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Lampiran 1. Prosedur analisis bahan aktif purwoceng dengan metode GC-MS
Analisis bahan aktif tanaman purwoceng menggunakan metode GC-MS (Chromatography-Mass Spectrophotometry) dilakukan di Laboratorium Pemeriksaan Doping dan Kesehatan Masyarakat, Jakarta.
Alat Gas Chromatography (Agilent Technologies) dengan panjang Column Capillary (Innowax) 30 m, diameter 0,25 mm, film thnkness 0,25 µm. Temperatur inlet diatur pada 250 0C dan dengan menggunakan gas pembawa helium. Prosedur pengujian diawali dengan mengatur temperatur oven dengan temperature awal 130 0C selama 2 menit, kemudian dinaikkan 60C/menit menjadi 170 0C selama 2 menit, 3 0C/menit sampai mencapai 2150C selama 1 menit, dan dinaikkan lagi 400C/menit menjadi 2500C selama 10 menit. Waktu ranning yang dibutuhkan adalah 20 menit dengan aliran tetap (constant flow) pada 1.5 µl/menit. Volume yang diinjeksikan sebanyak 5µg dengan perbandingan aliran 1 : 100 dihubungkan dengan auto sampler Mass Selective Detector (Agilent 5973, electron impact (IE)) dan Chemstation data system. Suhu sumber ion 2300C dan suhu interface 2800C serta suhu quadrupole adalah 1400C. Komponen terdeteksi berdasarkan waktu retensi puncak kromagram (% puncak area) dan spectra mass.
Lampiran 2. Data dan uji statistik jumlah dan prosentase jenis kelamin ikan nila Perlakuan Ulangan ∑ Sampel ∑ Jantan ∑ intersex ∑ Betina Prosentase (%) Jantan Intersex Betina
1 30 16 0 14 53.28 0 46.62 K (-) 2 30 17 0 13 56.62 0 43.29 3 30 14 0 16 46.62 0 53.28 Total 90 47 0 43 156.52 0 143.19 Rata-rata 30 15.,67 0 14,33 52,17 47,73 1 30 24 3 3 79.92 9.99 9.99 MT (+) 2 30 23 3 4 76.59 9.99 13.32 3 30 25 1 4 83.25 3.33 13.32 Total 90 72 7 11 239.76 23.31 36.63 Rata-rata 30 24 2,33 3,67 79,92 7,77 12,21 1 30 19 3 8 63.27 9.99 26.64 P10 2 30 21 0 9 69.93 0 29.97 3 30 20 5 5 66.6 16.65 16.65 Total 90 60 8 22 199.8 26.64 73.26 Rata-rata 30 20 2,67 7,33 66,6 8,88 24,42 1 30 22 3 5 73.26 9.99 16.65 P20 2 30 23 5 2 76.59 16.65 6.66 3 30 21 4 5 69.93 13.32 16.65 Total 90 66 12 12 219.78 39.96 39.96 Rata-rata 30 22 4 4 73,26 13,32 13,32 1 30 20 3 7 66.6 9.99 23.31 P30 2 30 20 4 6 66.6 13.32 19.98 3 30 22 4 4 73.26 13.32 13.32 Total 90 62 11 17 206.46 36.63 56.61 Rata-rata 30 20,67 3,67 5,67 68,82 12,21 18,87 Ulangan K (-) MT (+) P10 P20 P30 1 53,33 79,99 63,33 73,33 66,66 2 56,66 76,66 69,99 76,66 66,66 3 46,66 83,33 66,66 69,99 73,33 Rata-rata 52,22 80% 66,66 73,33 68,88
ANOVA ikan nila jantan ANOVA