BOGOR
2012
DAFTAR PUSTAKA
Achmadi AS. 2001. Kaji banding kualitas dan keutuhan membran plasma semen beku sapi pada setiap jalur distribusi [Skripsi]. Fakultas Kedokteran Hewan. Institut Pertanian Bogor.
Arifiantini I, Yusuf TL dan Graha N. 2005a. Recovery Rate dan Longivitas Pasca Thawing Semen Beku Sapi FH (Friesian Holstein) Menggunakan Berbagai Bahan Pengencer. Jakarta, Bul Peternakan Vol. 29 (2), 2005.
Arifiantini I, Yusuf TL, Indah O. 2005b. Kaji banding dua teknik pengemasan menggunakan tiga macam pengencer untuk pembekuan semen sapi Friesian Holstein (FH). Seminar Nasional Teknologi Pertanian dan Veteriner 2005.
Asturiano JF. 2007. Effect of sperm cryopreservation on the European eel sperm viability and spermatozoa morphology. Reprod Domest Anim 42:162-166. Ball PHJ, Peters AR. 2004. Reproduction in Cattle 3rd edition. United Kingdom:
Blackwell Publishing Ltd.
Barth A D, Oko RJ. 1989. Abnormal Morphology of Bovine Spermatozoa. USA: Iowa State University Press.
Bearden HJ, Fuquay JW. 2000. Applied Animal Reproduction 5th ed. Missisippi State University. New Jersey. Pp 24-143.
Bearden HJ, Fuquay JW, Willard ST. 2004. Applied Animal Reproduction. 6th Ed. Pearson Education Inc: New Jersey.
Birkhead TR, Hosken DJ, Pitnick S. 2009. Sperm Biology: An Evolutionary Perspective. United Kingdom: Elsevier Ltd.
Björndahl I, Söderlund I, Johansson S, Mohammadieh M, Pourian MR, Kvist U. 2004. Why the WHO recommendation for eosin-Nigrosin staining techniques for human sperm vitality assessment must change. J Androl 2004;25:671– 8
Blottner S, Warnke C, Tuchscherer A, Heinen V, Torner H. 2001. Morphological and functional changes of stallion spermatozoa after cryopreservation during breeding and non-breeding season. Anim Reprod Sci.65:75-88. Buhr MM, Curtis EF, Kakuda SN. 1994. Composition and behaviorof head
membrane lipids of fresh and cryopreserved boar sperm. Cryobiology. 31:224-238.
Campbell JR, Campbell KL, Kenealy MD. 2003. Artificial Insemination in : Animal Sciences 4th Edition. New York:Mc Graw-Hill.
Challah T, Brillard JP. 1998. Comparison of assessment of fowl sperm viability by eosin-Nigrosin and dual fluorescence (SYBR-14/PI). Theriogenology 1998;50:487–93.
Colenbrander, Fazeli AR, Van Buiten A, Parlevliet J, Gadella BM. 1992. Assesment of sperm cell membran integrity in the horse. Acta Ve. Scand Suppl. 88: 49-58.
Cowell RL, Tyler RD. 2002. Diagnostic Cytology and Hematology Second edition. USA: Mosby, Inc.
Eiriani K, Boediono A, Djuwita I, Sumarsono SH, Al-Azhar. 2008. Development of domestic cat embryo produced by preserved sperms. J Biosciences 15:155-160.
Fikar Samsul, Ruhyadi D. 2010. Buku Pintar Beternak dan Bisnis Sapi Potong. Jakarta: AgroMedia Pustaka.
Foote RH, 2003: Fertility estimation: a review of past experience and future prospects. Anim Reprod Sci 75 119–139.
Gage MJG, Macfarlane CP, Yeates S, Ward RG, Searle JB, Parker GA. 2004. Spermatozoal traits and sperm competition in Atlantic salmon: relative sperm velocity is the primary determinant of fertilization success. Curr Biol. 14: 44-47.
