BAHAN DAN METODE
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Perbesaran Lokasi A di P. Madura
Lampiran 1. Peta Lokasi Pengambilan sampel sapi madura di Pulau Madura
Perbesaran Lokasi B di P. Madura
Keterangan: P. JAWA P. BALI
A B
P. MADURA Komis Tadan Tengah Polagan Golbung Patereman Anjun Serabi Timur Paterongan 3 7 43 49 54 51 52 38 40 41 13 14 26 29 30 32Angka pada peta menunjukkan nomor koleksi sampel darah sapi madura
U
B T
S
34
Lampiran 2 Hasil penjajaran DNA ruas Cyt b,tRNA Thr, tRNA Pro dan Dloop
genom mitokondria sapi madura dengan data GenBank. Keterangan:
nomor posisi nukleotida dibaca secara vertikal pada tiga baris
pertama berdasarkan referensi B. indicus (AF492350).
Ruas Cyt 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 0000000000 0001111111 1111111111 1111111111 11111111 8889999999 9990000000 0001111111 1112222222 22233333 7890123456 7890123456 7890123456 7890123456 78901234 Bubalusbub GAAGTATGAT GTTCCGGCCA TTCAGCCAAT GCCTATTCTG AATTCTAG
BosTaurus1 ....C..A.. A...A... C... ... .GCC....
BosIndicus ....C..A.. A...A... C... ... .GCC....
BosTaurus_BC ....C..A.. A...A... C... ... .GCC....
BosTaurus_FH ....C..A.. A...A... C... ... .GCC....
BosJavanic1 ....C..A.. A...A... C... ...T.. ..C...
BosJavanic2 ....C..A.. A...A... C... ...T.. ..C...
Madura_14 ....C..A.. A...A... C... ... .GCC....
Madura_26 ....C..A.. A...A... C... ... .GCC....
Madura_38 ....C..A.. A...A... C... ... .GCC....
Madura_32 ....C..A.. A...A... C.A... ...T.. ....T..A Madura_41 ....C..A.. A...A... C.A... ...T.. ....T..A Madura_29 ....C..A.. A...A... C.A... ...T.. ....T..A 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 1111111111 1111111111 1111111111 1111111111 11111111 3333344444 4444455555 5555566666 6666677777 77777888 5678901234 5678901234 5678901234 5678901234 56789012 Bubalusbub TAGCAAACCT GCTAACACTC ACATGGATTG GAGGACAGCC AGTCGAAC BosTaurus1 ...G.... A..G... ...A.... ...A.. ...
BosIndicus ...G.... A..G... ...A.... ...A.. ...
BosTaurus_BC ...G.... A..G... ...A.... ...A.. ...
BosTaurus_FH ...G.... A..G... ...A.... ...A.. ...
BosJavanic ...G.T.. AT... ...A.... ...A.. ...
BosJavanic ...G.T.. AT... ...A.... ...A.. ...
Madura_14 ...G.... A..G... ...A.... ...A.. ...
Madura_26 ...G.... A..G... ...A.... ...A.. ...
Madura_38 ...G.... A..G... ...A.... ...A.. ...
Madura_32 ...G.T.. A... ...A..C. ... ...
Madura_41 ...G.T.. A... ...A..C. ... ...
