6 4 2 % v4 6 2 , 7 6 6 % . .V./eM™„„,7 , i 8.020.0.186% cS. p«ly.y^,u,n: kha n i n g b i t goc ' M " D W H r S l e t , t, le t h u h6i c a o c h i l t Hal p h m m g p h i p n i s cho hlv-u qua cua q u i Irinl. chie, cao hon so v6l phuomg phap M x n ,
l i 6 , 7 7 4 , 0 . 0 6 0 - : o 5 : / e « J ™ / ! ' s i 6,.S75.«,4I.VV i, S. p.lycy.,um kha niing hat goc tu do D P P H t u a n g u n g 54..H)ro v;i 5(..?ll.^"„ Ml vffiehai doi chieu la vitamin C ff ciing nong dg.
Tir khda: phinmR phap DPPH. Sargassum. .Sovhiet, song s thi^dohdnlimnlA
Bll vin aft
Kmi}; bien lu nguon tai nguyen phong phu. da dang ve ehiiiiL: lo;ii Theo ihong kc, nirae la c6 khoang 794 loai rong hien. phan Ki a viing h e n phia B3c 310 loai. phia Nam 4S4 loai. 1.^6 Icii tim ihJv a CJ hai mien. San lu-cmg cua ehiing luoTig doi iiin vai mat do irung binh 43,8^2(1.2 cay m- vii sinh l i r ^ g tmng binh dat 45(i. 1 -64,2 g kho/m'. Rong bien. dJe bift la rung nau c6 chira cac thanh phan co hoat linh sinh hpc cao nhir fucoxanihin, a-locopherol, fucosterol.
ji-ciroicii, cae polyphenol... [1-.^]. Trong rong nau chira lony lu^mg v.i-y can trung binh khoang 20%. ci)-9 chiem khi>.ing 15% va (o-6 (arachidonic acid) chiem khoang 9 % 14, >] Do do. \ iec \ir ly rong de thu chat chiet la can thiel.
SKirng nghien cwti chiet tach cac hgp chat co hoat linh sinh hpc hucmg den vi^c bo sung chiing \ a o ihyc pham nham lAng gia trj chiic n^ng cua thtrc pham da v i dang nhan dugc s\r quan tam ciia cae nha khoa hoc Irong nunc va tren ihe giiiti jfi-S]. Trong nghien ciru nav. cac phuiTng phap chiet duge su dung la cac phuong phap Soxhiei. su dung song sieu am, ngam chiet ket hi.7p \ u ly enzyme
PhuiTiig phap '»u dung song sieu iim la phuang phip mm dugc ung dung trong kv ihual phan tach. chiel vuat hicii UJV VI.T1 ini diem ch.> hieu 'iuai ihu nhan eao hem --i' "sOi phL^iini;
phap ihong ihiii'ii. iho ^MH UKII IV ng.in, ch.ii lumig cu- san pham tin Lh, n ihien . cn moi innmg v;i nhieu tiem nang
J am, \'i,scozynie L.
ling dung a quy mo cong nghiep. Sieu a m la song c o hgc hinh Ihanh do sir lan truyen dao dpng ciia cac phan tir trong khong gian co (m s6 khoang 20-100 kHz, Song sieu am c6 dp dai song khoang 1 0 - 1 0 ' cm nen khong du nang l u g n g de tuong tac iruc ti^p len lien ket hoa hpc, Tuy nhien, s y phat song sieu am trong moi truong long lai san sinh ra mpt nang lugng lon, gay nen hien tugng val ly ggi la s y tao va v a bgt khi. Khi lruy6n song sieu am qua moi truong long, d u a i tac dung ciia song, cac bpt khi bj keo nen, sy tang ap suat va nhiet do lam cac bgt khi no vo. K.hi s y no v a ciia cac bgi khi 6 gan be mat pha ran, xay ra s y mat doi xung, sinh ra tia dung moi co toe do cao vao thanh le bao, do do lam tang sy xam nhap ciia dung moi vao te bao va lam tang be mat tiep
\ u c giua pha ran va pha long Dieu nay lam tang s u Iruyen khoi va pha v a cau triic te bao (hinh 1). Su no v o cua cac bot khi lam lang sy thoat ra ciia cac chat npi bao vao dung djch [9].
