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LAMPIRAN

Isolasi bakteri endofit

Isolat bakteri endofit

Uji aktivitas antibakteri

Aktivitas antibakteri (zona bening)

Isolasi DNA genom

DNA genom

Amplifikasi menggunakan mesin PCR

Kekerabatan isolat Sekuensing DNA

Urutan basa DNA

Identifikasi menggunakan website NCBI Lampiran 1 Bagan alir penelitian

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Lampiran 2 Morfologi koloni isolate bakteri endofit

Ket: C ( Cianjur), G (Garut), S (Sukabumi), 1,2,3 (ulangan) *interaksi ditandai dengan terbentuknya zona hambat Kode

isolat

Warna Koloni Tepian Elevasi Interaksi* C1.3 Putih susu Berlendir Rata Cembung

C2.2 Kuning Berlendir Rata Rata v

C2.3 Putih susu Berlendir Rata Cembung v

C4.1 Kuning Berlendir Rata Cembung v

C4.2 Orange Berlendir Rata Cembung v

C4.3 Putih susu Berlendir Rata Cembung v

C5.1 Kuning Berlendir Rata Rata v

C5.2 Putih kekuningan

Tidak berlendir

Bergerigi Rata

G1.2 Kuning Berlendir Rata Cembung

G2.1 Putih susu Berlendir Rata Rata

G2.2 Putih susu Berlendir Rata Rata v

G2.4 Kuning Berlendir Rata Cembung

G3.3 Kuning Berlendir Rata Cembung v

G3.4 Kuning Berlendir Rata Cembung

G3.5 Kuning Berlendir Rata Cembung

G4.2 Kuning Berlendir Rata Cembung v

G4.5 Kuning Berlendir Rata Cembung v

G5.1 Orange Berlendir Rata Cembung v

G5.2 Kuning Berlendir Rata Cembung

G5.3 Kuning Berlendir Rata Cembung v

G5.4 Orange Berlendir Rata Cembung v

S1.3 Kuning Berlendir Rata Cembung

S1.4 Putih susu Tidak berlendir

Bergelombang Cembung S4.1 Putih susu Tidak

berlendir

Bergelombang Cembung v S4.2 Putih susu Tidak

berlendir

Bergelombang Cembung v

S4.3 Putih susu Berlendir Rata Rata v

S4.5 Putih susu Berlendir Rata Rata v

S5.1 Putih susu Berlendir Rata Rata

S5.4 Putih susu Berlendir Rata Rata v

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Lampiran 3 Isolar bakteri endofit dari tiga daerah

Gambar isolat bakteri endofit asal Cianjur

Gambar isolat bakteri endofit asal Garut

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Lampiran 4 Komposisi larutan yang digunakan Kalium Asetat

TAE (Tris-Asetat-EDTA) 50x

Buffer ekstrak

Kappa 2G Fast ReadyMix yang diproduksi oleh KAPABIOSYSTEM

3 M Kalium 5 M Asetat pH 4.8 242 g basa Tris 57.1 mL Asetat glasial 100 mL 0.5 M EDTA Ph 8 100 mM Tris HCl 10 mL 100 mM EDTA 20 mL 250 mM NaCl 5 mL 1 % SDS 5 mL 1 % PVP 1 g

DNA polimerase 0.5 U per 25 µL reaksi

Buffer PCR dNTPs 0.2 mM

MgCl2 1 mM dalam 1x Stabilizers

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Lampiran 7 Sekuen DNA isolat G42

GCCTTGAATGCAGTCCTATGCCCGCCGTGTTAANGAAGAAAGGCCCTTTCG GGATTTGTAAAAGTACCTTTCAAGCGGGGAAGGAAAGGCGGGTGAGGGTTAAATA ACCCTTGGCCGATTTGACCGTTACCCGGCAGAAGGAAGCACCGGGCTAACTTCCG GGGCCAGCAAGCCGCGGTAATTCGGAAGGGTGCAAAGCGTTAATTCGGAATTAAC TGGGCGTAAAGGCGCACGCAGGGCGGTTCTGTTAAGTCCAGATGTGAAATCCCCC GGGCTTAACCTGGGAACTGCATTTGAAACTGGCAGGCTTGAGTCTCGTAGAGGGG GGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTG GCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGC AAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGT TGTGCCCTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGA GTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTG GAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGCCTTGA

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