I Khoa hpc Y - Duoc
Hoat tinh khang oxy hoa cua cao chi^t ethanol than re Ngai tim (Kaempferiapar\ifl0ra \M. E.\ Baker),
Ngai tring {Curcuma aromatica Salisb.). Girag gio {Zingiber mmbet Sra.)
Niiuyen Trpng T u a n ' . P h a m Thj Sanh
• ^Khoah,K iif nhun Tnnf-.i: T>ai hoc Can Tho
\gavnhpnbai25 11 201«: ngav ehuven phan b,C-n > 1! 2n|4. ngav nhin phan biv-n 26/12'201'J. nga> chap nh^n dang 31/12/2019
Tdm tdi:
Stress oxy hoa la myt tnin-: nhiniR nguven nhan yiv b9nh t i l cho con nguoi, chii yeu la d o t m h t r a n g m a t c a n b a n g cua c i c c h i t oxy h6a vui cac chat khinsi nvv hoa bao ve trong co- the. Do d(i de nang cao sire khde, hi?n nay, c a c san p h a m nguon goc tir nhien chira cac c h i t k h a n g iivv hoa thirtmg din^c sir dung. C i c cay thuQC h ? G i r n g d a dirp-c biet chii-a nhieu h y p chat k h a n g oxy hoa t\f nhien. \ i v iv. nghien cihi nay nh3ni muc dich d a n h gia h o a t tinh k h a n g oxy htia cua cac cao chiet ethanol than re Nnni lini {Kaempferia par\iftora \ \ a l l . V.\ B a k e r ) , Nf-ai t r a n g {Curcuma aromatic SiA\\\b.) ^-^ C.im^^iQ C^ingiber zeriimbei Sm.\ h^nu cjch sir dung c i c phuffng p h i p thii'nghiem k h i c nhau n h u p h u w n g p h i p D P P H , AB I S' va ninf; li^c k h u (Reducing povvcr). Nynai ra, h a m luffng polyphenol tong v i flavonoid tiing trong eae djch iricli cung Ayrcfc x i c djnh. Ket q u a cho thiiy. c i c cao chi^t deu chfra h a m lirgng loii cac hgrp chiit polyphenol I f i n g v i flavonoid long. Nielli t r i n g v i G i m g g i o i h e hi^n hoat tinh k h a n g oxy hoa tot hoTi so voi Ngai tim trong c i c plimnig p h i p Ihu- nghiC'm. Nghien cuu cho thav tiem niSng ldn (rong viec irng d u n g cac loai thirc val t^r nhien n i y vao Rnh vyx Ih^rc p h a m v i dug-c p h a m .
Tir khdu: (Jinii; nid. k h a n g o w hd;
CIn so phdn loai: 3.4
i tim. Ng
oat vin di
Ngai tim {Kacmplcria/HiniJIora Wali Ex Baker). \i;:ii Iriiiy [Curcuma aivmalic Sahsb.) va Gimg tiio (Zingiber zcrumhei Sin.) mgc hoang hoic dtrgc irong o k h i p ca nuac, lir B i c \.io Nam. d i e bi^'t la cac vimg doi niii [1] Ngjt irang va Ginig gm d i dtroc mo ta trong danh Iyc c i c loai ihiic val
\ liM N,iin [2. 3]. ricne Nuai u'm nicti dugc phit hi^n tr cac unh Dik LLlk. Gia U i (K-Bang) v i Thanh Hoa [4]. Diy la cac civ ihuoc hg Ginig. mgl hg co nhieu irng dyng trong y hgc CO inivcn 11, 2] \\o\ sit nghien cmi irong v i ngoai nuoc cho thiy dav li cac loai thuc val co tiem ning rat ldn irong linh vuc y dirgrc. Ng.u tim co chira 5.7-dimelho\\-flavone CO kha n i n g irc che manh c m / y m c PDE5 va PDE6 diu*u iri roi logn cucmg duong tren chug! [5] cimg nhu l i chai lang cirtmg sire khoe liem n i n g cho nguoi cao luoi do lam giam
^ircss o\v h6.i [6], Cao chiet ethanol Ngai u i n g the hien hoat linh khang viem do co chira lucmg 1cm acid arachidonic [7], d i u ciia Ngai iring co hoai tinh bao ve gan khi uc ch^
Mf phal m e n khoi u ciia gan den 5!-52"q [8]. Gimg gi6 CO kha n i n g giam >u ling trgng lugng c o the. l u ^ g m a n(,ii lang v i nong do lipid huyet luong [9]. Tuy nhien hoai nnh khang o\v hoa cua c i c loii thuc v ai tren con il lii lieu
nghien ciru Chinh vi ihe. irong nghien ciru nay, chiing loi l i p tmng nghien ciru hoat linh khang oxy hoa ciia 3 loai Ihuc vit dugc mo la a tren. Cac loai Ihirc val nay d u g c thu hai iren dia ban Niii Cam vimg Bay N i i , thugc huyen Tinh Bien. tmh An Giang.
