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Lampiran 1 Karakteristik koloni Bacillus sp. yang berhasil diisolasi dari 11 lokasi pengambilan sampel
Karakteristik koloni bakteri
Lokasi Sampel Kode isolat Bentuk Permukaan Warna Tepian Elevasi
GI-1 Tidak
beraturan Kering Krem
Berlekuk-lekuk Datar
GI-2 Bundar Berlendir Krem Rata Cembung
GI-3 Bundar Berlendir Krem Rata Cembung
GI-4 Bundar Berlendir Putih Rata Cembung
GI-5 Bundar Kering Putih keruh Rata Cembung
Ds. Kedung Dawa, Kec. Gabus Wetan,
Indramayu (6 isolat)
GI-6 Bundar Berlendir Coklat Rata Cembung
HI-1 Bundar Berlendir Krem Rata Timbul
HI-2 Bundar Berlendir Krem
kecoklatan Rata Cembung
HI-3 Bundar Berlendir Putih Rata Cembung
HI-4 Bundar Berlendir Krem Rata Cembung
HI-5 Tidak
beraturan Berlendir Putih keruh
Berlekuk-lekuk Datar
HI-6 Bundar Seperti berpati Putih Rata Cembung
HI-7 Tidak
beraturan Kering Putih keruh
Berlekuk-lekuk Timbul
HI-8 Bundar Seperti berpati Putih Rata Cembung
Ds. Sidadadi, Kec. Haurgeulis,
Indramayu (9 isolat)
HI-9 Bundar Agak kering, keriput Krem Rata Timbul
PI-1 Tidak
beraturan Berlendir Putih keruh
Berlekuk-lekuk Datar
PI-2 Bundar Berlendir Krem Rata Cembung
PI-3 Bundar Berlendir Putih keruh Rata Cembung
PI-4 Bundar Berlendir Krem Rata Cembung
PI-5 Tidak
beraturan Kering Krem
Berlekuk-lekuk Datar
PI-6 Bundar Berlendir Krem Rata Cembung
PI-7 Bundar Berlendir Krem Rata Cembung
PI-8 Bundar Berlendir Kuning Rata Cembung
PI-9 Tidak
beraturan Berlendir Krem Berombak Datar
Patrol, Indramayu (10 isolat)
PI-10 Bundar Kering, seperti berpati Putih keruh Rata Timbul SI-1 beraturan Tidak Kering Putih keruh Berlekuk-lekuk Timbul
SI-2 Bundar Berlendir Putih Rata Cembung
SI-3 Bundar Kering Putih keruh Rata Cembung
SI-4 Bundar Berlendir Krem Rata Cembung
SI-5 Bundar Berlendir Krem Rata Timbul
Ds. Mekar Gading, Kec. Sliyeg,
Indramayu (6 isolat)
SI-6 Bundar Agak kering, keriput Krem Rata Timbul
LI-1 Bundar,
menyebar Berlendir Putih Bergerigi Cembung
LI-2 Bundar Berlendir Krem Rata Cembung
LI-3 Bundar Berlendir Kuning Rata Cembung
Ds. Santi Kec. Losarang, Indramayu (4 isolat) LI-4 Tidak beraturan, menyebar
Kering Krem
Berlekuk-lekuk Timbul
TK-1 Bundar Berlendir Kuning Rata Cembung
TK-2 Tidak beraturan, seperti jari tangan Seperti berpati (starchy) Krem kekuningan Bercabang -cabang Timbul TK-3 Bundar Keriput dan berlendir Putih agak
transparan Rata Timbul
TK-4 Bundar Berlendir Putih keruh Rata Cembung
Ds. Cariumulya Kec. Telaga Sari,
Karawang (5 isolat)
TK-5 Tidak
beraturan Kering Krem
Berlekuk-lekuk Datar
TG-1 Bundar Berlendir Putih krem Rata Cembung
TG-2 Seperti bintang Berlendir Krem Berlekuk-lekuk Timbul
TG-3 Bundar Berlendir Agak
transparan Rata Timbul
TG-4 Bundar Kering, keriput Krem Rata Timbul
TG -5 Bundar Berlendir Putih keruh Rata Cembung
Tegal (6 isolat)
TG-6 Tidak
Karakteristik koloni bakteri Lokasi Sampel Kode
isolat Bentuk Permukaan Warna Tepian Elevasi
DM-1 Bundar Seperti berpati Putih Rata Cembung
DM-2 Bundar Agak kering, keriput Krem Rata Timbul
DM-3 Bundar Tidak berlendir Krem Rata Timbul
DM-4 Bundar Berlendir Putih Rata
Cembung, seperti tombol
DM-5 Tidak
beraturan Berlendir Putih
Berlekuk-lekuk Timbul
DM-6 Bundar Berlendir Krem Rata Cembung
Demak (7 isolat)
DM-7 Bundar Berlendir Krem Rata Cembung
RB-1 Bundar Berlendir Putih Rata Seperti
kawah RB-2
Tidak beraturan, menyebar
Berlendir Krem
Berlekuk-lekuk Timbul Rembang
(3 isolat)
RB-3 Bundar Berlendir Coklat Rata Timbul
KB-1 Bundar Berlendir Coklat Rata Timbul
KB-2 Tidak
beraturan Kering Krem
Berlekuk-lekuk Datar Kebumen (3 isolat) KB-3 Tidak beraturan, menyebar
Berlendir Krem
Berlekuk-lekuk Timbul
BY-1 Bundar Berlendir Krem Seperti
kerang Timbul BY-2 Seperti bintang Berlendir Putih keruh
Berlekuk-lekuk Timbul
BY-3 Bundar Berlendir Krem
Berlekuk-lekuk Datar Boyolali I
(4 isolat)
BY-4 Tidak
Lampiran 2 Kurva standar pengukuran AIA y = 0.0144x + 0.0157 R2 = 0.9921 0 0.1 0.2 0.3 0.4 0.5 0 10 20 30 4 Konsentrasi AIA (ppm) A b so rb a n si 5 1 0 n m 0
Lampiran 3 Kurva standar pertumbuhan isolat HI-2
y = 0.1938x + 0.1062 R2 = 0.9558 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 0 1 2 3 4 5 Jumlah sel/ml (x109) A bs or bans i 620 nm