Garner DL, Hafez ESE. 2000. Spermatozoa and seminal plasma. Page 96-109 in Reproduction in Farm Animals. 7th edition. Lippincott Williams and William: Philadelphia, PA.
Gardner DK, Weissman A, Howless M, Shoham Zeev. 2001. Textbook of Assisted Reproductive Techniques Laboratory and Clinical Perspectives. United Kingdom: Martin Dunitz Ltd.
Gordon Ian. 2005. Reproductives Technologies in Farm Animals. UK: Cromwell Press.
Graham JK. 1996. Analysis of stallion semen and its relation to fertility. Vet Clin North Am, Equine Pract. 12:119-130.
Hafez ESE. 2000. Preservation and Cryopreservation of Gametes and Embryos. In Reproduction in Farm Animals. 7th ed B Hafez/ESE Hafez, editor. USA: Lippincott Wiliams & Wilkins.
Hammerstedt RH , Graham JK, Kunze E.1990. Analysis of sperm cell viability, acrosomal integrity and mitochondrial function using flow cytometry. Biol Repord 1990;43:55– 64.
He S, Woods C. 2004. Changes in motility, ultrastructure, and fertilization capacity of striped bass Morone saxatilis spermatozoa following cryopreservation. Aquaculture 236 (2004) 677–686
Mazur P, Schneider V. 1984. Osmotic consequences of cryoprotectant permeability andits relation to the survival of frozen-thawed embryos. Theriogenology 1984;21:68-79.
Morell JM, Rodriguez-Martinez H. 2009. Biomimetic Techniques for Improving Sperm Quality in Animal Breeding: A Review. The Open Andrology J 2009, Volume 1.
Nazlie C S. 2004. Kajian kualitas spermatozoa kucing asal epididimys dan duktus deferens setelah preservasi pada suhu 4C [tesis]. Bogor: Sekolah Pascasarjana, Institut Pertanian Bogor.
Nilna. 2010. Standar Operasional Pekerjaan Prosesing Semen. [terhubung berkala] http://disnak.sumbarprov.go.id/?disnak=profil&id=85 [18 Januari 2012].
Park JE, Graham JK. 1992. Effects of cryopreservation procedures on sperm membranes. J. Theriogenology. 38: 2009-222.
Parks JE, Lynch DV, 1992. Lipid composition and thermotropic phase behavior of boar, bull, stallion and rooster sperm membranes. Cryobiology 29, 255– 266.
Rigby SL, Brinsko SP, Cochran M, Blanchard TL, Love CC, Varner DD. 2001. Advances in cooled semen technologies: seminal plasma and semen extender. Anim Reprod Sci. 68:171-180.
[SNI]. 2005. Semen beku sapi SNI 01-4869.1-2005.[terhubung berkala]
pphp.deptan.go.id/…/5.pdf [15 Januari 2012].
Soeradi O. 2004. Radikal bebas pada pria infertil. Di dalam: Paradigma Terkini Genetika dan Reproduksi. Jakarta: Departemen Biologi Kedokteran. Fakultas Kedokteran, UI. Hlm 95-101.
Sudarmono AS, Sugeng YB. 2008. Sapi Potong Cet.17 Edisi Revisi. Jakarta: Penebar Swadaya.
Syarif EK, Harianto B. 2011. Buku Pintar Beternak dan Bisnis Sapi Perah.Jakarta: AgroMedia Pustaka.
Szasz F, Sirivaidyapong S, Chen FP, Voorhout WF, Marks A, Colenbrander B, Solti L, Gadella BM. 2000. Detection of calcium ionophore induced membrane changes in dog sperm as a simple method to predict the cryopreservability of dog semen. Mol Reprod Dev. 55:289-298.
Tamuli M, Watson P. 1994. Use of a simple staining technique to distinguish acrosomal changes in the live sperm sub-population Anim Reprod Sci 1994. 35:247-254.