fiii tugng va ptiildng phap
^ i-uyeu lieu
Mau nguyen !ieu tuoi (1 kg) mdi loai dirge thu hai tai Niii C i m vung Bay Nil!,huyen Tinh B i e n , l i n h A n G i a n g . Cac m i u dugc Ihu hii tai cac tga do ] a - 2 9 " 4 3 " N ; 1 0 4 ' ' 5 8 ' 5 1 , ! 3 " E a do cao 600 m (Ngai tim); !0''29"60"N. 1 0 4 ° 5 8 ' 4 0 " E a do L a o 4 0 f ) m ( N g a i t r i n g ) v a l 0 ° 3 0 ' 2 4 , 9 8 " N . 104°58'39,55"E o do cao 400 m (Girng gio). Mau d u g c xac nhan boi Phiing T h i H a n g v 6 i s 6 1 i r u m a u N g a i l i m P 1 1 0 2 0 1 7 - A G ( N C ) 0 0 1 3 , Ngai tring PI 112017-AGfNC)0007 va Gimg gio P1072016- AG(NC. \ T ) 0 0 1 7 . Cac mau nay dirge thu hai. dinh danh dua iren dac diem hinh thii va so sanh voi tai lieu chuyen nganh. Mau dugc luu trii lai Phong thi nghiem Smh hpc ihuc
\ a t Bg mon Su pham sinh hgc, Khoa Su pham. Truong Dai hgc Can T h a . Nguyen lieu sau khi thu hii v^ duoc rua sach ioai bo lap ban, cai nho. s i y 6 SC'C v i xay thinh boi.
lbii«GNGNEm vn
28
Antioxidant activity of ethanolic rhizomes extracts ^i Kaempferia
parviflora Wall, Ex Baker, Curcuma aromatica Salisb.,
and Zingiber zerumbet Sm.
Trong Tuan Nguyen', Thi Sanh P h a m College of Natural Sciences, Con Tlio Vnieersit}- Received 25 November 2019. accepled 3! December 2019 Abstract:
Oxidative stress is one of t h e causes of h u m a n s diseases, mainly due to an i m b a l a n c e of oxidative c o m p o u n d s v\'ith protective a n t i o x i d a n t s in the body. T h e r e f o r e , for improving health, n o w a d a y s , n a t u r a l p r o d u c t s c o n t a i n i n g antioxidants a r e often used. Plants belonging to the Zingiberaceae family have been k n o w n to contain lots of natural a n t i o x i d a n t s . Therefore, this research aims to evaluate the a n t i o x i d a n t activities of ethanolic r h i z o m e s extracts of Kaempferia parviflora Wall. K\ Baker, Curcuma aromatic Salisb., and Zingiber zerumbet Sm. by using some different bioassay m e t h o d s such as D P P H , . \ B T S " , and r e d u c i n g power. In a d d i t i o n , the c o n t e n t of total polyphenol a n d total flavonoid in the e x t r a c t s were also investigated. T h e results showed t h a t all of the extracts contained high a m o u n t of total polyphenol a n d total flavonoid c o m p o n e n t s . Curcuma aromatic Salisb.
and Zingiber zerumbet Sm. species provided t h e s t r o n g e r antioxidant activity t h a n Kaempferia parviflora \\"an.
Ex Baker species in all the tested m e t h o d s . In brief, this research reveals a g r e a t potential for application of these medicinal plants in food a n d p h a r m a c e u t i c a l flelds.
