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Lampiran 1 Pengaruh penambahan HCl 0,1 N terhadap perubahan pH
Lampiran 2 Hasil uji kelarutan melanin
Pelarut Hasil
Asam klorida Tidak terlarut
24 Pelarut Hasil Natrium hidroksida (NaOH) Larut
Akuades Tidak larut
Lampiran 3 Hasil fitokimia
Flavonoid
Lapisan berwarna jingga (+)
Tanin Warna hijau tua (-)
Saponin Busa yang stabil (-)
Alkaloid Terbentuk endapan (-)
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Lampiran 4 Pengendapan melanin dengan berbagai pelarut
No Jenis larutan Gambar
1 Akuades
2 Etil asetat
3 n-heksana
4 Kloroform
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No. Jenis pelarut Gambar
6 Asam klorida (HCl)
Lampiran 5 Bobot miselia pertumbuhan kapang RS3 Hari ke Biomasa 0 0,0635 3 0,089 6 0,161 9 0,177 12 0,202 15 0,333 18 0,342 21 0,507 24 0,478 27 0,418 Sumber: Sahara 2013
Lampiran 6 Analisis ragam pengaruh pH terhadap pengendapan
pH 4 3,5 3 2,5 2
Ulangan 1 0 0 0 0,15 0,14
Ulangan 2 0 0 0 0,17 0,12
SK JK dB KT Fhit P-value Ftabel
Between Groups 0,05136 4 0,01284 160,5 1,83x10-5 5,192168 Within Groups 0,0004 5 8x10-5
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Uji lanjut DMRT (Duncan’s Multiple Range Test)
Nilai pH N Rata-rata Notasi
4,0 2 0,00 A 3,5 2 0,00 A 3,0 2 0,00 A 2,5 2 0,16 C 2,0 2 0,13 B Histogram 0 0 0 0,16 0,13 0 0,02 0,04 0,06 0,08 0,1 0,12 0,14 0,16 0,18 4 3,5 3 2,5 2 Re nde me n pH a a a c b
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