BAB V. KESIMPULAN DAN SARAN
B. Saran…
Perlu dilakukan penelitian lebih lanjut mengenai hubungan SNPs gen PRKAA2 rs857148 terhadap penyakit metabolik serta dilakukan sekuensing untuk memastikan perubahan rangkaian asam amino ketika terjadi SNPs di daerah 3’UTR yang berpengaruh terhadap fungsinya dalam stabilisasi dan translasi mRNA.
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LAMPIRAN
Lampiran 1. Ethical Clearance Penelitian
Lampiran 2. Go Taq Green Master Mix Certificate of Analysis
Lampiran 3. Usage Information of Go Taq Green Master Mix
Lampiran 4. Product Datasheet of DNA Ladders and Markers
Lampiran 5. Informasi Primer Gen PRKAA2 rs857148
Lampiran 6. Informasi Enzim Restriksi BsuRI
Lampiran 7. Hasil Analisis Kualitatif Isolat DNA
Lampiran 8. Hasil Optimasi Suhu Annealing Gen PRKAA2 rs857148
Lampiran 9. Hasil Optimasi Konsentrasi Primer Gen PRKAA2 rs857148
Lampiran 10. Hasil Validasi Metode PCR
Lampiran 11. Hasil Pemotongan Produk PCR Menggunakan Enzim BsuRI
Lampiran 12. Perhitungan Konsentrasi Primer
Konsentrasi stok primer yaitu 100 μM, untuk mendapatkan konsentrasi ini maka dilarutkan dengan 250 μL. Dibuat larutan intermediet dengan melakukan pengenceran 2x dalam 10 μL. Konsentrasi 50 μM sebagai konsentrasi larutan intermediet.Volume yang diambil untuk optimasi konsentrasi primer, yaitu 1; 1,25;
1,5; dan 1,75 μL dalam 25 μL sehingga konsentrasi yang didapatkan:
C1.V1 = C2.V2
50 μM.1 μL = C2.25 μL C2 = 2 μM
C1.V1 = C2.V2
50 μM.1,5 μL = C2.25 μL C2 = 3 μM
C1.V1 = C2.V2 C1.V1 = C2.V2
50 μM.1,25 μL = C2.25 μL C2 = 2,5 μM
50 μM.1,75 μL = C2.25 μL C2 = 3,5 μM
Lampiran 13. Perhitungan Konsentrasi Buffer TBE 1x
Konsentrasi buffer stok adalah 10x. TBE dibuat dengan mengambil 50 mL buffer TBE 10x lalu ditambahkan WFI hingga 500 mL.
C1.V1 = C2.V2
10x.50 mL = C2.500 mL C2 = 1x
Lampiran 14. Analisis Spesifisitas Primer Gen PRKAA2 rs857148