Keywords: antioxidant. Curcuma aromatic Salisb., Kaempferia pan-lfiora Wall. E \ Baker, Zingiber zerumbet Sm.
Classification number: 3.4
.Vac dinh do dm bdt nguyen lieu
Do am bgi nguyen tieu dugc x i c dinh theo Dugc diC'n
\'iel Nam \ ' . iheo p h u a n g phap sav [ 10].
Dieu che cao chiet
Bgt kho than re Ngai lim, Ngai trang va Girng gio d u g c chiel vai dung moi ethanol 96° bang phuong p h i p ngam trong 24 gia (3 lan). T i l ca dich chict d u g c gom lai va loc de loai bo can. bui. Dich chiel sau khi Igc dem co quay duoi dung moi o nhiel do 40-45^C thu dugc cao chiel ethanol thin re N'gai tim. Ngai trang va Girng gio. Cao chict dugc dem can v i tinh hieu suit chiel cao. sau do cao dugc bao quan trong lu lanh a nhiet do 4°C cho den khi Ihi nghiem.
Hieu sual chiel cao dugc tfnh theo cong thirc;
% H -Khoi linrng cao chiet X 1 0 0 %
Khao sdt hoat tinh khdng o.\y hda ciia cdc cao chiet Khdo sdt hieu qua loai bo goc tirdo DPPH. kha nang loai bo goc t u do DPPH cua c i c cao chii'l dugc thuc hign Iheo Sharma [ 11 ]. Hon hgp phan iing g6m 40 gl DPPH (1000 pg/
ml) v i 960 gl c i c cao chiel co nong dg khac nhau. Cao chiel Ngai tim c6 nong do 80-560 pg/ml. cao chict Ngai Iring co nong do 30-210 pg/ml va cao chiet Girng gio co nong dg 20- 140 pg/ml. Hon hgp dugc u trong toi o nhiet do 30X' trong thai gian 30 phiil. do dp hap thu quang pho tJ buijc song 517 nm. Doi chimg dtrong su dung ia vitamin C. Ket qua hoat linh khang oxy hoa cua cac cao chiel d u a c bieu dien bang gia in EC,^, (Effective Concentration of 50%) dugc linh dua tren phuong irinh hoi quy tuyen tinh cung nhu h i m lugng chal khang oxy boa luong d u a n g vitamin C (gg/ml) cua dich chiel khao sat. Hieu suit lam sach goc tu do dugc tinh theo cons thirc;
D tre c h e = ^ x 1 0 0 %
Trong do; \^ la gia tri hap ihu ciia DPPH; A^ la gia tn hap thu cua mau ihir.
Khdo sal hieu qud loai bo goc lir do ABTS' • hoat dgng loai bo goc ttr do A B T S " thuc hien theo Nenadis [12].
C h u i n bi dung dich A B T S " bang each iron 2 ml dung djch ABTS 7 mM v a 2 ml dung djch K,S,0^ 2,45 m M . ii trong toi 16 giir. sau do pha loang bang ethanol (khoing 30 lin), dieu chinh do hap thu a hudc song 734 nm dat 0,7±0,05. Tien hanh cho 990 gl ABTS'" v i o 10 pi c i c cao chiel o c i c nong do khac nhau. Cao chiel Ngai iim co nong do 7,5-52,5 pg/
ml. cao Ngai trang co nong do 3-21 gg/ml va cao Girng gio CO nong d o 5-35 pg/ml. Hon hgp phan irng dugc ii irong 6 phiit, sau do do do hap thu quang pho a budc song 734 nm, Doi chimg duong dugc sir dung la Irolox. Ket qua hoai linh
I Khoa hpc Y - Dupc
khang o\v hoa cua c i c cao chiel dugc bieu dien bang gia in I t ^ dugc tinh dya tnin phuang irinh hoi quv luyi-n linh v i ham lir(,Tng chat k h i n g oxy hoa tuong duong tiolox (gg ml) cua dich chiet khao sii Hicu suit lim <jch goc lu do dugc linh theo cong thuc:
% irc c h e • ' ^ c - A s . 1 0 0 %
Trong do' A^ la gii in h i p ihu cua .ABTS' . .-\, l i gia tn h i p Ihu cua mau ihu.