35 Lampiran 2 (Lanjutan) 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 1111111111 1111111222 2222222222 2222222222 22222222 8888888999 9999999000 0000000111 1111111222 22222223 3456789012 3456789012 3456789012 3456789012 34567890 Bubalusbub ACCCATATAT TATCATTGGA CAACTAGCAT CTATCACATA CTTCCTCC BosTaurus1 ... C.C...C... ... ..G..CT... ...T.... BosIndicus ... C.C... ... ...CT... ...T..T. BosTaurus_BC ... C.C...C... ... ..G..CT... ...T.... BosTaurus_FH ... C.C...C... ... ..G..CT... ...T.... BosJavanic1 ... ..C... ...T... .C....T... ...T..T. BosJavanic2 ... ..C... ...T... .C....T... ...T..T. Madura_14 ... C.C... ... ...CT... ...T..T. Madura_26 ... C.C... ... ...CT... ...T..T. Madura_38 ... C.C... ... ...CT... ...T..T. Madura_32 ... ..C... ... .C..T.T... ...T..T. Madura_41 ... ..C... ... .C..T.T... ...T..T. Madura_29 ... ..C... ... .C..T.T... ...T..T. 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 2222222222 2222222222 2222222222 2222222222 22222222 3333333334 4444444445 5555555556 6666666667 77777777 1234567890 1234567890 1234567890 1234567890 12345678 Bubalusbub TCATCCTAGT GCTAATACCA ACGGCCAGCA TAATCGAAAA TAATCTCT BosTaurus1 ... ... ...G... C... C..AT.AC BosIndicus ... A... ..A...G... C.G.T... C..AT.AC BosTaurus_BC ... ... ...G... C... C..AT.AC BosTaurus_FH ... ... ...G... C... C..AT.AC BosJavanic1 ... A... ..A...G... C.G... C..C...C BosJavanic2 ... A... ..A...G... C.G... C..C...C Madura_14 ... A... ..A...G... C.G.T... C..AT.AC Madura_26 ... A... ..A...G... C.G.T... C..AT.AC Madura_38 ... A... ..A...G... C.G.T... C..AT.AC Madura_32 ... A... ..A...G... C.G... ...A..AC Madura_41 ... A... ..A...G... C.G... ...A..AC Madura_29 ... A... ..A...G... C.G... ...A..A.
36 Lampiran 2 (Lanjutan) 11111111111 55555555555 22222222222 78888888888 90123456789 Bubalusbub TAAAATGAAGA BosTaurus1 ... BosIndicus ... BosTaurus_BC ... BosTaurus_FH ... BosJavanic1 ... BosJavanic2 ... Madura_14 ... Madura_26 ... Madura_38 ... Madura_32 ... Madura_41 ... Madura_29 ...
37 Lampiran 2 (Lanjutan) Ruas Dloop 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 4444444444 4444444444 4444444444 4444444444 44444444 3333333333 4444444444 5555555555 6666666666 77777777 0123456789 0123456789 0123456789 0123456789 01234567 B.javanicus3 ATTTGAACTA TTTCCTAAGC ACTATTAATA TAGTTCCATA AACGCAAA
Madura 29 ... ... ... ... ...
Madura 41 ... ... ... ... ...
Madura 32 ... ... ... ... ...
B. taurus --- ---.A. ... ... ..TA....
B. indicus --- ---.A. ... ... ..T...
Madura 14 --- ---.A. ... ... ..T...
Madura 26 --- ---.A. ... ... ..T...
Madura 38 --- ---.A. ... ... ..T...
Bub bubalus --- ---.AT ... ...C....C. ..T...
1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 4444444444 4444444444 4444444444 4444444444 44444444 3333333333 3333333333 3333333333 3333333333 33333333 8901234567 8901234567 8901234567 8901234567 89012345 B.javanicus3 GAGCCTTGCC AGTATTAAAT TTACCAAAAA TTTCAACAGC TCAACACA Madura 29 ... ... ... ...T... ...
Madura 41 ... ... ... ... ...
Madura 32 ... ... ... ... ...
B. taurus ...AT. ... ...T... .CC...T.A. ...
B. indicus ...AT. ... ...T... .CC...T.A. ...
Madura 14 ...AT. ... ...T... .CC...T.A. ...
Madura 26 ...AT. ... ...T... .CC...T.A. ...
Madura 38 ...AT. ... ...T... .CC...T.A. ... Bub bubalus ...CT. ...C.... .C..T....C ..G...A. .T...T
38 Lampiran 2 (Lanjutan) 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 4444444444 4444444444 4444444444 4444444444 44444444 3333444444 4444555555 5555666666 6666777777 77778888 6789012345 6789012345 6789012345 6789012345 67890123 B.javanicus3 GAATTTGTAC CCTAACCAAA TATCACAAAC ACCACTAGCT AATAACAC
Madura 29 ... ... ... ... ...
Madura 41 ... ... ... ... ...
Madura 32 ... ... ... ... ...
B. taurus ...AC.. ... ...T... ... ..C.TA..
B. indicus ...C.. ... ...T... ... ..C.TA..
Madura 14 ...C.. ... ...T... ... ..C.TA..
