SEBAGAI INOVASI TERAPI HIPERTENSI
2.7 Human Effect Matrix
Human Effect Matrix membahas mengenai toksikologi serta efek samping yang dapat ditimbulkan suatu obat terhadap tubuh manusia. Berdasarkan studi review yang dilakukan oleh Harwood et al[31] mengenai tinjauan toksikologi dari quercetin, disebutkan bahwa pemberian dosis tinggi dari quercetin tidak memiliki efek toksik terhadap tubuh manusia. Selain itu, review dari berbagai studi single/double-blind studies maupun
observational studies menunjukkan bahwa quercetin tidak memiliki efek samping merugikan bagi tubuh manusia. Oleh karena itu, quercetin dinyatakan sebagai substansi GRAS (Generally Recognized As Safe).[32]
3. SIMPULAN
Berdasarkan pemaparan sebelumnya, quercetin terenkapsulasi SLN dengan modifikasi PEG sangat berpotensi untuk digunakan sebagai inovasi terapi pada penderita hipertensi. Walaupun studi preklinik di bidang farmakologi belum dilakukan, adanya potensi quercetin dalam menurunkan tekanan darah melalui berbagai prinsip mekanisme kerja dapat dijadikan terobosan terbaru dalam pengembangan terapi hipertensi.
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ABSTRAK
Latar Belakang: Timbal yang mencemari makanan dapat menyebabkan keracunan timbal
sub-akut. Timbal diperkirakan berhubungan dengan inhibisi pada pyrimidine-5' nucleotidase dan menyebabkan anemia hemolitik. Terapi saat ini dinilai belum efektif menangani keracunan timbal. Lidah mertua (Sansevieria trifasciata Laurentii) memiliki kandungan pregnane glycoside dan vitamin C sebagai antioksidan yang mampu mengikat logam berat timbal.
Tujuan: Mengetahui bagaimana pengaruh pemberian ekstrak daun lidah mertua terhadap
jumlah eritrosit darah tikus (Rattus norvegicus strain wistar) yang telah diinduksi oleh timbal per-oral.
Metode Penelitian: Penelitian ini menggunakan desain True Experimental Post Test Only
Group Design selama 15 hari. Lima kelompok tikus, yaitu kelompok tanpa perlakuan (K-), kelompok yang diinduksi timbal (K+), serta kelompok P1; P2; P3 diberi induksi timbal dan ektrak daun lidah mertua. Induksi timbal menggunakan larutan timbal asetat dosis 15 mg/ 100 gBB/ hari. Satu jam kemudian, ekstrak daun lidah mertua diberikan per-oral pada kelompok P1; P2; dan P3 dengan dosis secara berurutan 8,3; 41; 83 mg/ kgBB/ hari. Pengambilan sampel darah tikus melalui ventrikel kemudian dilakukan uji kadar timbal menggunakan mesin hitung darah lengkap Horiba MedicalTM ABX Micros 60 buatan Prancis.
Hasil Penelitian dan Pembahasan: Hasil dari uji komparasi Oneway ANOVA didapatkan
nilai signifikansi p=0.69 yang berarti penelitian ini tidak terdapat perbedaan bermakna antara jumlah eritrosit pada seluruh kelompok.
Kesimpulan: Ekstrak daun lidah mertua (Sansevieria trifasciata Laurentii) tidak
memberikan perbedaan bermakna terhadap jumlah eritrosit tikus (Rattus norvegicus strain wistar) yang diinduksi timbal secara per-oral. Perlu penelitian lebih lanjut dengan dosis yang lebih tinggi dan dengan teknik yang lebih baik
Kata Kunci: jumlah eritrosit, Sansevieria trifasciata Laurentii, timbal asetat