Chapter 3: In vitro antioxidant, anti-hypergycaemic and cytotoxicity activity of Albizia
3.2 In vitro α-amylase and α-glucosidase inhibitory effects and cytotoxicity activity of
3.2.6 Discussion
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In order to ascertain the safety of the extracts, the cytotoxicity activities of A.antunesiana extracts were investigated and we found out that aqueous leaf and aqueous root extracts showed a significant cytotoxicity activity as confirmed in Fig. 3.6 by the reduction in cell viability by approximately 51 and 53% respectively at 200 µg/ml concentration. However the ethanol root extract did not significantly decrease the viability of cells. Therefore, it necessitates the further investigation of the aqueous extracts to identify the responsible compounds for the observed toxic activity and further assess their chemotherapeutical properties. Also further aiming to identify the bioactive compounds of the ethanol root extracts so as to separate them and realise the full therapeutic potentials of the bioactive components.
Based on the results presented in this section, it can be concluded that ethanolic root extract of A.antunesiana exerts an inhibitory effect on α-glucosidase and α-amylase and also shows to be relatively non-toxic at tested concentrations. Furthermore, the results displayed by the ethanol root extract are interesting enough to be used in in vivo models. The obtained data further agree with logic behind the traditional use of plants in medicine based on their inhibitory activity of glucose absorption in the gut.
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