Khan sal naiii; lire khir iBedui nu; Power - RPt RPdugc thyc hign iheo phuang p h i p Ferreira [13]. Hon htip phan irng gom 0..^ ml cac cao o nong do khac nhau lir 100-700 pi; ml. 0.5 ml dvm phosphate in.2 M. p H - 6 . 6 ) va 0.5 ml K 1 c i t N) \"o. Sau do Hon hgp phan iing dugc u o 5()"C I trong 20 phul. them 0.5 ml CCl,COOH 10% roi Iv lim .'^(Klll
v()ngpiuit irong 10 phiu, NIK- nhing rui 0.5 ml djch phia tren cho Vio 0.5 ml nuac v i 0.1 ml FeCL 0 . 1 % . ISc d^u Do dg hap thy quang pho cua hon hgp phan img 6 buoc song 700 nm. C h i i din chirng duong su dyng li irolox, Hicu qua khing o\v hoa cti.i c i c cao chiel o cac nong do khic nhau dugc so sanh vin chat chuin bing each s u d y n g nong dii ma lai do chii chuin hay cao chiel (pg nil) co gia in 0D=il,5 ( 0 1 ) , , t ciing nhu ham lugng chit khang o\v hoa tuong dirong iiolov (pg ml) ciia cic djch chiel.
Djnh lir{mg polyphenol tdng vd flavonoid long Irong cao chiei
H i m lugng polyphenol long trong c i c cao chiei dugc vac dinh b.'ing thuoc thu Folin-Ciocallcu ihco nid la cua Rcbava [14], H i m lugng flavonoid long dugc \ j c dinh iheo phucmg p h i p so miu AlCl cua Bag [15].
Kit Qua
D{i dm bpt nguyen li^'u vd hi^u sudt chiet cao Dl,) i m cua bgt nguven li<'u Ngai um. Ngai trang v a Gimg gio d i dugc xic dmh v i c o g i a l r i lan lugi li 4.''?(->=0.1Siru.
4."4''-(l.!.'2"o v i 4.SSS-1I l2ii"o, C i c gii tri nav cho thiy phii hgp iheo quy dinh ciia Dugc dtcn \ i e l Nam N' [10] (do am bgi nguven lieu --'•>"oi.
Hieu suit chiet cao d.ii lir 9,6 den lO.ZPo.
linh chit dugc irinh bay o bang 1.
Bang 1. Hieu suat. tr^ng thai va mau sac ciia cac c a cac cao co
lO c h i j i
Neai t t ^ g
«(%!
Trfng Ihii VUu t i c
^.inh J Ji. Tim den Sanh die Xanh Jun Sanh lUc N ju den
Hogt linh khdng oxy hda
Hie'u qua loai ho gdc lir do DPPH: ham lugng ehal khane o w hoa iuong duong v itamin C (pganl) cung nhu .'la in EC cu.i cic cao chii*l d u o c linh loan v a trinh bav a bane 2 \ i bing 3
Bang 2. Phan tram irc che va ham luo'ng chat khang oxy hoa hrong duvng vitamin C (pg/ml) ciia cac cao chiet.