[WHO] World Health Organization. 1999. WHO laboratory manual for the examination of human semen and sperm cervical mucus interactions. 4th edn. Cambridge University Press, Cambridge, UK.
Wundrich K, Paasch V, Leicht M, Glander HJ. 2006. Activaton of caspaces in human spermatozoa duringcryopreservation and immunoblot study. Cell Tissue Bank 7:81-90.
Yulnawati, Setiadi MA. 2005. Motilitas dan Keutuhan Membran Plasma Spermatozoa Epididimis Kucing Selama Penyimpanan Pada Suhu 4°C. Media Kedokteran Hewan Vol. 21, No. 3, September 2005.
LAMPIRAN
Lampiran 1 Persentase Motilitas dan Viabilitas Spermatozoa Sapi Friesian Holstein Waktu (Jam) Sampel Rata-rata 1 2 3 4 5 6 7 8 9 10 0 50-45 50 50-45 55-50 50 55-50 55-50 50-45 50-45 50-45 50.00±0.00 81.63 84.80 84.89 80.43 85.71 77.70 83.70 76.87 83.69 84.43 82.39±3.12 1 45-40 45-40 40 45 40 45 40 40 45 40 41.88±2.58 78.43 77.61 78.60 71.75 76.15 72.18 74.26 68.87 73.34 72.67 74.39± 3.23 2 30-25 30 30 40-35 35 30 35 30 40-35 30 31.43±2.44 69.05 66.84 74.70 68.02 65.97 63.24 64.86 63.13 61.96 57.38 65.52 ±4.66 3 15 25 20 20 25 20 25 15 10 5 18.00 ±6.75 62.23 65.02 70.19 65.33 58.86 59.09 62.22 60.29 61.83 41.15 60.62 ±7.63 4 5 10 5 10 5 5 10 5 5 0 6.00 ± 3.16 47.27 52.36 46.89 55.21 54.46 55.37 53.93 51.15 42.36 30.61 48.96 7.74 5 0 0 0 0 0 0 0 0 0 0 0.00 ± 0.00 44.36 44.72 40.94 43.52 42.35 44.23 46.28 40.52 39.87 0 42.98 2.18 Lampiran 2 Persentase Motilitas dan Viabilitas Spermatozoa Sapi Simmental
Waktu (Jam) Sampel Rata-rata 1 2 3 4 5 6 7 8 9 10 0 40 45 45 45 45 40 45 45 40 45 43.50 ±2.42 55.50 60.49 60.00 66.97 59.60 61.54 65.71 63.64 63.25 68.97 62.57 ±3.98 1 30-25 35-30 35-30 35-30 35-30 35 35 30 35 35 34.00 ±2.24 52.07 57.80 52.41 52.17 55.56 58.42 61.02 55.32 55.78 52.52 55.31 ±3.08 2 20-15 15 20-15 25-20 25 20-15 20 25 25 25 22.50 ±4.18 49.96 51.79 44.60 50.68 52.41 43.42 36.52 61.07 55.19 46.07 49.14 ±6.84 3 15 15 10 15 15 15 10 10 10 10 12.50 ±2.64 40.14 37.88 39.77 45.83 36.70 42.59 35.71 46.34 43.86 47.97 41.68 ±4.28 4 5 5 5 10-5 5 10-5 5 5 0 0 3.75 ± 2.31 44.09 42.78 37.97 41.22 38.46 43.24 36.36 43.92 27.59 40.85 39.65 ±5.00 5 0 0 0 0 0 0 0 0 0 0 0.00 ± 0.00 35.65 30.09 28.11 37.28 32.45 35.84 35.87 33.33 - - 33.58±3.20
Lampiran 3 Persentase Motilitas dan Viabilitas Spermatozoa Sapi Brahman Waktu (Jam) Sampel Rata-rata 1 2 3 4 5 6 7 8 9 10 0 45 45 45 40 45 40 45 45-40 40 45 43.33 ± 2.50 60.44 64.84 74.58 63.86 64.60 58.14 68.58 66.23 60.84 64.41 64.65±4.62 1 35-30 35 30 30 35-30 35-30 35 35 35-30 35 33.33 ± 2.58 57.42 68.71 61.17 65.52 57.84 58.66 63.58 65.13 67.07 65.98 63.11 ±4.07 2 30-25 30-25 20 25-20 25-20 25 30-25 30-25 30-25 30-25 22.50 ± 3.54 59.79 65.50 54.46 65.77 52.43 54.39 52.00 58.50 60.36 67.44 59.06± 5.73 3 20 20-15 15 15-10 20-15 10 15 10 15-10 10 13.33 ± 4.08 51.35 48.31 40.07 51.96 51.60 38.24 49.16 49.07 53.71 52.77 48.62 ±5.29 4 10-5 10 10 5 5 5 5 0 5 0 5.00 ± 3.54 45.45 46.15 42.93 42.86 39.01 37.19 45.99 46.75 49.18 37.18 43.27 ±4.22 5 0 0 0 0 0 0 0 0 0 0 0.00 ± 0.00 42.23 37.16 38.51 39.71 39.00 26.19 33.34 - 34.25 - 32.26±12.97 Lampiran 4 Analisis Motilitas Sapi
Jam 0
hasil penelitian 21:20 Thursday, June 18, 2012 10 The ANOVA Procedure
Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 11 The ANOVA Procedure
