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Fig. 1. Biosynthesis of isoflavan phytoalexins, vestitol and sativan. P450 enzymes involved are emphasized in bold letters
Fig. 2. HPLC profile of exudates of LAfter 10 h elicitation with 10 mM GSH, an isoflavan vestitol,which was not detected in the control (H
Fig. 3. Cloning strategy of P450 cDNAs by PCR. Six degen-erate oligonucleotide primers were designed from conservedregions of P450s of the flavonoid pathway.
Fig. 4. Amino acid sequence alignments of LjCYP-1 and LjCYP-2. Gaps (-) are inserted to optimize the alignments
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