, Ham liryiii; chal k h i n g
\&r.Kd9 H i ? u > u a t l i m s ? c h „ „ , |,5„ „ ^ g dinmg
^ " " ^ • ^ (MK'-l) B i c u r d o r / . ) vilamin C (ua/ml) 12.106=0.146 0,973±0.010
;il..557=:ii,1W 1 5JI±O.014 26.OT1±0.241 1,').'5=(U)I7
•i;.7-)'>=0,lW 2,.M4±0,014 4ll,il7(,-0.:5.1 2,898±0.017 4(i,S()2=0.417 3.365±0.029 si.-'4.V 11.096 3.S39±0,007 19,720±0,096 l.497±0,0t)7 32.')4fcl) 526 2.407±0,036 43.135±ii.5SI 3.109±0.04(1 S6.610±0.345 4.l).'7zll,024 66.K68i0.146 4,743^l).01() 77.636±0,345 5,484*0.024 89,965±0.287 6,333*0,020 18,860±0.096 l,438±0,OO7 29.086±0.191 2,142±0,013 41.319±0,253 2,984±0,017 50.l7S±n,146 3.593±0,010 64,097±0,292 4,552±0,020 74.004±0,191 5.234;IM)I3 82,319i0.096 5.806±0,007 Kci q u i o bang 2 cho ihay, ham lugng c h i t khang oxy hoa iuong duong vitamin C (pg/ml) cua cac cao chiet cung lang len khi nong do cao chi^t tang len. Cu thg la a cao chiei Ngai lim ham lugng chal khang oxy hoa t u a n g d u a n g vitamin C (pg/ml) tang tir O,973±O,O10 len 3.839±0,007 p g m l vai hieu qua lim sach g6c lu do DPPH tang tir 12.106=0.146% len 53.743±0.096% a niing do cao chigt tir
^'l-^6^l ug ml, Cao chict Ngai t r i n g lang tu 1.497-0.007 len 6.333:^0,020 jig/ml vai hieu sual lantr lir 19 " 2 0 - 0 0 9 6 %
!en^89.965±0,287% 6 n6ng do lir 30-210 pg,.ml v i cao chict Gimg gio vai hicu qua lam sach goc m do tir lane tir 1 s ^60.0.096% len 8 2 . 3 1 9 . 0 . 0 9 6 % ciing ham luong l u o n g duang lang tir 1,438±0.007 len 5,806±0.007 pt- ml khi n 6 n s d o l i n g III 20-140 pg/ml "
Kin qua linh loan gii in FC„^ o bang 3 chu ih;i, tim (EC. =503.722 pg/ml). cao Ngai "tring il ( '
i OH 984
UMAHOC \ \
tbHOHOHE ' d W
pg/ml) va cao Gimg gio (EC-p=78.729 pg-'ml) la kha lot. dac bigl ia gimg gio (EC <100 pg/ml) deu cao hon vitamin C (EC„=3.58I pg/ml) lan lugt !a 140,649; 30.430 va 21.983 lan. Nhu vay, hieu qua loai bo goc tu do DPPH ciia cic cao chiet dugc xep theo thir tu la cao Gimg gi6>cao Ngai tring>cao Ngii lim. Nhin chung, trong 3 loai thyc vai tren thi Girng gio vi Ngai trang co hoat dgng loai bo goc tu do DPPH l6t vi gia tri EC,,^ thip.
Bang 3. Hieu qua lam sach goc t u do DPPH ciia vitamin C va cic cao chiet.
Phmrng Irinh hoi quy tuyen tinh G i i trj E C ^ (fig/ml) ViiamiiiC y=14,5253x-2,0211
Ngai lim y=0,0916x+3.3397 Njijitrang >=0.4I06\-5,2511 Gimg gio y=0.5749\f4,73B8
3,581 503.722 108.984
Cao chift Nong d^ (|ig/ml)
3U,0 37,5 42,5
Ukm lirgiigchal Hifu suat lAm s|ch khiin): o\s h6a ghe tif do (%) tmmg durmg
truloi mg/ml) 12.934J0.321 2.32l±0,057
4,086±0.033 5.508±0.060 7.531*0.029 9.210±0,092 10,260±[l.060 11,529^0.(144 1,933±0.0!6 3,552±0,016 5.061 ±-.032 6.414±0.057 7.878±0.027 9.057±0,027 10.402±0,027 2.446±0,042 3.9'18^0.089 5.348*0.080 6.734±0.073 8,011±0,032 9.517^0,073 22,S22=0,185
30,786^0,334 42,U7±0,160 5I,523±[1,515 57,402±0.334 64,51 U0.245 I0.75S±0.089 19,826±0.0a9 28.279±0.177 35.86 U0.320 44,l)57-r(l.l54 50,666±U.154 58,197±0.i54 12.633±0.236 22,325*0.496 2'),SS 7^0.445 37 654.-0,41i8 44,Sfl.=;=0,178 53.24 liO.408 63.477*0.496
Ket qua a bang 4 cho ihiy. ham lugng chat khing oxv hoa tuong duong trolox (pg/ml) cua cic cao chiiM cung tang len khi nong do cao chiet tang Icn, Cu the la a cao chiel Ngai lim, him lugng chat khang oxy hoa luong duong irolox (pg ml) tang tir 2.321-0,057 Ien 11,529±0.044 pg ml khi n6ng do cao chiel ling lir 7.5^5 pg/ml. cao chiet Ngai tring tang tir 1,933=0.016 Ten 10.402±0.027 pg/ml khi ling ning dg lii 3-21 ug ml va cao chi^t Girng gio lang lir 2,446±0.042 len
11.345=0.089 pg/ml khi nong dg ling lir 5-35 pg/ml.