Enzyme Viscozymc L chira cac enzyme bao g6m arabanase, cellulase, (l-g!ucanase. hcmicellulase xvlanase va pentosanase; thuy phan ngau nhien lien kSt R-1 4 - gl>cusidccuacacglucan(chuy6ulacelluh>...Ko,i,)ngthanh le bao rong de giai phong ra celluloscdextrm. ccltobiose va j; ucose (hinh 2). Viec s u d u n g enzyme Vis^Mcnzvnit: I u"' ... tac phan huy thanh le bao rone ho irii i-rr tfm.i k,- -
C
NGHE > -• \ani HOC . \ ,Khoa hpc Ky thu$t va Cong ngh^ i
Comparison of extraction process from brown seaweed Sargassum by different methods
and evaluation of antioxidant possibility by DPPH method
Thi Ngoc Mai Tran' Institute of Applied Sciences, University' ofTeclmology-,
Ho Chi Minh city (HUTECH) Received 22 November 2019, accepled !0 January 2020 Abstract:
The study was conducted on two types of brown seaweed Sargassum feldmannii and Sargassum polycystum which were collected from the sea of Khanh Hoa and Ninh Tliuan provinces. Acquisition of extraction from the samples was operated by three methods:
Soxhlet, ultrasound method and method using enzyme Viscozyme L. Results of ultrasound method showed that the extract recovery was 7.095±0.055% in S.
feldmannii and 8.373±0.122% In S. polycystum; their free radical scavenging activities (DPPH) were 63.037%
and 62.720% respectively. For methods using enzymes Viscozyme L, the extract recovery was 7.192±0.213% in S. feldmannii and 8.020±0.186% in S. polycystum; their free radical scavenging activities (DPPH) were 61.642%
and 62.766% respectively. The effective extraction of these two methods was higher than that of the Soxhlet method which the extract recovery was 6.774±0.060% in S. feldmannii and 6.575±0.413% in S. polycystum; their free radical scavenging activities (DPPH) were 54.301%
and 56.503% respectively, compared to the reference substances as vitamin C at the same concentration.
Keywords: DPPH method, Sargassum, Soxhlet method, ultrasound, Viscozyme L.
Classification number: IA
S6nfl BUU am (US)
.Q.
Hinh 1 Qua trinh tac dong ciia song sieu am len te bao (9).
xylofLFCo.glucan TP w M Kyloluco-glycuFDnan
• " fucan llAng I d i
^ ri£n kAl qlycoprolote
Hinh 2, Cau tao thanh te bao rong nau [5].
Vdi Ii0u va phiTdng p Vdt lieu
Nguyen lieu rong mo thu tir viJng bien K-hanh Hoa co ten khoa hpc la Saigassum feldmannii va rong chi Sargassum polycyslum thu lir viing bien Ninh Thuan, Rong dugc ngam, nja sach, phoi/say a 45-50"C den kho (dp am 16%) va xay nghien nho thanh bpt voi 3 kich ca 0,25; 0,5 va 0,63 ram.
Viscozyme L duoc san xuat tir nam Aspergillus acidealiis.
hoat dpng tor a pH 3,3-5,5 va nhiet dp 25-55°C, Viscozyme L ban chat la p-g!ucanase {endo-1,3(4)-) chira cac enzyme bao gom arabanase, cellulase, P-glucanase, hcmicellulase, xylanase va pentosanase. Hoat tinh 100 FBG/g.
Phirffng phdp nghien ciru
Phircmg phdp Soxhiei: can M, (g) bpt rong nau da dugc say kho den khoi lugng khong doi cho vao bp chiet Soxhlet, tien iianh chiet. Sau khi ket thiic qua trinh chiet, bpt rong dupc say den khoi lirpng khong doi va can dugc M^(g).
Phircmg phdp sir dung song sieu dm: thiel bj phat song sieu am sir dung trong nghien ciiu la thiet bi sieu am dang thanh.
Mau dugc chuan bj trong cac coc, tihiing thanh phat song sieu am vao dung dich long - ran (dung moi - nguyen lieu). Cac thi nghiem khao sat thyc hien a ciing lan so 20 kHz. Sau khi trich ly M (g) bpt rong nau bang song sieu am, tach ba bang may ly tam voi toe dp 3500 vong/phul trong 10 phut, toan bp ba dugc say den khoi lugng khong doi dugc M^{gJ. Hon hpp dung moi va chat chiet duac dua qua he thong co quay chan khong de cat loai dung moi. san pham thu dugc a dang cao chiet kho kiet.
