APPENDIX 2

Glob

APPENDIX 2 APPENDIX 2

STUDIES ON THE LEUKOCYTES IN GEELDIKKOP CASES

(Abbreviations used in the right-hand column are: N = neutrophiles; L = lymphocytes;

M = monocytes; E = eosinophiles; B = basophiles. Data regarding thrombocytes are expressed as "normal number of thrombocytes present, few thrombocytes, etc.". This refers to the number seen on each smear relative to the other cell types when compared with similar smears from the control animals.)

A. Prodromal Cases Sheep No. ' W.C.C.

1 (10'/cu mm)

VB-P.

Differential white cell count and remarks

Hardly any leukocytes could be found in a numbre of stained smears after a most thorough search. Thrombocytes similarly absent

- - - - -- - -- - - - - - - -- - - -- - - -- -- - - -- -- -- - - - -

VB-Q. Definite leukopaenia present. lt was very difficult to count the types of white cells. There were at least 40% of immature neutrophiles and a large number of immature lymphocytes. Very few thrombocytes present

532

Sh N

I w.c.c.

eep o. I (103/cu mm) 1 I

VB-R.

VB-S.

VB-T ..

VB-U.

VB-V ..

VB-W.

VB-X.

B. Early Cases

Sheep No. I W.C.C.

(10³/cu mm) ¹ VB-G.

J. M. M. BROWN APPENDIX 2 (continued)

Differential white cell count and remarks

Too few leukocytes present to perform a proper count. Thrombo- cytes completely absent. A few odd coccoid and bacilloid organisms present

Too few leukocytes present to perform a proper count. All the white cells which were present were highly immature. Most were immature neutrophiles. Lymphocytopaenia. The few lymphocytes present were large ones. Very few thrombocytes present

Too few leukocytes present to perform a proper count. Those present were highly immature and identification was difficult. Most appeared to be immature neutrophiles. Very few thrombocytes present. Numerous small coccoid and bacilloid organisms present N 56%, L 24%, M I 3%, E 7%, B 0%. All leukocytes very immature

especially the neutrophiles. Normal number of thrombocytes N SO%, L 20%, M 12%, E 18%, B 0%. Nearly all the leukocytes

were immature especially the lymphocytes and neutrophiles. Very few segmented neutrophiles present. Normal number of thrombocytes

N 55%, L 20%, M 12%, E 11%, B 2%.

were immature. Very few properly Normal number of thrombocytes

Most leukocytes counted segmented neutrophiles.

N 44%, L 26%, M 13%, E 15%, B 2%. All forms of neutrophiles present, from mature segmented types to unsegmented juveniles.

Lymphocytes and monocytes highly immature. Normal number of thrombocytes

Differential white cell count and remarks

All leukocytes present very immature and difficult to identify. At least 60% were immature neutrophiles. Normal number of thrombocytes

- - - 1 - - - -

VB-H.. I Severe leukopaenia. All white cells present very immature and most

VB-1- : - : - -... 1

VB-J.

VB-K. ... .

I

difficult to identify with certainty. At least 80% were immature neutrophiles, with an almost complete absence of lymphocytes.

Normal number of thrombocytes

All leukocytes present were very immature and most difficult to

identify. At least 70% were immature neutrophiles with relatively few lymphocytes. Normal number of thrombocytes

All leukocytes present were very immature with at least 60% immature neutrophiles and very few identifiable lymphocytes. Normal number of thrombocytes

Marked neutrophilia, mainly juvenile forms present. Lymphocyto- paenia. Normal number of thrombocytes

BIOCHEMICAL STUDIES ON GEELDIKKOP AND ENZOOTIC ICTERUS

Sheep No.

I

₍₁₀^W.C.C.3/cu mm)

I

Vl-I ~~~J-~9

^{_ _}¹

Vl-2 ...

· I · .

^4·75

^I

____ , _ _ _ !

Vl-3 ...

I

^4·35 ^I

Vl-11.

