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Utilisation of Provitamin A biofortified maize in Ovambo chickens to improve food and nutrition security.

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The skin of female birds had higher Hunter L* (lightness) values ​​than that of males (P<0.05). The interaction of sex and PABM diet affected the copper concentration of Ovambo chicken meat.

  • Introduction to the research problem
  • Importance of the study
  • Overall aim and objectives
  • Hypotheses
  • Study assumptions
  • Study’s limitation
  • Structure of the dissertation
  • References

Determine the effect of provitamin A biofortified maize on the vitamin A concentration of the meat of the female and male Ovambo chickens;. 6. Provitamin A biofortified maize has no effect on the consumer acceptability of meat from the Ovambo chickens.

Vitamin A

Provitamin A carotenoids have the ability to convert to vitamin A when ingested due to the presence of one or more unsubstituted β-ionine rings and an unsaturated hydrocarbon chain that allows them to be converted to vitamin A (Harrison, 2015). Βeta-carotene (Figure 2.1) is the most important provitamin A carotenoid, as it has a higher conversion rate to vitamin A due to the presence of double β-ionine rings that are not present in other provitamin A carotenoids (Harrison, 2015).

Figure 2.2: Conversion of β carotene to retinal  Source: Edem, 2009
Figure 2.2: Conversion of β carotene to retinal Source: Edem, 2009

Importance of vitamin A in humans

  • Immunity
  • Vision
  • Reproduction
  • Growth and development of cells and tissues

Vitamin A in the retinal form is important for good vision and prevention of night blindness (nyctalopia), xerophthalmia, keratomalacia and total blindness (Semba, 2007). The retina in the retina is needed for color vision and vision in low light levels (scotopic vision) (Sherwin et al., 2012).

Vitamin A deficiency

  • Status of VAD in Africa

Vitamin A deficiency in the human body can cause abnormalities in the anterior spinal cord and hindbrain, affecting normal growth and development (Maden, 2007). In South Africa, the deaths of an estimated 3,000 children under four years of age and an estimated 519 maternal deaths were attributed to VAD in the year 2000 (Nojilana et al., 2007).

Challenges of strategies for VAD intervention

  • Dietary supplementation
  • Food fortification
  • Diet diversification
  • Biofortification

The utilization of the nutrients is dependent on the amount of staple food consumed and how it is processed (Bouis et al., 2011). 22 biofortified crops to spread and deliver the seeds to all target areas, however, may take some time (Bouis et al., 2011).

Provitamin A biofortified maize and its potential

For example, it has been estimated that the cost of biofortification of crops over 10 years is about 0.2 % of the cost of global vitamin A supplementation (Mayer et al., 2008). The major carotenoids found in maize are lutein, zeaxanthin, α-carotene, β-carotene and β-cryptoxanthin (Wurtzel et al., 2012).

Retention of β-carotene in PABM after undergoing various processing

24 improves the vitamin A status of consumers and increases the income of producers due to higher productivity.

Consumers’ acceptance of provitamin A biofortified maize

At least a third of the population in Zimbabwe is also said to dislike the taste of PABM. However, they preferred the aroma of the PABM over the other corn lines (Stevens & Winter-Nelson, 2008).

Indigenous chicken production in Africa

Feeding native chickens with PABM should improve the utilization of the provitamin A carotenoids, which can be converted to higher amount of vitamin A in the chicken meat. Possible challenges are the frequency of consumption of chicken meat and eggs, the chicken production management systems, low production and growth rate of the native chickens (King'ori et al., 2010).

Indigenous chicken production and utilization in VAD-prone rural communities

Characteristics such as body weight, gender, plumage color and health status of the native birds are factors that influence their sales price (Moges et al., 2010). It is therefore important to improve the productivity and quality of indigenous chickens for better food security and nutrition.

Impact of vitamin A on growth performance of chickens

Compared to females, males deposit significantly more carotenoids in their when fed a carotenoid-supplemented diet (McGraw et al., 2002). The age, diet and sex of the birds affect feed intake, body weight and daily weight gain in birds (Carrasco et al., 2014).

