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Fig. 1. (A) Construction of the competitor template, pCC-Compet. Only the region between the two primers, 1579 and2771, used in the cccDNA PCR assay is shown
Fig. 2. (A) Quantitative PCR of DHBV cccDNA. 22.2 ng of duck liver DNA (8.4×restriction endonuclease and separated and visualized on an agarose gel
Fig. 3. (A) Quantitation of DHBV cccDNA by Southern blot. A series of reactions were assembled, each contained 0.8 �g of duckliver DNA (3.0×105 diploid genomes)
Fig. 4. Range of the cccDNA PCR assay. Quantitative PCRassays were performed using the same duck liver DNA prepa-ration but varying the amounts of DNA/PCR reaction overthe range 2.5–60 ng

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