Phuong phap chiel kel hgp xir ly enzyme: bpt rong dugc
35
I Khoa hpc Ky Ityuit vi Cdng ngtti
ngJm iront: nuvK v iii n ic 1 ^. sau d- ^-Jl' -^at q - - :nnh bo >ui _ fii/vnie \'isco/\mc I. ^au t^hj,- sal ba KM rong dui'c ihu hi ^ cieh Iv lim '5IMI song phul. UIT.J 10 phul vj sav c 4?-M'v Jendo.inib.in Jju l^'' * ,i'i M (C'fv't rong •^u \ c Iv cimn-e.
ticn hanh ehiel bSngphuimg phap ngam lie IfMivone phui trcr, mav lie. su dung dung moi dichioromeihane mcin.iiol la . ll le dung moi neuvt-n hpu la "" 1, thi^i gian chiet la '^ -,''• ^ J - khi ki-l thuc qua irinh chiet. ba bgi rong Ju.'^ ihu hing cach ly tam 15(Hl vong phul, trong H) phul. -^y Jen Jo am ban dau v.i can dupc \1 ii;i Hon h(.Tp dung moi .aehalchieuluoc Ju-iqu-i he liiong eo quav ch.in khong de ^.il livai dung nioi. ^.in phJm thu dugc o dang cao ehiel kho kiel
Tl le thu hi'i L.IO chii-l co irong lIKi g r.guvi-n lieu linh ihco eong thifc NJU
Tl K- Ihu hill cao chii-t i M ^ ^ ^ lOO ( "
I'hmmg I'luip ni'PII l.|.dinhcn\l-:-picrylh>dn/yi (DPI'in la mpl giv ly do bi'n, co nuu lim ui co do h.ip ihu a l l dai obuo*. •-one f'\'^ nm khi eo m.')! ihai chimg ovv ho.i, no scMi| khu llunh : :-diphenvl-l-picrylhvdr.i/irie iHriMI-H), CO iiiiiu V ane Oo do h.ip ihu lai buoc M>nu .'' 1" nm de v.!C dinh kh,i nSng khu giK DPPH cua ch.ii chong o\> hoa trong mau can phan tich .\cid ascorbic du^'L ^u dung lam chal doi chieu.
Phiin trim bit gi>s. ty do DPPH cua mau cm plian tich dugc linh iheo cimg thirc
11 K- k-ii giK ty do DPPH ( M - '-^-^ ^ ' " " (-i uong do: o n l.i mat JO quang cua ihung (dung moil; OD, la ni,il di^> qu.iiie cua mau thir hav doi ehieu
Plmmgpiiap u / / i -••' lieu lai CJ M'I heu dugc bieu dien diroi diing Tmng hmh-sl) o muc v nghia i'" (1.05 Su dung phan nicm \ u Iv vo livu Siaigraphics Centurion XV l i t qua vi bin iBftD
C hii-f hdtig pbirtmg phdp So.vhiet
Kieh ihmic nguven lieu \a vice lya chon dung moi se anh hucmg den li li- cao ehiet thu hoi Viec xay nho nguyen lieu gop phan ph.i \iT \ach li- bao de dang hon. tang dien iich tiep xuc giua nguven li\'u va dung moi. tang kha nang khuech tan ciia cac ch.il lan vao clung nioi. tao dieu kien cho chai tan thoai ra ngoai lie dang hon. Tuy nhien. do mm eiia nguyen heu ciing phai eo gioi hgn. neu qua mm se lam giam kha nang tham cua dung men vao lop \ ai heu ran do lam tac cac ong mao dan. can tro Uen trinh Inch Iv o min \ ai v i tn. noi ma dung moi khong the tham qua.
Ki'l qua hmh .' cho th.iv kkh thuoc nguven lieu va dung moi chii'l CO anh hmmg den ii le ihu hoi cao chici rong nau a do un civ 'J5''o iloi vol viMiii nu'.v iJdiniiniin ihi n Wh ihuoc U.25 nvi.
\ j duni; moi thiet MeOM cho u le thu liiu v.io ihiel cao hon '•;
V',' uui.'v \ J cac dung inoi ton bl. con o loiij 1,111 .S tioi >nmi thi I' Vwh ihuoc I'.2? -nr.'' v.i o.^ m'"" uui..: nioi \1e('H. DCM 1 lU-\c ^ho tl ^ mu ho 1. j ^ . ;h el khi . ^u <ii '".at ciel i^ JC nnhia -o iXi Ji 1 kich ituoi. -"^ m • i du . m :n el 1;
Mc^Mt chi :a ai n
3 , , ncM dicnioromethane.