Madura 26 ...C.. ... ...T... ... ..C.TA..
Madura 38 ...C.. ... ...T... ... ..C.TA..
Bub bubalus ..C...AC.. T...G..T.. C..T.G...T .A.TAC.A.C .TC...
1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 4444444444 4444445555 5555555555 5555555555 55555555 8888889999 9999990000 0000001111 1111112222 22222233 4567890123 4567890123 4567890123 4567890123 45678901 B.javanicus3 ACGCTCCCCA CTACAGAATG CACCCTCCAA GTGGGATAA- ---
Madura 29 ....C... ... ... ...- ---
Madura 41 ... ... ... ...- ---
Madura 32 ....C... ... ... ...- --- B. taurus ..----G..C A....C.GAC ...AGAATG. A.TA-CCT.- ---CGC B. indicus ..----G..C A....C.GAC ...AGAATG. A.TA-CCC.- ---GGC Madura 14 ..----G..C A....C.GAC ...AGAATG. A.TA-CCC.- ---GGC Madura 26 ..----G..C A....C.GAC ...AGAATG. A.TA-CCC.- ---GGC Madura 38 ..----G..C A....C.GAC ...AGAATG. A.TACCCC.- ---GGC Bub bubalus ..CTGA..TC A..TGT.CAA ...A.AA..T A..ACCCT.C TACTCCGA
39 lampiran 2 (Lanjutan) 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 5555555555 5555555555 5555555555 5555555555 55555555 3333333344 4444444455 5555555566 6666666677 77777777 2345678901 2345678901 2345678901 2345678901 23456789 B. javanicus --- GTACATAATA TTAATGTAAT AAGTACATAA TATTAATG
Madura 29 --- ... ... ... ... Madura 41 --- ... ... ... ... Madura 32 --- ... ... ... ... B. taurus AAGGGGTAAT ...C. ... ..--- --- B. indicus AAGAGGTAAT ...C. ... ..--- --- Madura 14 AAGAGGTAAT ...C. ... ..--- --- Madura 26 AAGAGGTAAT ...C. ... ..--- --- Madura 38 AAGAGGTAAT ...C. ... ..--- ---
Bub bubalus ATGGGGGGGG .G...C. ... ..--- ---
1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 5555555555 5555555555 6666666666 6666666666 66666666 8888888888 9999999999 0000000000 1111111111 22222222 0123456789 0123456789 0123456789 0123456789 01234567 B. javanicus3 TAATAAGTAC ATAATATTAA TGTAATAAGT ACATAATATT AATGTAAT Madura 29 ... ... ... ... ... Madura 41 ... ... ... ... ... Madura 32 ... ... ...-- --- --- B. taurus --- --- --- --- --- B. indicus --- --- --- --- --- Madura 14 --- --- --- --- --- Madura 26 --- --- --- --- --- Madura 38 --- --- --- --- --- Bub bubalus --- --- --- --- ---
40 Lampiran 2 (Lanjutan) 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 6666666666 6666666666 6666666666 6666666666 66666666 2233333333 3344444444 4455555555 5566666666 66666666 8901234567 8901234567 8901234567 8901234567 89012345 B. javanicus3 AGTACATAAT ATTAATGTAA TAAGTACATA ATATTAATGT AATAAGTA
Madura 29 ... ... ... ... ... Madura 41 ... ... ... ... ... Madura 32 --- --- --- --- --- B. taurus --- --- --- --- --- B. indicus --- --- --- --- --- Madura 14 --- --- --- --- --- Madura 26 --- --- --- --- --- Madura 38 --- --- --- --- --- Bub bubalus --- --- --- --- --- 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 6666666666 6666666666 6666777777 7777777777 77777777 7777888888 8888999999 9999000000 0000111111 11112222 6789012345 6789012345 6789012345 6789012345 67890123 B. javanicus3 CATAATATTA ATGTAATAAG TACATAATAT TAATGTAATA AGTACATA Madura 29 ... ... ... ... ... Madura 41 ... ... ... ... ... Madura 32 --- --- --- --- --- B. taurus --- --- --- --- --- B. indicus --- --- --- --- --- Madura 14 --- --- --- --- --- Madura 26 --- --- --- --- --- Madura 38 --- --- --- --- --- Bub bubalus --- --- --- --- ---