Dependent Variable: jam0
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 0.02482667 0.01241333 22.33 <.0001 Error 27 0.01501000 0.00055593
Corrected Total 29 0.03983667
R-Square Coeff Var Root MSE jam0 Mean 0.623211 5.189600 0.023578 0.454333
Source DF Anova SS Mean Square F Value Pr > F jenis 2 0.02482667 0.01241333 22.33 <.0001 hasil penelitian 21:20 Thursday, June 18, 2012 12 The ANOVA Procedure
Duncan's Multiple Range Test for jam0
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 0.000556 Number of Means 2 3 Critical Range .02164 .02273
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 0.49500 10 f B 0.43500 10 s B
B 0.43300 10 b
Jam 1
hasil penelitian 21:20 Thursday, June 18, 2012 13 The ANOVA Procedure Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 14 The ANOVA Procedure
Dependent Variable: jam1
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 0.05554167 0.02777083 54.29 <.0001 Error 27 0.01381250 0.00051157
Corrected Total 29 0.06935417
R-Square Coeff Var Root MSE jam1 Mean 0.800841 6.297356 0.022618 0.359167
Source DF Anova SS Mean Square F Value Pr > F jenis 2 0.05554167 0.02777083 54.29 <.0001 hasil penelitian 21:20 Thursday, June 18, 2012 15 The ANOVA Procedure
Duncan's Multiple Range Test for jam1
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 0.000512 Number of Means 2 3 Critical Range .02075 .02181
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 0.42000 10 f B 0.33000 10 b B
B 0.32750 10 s
Jam 2
hasil penelitian 21:20 Thursday, June 18, 2012 16 The ANOVA Procedure
Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 17 The ANOVA Procedure
Dependent Variable: jam2
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 0.06481167 0.03240583 25.15 <.0001 Error 27 0.03479250 0.00128861
Corrected Total 29 0.09960417
R-Square Coeff Var Root MSE jam2 Mean 0.650692 13.58886 0.035897 0.264167
Source DF Anova SS Mean Square F Value Pr > F jenis 2 0.06481167 0.03240583 25.15 <.0001 hasil penelitian 21:20 Thursday, June 18, 2012 18 The ANOVA Procedure
Duncan's Multiple Range Test for jam2
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 0.001289 Number of Means 2 3 Critical Range .03294 .03461
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 0.32350 10 f B 0.25900 10 b C 0.21000 10 s
Jam 3
hasil penelitian 21:20 Thursday, June 18, 2012 25 The ANOVA Procedure
Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 26 The ANOVA Procedure
Dependent Variable: jam3
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 0.01608500 0.00804250 3.67 0.0388 Error 27 0.05912250 0.00218972
Corrected Total 29 0.07520750
R-Square Coeff Var Root MSE jam3 Mean 0.213875 31.51143 0.046794 0.148500
Source DF Anova SS Mean Square F Value Pr > F jenis 2 0.01608500 0.00804250 3.67 0.0388 hasil penelitian 21:20 Thursday, June 18, 2012 27 The ANOVA Procedure
Duncan's Multiple Range Test for jam3
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 0.00219 Number of Means 2 3 Critical Range .04294 .04511
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 0.18000 10 f A B A 0.14050 10 b B B 0.12500 10 s Jam 4
hasil penelitian 21:20 Thursday, June 18, 2012 28 The ANOVA Procedure Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 29 The ANOVA Procedure