:-:-~~~-~-

APPENDIX 2 (continued)

_ _ _ _ . c : - - -

Differential white cell count and remarks

N 88 %, L 0%, M 12%, E 0%, B 0%. Most neutrophiles very immature. Very few thrombocytes present

- - - -

N 90%, L 4%, M 6%, E 0%, B 0%. Most neutrophiles very immature. Very few thrombocytes present

N 91%, L 4%, M 5%, E 0%, B 0%. Neutrophiles mainly mature segmented types. Normal number of thrombocytes

N 54%, L 30%, M 14%, E I%, B 1%. Neutrophiles mainly mature segmented types. Normal number of thrombocytes

---~---

V1-13. . . 7 ·55 N 77 %, L 5 %, M 11 %, E 6%, B 1 %. A small percentage of immature neutrophiles. Normal number of thrombocytes

V 1-15... 4·65 N 54%, L 18%, M 14%, E 4%, B 0%. High percentage of immature

---! ---

neutrophiles. Normal number of thrombocytes

V 1-17 . . .

I

3 8

I

^Allleukocytes present very Immature and most difficult to identify

I

At least 90% were juvemle neutrophiies. Numerous coccoid and bacilloid orgamsms present. Normal number of thrombocytes

- - - -

Vl-18 _· ___

··_ I

^9·6 ^IAlmost 100% juvenile unsegmented neutrophiles. No thrombocytes could be found

V1-22_._· .. _ .. _. [ ___ 5_·7 _ _ _ ^1' N 51%, L 24%, M 25%, E 0%, B 0%. Large percentage of immature neutrophiles. Normal number of thrombocytes

V 1-23 ... ~- 8 · 75

I

^N^79%,^{L 9}^{%, M}^{11 %,}^{E 0}^{%, B}¹^%. Large percentage of juvenile neutrophiles. Normal number of thrombocytes

Vl-24. :-:-.... .

- _8·8

-_-_I

^N^70%,^L¹⁴^%,^M¹⁶^%,^E⁰^%,^B⁰%. Neutrophiles mainly mature segmented forms. Normal number of thrombocytes

V1-25 ... . 10·65

----1 ---

Vl-26... 8·3

---~---I F-2 ... i 10·2

F-s:-~~~~~--:s:-2--1

I !

C. Advanced Cases-Group l

Sheep No.

w.c.c. I

(10³/cu mm)

N 87 %, L 7%, M 6%, E 0%, B 0%. Most neutrophiles mature segmented forms. Normal number of thrombocytse.

N 88%, L 6%, M 6%, E 0%, B 0%. Most neutrophiles mature segmented forms. Normal number of thrombocytes.

N 78%, L 18%, M 3%, E 1 %, B 0%. Neutrophiles all mature segmented forms. Normal number of thrombocytes.

N 55%, L 36%, M 5 %, E 4%, B 0%. Nothing unusual. Normal number of thrombocytes

Differential white cell count and remarks

VB-L .... 5·2 VB-M ... . 9·2

N 26%, L 30%, M 40%, E 4%, B 0%. Monocytosis? Neutro- philes all immature. Normal number of thrombocytes

N 68 %, L 9 %, M 23 %, E 0%, B 0%. A few immature neutrophiles.

Normal number of thrombocytes

534

Sh N ^'I W.C.C.

eep o . . (103/cu mm) I

VB-N ... 9·4

J. M. M. BROWN APPENDIX 2 (continued)

Differential white cell count and remarks

N 81%, L 10%, M 9%, E 0%, B 0%. Nothing unusual. Normal number of thrombocytes

- - - -

Vl-4 .. 6·4 N 94%, L 5%, M 1%, E 0%, B 0%. Mainly mature segmented

_ _ _ __ _

neutrophiles. Normal number of thrombocytes

Yl-5... 7·8 IN 83%, L 3%, M 13%, E 1%, B 0%. Mainly mature segmented

I

neutrophiles. Normal number of thrombocytes

VJ-6 .. :-:-.~~-l~--l ^N^80%,^{L 5}^%,^M ¹⁴^%,^E¹^%,B 0%. Mainly mature segmented neutrophiles. Normal number of thrombocytes

---: ---~---

N 90%, L 8 %, M 2%, E 0%, B 0%. Large number of juvenile unsegmented neutrophiles. Normal number of thrombocytes.

v

^{l-7 ..} ^3·35

Numerous coccoid and bacilloid organisms

- - - · - - - -

Vl-8. 3·75

Vl-9 ... . 4·9 V1-1L -..