Influence of vitamin A on metabolism of indigenous chickens

Effects of carotenoids and vitamin A diet in indigenous chickens on meat quality

  • Nutritional composition of meat
  • Sensory evaluation

Various factors such as breed, diet, environment and processing can affect the chemical composition of poultry (Bogosavljevic-Boskovic et al., 2015). The nutritional composition of chicken meat has been successfully manipulated through the diet of chickens (Jensen et al., 1998).

Conclusion of literature review

Therefore, the effect of PABM inclusion in the diet of female and male indigenous chickens should be determined in several stages. The broader objective of the current study was therefore to determine the effect of PABM diet, gender and age of Ovambo chickens on their growth, physiological parameters, meat quality and consumer acceptability.

The Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO). Embryonic development in carotenoid-enriched eggs affects the carotenoid status of the chick after hatching.

Introduction

The increase in consumption demand of indigenous chickens will improve the food security and nutritional status of the resource-poor households raising indigenous chickens. It is therefore important to evaluate whether the available natural sources of vitamin A intake improve the growth performance of the native chickens.

Materials and methods

  • Ethical considerations of the study
  • Study site
  • Birds, treatments and their management
  • Data collection
  • Statistical analyses

The amount of extracted oil was calculated and expressed as a percentage of the original sample. Body weights were obtained by weighing the chicks weekly from day 1 of the experiment (13 weeks old) using a model MEASURETEK OCS-L digital electronic hanging scale (Scale Tronic Services, South Africa).

Results

  • Effect of diet, sex and age on the average daily feed intake of Ovambo chickens
  • Effect of diet, sex and age on the average daily gain of Ovambo chickens
  • Effect of diet, sex and age on the feed conversion ratio of Ovambo chickens

67 3.3.1 Effect of diet, sex and age on the average daily feed intake of Ovambo chickens. There was no significant effect of diet on ADG, although a significant influence of gender on ADG was noted.

Figure 3.1:  Effect of diet, sex and age of bird on ADFI in growing Ovambo chickens
Figure 3.1: Effect of diet, sex and age of bird on ADFI in growing Ovambo chickens

Discussion

The significant interaction of PABM diet and age on ADG agrees with Bhuiyan et al. The higher ADG in male chickens fed the PABM diet compared to male WM chickens agrees with the previous report that supplemental vitamin A significantly increased ADG in supplemented chickens (Kucuk et al., 2003).

Conclusions

75 women up to 20 weeks of age are supported by the findings of Osei-Amponsah et al. The FVV of indigenous breeds fed a standard commercial diet from day 1 to 22 weeks of age in Ethiopia raised under an intensive management system ranged from 11.0 to 18.9 (Hassen et al., 2006), while the current study's VVV ranged from 3.2 to 16.7.

Studies on the growth performance of domestic chicken ecotypes and RIR chickens under an improved management system in northwestern Ethiopia. Effect of age, genotype and gender on growth performance of local chickens reared under improved management in Ghana.

Introduction

Indigenous chickens are one of the most important sources of protein and income for resource-poor households (Mtileni et al., 2009). The aim of the present study was to determine the effect of PABM diet, age and sex of the birds on the carcass and offal of Ovambo chickens.

Materials and methods

  • Study site and the ethical aspect of the study
  • Birds, treatments and their management
  • Data collection
  • Statistical analyses

It was hypothesized that there is no effect of PABM, age and breeding birds or their interactions on the weight of the carcass cuts and offal of Ovambo chickens. The carcass yields were expressed as a ratio of the carcass weight (dressed carcass) and the weight of the internal organs was expressed as a proportion of body weight.