Dependent Variable: jam4
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 0.00105167 0.00052583 0.55 0.5841 Error 27 0.02588500 0.00095870
Corrected Total 29 0.02693667
R-Square Coeff Var Root MSE jam4 Mean 0.039042 58.79039 0.030963 0.052667
Source DF Anova SS Mean Square F Value Pr > F jenis 2 0.00105167 0.00052583 0.55 0.5841 hasil penelitian 21:20 Thursday, June 18, 2012 30 The ANOVA Procedure
Duncan's Multiple Range Test for jam4
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 0.000959 Number of Means 2 3 Critical Range .02841 .02985
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 0.06000 10 f A A 0.05250 10 b A A 0.04550 10 s Jam 5
hasil penelitian 21:20 Thursday, June 18, 2012 31 The ANOVA Procedure
Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 32 The ANOVA Procedure
Dependent Variable: jam5
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 0 0 . .
Error 27 0 0 Corrected Total 29 0
R-Square Coeff Var Root MSE jam5 Mean 0.000000 . 0 0
Source DF Anova SS Mean Square F Value Pr > F jenis 2 0 0 . .
hasil penelitian 21:20 Thursday, June 18, 2012 33 The ANOVA Procedure
Duncan's Multiple Range Test for jam5
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 0 Number of Means 2 3 Critical Range 0 0
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 0 10 b A
A 0 10 f A
A 0 10 s
Lampiran 5 Analisis Viabilitas Sapi
Jam 0
hasil penelitian 21:20 Thursday, June 18, 2012 34 The ANOVA Procedure Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 35 The ANOVA Procedure
Dependent Variable: jam0
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 2372.468409 1186.234205 75.91 <.0001 Error 27 421.912515 15.626389
Corrected Total 29 2794.380925
R-Square Coeff Var Root MSE jam0 Mean 0.849014 5.657872 3.953023 69.86767
Source DF Anova SS Mean Square F Value Pr > F jenis 2 2372.468409 1186.234205 75.91 <.0001 hasil penelitian 21:20 Thursday, June 18, 2012 36 The ANOVA Procedure
Duncan's Multiple Range Test for jam0
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 15.62639 Number of Means 2 3 Critical Range 3.627 3.811
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 82.386 10 f B 64.651 10 b B
B 62.566 10 s
Jam 1
hasil penelitian 21:20 Thursday, June 18, 2012 37 The ANOVA Procedure
Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 38 The ANOVA Procedure
Dependent Variable: jam1
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 1840.883230 920.441615 75.69 <.0001 Error 27 328.353250 12.161231
Corrected Total 29 2169.236480
R-Square Coeff Var Root MSE jam1 Mean 0.848632 5.426256 3.487296 64.26707
Source DF Anova SS Mean Square F Value Pr > F jenis 2 1840.883230 920.441615 75.69 <.0001 hasil penelitian 21:20 Thursday, June 18, 2012 39 The ANOVA Procedure
Duncan's Multiple Range Test for jam1
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 12.16123 Number of Means 2 3 Critical Range 3.200 3.362
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 74.388 10 f B 63.107 10 b C 55.306 10 s
Jam 2
hasil penelitian 21:20 Thursday, June 18, 2012 40 The ANOVA Procedure
Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 41 The ANOVA Procedure
Dependent Variable: jam2
