4 HASIL DAN PEMBAHASAN
DAFTAR PUSTAKA
Transformasi genetik padi Indica cv. Kasalath dengan gen penyandi superoksida dismutase (MmCu/Zn-SOD) dari Melastoma malabatricum
menghasilkan 2 tanaman transgenik. Analisis pewarisan gen hpt ada generasi T1 dan generasi T2 dari padi Indica cv. Kasalath transgenik menunjukkan bahwa gen
hpt diwariskan secara stabil kepada generasi selanjutnya, mengikuti hukum Mendel dengan satu salinan gen yang terintegrasi ke dalam genom. Gen
MmCu/Zn-SOD terpaut dengan gen hpt, maka inetgrasi dan pewarisannya juga seperti pada gen hpt.
Saran
Efisiensi regenerasi dapat ditingkatkan dengan menggunakan media regenerasi tanpa penambahan antibiotik. Fungsi gen MmCu/Zn-SOD terhadap ketahanan cekaman abiotik dapat dianalisis dengan uji tantang pada galur tanaman transgenik SOD dengan menggunakan transgenik homozigot.
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Lampiran 1 Komposisi media AB plate
Komposisi Bahan Konsentrasi mg/L
Bacto agar 15000 Glukosa 5000 Salt NH4Cl KCL MgSO4.7H2O CaCl2.2H2O FeSO4.7H2O 1000 50 300 13.25 2.5 Buffer NaH4PO4 KH2PO4 1000 3000
21 Lampiran 2 Komposisi media dasar N6
Komposisi Bahan Konsentrasi mg/L
N6 Minor Salt MnSO4.4H2O ZnSO4.7H2O H3BO3 KI 4.4 1.5 1.6 8.0 N6 Major Salt KNO3 (NH4)2SO4 CaCl2.2H2O KH2PO4 MgSO4.7H2O 283 463 166 400 185 N6 Vitamin Gly Nicotinic acid Pyridoxine hydrochloride Thiamine hydrochloride 2.0 0.5 0.5 1.0 FeEDTA FeSO4.7H2O Na2EDTA 27.8 37.3
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Lampiran 3 Komposisi media 2NBKCH50
Komposisi Bahan Konsentrasi mg/L
N6K Major Salt KNO3 CaCl2.2H2O MgSO4.7H2O KH2PO4 2022 166 185 40 B5 Minor Salt MnSO4.4H2O ZnSO4.7H2O CuSO4.5H2O Na2MoO4.2H2O CoCl2.6H2O H3BO3 KI 13.2 2 0.025 0.25 0.025 3 0.75 B5 Vitamin Myo-inositol Thiamine hyfrochloride Pyridoxine hyfrochloride Nicotinic acid 100 10 1 1 AA Amino Acid Glutamine Aspartic acid Arginin Glycin 876 266 174 0075 FeEDTA FeSO4.7H2O Na2EDTA 27.8 37.3 Maltosa Higromisin Cefotaximee Gelrite 30000 50 200 4000
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RIWAYAT HIDUP
Penulis dilahirkan di Rupat, Riau pada tanggal 4 Oktober 1988 dari pasangan bapak Zahidin SHI dan ibu Gusniwarti S.Pd.SD. Penulis merupakan anak pertama dari lima bersaudara.
Penulis menyelesaikan pendidikan menengah di SMA Negeri 1 Bengkalis, Riau pada tahun 2006. Pada tahun yang sama penulis, melanjutkan pendidikan sarjana di Jurusan Biologi FMPIA, Universitas Riau melalui jalur Seleksi Penerimaan Mahasiswa Baru (SPMB). Tahun 2012 penulis menyelesaikan pendidikan sarjana Biologi. Pada tahun yang sama penulis melanjutkan pendidikan di Program Studi Biologi Tumbuhan, Sekolah Pascasarjana Institut Pertanian Bogor dengan sponsor dari program Beasiswa Unggulan Dikti (BU DIKTI) tahun 2012.
Selama menjalani pendidikan S2, penulis aktif menjadi asisten praktikum Rekayasa Genetika tahun ajaran 2013/2014. Penulis juga aktif di berbagai organisasi mahasiswa Pascasarjana IPB, yaitu sebagai Bendahara Umum Forum Mahasiswa Pascasarjana IPB, anggota Departemen Keilmuan Himpunan Mahasiswa Muslim Pascasarja IPB. Penulis merupakan salah satu perintis berdirinya Kelompok Keilmuan Pascasarjana IPB, Bogor Science Club (BSC) pada tahun 2013. Selain itu penulis aktif mengikuti kegiatan ilmiah seperti seminar, workshop dan pelatihan, baik lingkup nasional maupun internasional yang diselenggarakan instansi terkait di Indonesia.