Hieu qui lam sach goc lu do ABTS" ciia cac cao chiel va chal chuan dugc trinh bay thong qua gia trj EC,,, suy ra tir phuong trinh hoi quy tuyen tinh tuong img, kcl qui dugc irinh biy 6 bing 5,
Bang 5, Hieu qua lam sach goc ty do ABTS* ciia trolox va cac cao chiet.
P h u v n g trinh hoi quy luy^n tinh Ciil trj EC,, (rig/nil)
Khdo sdt hieu qua loai hd goc lir do ABTS" • ham lugng
chal khang oxy hoa iuang duang trolox (pg/nil) ciia cac cao chiel dugc tinh loan va trinh biy a bang 4.
Bing 4, Hi^u suat lam sach v i him lu'gng chat khang oxy hoa tu'O'ng ducrng trolox (pg/ml) cua cac cao chiet.
Trolox y=5.6014v-0.0687 Ngai tim y=1.3599v+U638 Ngai lijng \ =2.~27\-2,3224 Gimg gio y= 1.7086\+3.2279
S,939 35.838 17.4K4 27,375
Ket qui gii tri EC^^ tit bang 5 cho Ihay, cao Ngai lim (EC,,=35.838 pg/ml). cao Ngai tring (EC„ = 17,484 gg/
ml) va cao Gimg gio (EC-,=27,375 pg/ml) deu the hien kha nang khang oxy hoa rat tot. Tuy nhien, so voi chat chuan trolox thi \ki ban (EC,, =8,939 pg/ml) lin lugt la 4,009;
1,956 vi 3.063 lin.
Tir kel qui iren cho thay, hieu qua loai bo goc tu do ABTS'" ciia cac cao chiet dugc xep theo thir tu la cao Ngai trang>cao Girng gi6>cao Ngai lim, Nhin chung, ca 3 loai thyc vat tren co hoat dgng loai bo goc ly do ABTS" rat tot, gia tri EC,„ thip (<100 pg/ml).
Ndng lire khtr de danh gii nang luc khir, gia tri OD,,,
dugc diing de so sanh vi khi do hap thu cing cao. nang luc khir cang manh. Gii tri OD,^^ cung dugc suy ra lir phuong Irinh hoi quy luyen tinh ciia trolox va cic cao chiet tuong irng. Cac kel qua nghien ciru vc nang luc khu ciia cac cao chiet dugc the hien a bang 6 vi 7,
Bang 6. Nang luc khu cua trolox va cac cao chiet.
Phirong trinh hb'i quy tuyen tinh Gid trj OD^j
G i m g g
>'=0,0124x+0,0ai2 y=O,O009x+0,O933 y=0.0020>.+0,1460 y=0.0012x4-0,0931
33,774 451.889
Ket qua bang 6 cho thay nang lye khir ciia cac cao chiel Ngai tim (OD„,=451.889). Ngai tring (0D„,= I77) va Gimg gio (OD„,=339.083) deu Ihap hon chat chuan trolox (OD„,=33.774) lin lirgt 13,38, 5,241 vi 10.04 lin.
29
I Khoa hpc Y - Dupc
Ngoai gia trj OD„ ihi ham lugng iuang duang chit chuan irolox pg/ml ( T I C l c u j cac cao chiei ciing dugc unh loin va the hii'n 6 bang T
Bang 7 Him liromg chat khang oxy hoa tirong dircmg trolox (pg/
ml) cua cac cao chiet.