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 1360.372722 680.186361 20.13 <.0001 Error 27 912.462982 33.794925
Corrected Total 29 2272.835704
R-Square Coeff Var Root MSE jam2 Mean 0.598535 10.03890 5.813340 57.90817
Source DF Anova SS Mean Square F Value Pr > F jenis 2 1360.372722 680.186361 20.13 <.0001 hasil penelitian 21:20 Thursday, June 18, 2012 42 The ANOVA Procedure
Duncan's Multiple Range Test for jam2
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 33.79493 Number of Means 2 3 Critical Range 5.334 5.604
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 65.516 10 f B 59.064 10 b C 49.144 10 s
Jam 3
hasil penelitian 21:20 Thursday, June 18, 2012 43 The ANOVA Procedure
Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 44 The ANOVA Procedure
Dependent Variable: jam3
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 1836.406706 918.203353 26.33 <.0001 Error 27 941.568195 34.872896
Corrected Total 29 2777.974901
R-Square Coeff Var Root MSE jam3 Mean 0.661060 11.73847 5.905328 50.30747
Source DF Anova SS Mean Square F Value Pr > F jenis 2 1836.406706 918.203353 26.33 <.0001 hasil penelitian 21:20 Thursday, June 18, 2012 45 The ANOVA Procedure
Duncan's Multiple Range Test for jam3
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 34.8729 Number of Means 2 3 Critical Range 5.419 5.693
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 60.620 10 f B 48.623 10 b C 41.679 10 s
Jam 4
hasil penelitian 21:20 Thursday,
June 18, 2012 46
The ANOVA Procedure Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 47 The ANOVA Procedure
Dependent Variable: jam4
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 440.660632 220.330316 6.44 0.0052 Error 27 923.902635 34.218616
Corrected Total 29 1364.563268
R-Square Coeff Var Root MSE jam4 Mean 0.322932 13.30695 5.849668 43.95950
Source DF Anova SS Mean Square F Value Pr > F jenis 2 440.6606322 220.3303161 6.44 0.0052 hasil penelitian 21:20 Thursday, June 18, 2012 48 The ANOVA Procedure
Duncan's Multiple Range Test for jam4
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 34.21862 Number of Means 2 3 Critical Range 5.368 5.639
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 48.960 10 f B 43.269 10 b B
B 39.649 10 s
Jam 5
hasil penelitian 21:20 Thursday, June 18, 2012 49 The ANOVA Procedure
Class Level Information Class Levels Values jenis 3 b f s
Number of Observations Read 30 Number of Observations Used 30
hasil penelitian 21:20 Thursday, June 18, 2012 50 The ANOVA Procedure
Dependent Variable: jam5
Sum of
Source DF Squares Mean Square F Value Pr > F Model 2 791.201416 395.600708 1.82 0.1809 Error 27 5858.942413 216.997867
Corrected Total 29 6650.143830
R-Square Coeff Var Root MSE jam5 Mean 0.118975 46.72529 14.73085 31.52650
Source DF Anova SS Mean Square F Value Pr > F jenis 2 791.2014162 395.6007081 1.82 0.1809 hasil penelitian 21:20 Thursday, June 18, 2012 51 The ANOVA Procedure
Duncan's Multiple Range Test for jam5
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05
Error Degrees of Freedom 27 Error Mean Square 216.9979 Number of Means 2 3 Critical Range 13.52 14.20
Means with the same letter are not significantly different. Duncan Grouping Mean N jenis
A 38.680 10 f A
A 29.039 10 b A