Z".. tfiicfi EtOAc ethyl 3cei.iie ^ _ ^ ^ j ^ ^ g ^ g j L .-h fhu -.L nquven lieu [f*i ^ Hinh 3 Anh hu-cvng cu.i kich " " ^ ^ ^ .^,.^ng phaP Soxhlet.
chiet(B)<Ientil?lhahoic.o.t>^ii' . F a,icllidi.gph.nn.gP'>'iP-rJ,"^^''"^"""""
,-,„ •„„ uua 11101 inri-mg lo"g/ran- duai tac Kh, tru>ei, ^>'"_^j;'^^ ; \^,,, „ , , . sy lang ap suat va nhiet 1 " ? " " : : " k " , c^Do do. pha rdn (nguyen lieu) trong moi ''^ " V d r < ^ ' ^ nho. tW dien tich be mat cang lon nen l l T t ^ ^ n ^ r ^ ^ n g eua song sieu am len pha r ^ n c a ^ 1 dim kha nang pha vo ck true te bao cang cao. _Kh. do cac thanh phdn ben trong ti bao hoa tan vao dung moi de dang hon,
„ Ic thu h6i cac ch3l chiel se cao trong tiiai gian ngan. Tuy nhien, ihiT, man chiil phai dii dai de cac chat trong nguyen lieu dupc chiet xuat hoan loan vao dung moi. Khi sir dung song sieu am de ho trg qua trinh chiet, thi y^u td thai gian dugc riit ngan dang ke so vm phumg phap chi^l tmy^n th6ng.
\iec lua chon dung moi hay he dung moi phiii dya vao moi quan he eiira ch^t chilt va dung moi. Khi chatj:hiet da dang thi
\ iec chpn he dung moi la c5n thiai DiSu nay se h6 trg cho qua trinh chii-i lach cac chit chiil cb dp phan cue khac nhau, thay vi chiet phan doan. Sy chenh lech giira li le nguyen heu/dung moi cang lan eang lam lang dien tich tiep xiic giiia nguyen lieu va dung moi, khi do dong lire cua qua trinh khudch tan va hoa tan se cang lan, lam cho cac ihanh phan trong nguyen lieu co xu hudng di vao dung moi nhieu hem. Do do, ti le nguyen lieu/dung moi cang lan Ihi ti le Ihu hdi cao chiet cang cao. Tuy nhien, d ti le chenh lech cang cao thi qua irinh trich ly cang tang dan va dimg lai d mpl ti !e nhat dinh, dong thdi nlu dung mdi sir dung cang nhieu thi qua trinh tach dung mdi de thu nhan cao chiet cang mat nhieu thdi gian, dieu nay se anh huong den chat lugng cao chiet.
Ket qua hinh 4A cho thay kich Ihudc nguyen lieu, dung moi chiei, li le nguyen lieu/dung mdi, thdi gian xij ly sdng sieu am co anh huong den ti le thu hdi cao chiet rong nau d dp tin cay 95%.
0 kich thude nguyen lieu nho nhal (0.25 mm) cho ti le thu hoi cao chiet trung binh cao nhat o ca hai loai rong "1 rong hinh 4B, hdn h^Tp dung mdi DCM/McOll cho ti le thu hdi eao chiet cao nhal d rong mo S" feldmannii. cao hon so vdi mau ddi chieu la chi diing
•'"01 li.ji dung mdi MeOll Tuimg tu. o 5 pulvcvMum ti le thu hdi - :hiet J ca ba loai dung mdi ket hop khong khac hiet d mirc y lu hun khi chi su dung MeOli Dit-u nay cho thiy
>n hap dung
^ vua . iec suduHEikitig mdi ket h(rp Vi
n M MeUH duoL lU Jung i.ho ca hai loai ron'.^ \',nh AC X m .1. Iniannii ihi u le nguyen lieu'dunti ui ^;t,Q ,. i- .L a> Chiet ^^>, nhai ^ " .ur. j S pah ., „ 1; „ i^ , ^^ -^^^^
ron. m , ..xulv .ong ,h^j_ ,^^^
lut cho :i le ihu hni . n^ khdnt: • ha
c;:^.Ar--
Khoa hpc Ky thu$t va Cdng ngh$ i
Sf-S feldmannii, S.p: S. polycyslum, EtOH ethanol. MeOH melhanol, DCM dichlonsmelhane
Hinh 4. Anh hito-ng ciia kich thu'O'c nguyen lieu (A), dung moi chllt (B), ti If nguyen li^u/dung moi (C) va tho-i gian xi> ly song sieu am (0) den ti If thu hoi cao chiet.
