PAGE - A phytochemical investigation of two South African plants with the screening of extracti

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158

COMPOUND 3: 4'-DEMETHYL-3,9-DlHYDROEUCOMIN

Spectrum 3a: IH NMR spectrum of compound 3 (CDCh) Spectrum 3b: l3C NMR spectrum of compound 3 (CDCh) Spectrum 3c: HSQC spectrum of compound 3

Spectrum 3d: HMBC spectrum of compound 3 Spectrum 3e: COSY spectrum of compound 3 Spectrum 3f: NOESY spectrum of compound 3 Spectrum 3g: Mass spectrum of compound 3

Spectrum 3h: UV spectrum + UV (+NaOAc) spectrum of compound 3

Spectrum 3i: UV spectrum + UV (+AICh) spectrum of compound 3

Spectrum 3j: IR spectrum of compound 3

COMPOUND 4: 4'-DEMETHYL-5-0-METHYL-3,9-DlHYDROEUCOMIN

Spectrum 4a: IH NMR spectrum of compound 4 (CDC h) Spectrum 4b:^l3C NMR spectrum of compound 4 (CDCh) Spectrum 4c: HSQC spectrum of compound 4

Spectrum 4d: HMBC spectrum of compound 4 Spectrum 4e: COSY spectrum of compound 4 Spectrum 4f: NOESY spectrum of compound 4 Spectrum 4g: Mass spectrum of compound 4

Spectrum 4h: UV spectrum +UV (+NaOAc) spectrum of compound 4

Spectrum 4i: UV spectrum + UV (+AICh) spectrum of compound 4

Spectrum 4j: Infrared spectrum of compound 4

Spectrum 4k: IH NMR spectrum of compound 4 (CD₃0D) Spectrum 41: l3C NMR spectrum of compound 4 (CD₃0D)

159 160 161 162 163 164 165 166

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GRAPHS SHOWING ST ANDARD DEVIATION

Graph

1:

Graph of varying concentrations ofluminol solution.

Volume of blood cells and opsonized zymosan stimulant were kept constant at 400uL and 1 OOuL respectively, ImM luminol solution Graph 2: Graph of varying concentrations ofluminol solution.

Volume of blood cells and opsonized zymosan stimulant were kept constant at 400uL and lOOuL respectively, 3mM luminol solution Graph 3: Graph of varying concentrations ofluminol solution.

Volume of blood cells and opsonized zymosan stimulant were kept constant at 400uL and lOOuL respectively, 5mM luminol solution Graph 4: Graph of varying concentrations ofluminol solution.

Volume of blood cells and opsonized zymosan stimulant were kept constant at 400uL and 1 OOuL respectively, 12mM luminol solution Graph 5: Graph of varying volumes of luminol solution, opsonized

zymosan, and blood cells. 400jJ.L blood cells, 50jJ.L luminol, 50jJ.L zymosan

Graph 6: Graph of varying volumes ofluminol solution, opsonized

zymosan, and blood cells. 400jJ.L blood cells, 100jJ.L luminol, 50jJ.L zymosan

Graph 7: Graph of varying volumes ofluminol solution, opsonized

zymosan, and blood cells. 400jJ.L blood cells, 100jJ.L luminol, 100jJ.L zymosan

Graph 8: Graph of varying volumes ofluminol solution, opsonized zymosan, and blood cells. 500jJ.L blood cells, 50jJ.L luminol, 50jJ.L zymosan

Graph 9: Graph of varying volumes of luminol solution, opsonized zymosan, and blood cells. 500jJ.L blood cells, 100jJ.L luminol, 50jJ.L zymosan

Graph 10: Graph of varying volumes ofluminol solution, opsonized zymosan, and blood cells. 500jJ.L blood cells, 100jJ.L luminol,

100jJ.L zymosan

Appendix

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Graph 11: Graph of anti-inflammatory activity of compound 2.

SO)lL of compound 2 at Smg/mL concentration was added Graph 12: Graph of anti-inflammatory activity of compound 2.

SO)lL of compound 2 at 10mg/mL concentration was added Graph 13: Graph of anti-inflammatory activity of compound 2.

SO)lL of compound 2 at 20mg/mL concentration was added Graph 14: Graph of anti-inflammatory activity of compound 2.

SO)lL of compound 2 at 40mg/mL concentration was added Graph 15: Graph of anti-inflammatory activity of compound 3.

50)lL of compound 3at Img/mL concentration was added Graph 16: Graph of anti-inflammatory activity of compound 3.

50)lL of compound 3at Smg/mL concentration was added Graph 17: Graph of anti-inflammatory activity of compound 3.

SO)lL of compound 3 at 10mg/mL concentration was added Graph 18: Graph of anti-inflammatory activity of compound 3.

SO)lL of compound 3at 20mg/rnL concentration was added Graph 19: Graph of anti-inflammatory activity of compound 3.

SO)lL of compound 3at 40mg/mL concentration was added Graph 20: Graph of anti-inflammatory activity of compound 4.

50)lL of compound 4at Smg/mL concentration was added Graph 21: Graph of anti-inflammatory activity of compound 4.

50)lL of compound 4at 10mg/rnL concentration was added Graph 22: Graph of anti-inflammatory activity of compound 4.

SO)lL of compound 4at 20mg/mL concentration was added Graph 23: Graph of anti-inflammatory activity of compound 4.

SO)lL of compound 4at 40mg/mL concentration was added

Graph 24: Results of Antioxidant! free radical scavenging activity of compound 2, O.Smg/mL concentration

Graph 25: Results of Antioxidant! free radical scavenging activity of compound 2, Img/mL concentration

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191

Graph 26: Results of Antioxidant! free radical scavenging activity of compound 2, 5mglmL concentration

Graph 27: Results of Antioxidant! free radical scavenging activity of compound 2, 10mglmL concentration

Graph 28: Results of Antioxidant! free radical scavenging activity of compound 3, O.5mglmL concentration

Graph 29: Results of Antioxidant! free radical scavenging activity of compound 3, 1 mglmL concentration

Graph 30: Results of Antioxidant! free radical scavenging activity of compound 3, 5mglmL concentration

Graph 31: Results of Antioxidant! free radical scavenging activity of compound 3, 10mglmL concentration

Graph 32: Results of Antioxidant! free radical scavenging activity of compound 4, O.5mglmL concentration

Graph 33: Results of Antioxidant! free radical scavenging activity of compound 4, ImglmL concentration

Graph 34: Results of Antioxidant! free radical scavenging activity of compound 4, 5mg/mL concentration

Graph 35: Results of Antioxidant! free radical scavenging activity of compound 4, 10mglmL concentration

Graph 36: Results of Antioxidant! free radical scavenging activity of meloxicam, 1 mglmL concentration

Graph 37: Results of Antioxidant! free radical scavenging activity of meloxicam, 5mglmL concentration

Graph 38: Results of Antioxidant! free radical scavenging activity of meloxicam, 10mglmL concentration

Graph 39: Results of Antioxidant! free radical scavenging activity of diclofenac, ImglmL concentration

Graph 40: Results of Antioxidant! free radical scavenging activity of diclofenac, 5mglmL concentration

Appendix

191

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195

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198

Graph 41: Results of Antioxidant! free radical scavenging activity of diclofenac, 10mg/mL concentration

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