- . f

^7.1

V1-l2. 7·3

- - - -

Vl-14. 11.35

, N 48%, L 11%, M 41%, E 0%, B 0%. Nothing unusual. Normal number of thrombocytes

N 36%, L 22%, M 40%, E 2%, B 0%. Nothing unusual. Normal number of thrombocytes

N 56%, L 22%, M 22%, E 0%, B O%. Nothing unusual. Normal number of thrombocytes

N 63%, L 16%, M 14%, E 7%, B 0%. Neutrophiles mainly mature segmented forms. Normal number of thrombocytes.

N 83%, L 6%, M 7%, EO%, B 0%. Neutrophiles mainly mature segmented forms. Normal number of thrombocytes

_ _ _ _ I ____ _ - - - -- - -- - - -- - - YI-20 ... 1 3·8 N 90%, L 4%, M 6%, E 0%, B 0%. Large percentage of juvenile

neutrophiles. Normal number of thrombocytes - - - -,- - - -

Y1-21... 3·9 N 70%, L 7%, M 23%, E 0%, B 0%. Most neutrophilesjuvenile and unsegmented. Normal number of thrombocytes

D. Advanced Cases- Group 2 Sheep No. W.C.C. I

Differential white cell count and remarks j (10³/cu mm) I

_ _ _ _.__ I

VB-0 .. 4·2

VB-Y. 4·3

VB-ZI. 5 ·I

v

1-16 ... 6·2

N 70%, L 20%, M 9%, E 1%. B 0%. The leukocytes were in general mainly immature forms. Normal number of thrombocytes N 80%, L 15%, M 5%, E 0%, BO%. All leukocytes very immature.

Scarcely any segmented neutrophiles. Reduced number of thrombocytes

, N 76%, L 20 (o, M 4%, E 0%, B 0%. All forms of neutrophiles present, mamly adult forms. Lymphocytes mainly large forms.

Normal number of thrombocytes

N 55%, L 19%, M 18%, E 8%, B 0%. Neutrophiles mainly mature forms. ormal number of tbrombocytes

BIOCHEMICAL STUDIES ON GEELDIKKOP AND ENZOOTIC ICTERUS APPENDIX 2 (continued)

E. Recovered Cases

I

^(l⁰^W.C.C.³^{/cu mm}⁾1

- ---'--

Sheep No. Differential white cell count and remarks

VH-Z ...

I

V1-19 ... .

6·01

5·05

N 65 %, L 25 %, M 5 %, E 5 %, B 0%. All leukocytes very immature and difficult to identify with certainty. Neutrophiles were mainly unsegmented forms. Some myeloblasts present. Very few throm bocytes evident

N 38 %, L 50%, M 3 %, E 9%, H 0%. Nothing unusual. Normal number of thrombocytes

F-3... ... 8·35 N 78%, L 12%, M 10%, E 0%, H 0%. Neutrophiles mainly mature 1 forms. Normal number of thrombocytes

.f4.~.~~-:-l-3.so

___

N 86%, L 5%, M 7~{, E 2%, B 0%. Neutrophiles mainly mature segmented forms. Normal number of thrombocytes

D. Control Animals

Sheep No. Vl-7054-7057, V1-7059-7062, V1-7064-7071: White cell counts ranged from 4·9 X

10³/cu mm to 9 ·15 x 10³/cu mm. Differential white cell counts varied little around a mean of N 35%, L SO%, M 4%, E 8%, H 0%.

Sheep No. 12221 and 12222: White cell counts were 3·5 X 10' and 3·6 X 10³/cu mm respectively, Differential white cell counts were N 54%, L 30%, M 8%, E 8

%,

B 0% and N 56%, L 41%.

M 3%, E l% and H 0% respectively.

E. Normal Values for Leukocytes of the sheep taken from the literuture

Determination Values Sources

White cell count ... I 4-12 X 10'/cu mm ... I Schalm (1961, 1965) 8-10 x 10³/cu mm ...