95%. De han che sy anh hirmig cua nhiel dp sinh ra do phal sdng sieu am len chat chiel, qua trinh trich ly dirge chpn cho ca hai Ioai rong la 15 phiil, Theo Dina Rodrigues va cdng sy, sir dung sdng sieu am xir ly bpi rong trong nudc hieu suat chiel thu dugc tir 26- 49% [ 11 ]. Sy khac nhau ve li le thu hdi cao chiet tuy thupc nhieu vao dung mdi chiel khi chiel ciing mpt mau nguyen lieu, va viec Ihu cao long trong dung moi co dd phan circ khac nhau tuy thupc
\ao muc dich nghien cuu chiet tach cac nhdm chai khac nhau sau dd.
Chiet ket hi/p .\ir ly enzyme
Cellulose la ihanh phan chii yeu tao nen vd cay rong. Enzyme dirpc sir dung trong nghien cim nay la Viscoenzyme L vdi muc dieh hd trg cho qua trinh thiiy phan lap thanh te bao rong, lam cho cac chat chiet dupc giai phdng de dimg hon, giup lang hieu qua thu hdi chit chiet [12].
Qua bieu dd hinh 5A cho thay d ca hai loai rong li le thu hoi cao chiel d cac kich ihudc khao sat deu cao hon ddi chimg. Dieu nay cho ihay tac dung cua enzyme trong viec hd tro qua trinh chiet.
0 rong ma S. feldmannii thi kich thude 0,25 mm va 0,5 mm cd ti le ihu hdi cao chiel khdng khac biet d muc y nghia 95% va cao hon kich thude 0.63 mm. Cdn d 5. polycystum thi kich thude 0.5 mm cd ti le thu hdi cao chiet cao hem hai tnrdng hgp con lai. Do dd, kich thude chon la 0,5 mm cho ca hai loai rong Hinh 5B cho thay tdc dp phan ung tang ti !e thuan vdi ndng dp enzyme, khi dd thanh te bao bi pha hiiy nhieu. cac chat tan de dang duac chiet xuat ra ngoai. 6 ca hai loai rong ndng dp enzyme 1,5% va 2% co ti le thu hdi cao chiet khdng khac biel a muc y nghTa 95% va cao hon so vdi cac ndng dp con lai. Do dd de giam chi phi, nong dp 1,5%
dirpc chpn de xir ly mau tnrdc khi chiet. Nhiet dp hoat ddng cua Viscozyme L la 25-55°C, hinh 5C cho thay ca hai loai rong mo S.
feldmamni va rong chi S polycystum a nhiet dp 50±2'^^C co ti le thu hoi cao chiei cao nhal. Viec keo dai thdi gian chiet co the gay anh
hudng den dp on dinh cung nhu hoat tinh ciia cac chat chiel, hinh 5D cho thay ca hai loai rong thdi gian chiei 6 gid va 7 gid khdng cd sy khac biet d miic y nghTa 95%. Do do. thdi gian xu ly enzyme Viscozyme L tnrdc khi chiet dugc chon la 6 gid. li le thu hdi cao chiet 7.192-8.020%. So vdi nghien cuu ciia Sabeena Farvin va cdng sy. Dina Rodngues va cdng sy thi hieu suat chiel ihu dugc khi XTJ ly Viscozyme o ndng dp 0,1 % trong dung mdi chiet la nudc dao dpng 37.7-72,7" o: su khac biet ve ti le thu hdi cao chiel nay do su khac nhau ve loai rong nguyen lieu va dung mdi chiet [11. 13].
S f S feldmannii, S p S. polycyslum
Hinh 5. Anh hipo'ng cua kich thud'c nguyen lieu (A), nong dp enzyme (B), nhiet do (C) va thai gian ii enzyme (D) den ti If thu hoi cao chiet.
