40
Lampiran 2. Perhitungan dosis serbuk daun kemangi
Rumus perhitungan konsentrasi serbuk daun kemangi untuk masing-masing dosis yang dilarutkan dalam akuades, sebagai berikut: Konsentrasi = 1. Dosis 1 (0,25 g/kg BB) Konsentrasi = = 12,5 g/ml 2. Dosis 2 (0,50 g/kg BB) Konsentrasi = = 25 g/ml 3. Dosis 3 (1 g/kg BB) Konsentrasi = = 50 g/ml Keterangan :
Konsentrasi (g/ml) : Konsentrasi larutan serbuk daun kemangi yang akan dilarutkan dengan per 1 ml larutan akuades.
Dosis (g/kg BB) : Dosis yang digunakan untuk perlakuan BB (g) : Berat badan tikus sebagai acuan (200 g) V (ml) : Volume lambung mencit acuan (4 ml)
Lampiran 3. Kurva standar TEP
LT 4. Rerata absorbansi kurva standar TEP
No Volume (µl) Absorbansi Rerata
MDA H2O TBA TCA A B Absorbansi
0 0 100 500 250 0.005 0.003 0.0040 1 10 90 500 250 0.011 0.009 0.0100 2 20 80 500 250 0.016 0.015 0.0155 3 30 70 500 250 0.019 0.019 0.0190 4 40 60 500 250 0.021 0.02 0.0205 5 50 50 500 250 0.021 0.022 0.0215 6 60 40 500 250 0.024 0.027 0.0255 7 70 30 500 250 0.029 0.032 0.0305 8 80 20 500 250 0.031 0.037 0.0340 9 90 10 500 250 0.036 0.041 0.0385 10 100 0 500 250 0.044 0.045 0.0445 Kurva Standar TEP
42
Lampiran 4. Analisis data siklus estrus Analisis Descriptive Siklus Estrus
one-way ANOVA Siklus Estrus
44
Tukey Estrus
Tukey Metestrus
Lampiran 5. Analisis data kadar MDA serum Homogentitas MDA Serum
one-way ANOVA
46 Tukey
Lampiran 6. Analisis data kadar MDA ovarium one-way ANOVA
48
Lampiran 7. Analisis data kadar MDA serum dan ovarium one-way ANOVA
two-way ANOVA
BAB V
KESIMPULAN DAN SARAN 5.1 Kesimpulan
Pemberian serbuk daun kemangi berpengaruh terhadap durasi fase proestrus, estrus, dan metestrus tikus premenopause kelompok kontrol dibanding dengan kelompok perlakuan. Serbuk daun kemangi tidak berpengaruh secara signifikan pada fase diestrus tikus premenopause. Secara garis besar serbuk daun kemangi memperpendek durasi siklus estrus tikus premenopause yang diindikasi sebagai upaya untuk mengembalikan durasi siklus estrus seperti pada tikus usia produktif. Serbuk daun kemangi berpengaruh terhadap penurunan kadar MDA baik kadar MDA di serum maupun di ovarium. Penurunan paling signifikan terjadi pada kelompok kontrol dibanding dengan kelompok perlakuan dosis 3.
5.2 Saran
Adapun saran yang dapat dilakukan untuk penelitian selanjutnya yaitu:
1. Perlu adanya penelitian lebih lanjut dengan rentang waktu pemberian serbuk daun kemangi yang lebih lama.
2. Perlu adanya penelitian lebih lanjut dengan pemberian dosis serbuk daun kemangi yang berbeda.
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