I

^Jack^son⁽¹⁹⁴⁸⁾

Differential

count white cell

6· 1-12·2 X 10³/cu mm (9·2).. Boddie (1946) N 30%, L 62 %, M 2 · 5 %, E 4 · 5 %,

B 0·5%

N 30%, L 55%, M 4%, E 8%, B 0·5-1%

1 N 15-23 (24)%, L 58-78 (68)%, ₁ M 1·3-5·7 (3)%, E 0·5-8·5 (4)%,

HO% I

N 30-40%, L 45-70%, M 2-5%,

I

E 5-15%, Bless than 1%

Schalm (Average values) (1961, 1965)

Jackson ( 1948) Boddie ( 1946)

Scheunert & Trautmann (J 965)

Note: (a) The values given by Jackson (1948) are compiled from figures on South African sheep.

They have been used for comparison in the text together with those from our own control animals.

(b) Figures given in parenthesis in the middle column above represent mean values given by the authors concerned.

536

APPENDIX 3 STUDIES ON THE ERYTHROCYTES IN GEELDlKKOP CASES [Note.-RCV = Red cell volume(%); Hb = haemoglobin (gm%); RCC = Red cell count (JO"/cu mm); ESR =Erythrocyte sedimentation rate (mm/hr); TPP =Total plasma proteins (gm%); MCHC =Mean corpuscular haemoglobin concentration(%); MCV =Mean corpuscular volume (cp.); MCH = Mean corpuscular haemoglobin ( yy).] A. Prodromal Cases Sheep No. RCV RCC Hb TPP VB-P Not done I Not done

I

Not done I 4·69 VH-Q

I

Not done 1 Not done I Not clone

I

4 · 69

-- ESR MCHC MCV 0 0

MCH Blood and Bone Marrow Smear Examination-Remarks Blood smear: Very marked anisocytosis, large number of macrocytes. Anaemia Blood smear: Fair degree of anisocytosis, large number of macrocytes. Anaemia ---·--- VH-R Not done

I

Not done

I

Not done

I

4·59 1 0 VB-S 5·33 Not

don~ l

Not done

I

Not done

~ ----

0 VB-T Not done Not done

I

Not done 4 69 1 0

- VB - ~L JT N~~ ~

Not done

I

Not clone! 5·01 __

o _ . __ _

VH-V Not clone

I

Not done Not done

I

5·12 0 Blood smear: Fair degree of anisocytosis, mild anaemia Blood smear: Severe anisocytosis with numerous large macrocytes. Nu- merous cells present with Jolly bodies Blood smear: Fair degree of anisocytosis with numerous large macrocytes. Numerous cells present with Jolly bodies Blood smear: Mild anisocytosis

I

^Blood

smear: Pronounced anisocytosis with large number of macrocytes. Numerous cells with Jolly bodies. --- VH-W Not clone Not done

I

Not done 4·8

v~ l

Not done

I

Not done I Not done

I

5·01

0 1 0

Blood smear: Marked anisocytosis with numerous large macrocytes. Numerous normoblasts and cells with Jolly bodies Blood smear: Fair degree of anisocytosis with large numbers of macrocytes. Large numbers of norma- blasts and cells with Jolly bodies

:-- 3: 3:

1:0 ;o 0 ~

z

VI VJ 00

APPENDIX 3 (continued) STUDIES ON THE ERYTHROCYTES IN GEELDIKKOP CASES (continued) B. Early Cases --- ESR Sheep No.I -'---

I

MCHC ·----'-

RCV RCC Hb TPP VB-0

I

Not done : Not done 1 Not done J 6 · 83 0 VB-H j Not done 1 Not done ' Not done 5 · 33 I 0 VB-I I Not done I Not done 1 Not done 5 ·55 1-1·0

~ -

Not done I Not done I Not done j 5 · 97 ,-0 VB-K I Not done 1 Not done 1 Not done