41 Lampiran 2 (Lanjutan) 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 7777777777 7777777777 7777777777 7777777777 77777777 2222223333 3333334444 4444445555 5555556666 66666677 4567890123 4567890123 4567890123 4567890123 45678901 B. javanicus3 ATATTAATGT AATAAAGACA TAATATGTAT ATAGTACATT ACATTACA
Madura 29 ... ... ... ... ... Madura 41 ... ... ... ... ... Madura 32 --- ---... ... ... ... B. taurus --- ---... ... ... .A....T. B. indicus --- ---... .G... ... .A....T. Madura 14 --- ---... .G... ... .A....T. Madura 26 --- ---... .G... ... .A....T. Madura 38 --- ---... .G... ... .A....T. Bub bubalus --- ---G.... ... ... .T....T. 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 7777777777 7777777777 7777777788 8888888888 88888888 7777777788 8888888899 9999999900 0000000011 11111111 2345678901 2345678901 2345678901 2345678901 23456789 B. javanicus3 TACCCCATGC ATATAAGCAA GTACTTGAAC -TCCATAGAC AGTACATA Madura 29 ... ... ... -... ... Madura 41 ... ... ... -... ... Madura 32 ... ... ... -... ... B. taurus .G... ... ....A...C. -..T...-G. ... B. indicus ... ... ....A...T. -..T...-.T ... Madura 14 ... ... ....A...T. -..T...-.T ... Madura 26 ... ... ....A...C. -..T...-.T ... Madura 38 ... ... ....A...T. -..T...-.T ...
42 Lampiran 2 (Lanjutan) 1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 8888888888 8888888888 8888888888 8888888888 88888888 2222222222 3333333333 4444444444 5555555555 66666666 0123456789 0123456789 0123456789 0123456789 01234567 B. javanicus3 GTACATTAAT CCACTAATCG TACATAGTAC ATT-ACATCA AATCTACC Madura 2 ... ... ...C.. ...-.T.... ...T. Madura 41 ... ... ... ...-... ... Madura 32 ... ... ... ...-... ... B. taurus A...AT.A TT.T.G.CT. ... ...-.TG... ...TC.TT B. indicus A...AC.A TT.T...C.. ... ...-.T.... ....C.T. Madura 14 A...AC.A TT.T....T. ... ...-.T.... ....C.T. Madura 26 A...AC.A TT.T....T. ... ...-.T.... ....C.T. Madura 38 A...AC.A TT.T....T. ... ...-.T.... ....C.T. Bub bubalus C.A TT.T.G.... ...G. ...C.AG... ....CGT.
1111111111 1111111111 1111111111 1111111111 11111111 5555555555 5555555555 5555555555 5555555555 55555555 8888888888 8888888888 8888888888 8899999999 99999999 6677777777 7788888888 8899999999 9900000000 00000000 8901234567 8901234567 8901234567 8901234567 89012345 B. javanicus3 CTTGACAACA TGC--- ATATCCCTT- CCACTAGATC ACGAGATT Madura 29 ... ...--- ...- ... ... Madura 41 ... ...--- ...- ... ... Madura 32 ... ...--- ...- ... ... B. taurus ...T.GT. .ATCTATTAT ....T....A ...T... ...C.. B. indicus ..CA... .ATCTACTAT ...C...C.- ... ...C.. Madura 14 ..CA... .ATCTACTAT ...C...- ... ...C.. Madura 26 ..CA... .ATCTACTAT ...C...- ... ...C.. Madura 38 ..CA... .ATCTACTAT ...C...- ... ...C.. Bub bubalus ..C.C... ...--- ...C.- ... ...C..
43 Lampiran 2 (Lanjutan) 11111 55555 99999 00000 67890 B. javanicus3 AACCA Madura 29 ... Madura 41 ... Madura 32 ... B. taurus ..TT. B. indicus ..TT. Madura 14 ..TT. Madura 26 ..TT. Madura 38 ..TT. Bub bubalus GGT..