5.1. Kesimpulan
1. Enzim DPEase yang dimurnikan menggunakan kolom 6xhis tag dengan satu kali purifikasi langsung dalam keadaan aktif. Dikonfirmasi melalui elektroforesis SDS-PAGE dan uji aktivitas enzim. Menurut hasil elektroforesis, enzim DPEase pada penelitian ini muncul dalam pita tunggal dengan massa molekul 32 kDa yang menunjukkan keberhasilan pemurnian menghasilkan aktivitas spesifik 5,09 U/mg.
2. DPEase murni menunjukkan aktivitas optimal pada pH 8,5 dan suhu 45°C. Aktivitas DPEase ditingkatkan dengan ketersediaan kofaktor, Co² +, Mn² +, Fe² +, Ni² + dan dihambat di hadapan Mg² +, Mo² +, Cu² +, Ca² +, Ca² +, Zn² +.
5.2. Saran
Untuk industrialisasi enzim DPEase dibutuhkan upaya perbaikan. Penambahan kofaktor Fe2+ bisa digunakan untuk menjawab persoalan itu. Fe2+ sangat direkomendasikan untuk aplikasinya di industri makanan..
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