~ - 5 ~ -7 -~- 0

V1-1 I 46 10·03 11·1 I 7·60 I 0 1_24_· __ : V1-2 I 49 11·85 10·86 I 7·75 0

I

22·16 Vl-3 I 41

I

12·38 10·13

I

7·95 0

I

24·7 --- YI-11 I 35·5 8·93 9·65 I 7·75 0·5 27. 18

MCV 45·86 41· 35 33.11 39·75 vt-13

- ~ ~ --

10·79 9·89 1_ 7·20

i _ o _ _ l ~~ -~ - 41·70

Vl-15

I

46 6·72

I

10·62 8·3

I

23·08 I 68·45 Vl-17

I

49·5 ll-47 12·78 ,--s-.4-1 0

I

25·81 I 43·15 V1-18 I 45·5 9·69 11·10

I

8·4

I

24·39

I

46·95 V1-22 I 48·5 I 10 21

I

10·86

I

5·9 II 0 I 22·39

I

47·50 VJ-23

1 41~ -

10·12 I 9·65

I

5·6 0·5

- 1

23·53 I 40·51 VJ-24

1 ---.,:o - 1 ~' -5 - ~

LJ 82

I

6 4

i

0 5

! ~ i

44 23-

MCH 11·06 9·16 8.18 10·80

I

Blood and Bone Marrow Smear Examination-Remarks Blood smear: Fair degree of anisocytosis Blood smear: Mild anisocytosis

I

Blood smear: Nothing unusual Blood smear: Nothing unusual I I Blood smear: Nothing unusual

Nothing unusual Blood smear: Blood smear: Nothing unusual Blood smear: Fair degree of anisocytosis Bone marrow smear: Apparent reduc- tion in the number of erythrocyte and granulocyte precursors Blood smear: Mild anisocytosis with some macrocytes 9·16

I

Blood smear: Mild anisocytosis 15·80 Blood smear: Nothing unusual 11 ·14

I

Blood smear: Nothing unusual 11 · 45 Blood smear: Nothing unusual 10·63 Blood smear: Nothing unusual 9 ·53 Blood smear: Nothing unusual 10·89 Blood smear: Nothing unusual Bone marrow smear: Bone marrow highly active

0

(")

::r:

tT1 ~

0

)> r' C/1 -1

c

0 ... [T1 C/1 0

z

Cl tT1 ^[T1

G

r:

0 "d )>

z

0 iT1

z

N 0 0 -1 ... (")

a

iT1 ;;.:!

c::

C/1

APPENDIX 3 (continued) STUDIES ON THE ERYTHROCYTES IN GEELDIKKOP CASES (continued) Sheep No.I RCV 1 ----~----~----

RCC Hb TPP V1-25 48·0 12·50 11 ·10 6·9 V1-26 48·0 9·86 11·82 6·9

ESR MCHC 0 23 ·12 0·5 24·62

MCV 38·40 48·68

MCH Blood and Bone Marrow Smear Examinatoin-Remarks 8 · 88

I

Blood smear: Nothing unusual 11 · 98

I

Blood smear: Nothing unusual F-2 1 31·0 3·1

I

8·93 1 6·5 0 I' 28·80 100·0

I

28·80

I

Blood smear: Nothing unusual F-5 36·5 4·79

I

11·10 6·4 0 30·41 76·20

I

23·17 Blood smear: Nothing unusual V3-6 39 Not done 9 · 15

I

8 ·42 0 23-46 --Blood smear: Nothing unusual V3-7 35 Notdone 8·44

I

10·19 0 24·11 --Bloodsmear: Nothingunusual V3-10 1

27 Not done 5 · 70 8 · 08 0 · 5 21 · 11 --

I

Blood smear: Nothing unusual I ---

I I

~-40

I

Not done 8·69 I 10·60 1·0 21·72 --Blood smear: Nothing unusual ---l I I I

~

V3-13

I

Not

done ~ ~

V3-14

I

46 Not done

I

1 I ·I V3-15

I

46 I Not done I

1 0 ~ V3 ~ ~

I

Not done

I

7 · 24 V3-20 42 I Not done 1 8 · 3 V3-21 41 ·Not done

I

8·69

V 3 ~ ~

41 Not done

l

9·6S V3-23 45 Not done

I

9 · 9 V3-24 I 46 I Not done

~~ 5

8·08 [ 0·5 1 20·97 V3-25 [ 34 Not done

I

7 · 24 -8

~ ~

V3-26 I 49 Not done

I

10·8 \ 8·80 I 0

[ --z3.47

V3-27 -~ 32 Not done I 7·72

I

7·56

I

0 24·12

I

I 6 82 0 27·4 1 Blood smear: Nothing unusual

I

Blood smear: Nothing unusual I Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual [ Blood smear: Nothing unusual Blood smear: Nothing unusual I Blood smear: Nothing unusual Blood smear: Nothing unusual I

8. 17 0 24·13 7·90 0

I _ ::~

9·28 0·5

I

28·96 8 60 0 19·76 9·65 0 1 21· 19 7·29 0

1--=:. ~ .