Results

  • Effect of diet, sex and age on the body weight, relative dressed carcass and leg weight
  • Effect of diet, sex and age on the relative breast and back weight of Ovambo chickens
  • Effect of diet, sex and age on the relative gizzard weight of the Ovambo chicken
  • Effect of diet, sex and age on the relative liver weight of Ovambo chickens
  • Effect of diet, sex and age on the relative heart weight of Ovambo chickens

90 Table 4.3: Effect of diet, gender and slaughter age of birds on breast and back weight of Ovambo chickens. 93 4.3.3 Effect of diet, sex and age on the relative weight of the stomach in the Ovambo chicken.

Discussion

Heart weight of PABM-fed male birds at 21 weeks of slaughter was significantly higher than at 18 weeks. The higher curd weight of the female bird is similar to the findings of Zhao et al.

Conclusions

The higher relative weight of the heart in the males can be attributed to their higher growth rate resulting in a higher oxygen demand. The findings in the present study that the relative weight of liver and gizzard was significantly affected by slaughter age, as it decreased with age, is consistent with Tougan et al.

Effects of feeding different levels of cooked and sun-dried fish waste on slaughter characteristics of growing Rhode Island Red chickens. Growth performance, immune response and slaughter characteristics of broilers fed a level of palm kernel cake without or with enzyme supplementation.

Introduction

Indigenous chickens are preferred by consumers due to their distinct organoleptic characteristics and lower fat content (Mtileni et al., 2009; Farrell, 2013). Five countries in southern Africa are among the top 20 countries with VAD in the world (Muthayya et al., 2013).

Materials and methods

  • Study site and the ethical aspects of the study
  • Birds, treatments and management
  • Data collection
  • Statistical analyses

It is necessary to evaluate the interaction between vitamin A-rich diet, age and sex of birds on their blood profiles. The objective of the study was to compare the hematological and biochemical responses of female and male Ovambo chickens to PABM at different ages.

Results

  • Effect of diet, sex and age on haematological parameters of the Ovambo chicken
  • Effect of diet, sex and age on serum biochemistry of Ovambo chickens

The PABM fed male birds at 21 weeks of age had a significantly higher ALB than the WM fed male of the same age. The female birds at 21 weeks of age had significantly higher globulin than the male birds of the same age.

Discussion

Total protein in female birds at both slaughter ages was higher than male birds. The high level of creatinine in female chicks at 21 weeks is due to hormonal and metabolic changes as a result of sexual maturity (Menon et al., 2013).

Conclusions

Serum biochemical constituents are positively correlated with the quality of the diet (Adeyemi et al., 2000; Etuk et al., 2014).

An evaluation of the effect of Moringa Olifera leaf powder as a dietary nutritional supplement. Hematological, electrolyte and serum biochemical values ​​of native Thai chickens (Gallus domesticus) in northeastern Thailand.

Introduction

There is therefore a need to evaluate the effect of PABM diet, sex and age on the meat quality of the Ovambo chicken. It was assumed that PABM, the sex and age of the birds have no influence on the meat quality of the Ovambo chickens.

Materials and methods

  • Study site and the ethical aspect of the study
  • Birds, treatments and management
  • Slaughtering of birds
  • Meat quality measurements
  • Statistical analyses

Other factors that affect meat quality are the breed, age and gender of the chicken. The aim of the study was to determine the effect of maize diet meat enriched with provitamin A on the quality of male and female Ovambo chickens at different ages.

Results

  • Effect of diet, sex and age on the pH of the breast muscle of Ovambo chickens
  • Effect of diet, sex and age on the drip loss of the breast muscle of Ovambo chickens
  • Effect of diet, sex and age on meat colour of the breast muscle of Ovambo chickens

PABM-fed female birds at 21 weeks of age had a higher L* value than age-matched PABM-fed male birds. PABM-fed male birds aged 21 weeks at slaughter have a higher a* value than WM-fed male birds of the same age (Table 6.5).

Discussion

The findings of the present study on the effect of PABM on the a* value of breast meat and skin are consistent with the findings of Rajput et al. The result in the present study on the effect of age and gender on a* value on meat color is consistent with Dadgar et al.

