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LAMPIRAN
Isolasi bakteri endofit
Isolat bakteri endofit
Uji aktivitas antibakteri
Aktivitas antibakteri (zona bening)
Isolasi DNA genom
DNA genom
Amplifikasi menggunakan mesin PCR
Kekerabatan isolat Sekuensing DNA
Urutan basa DNA
Identifikasi menggunakan website NCBI Lampiran 1 Bagan alir penelitian
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Lampiran 2 Morfologi koloni isolate bakteri endofit
Ket: C ( Cianjur), G (Garut), S (Sukabumi), 1,2,3 (ulangan) *interaksi ditandai dengan terbentuknya zona hambat Kode
isolat
Warna Koloni Tepian Elevasi Interaksi* C1.3 Putih susu Berlendir Rata Cembung
C2.2 Kuning Berlendir Rata Rata v
C2.3 Putih susu Berlendir Rata Cembung v
C4.1 Kuning Berlendir Rata Cembung v
C4.2 Orange Berlendir Rata Cembung v
C4.3 Putih susu Berlendir Rata Cembung v
C5.1 Kuning Berlendir Rata Rata v
C5.2 Putih kekuningan
Tidak berlendir
Bergerigi Rata
G1.2 Kuning Berlendir Rata Cembung
G2.1 Putih susu Berlendir Rata Rata
G2.2 Putih susu Berlendir Rata Rata v
G2.4 Kuning Berlendir Rata Cembung
G3.3 Kuning Berlendir Rata Cembung v
G3.4 Kuning Berlendir Rata Cembung
G3.5 Kuning Berlendir Rata Cembung
G4.2 Kuning Berlendir Rata Cembung v
G4.5 Kuning Berlendir Rata Cembung v
G5.1 Orange Berlendir Rata Cembung v
G5.2 Kuning Berlendir Rata Cembung
G5.3 Kuning Berlendir Rata Cembung v
G5.4 Orange Berlendir Rata Cembung v
S1.3 Kuning Berlendir Rata Cembung
S1.4 Putih susu Tidak berlendir
Bergelombang Cembung S4.1 Putih susu Tidak
berlendir
Bergelombang Cembung v S4.2 Putih susu Tidak
berlendir
Bergelombang Cembung v
S4.3 Putih susu Berlendir Rata Rata v
S4.5 Putih susu Berlendir Rata Rata v
S5.1 Putih susu Berlendir Rata Rata
S5.4 Putih susu Berlendir Rata Rata v
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Lampiran 3 Isolar bakteri endofit dari tiga daerah
Gambar isolat bakteri endofit asal Cianjur
Gambar isolat bakteri endofit asal Garut
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Lampiran 4 Komposisi larutan yang digunakan Kalium Asetat
TAE (Tris-Asetat-EDTA) 50x
Buffer ekstrak
Kappa 2G Fast ReadyMix yang diproduksi oleh KAPABIOSYSTEM
3 M Kalium 5 M Asetat pH 4.8 242 g basa Tris 57.1 mL Asetat glasial 100 mL 0.5 M EDTA Ph 8 100 mM Tris HCl 10 mL 100 mM EDTA 20 mL 250 mM NaCl 5 mL 1 % SDS 5 mL 1 % PVP 1 g
DNA polimerase 0.5 U per 25 µL reaksi
Buffer PCR dNTPs 0.2 mM
MgCl2 1 mM dalam 1x Stabilizers
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23
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Lampiran 7 Sekuen DNA isolat G42
GCCTTGAATGCAGTCCTATGCCCGCCGTGTTAANGAAGAAAGGCCCTTTCG GGATTTGTAAAAGTACCTTTCAAGCGGGGAAGGAAAGGCGGGTGAGGGTTAAATA ACCCTTGGCCGATTTGACCGTTACCCGGCAGAAGGAAGCACCGGGCTAACTTCCG GGGCCAGCAAGCCGCGGTAATTCGGAAGGGTGCAAAGCGTTAATTCGGAATTAAC TGGGCGTAAAGGCGCACGCAGGGCGGTTCTGTTAAGTCCAGATGTGAAATCCCCC GGGCTTAACCTGGGAACTGCATTTGAAACTGGCAGGCTTGAGTCTCGTAGAGGGG GGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTG GCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGC AAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGT TGTGCCCTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGA GTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTG GAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGCCTTGA
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