7·56 0·5 I 22·0 ---

:-

~ ~ l:d :::0 0 ~

z

Vl ~

APPENDIX 3 (continued) STUDIES ON THE ERYTHROCYTES IN GEELDIKKOP CASES (continued) C. Advanced Cases-Group 1 Sheep No., RCV RCC Hb TPP VB-L Not done Not done

I

Not done 7·47 VB-M Not done Not done Not done 6-4 VB-N Not done Not done Not done 7·47 Vl-4 33 7·57 I 7·72 8·4 V1-5 41·5 11·47 9·65 7·95 V1-6 36 10·61 7·96 9·6 V1-7 38 I 2·04 8·93 8·4 --- V1-8 35 8·27 9·41 7·75

-- - -- --- - -

V1-9 28 9·1 8·2 7·6

I

V1-10 42 12·28 9·65 9·6 V1-12 22·5 7·18 6·27 8·1 V1-14 30 6·83 7·24 8·8 I V1-20 34·0 10·65 9·17

I

7·3 I

I I _ !(}~ -'

V1-21 44·5 8·78 6·3 V3-1 31 Not done

I

6·75

I

9·84

ESR

I

MCHC

I

MCV 0 - I

I

1·0 -

-

- 2·0 23·39 43·59 0 23·25 36·18 0· 5 22·1 33·93 0

I

23·5 186·27 0·5 26·88 42·32 0 29·28 30·77 --- 0 22·97 34·02 0 27·86 31·33 2 24·13 43·9 1·0 26·97 31·92 0 23·30 50·68 I 0

I

21·77

I

MCH

- -

- 10·19 8·41 7·50 43·77 11· 37 9·01 7·85 8·73 10·60 8· 61 11·81 -

Blood and Bone Marrow Smear Examination-Remarks

I

Blood

~mear:

Anisocytosis, polychro- masm Blood smear: Nothing unusual Blood smear: Nothing unusual

I

Blood smear: Slight anisocytosis

I

Blood smear: Slight anisocytosis Blood smear: Slight anisocytosis Bone Marrow smear: Decrease of erythrocyte and granulocyte precursors. No normoblasts to be seen Blood smear: Nothing unusual 1 Bone Marrow smear: Marked decrease of all cellular elements Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Marked anisocytosis

I

Blood smear: Nothing unusual Blood smear: Nothing unusual Bone Marrow smear: Very active haemopoiesis Blood smear: Nothing unusual Blood smear: Nothing unusual

t::d

0

(")

::r: m

~ ... (") ;J> l' en ....,

§

...

m

en 0

z

Cl tT1 tT1

G

~ ~ 0 "ti

> ~

tT1

z

N 0 0

::l

(")

~

c::

-""

APPENDIX 3 (continued) STUDIES ON THE ERYTHROCYTES IN GEELDIKKOP CASES (continued) RCC Hb Sheep No.I RCV ---';---,-- V3-IA

I

Not done

I : ----

V3-2

I

Not done

I

9· 65 V3-8

I

23 1 Not done

I ~

V3-9--40

I

Not done

I

9· 65

-- ~: - ~: - ~ -- - 1--- : -: ---

V3-18

1 2)

I Not done --- Il·l

I

Not done 5·3

I

Not done 5·79 D. Advanced Cases-Group 2 Sheep No.I RCV RCC Hb VB-0 Not done

I

Not done

~ ~

VB-Y Not done

I

Not done 1 N VB-Z1 j Not done

I

Not done

I N

I .