Conclusions

A high L* value in the skin and meat quality is an indication of PSE meat, which reduces the quality of the meat. Flesh color is influenced by several factors, including the age and diet of the birds (Lyon & Lyon, 2001).

Effect of supplementation of α-tocopherol and β-carotene on meat quality and antioxidant capacity in pigs fed a high linoleic diet. Nutrient density and slaughter age have different effects on carcass performance, muscle and meat quality in fast- and slow-growing broiler genotypes.

Introduction

It is possible to improve the nutritional value of chicken meat by manipulating the diet of the birds (Owens et al., 2010). It was hypothesized that PABM diet and sex have no effect on the nutritional value of the Ovambo meat.

Materials and methods

  • Study site and the ethical aspects of the study
  • Birds, treatments and management
  • Slaughter of the birds
  • Chemical and mineral composition of the breast meat samples
  • Statistical analyses

The beakers were clamped in the Soxhlet machine and the electric heating plates were raised to the base of the beakers. To determine the crude protein, the total nitrogen content was determined by Kjeldahl nitrogen analysis, according to AOAC (1995).

Results

  • Effect of diet and sex of Ovambo chicken on the proximate composition of breast meat
  • Effect of diet and sex of Ovambo chicken on the mineral composition of breast meat

157 Table 7.3: Effects of bird diet and gender on the chemical composition of Ovambo chicken breast meat. 158 Table 7.4: Effect of bird diet and gender on the mineral composition of Ovambo chicken breast meat.

Discussion

The lack of sex effect on the protein level in breast meat agrees with De Marchi et al. 2005) and Intarapichet and Maikhunthod (2005) on indigenous chickens. The higher ash content in the breast meat of female birds than male birds is similar to the present study.

Conclusions

163 The similarity in mineral composition of breast meat between males and females agrees with Geldenhuys et al. 2015) on geese, except that there was an influence of gender on copper content in the current study. This may be because breast meat has a low amount of myoglobin, which contains iron, and a high amount of white fibers (Lengerken et al., 2002).

Breed, sex, diet, management system and type of meat affect the chemical and nutritional composition of chicken meat (Bogosavljevic-Boskovic et al., 2010). Effect of dietary protein and energy level on the proximate composition of breast and thigh meat in white leghorn layers during the molt and post-molt production stages.

  • Introduction
  • Materials and methods
    • Birds, treatments and their management
    • Ethical aspects of the study and study site
    • Slaughter of birds
    • Meat sample preparation
    • Data collection
  • Results
    • Effect of diet, age and gender of consumers on taste of Ovambo chicken meat
    • Effect of diet, gender and age of consumers on the texture of meat
    • Effect of diet, gender and age of consumers on the colour of Ovambo chicken
    • Effect of diet, gender and age of consumers on the aroma of Ovambo chicken meat 181
  • Discussion
  • Conclusions
  • References

If PABM improves the quality of Ovambo meat but negatively affects its sensory properties, the meat acceptability will be low. The thigh and drumstick samples were cooked in the traditional way of the Mhkambathini community.

General discussion

Conclusions

Recommendations

Animal Research Ethics Approval

Humanities & Social Sciences Research Ethics Approval

Approval from Mkhambathini Municipality

Consent Form for Consumer’s Acceptability in English

Consent Form for Consumer’s Acceptability in Isizulu

Paired Preference Test in English

Paired Preference Test in Isizulu

Sensory Evaluation Questionnaire in English

Sensory Evaluation Questionnaire in Isizulu

Publications

Gambar

Figure 2.2: Conversion of β carotene to retinal  Source: Edem, 2009
Figure 3.1:  Effect of diet, sex and age of bird on ADFI in growing Ovambo chickens
Figure 3.2: Effect of diet, sex and age of bird on the ADG of Ovambo chickens
Figure 3.3: Effect of diet, sex and age of bird on the mean of FCR of the Ovambo chicken   WM: white maize, PABM: provitamin A biofortified maize, FCR: feed conversion ratio

Referensi

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