So sdnh tile thu hdi cao chiet vd ddnh gid khd ndng hdt gdc tir do DPPH ciia cao chiet d- ba phirffng phdp
Tir kel qua nghien ciru cua ba phuong phap chiet tren, chgn dieu kien chiet ldi uu ciia tirng phuong phap. Ket qua so sanh dugc trinh bay trong hinh 6, ti le thu hoi cao chiel d phuong phap chiet sir dung sdng sieu am va phuong phap ngam lac ket hgp xu ly enzyme cao hem so vai phuong phap Soxhiei cho ca hai loai rong. Ve dieu kien thyc hien ciia hai phuong phap nay thi qua trinh chiel cd the tien hanh dugc tren so lugng lon, day cung la uu diem so vai phuong phap Soxhlet. Ti le thu hoi cao chiet ciia ca 3 phuong phap sir dung trong nghien ciru nay dao ddng trong khoang 6,575- 8.373'*o. cao ban so vdi ket qua ciia Hong-Yu Luo va egng sy la 3,34-6.54% [14] va thap han so vdi nghien cim ciia Sujin Lim va cdng sy la 16,7-27,3% [8], dieu nay the hi?n su anh huong ciia dieu kien chiet, phucmg phap chiel va dac biet la ngudn thu nhan nguyen lieu.
C) hinh 7, kha nang bat gdc ly do ciia cao chiet thu bang phucmg phap Soxhlet thap bon so vdi hai p h u a n g phap con lai. do qua trinh nay thyc hien d nhiet dp cao, dieu dd se lam bien doi cac thanh phan nhay vdi nhiel cd trong cao chiet nen anh huong den hoai tinh khang oxy hoa. O phuong phap chiet sir dung song sieu am va xir ly enzyme thi khdng co
I Khoa hpc Ky thuit vi Cdng ngh^
VII k'l.u biC'iiT mu^: \ nghia •J5^B, D o d o . \ei ve hieu qua thu iioi Ji.li chii-i, hai>gdni li le thu nhan cao chiet va kli.i rung quel gtV tg do DPPH thi hai phuijng phap chiet >ir dyng M>ng Mcii am va chiet t>&ng phinrng phap ngam 13c kei hi'p
\lt Iv cn/> me J u . ^ de nghj su dyne cho ddi lu.mg rong nJu Kh.i n3ng k i \ i , j o\y hoa cua cao chiei mng nau danh gia qua kh.i ning hii gt>c ty do DPPH nhin chung thap hiTn so vol eac nghnin cuu dJ cdng Ki [ In. 14. 1 >), M' \<n chai doi
ii.ini ptofK-nie'
-,K IX-M. fv. Mann,
Hlnh 6. So sinh ti If thu h6i cao Hmh 7 So sanh kha nang b^t goc chlAt & ba phuong phdp chl^t. t^r do DPPH cua cao chiet c ba
phirarig phap chi^L
chii-ii kl acid a-corbic ihi chi bing khoang 63%.
t hici k'lng phutTiig ph.ip Sovhlci lot nhal o kich ihirdc bdt rong 0,25 mm. dung mdi ihici McOII va ihm ei.m chiei la 8 gu'i o ca hat I031 mng Ti 1^ thu hoi cao iliici la (\"4-(l.(16(l"o d .V h'liliihin'iii \,i (>.5""^-l),4l V\, (I ,V /.o/i, 1 \iuni Kha nang bai goc III do DPPH tuong ung la .•^-I..'(il"„ \.i 5(>.-'=n3% so vdi chal ddi t lik'ii Ll \ iLiniin t" o cung ndng do
Phuong phap chiel sir dyng song MCU am thuc hien d tan NO 20 kHz: kich thude bpt mng 0,25 mm. hon hgp dung mdi IHAI MfOII ihoca hai loai rong: ti le nguyen lieu dung mdi la 1 ''aS 1,-IJnuiniiu 1 0o,V poit, 1 '•nmi. ihdi gian xu!y la 15 phiil. Ti ll," thu hdi lao chici ia ",|)')^:ll.ll55^• d 5, feklimmiiU
\a S,.''.5;(l,!22"o o V pohi.\\ium Kha n3ng biJl gdc ty do DPPH jumig ling ia (..v0.r% \,i ()2,720"o so voi vitamin C o cung ndng dp.
Phuong phap ngam lac ket hgp xu ly N'isco^me L thu dugc kieh Ihude bpi rong la 0.5 mm. nong dd enzvme 1.5%.
nhi^-t dp u en/yme 50-2 C. ihoi gian u enzyme 6 gid a ca hat loai lotii: Rong sau khi dugc xii Iv, duac chi^t b5ng dung mdi IX"\i McOHd 1), li !e dung mdi nguven lieu la " 1. thdi gian eliii'i la l^ gio Tl li' thu hdt cao chiet lii 7,192-r0.213% „ 5 fcldtnaimii va S.O:n:ii.!s(,% ^ 5 pol\c\sium kha niing hai giK lu do DPPH luimg img la M,(>4:'\ \ a 62.766% so vai V itamin 1," o cung ndng Jo
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