• 31 1 7·3 -

I

I ot done I

~ I

done\ --- ·24

I

V1-16

TPP I ESR I

MC~ ~

MCV I MCH

I

Blood and Bone Marrow Smear Examination -Remarks ---- 8·8

I

---

I

I I

8·60 0·5 22·44

-

___ I - 8·42 1 0

I

23·47

I

7· 34

1 o

1 24· 12

1 - 1

8-6_1_0_1_24·66 -~--=-1 5·46

I

22·08 I I

I

---

i I I

11·10 0 25·17 - TPP ESR I MCHC I MCV 6·4 I 1·0 -I -

-

- - - MCH

Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual

I

Blood smear: Nothing unusual

I

Blood smear: Nothing unusual

I

Blood smear: Nothing unusual Blood smear: Nothing unusual Blood and Bone Marrow smear Examination-Remarks

- I

Blood smear: Nothing unusual 5·87 I 0

- ~ -=- --=- 1

Blood smear: Nothing unusual --- 5·33 0 -

-

-Blood smear: Marked anisocytosi with numerous macrocytes and cell containing Jolly bodies

I

6·5 1·5 23·35 42·46 9·92

I

Blood smear: Nothing unusual Bone Marrow smear: Markedly re duced number of cellular element I s

~ ~ ~ t:d ;;o 0 ~

z

(../)

""'"

APPENDIX 3 (continued) STUDIES ON THE ERYTHROCYTES IN GEELDIKKOP CASES (continued) E. Recovered Cases Sheep No.I RCV RCC Hb TPP ESR I MCHC MCV MCH Blood and Bone Marrow Smear Examination-Remarks VB-Z Not done

I

Not done Not done I 5·44 I 0 --

I -

Blood smear: Nothing unusual V1-19 47·5 I 13·55 10·62 I 7·3 0·5 22·35 35·05 J 7·83 _!!load smear: Nothing unusual F-3 36· 5 12· 4 11· 58 7· 5 0 31· 72 29· 43

I

9· 33 Blood smear: Nothing unusual -F-4

-- ~36.1 - - ---;{; -g - ~

9·65 7·26 --2--o--J26"03 134·70 36·00 I Blood smear: Marked anisocytosis i , , with large number of macrocytes V3-3 V3-4

51 --

Not done

'~ -- r

8·26

1- o -- i

17·84 I -I -

i

Blood smear: Nothing unusual 40 Not done 8·93 1---;5_6_

0 --= ~ - 22·32 _I - I -

I Blood smear: Nothing unusual V3-19 32 Not done I 7· 7 : 5· 30 I 0 24· 06 --I Blood smear: Nothing unusual F. Control Animals Sheep No.[ RCV

I

RCC

I

Hb TPP

I

ESR MCHC Y1-7054 I 27 8·8 I 8·68 I 6·05 I 1·0 V1-705s_J=29= 9·93

~ - ~ - ~ - 6·4 = ~--

V1-7056 I 32 10·02

I

9·17

~ - 1

V1-7057 I 35 10·6 I 9·65

i

6·55

32·1 =I 28·27 I 28·65 1·0 I 27·57 Y1-7059-i 36 I 13·42 I 10·62

~-~- ~-

0--~29~- Yl-70WI 33 I 11·5

I

9·17

~~ -1

V1-7061 I 35 I 10·36 I 9·41 . 6·1

1 -

--- V1-7062 V1-7064

1 3o ·1 30

I

11· s1 I 10· 83 8·93 6·8 8·68 6·6

0·5 I

27·78 1·0 26·88 0· 5 29·76 0·5 28·93

MCV 30·68

I

29·20 I 31·37 33·02 I -26·82 1 28·69 I 33·78 25·93

I

27·70

MCH 9·86 8·25 8·99 9·10 7·91 7·97 9·08 7·72 8·01

Blood and Bone Marrow Smear Examination-Remarks Blood smear: Nothing unusual j Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual Blood smear: Nothing unusual ·-· --

t:d ... 0 ()

F;i a:: ~

!:"' en

..., §

... t'T1 en 0

z

0 t'T1 t'T1 r-' lj ~ :;><:: 0 >ti )>

z

lj t'T1

z

N 0 0

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APPENDIX 3 (continued) STUDIES ON THE ERYTHROCYTES IN GEELDIKKOP CASES (continued) F. Control Animals Sheep No. I RCV RCC Hb TPP ESR MCHC MCV MCH Blood and Bone Marrow Smear Examination-Remarks Vl-7065 1 33 13·46 9·17 6·6 0·5 27·78 24·51 6·81 I Blood smear: Nothing unusual I vl-7066 -~-ls---~-J0~4-,-s:2--