N 6 n g d 4 N e i i i i n i Vgai t r i n g G o n g g M 10(1 9.K2.»:I1.352 25.7111:0.213 i:.>»W*:(> Ir.l 200 lfr.415^(iiisi 39.769iO,l23 l'»,661±(1.2!3 lIKI 23.613*0.161 f ^ - l v i l . l M . ;h4'55:*i.iiSI JIKI 1(1,763=11,241. (.•I.ii'l--0.161 5{,4ij->-n;4'.
500 l~,'4'i h.:(H K3.K2K--0.123 44,(.34::0.1M.
600 45.656±0.16K 10I.I'M-.11.I61 <>i.(.|3Hl,l61 700 50,253:(i,:5'» i : 2 " 1 4 H i | y ( . (.•'.5'»I:0.I86 Ki'l qua vi- h i m lugng chii khang o w hoa o bang 7 cho ihay. ham lugng chat khing oxy hoa luong duong trolox (pg/ml) ciia CIC cao chici cimg ting len khi nong do c,u>
chiC'l ling len tir 100-700 pg ml Cy the la o cati cbiei Ng.n lim, h i m Iut.mg chit k h i n g oxy hoa lirtTng dtrong trolox liig ml) l i n g l i r ' l , s : V | i , - ; > : len 5().25.> ^0.:5'l, cao chi^l Ngai tring lung lir 25.7 I0±0,213 len 122.914-ii.ish va cao duct Gimg gio ling lir 12.96S--0.16I len <C 59UII.I86. Tu ket q u i Iron cho tliiv, ning Iyc khu cua c i c cao chiel dugc xep theo Ihir ly ia cao Ng;ii nang -cao Gung gui cao Ngai lim, voi cao Ngai tring co gia Irj UD„, thap ban 200 pg ml.
Dfnh lir^rng polyphenol tdng vd flavonoid tdng H i m lugng polyphenol liing v i flavonoid long trong cac cao chici d u t v vac dmh lutmg ducmg h i m lugng acid g.illic v.i qucrcciin voi plunnig trinh ducmg chuin y 0.()SS1\'(UIS:^1 {R-=0.9993) va v=(MH16"\-() (11126 (k- O.^Hroi Ki-i qti.i dtroc irinh biy a bang S Bang 8. Kel qua djnh luyng polyphenol tong va flavonoid tong ciia cic cao chiet.
Him Itifng polipbmal t£ng TPCltngGAE/Ecaochi^t)
Ham ItrfDg tla%oaoid i6ng TFC (mg QL'g cao chiet]
GimggiP UI4,0:5UI.8^~ •4.::'»:(i,:i5 ki-l qua a bang S cho thav. ham luong polyphenol v i tlav onoid clur.t ii ong Ng.n trang la cao n h i l vcri gia tri lin lui;n li 1 .l.>.246±0,409 mg GAE g v i S1.44.^.- (1.570 mg QE g. kC- den la Gimg gio voi g i i irj luong img I()4.ir5-(),S5~ mg G.AE'g va "4.229r(),215 mg GE'g, cuoi cung li Ngji tim vol (il.5S5^0.3o5 mg C.-\l-. g va 45.124=0.570 mg GE;g.
Ki-l qua n.iv cung phii hgp voi hoat linh khing oxy hoa cua cao cliici Ngai tring va Gimg gio luon lot hon so vai cao
<;o sinh kel qua o b i n g 2 dC'n b i n g S khi nghien ciru vi' N^ai lim cua chiing loi vai cac nghien cuu khac cho [hiy. ham lucmg polyphenol v i flavonoid long ciia eao chiei edianol re Ngai litn d^u cao hon irong nghien ciru n i y (I00.I5=S.34 mg tiAK g v i 104.36=0.43 mg QE g) [16].
Hoat dgng lam sach g6c ly do DPPH cua Ngai lim irong nehien cim cua chung toi cung yen ban ril nhi^u so voi nghien cuu cua Wungsinlavveekul, et al. ( E C , , = 6 I . 5 pg/ml) (1 ~] Tuong ly trong mgl nghien ciru khac ciia Butkhup. et al. voi niing do cao chi^l la 500 pg/ml thi hieu s u i t lic chg l e n d e n M l . M " . [ l 8 ] .