1

-6~--~-2-. 0--~--ms-27 · 34 1---g.Ql-l Blood--:mear::--N~~~~~--

V1-7067 = ~- 3 1 === 1 ~ ~~ -=

8·68

- 6~ -- 1

I·0--1-28·0-==29·02

- ~ -- 8-:t2 - ~

Blood smear: Nothing unusual V1-7068 1 31 J Not done 9·65 1 Not done 1 1·0 1 31·12 1 -1 -

I

Blood smear: Nothing unusual Vl-7069_;_32~--~ Not done -9~-:Notdone ~-l~---JO~J-1--=--,--=--s/o";;d--;mear::-Nothin~~sual -- Vl-7070-1--:;;---INot don~l 10·13 1Notdon~l 0 I 34·93 ,--=-~---=--1 Blood smear: Nothing unusual

:t:

VI-70nj-30 ___ jNot

d; ; ; : ;~ - ~ - 9~ 7- 1 Notdo ~ ~

0·5 1 30·56 ;--=-:--=--,Blood smear: Nothing unusual ------1 F-12221 I 34· 8 l 11· 54

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9· 65 1 __

6 ~ _3 _ :

0 : 27· 72

1 _ 30 ~ 1

8· 36 I Blood smear: Nothing unusual F-12222-i 25 1 6·00 1 9·65 1 6·0

I

0 I 38·60 I 41·66 16·08 I Blood smear: Nothing unusual F-K2--~-2s~--~-JI~r-:--7-·-7Z-,-5-. 6-4--

1

-o--~-3o0s_/ __ 21-·-2s_

1

_6~3-\s-lo_o_d --;,-ne_a_r::-No_t_h-in_g_u_n-us_u_a_l -- V3-.:._ __

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~,- ~~ - r- _2 ~ __

1 0· 5

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24· 12

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=!--=--' Blood smear: Nothing unusual V3-28 f 30 1 Not done 8· 2 8· 8

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0 I 27· 33 J -

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Blood smear: Nothing unusual v~:-34---1 Not done

1

--s.'69_1_9~1--0 ---~~s-1 -

i -

Blood smear: Nothing unusual

-- --- - -- ' -- ---

---·---- V3-30 ! 30 I Not done

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8·44 I 7·9

I

28·13

I - I

I Blood smear: Nothing unusual

V3- -3 1 -- l - 3s -- ~~d~ ~ - 9~s - ~ - S~ -

0 27· 57 -

I -

Blood smear: Nothing unusual V3-32 _!_:..'_ __ _!Not done

I

8·93

I

7·99

I

28·80

~ ---=-- ~ = - ~ Bl oo d

smear: Nothing unusual V3-33 I 19

I

Not done

I

5·79

I

9·28 0 \ 30·47 I -

I - I

Blood smear: Nothing unusual I I I I I ---~----

~ ~ ~ ttl ;o 0 ~

z

APPENDIX 3 (continued) STUDIES ON THE ERYTHROCYTES IN GEELDlKKOP CASES (continued) G. Normal Values for the Erythrocytes of the sheep taken from the literature Hb ESR I MCHC

I

MCV I MCH Source -I -

I - I

----1---,---,---1 I Jackson (1948) 1 33· 5-43 ----11----9-14·5 0 33-35 9-13 Coffin (1953) 11-13·8 (12·4) 37·1-45·1 (41) 1 23-31 (27) Boddie (1946)

____ _

_

__ _ _ __ _ _

_ ___ _

_

, 10-15 (12·6)

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- 129-35 (32) I - I -Scheunert & Trautmann (1965) 8-16 (12) 0 23-48 (33)19-12 (10· 7) Schalm (1961, 1965) -

I

- I -

!

Reda & Hathout (1957)

I

I -- --

-

Note.-The average value for RCC given by Jackson (1948) has been taken for comparison in the text since it has been worked out on South African sheep. Values in parenthesis in the columns above represent mean values given by the authors concerned.

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J. M. M. BROWN

Dalam dokumen Biochemical studies on geeldikkop and enzootic icterus (Halaman 99-112)

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APPENDIX 2 APPENDIX 2

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