DOI vol Ngai tring. qua kel qua nghien cuu cho thay hoai unh khing oxy hoa cua cao chi£l Ngii t r i n g cung co sy khac biel. Theo nghien cuu cita Lec. et al. tren cao chigt ethanol than re Ngai iring ciia Nhal B i n cho Ihay. cao chiet chi lam sach 27.2% giic tu do DPPH khi a n6ng dg 100 ug ml v i ling Icn 80,2% khi nong dg cao chiSt la 1 nig/ml v i EC,, la 0.27±0.0l nig/ml; a nang luc khu, khi cao chiel a nong do I mg/inl thi do hap Ihu chi dat 0.4 va 0 0 ^ , , len den 2 .^S-0.04 mg/ml. trong khi do nghien ciru ciia chung tt>i gii in EC,,, o phuong phap DPPH la 108,984 pg/ml v i 6 ning luc khu voi gii In OD^,, li 177 pg/ml. Cac kel qua nay cho ihJv rang, hoat linh khing oxy hoa ciia Ngai trang theo nghien cim ciia Loe yeu hon nghien ciru nay rai nhieu [19].
Trong khi do, hoat dgng lam sach goc ty do DPPH ciia cao chiel methanol Irong nghien ciru ciia Al-Reza, et al. lai cho ki-l qua lot hon so vai nghien ciJu ciia chting toi khi gia tri EC,^,dal 16,58 pg/ml [20],
Doi vai Girng gio, h i m lugng polyphenol t6ng va flavonoid ( l 0 4 . 0 7 5 ± 0 , 8 5 7 m g G A E / g v a 74,229±0,215 mg OE g) irong nghien ciru ciia chiing toi cao hon trong nghien cuu cua Nag, et al. (33,64±3,22 mg GAE/g va 26,79±0,68 mg OE/g) va boat tinh khang oxy hoa cung manh han rit nhieu so voi EC,„ trong phuang phap DPPH cua N a g dal 4 r . l 4 pg/ml [21] Trong nghien ciru ciia Bhavesh, el al.. ham lugng polyphenol va flavonoid l6ng lai cao hon (331.93= 1.23 mg GEA/g va 198±2,65 mg QE/g),' tuy nhien hoat unh lam sach goc ly do DPPH va A B T S ' ihi y k han so^voi nghien ciJu cua chung toi voi gii in EC.,, lin lugl la
li -^^^l-^S pg/ml v a 7 8 , 7 2 ± l , l 2 pg/ml [22]
Tai c i sy khac nhau ciia nghien ciru tren co ihc la do su khac bigl vg vi tri dia ly, th6 nhuong va g,6ng thuc val nghien ciru. Ngoii ra, con co the la do khac nhau vg' dun^
moi chiet xuit, methanol la dung moi c6 the chiCn s^^k nhigu thanh phan co hoat tinh sinh hoc ban dung min clhanol luv nhien melhanol hi dung moi co tinh dgc hon ethanol
ISSS{!GSE\k\ani"'^'"°^° 30
K^tluan
Nghien cim khi nang khang oxy hoa ciia cao chiet ethanol than re Ngai lim. Ngai tring va Gimg gio cho thay dugc liem nang khang o\\ hoa ciia cac loai thuc vat nav. Bing each sir dyng cac phuong phap Ihir nghiem hoat linh sinh hgc khac nhau cho nghien ciru nhu phuang phip DPPH. ABTS' vi ning lye khir (Reducing Power), kgl qua cho thiy rang Ngai trang va Girng gio thg hien hoat linh khing oxy hoa ril tot khi cic gii tri EC,,, Ihap, Ket qui nay ciing phii hgp voi vi^c djnh lugng dugc hai thanh phan chinh quan trgng CO lac dung khang oxy hoa manh Ihucrng xual hien trong nhieu loii thuc vit li hgp chat polyphenol vi flavonoid vdi kel qua ham lugng kha cao la 133,246±0,409 mg GAE g v i 81,443±0,570 mg QE/g cho Ngii tring; 104.075^0.857 mg GAE/g va 74,229±0,215 mg GE/g cho Girng gio. Kgi qua nghign ciru the hign ro hoat tinh khang oxy hoa cua cac loai thuc val nay vi la mgl Irong so cic loii thyc vat co liem ning irng dung vao viec phong vi iri benh Ircn ngudi.
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