BAB V KESIMPULAN DAN SARAN
B. Saran
1. Diperlukan pengkajian studi lanjut untuk penambahan dosis pada setiap perlakuan tindakan klinis supaya kadar IL-6 dapat menurun lebih signifikan
2. Diperlukan pemeriksaan kuantitatif kadar kandungan senyawa pada daun beruwas laut
3. Diperlukan studi lanjut tentang penggunaan ekstrak beruwas laut pada tatanan klinis lain.
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LAMPIRAN 1
Perhitungan Dosis Antibiotik Flucloxacillin
1. 𝐾𝑜𝑛𝑣𝑒𝑟𝑠𝑖 𝐵𝐵 𝑚𝑎𝑛𝑢𝑠𝑖𝑎 𝑘𝑒 𝑇𝑖𝑘𝑢𝑠 = 𝑁𝑖𝑙𝑎𝑖 𝐾𝑜𝑛𝑣𝑒𝑟𝑠𝑖 × 𝐵𝑒𝑟𝑎𝑡 𝐸𝑡𝑖𝑘𝑒𝑡
= 0,018 × 𝐵𝑒𝑟𝑎𝑡 𝐸𝑡𝑖𝑘𝑒𝑡
= 0,018 × 500 𝑚𝑔
= 9 𝑚𝑔/250 𝑔𝑟𝐵𝐵
2. 𝐷𝑜𝑠𝑖𝑠 𝑝𝑒𝑟 𝐵𝑒𝑟𝑎𝑡 𝑇𝑖𝑘𝑢𝑠 = 𝐵𝑒𝑟𝑎𝑡 𝑇𝑖𝑘𝑢𝑠
𝐵𝑒𝑟𝑎𝑡 𝑀𝑎𝑘𝑠𝑖𝑚𝑎𝑙 𝑇𝑖𝑘𝑢𝑠× 𝐷𝑜𝑠𝑖𝑠 𝐾𝑜𝑛𝑣𝑒𝑟𝑠𝑖
𝐷𝑜𝑠𝑖𝑠 𝑝𝑒𝑟 𝐵𝑒𝑟𝑎𝑡 𝑇𝑖𝑘𝑢𝑠=220 𝑔𝑟𝑎𝑚
250 𝑔𝑟𝑎𝑚× 9 𝑚𝑔
𝐷𝑜𝑠𝑖𝑠 𝑝𝑒𝑟 𝐵𝑒𝑟𝑎𝑡 𝑇𝑖𝑘𝑢𝑠=220 𝑔𝑟𝑎𝑚
250 𝑔𝑟𝑎𝑚× 7,92 𝑚𝑔
3. 𝑉𝑜𝑙𝑢𝑚𝑒 𝑙𝑎𝑟𝑢𝑡𝑎𝑛 𝑠𝑒𝑑𝑖𝑎𝑎𝑛 = 𝐽𝑢𝑚𝑙𝑎ℎ 𝑡𝑖𝑘𝑢𝑠/(𝑚𝑙) × (𝐷𝑜𝑠𝑖𝑠/𝐵𝐵) 𝑉𝑜𝑙𝑢𝑚𝑒 𝑙𝑎 = 5 (𝑚𝑙)
1 𝑚𝑙 × 7,92 𝑚𝑔 = 39,6 𝑚𝑔/𝑚𝑙
4. 𝐵𝑒𝑟𝑎𝑡 𝑜𝑏𝑎𝑡 𝑚𝑢𝑟𝑛𝑖 = 𝑏𝑒𝑟𝑎𝑡 𝑟𝑎𝑡𝑎−𝑟𝑎𝑡𝑎 𝑜𝑏𝑎𝑡
𝑏𝑒𝑟𝑎𝑡 𝐸𝑡𝑖𝑘𝑒𝑡 × 𝑉𝑜𝑙𝑢𝑚𝑒 𝑙𝑎𝑟𝑢𝑡𝑎𝑛 𝑠𝑒𝑑𝑖𝑎𝑎𝑛
= 605 𝑚𝑔
500 𝑚𝑔× 39,6
= 47,9 𝑚𝑔/5 𝑚𝑙
= 9,59 𝑚𝑔 𝑑𝑜𝑠𝑖𝑠 𝑎𝑛𝑡𝑖𝑏𝑖𝑜𝑡𝑖𝑘 𝑝𝑒𝑟 𝑡𝑖𝑘𝑢𝑠
5. 𝐽𝑢𝑚𝑙𝑎ℎ 𝑙𝑎𝑟𝑢𝑡𝑎𝑛 𝑦𝑎𝑛𝑔 𝑏𝑒𝑟𝑖𝑘𝑎𝑛 𝑝𝑒𝑟ℎ𝑒𝑤𝑎𝑛 = 𝐵𝑒𝑟𝑎𝑡 ℎ𝑒𝑤𝑎𝑛 𝐵𝑒𝑟𝑎𝑡 𝑀𝑎𝑥𝑖𝑚𝑎𝑙 ℎ𝑒𝑤𝑎𝑛
=220 𝑔𝑟𝑎𝑚 250 𝑔𝑟𝑎𝑚
= 0,88 𝑚𝑙 𝑑𝑖𝑔𝑒𝑛𝑎𝑝𝑘𝑎𝑛 𝑚𝑒𝑛𝑗𝑎𝑑𝑖 (0,9 𝑚𝑙)
Nb : 1 ml/9,59 mg
9,59 mg x 0,9 ml= 8,9 mg (Didalam 0,9 ml terdapat 8,9 mg kandungan antibiotic flucloxacillin)
LAMPIRAN 2
Perhitungan Dosis Ekstrak Daun Beruwas Laut Pada Hewan Coba
DOSIS EKSTRAK
1. Menentukan konsentrasi sediaan. (Berdasarkan dosis dan rute pemberian)
Dosis : 400 mg/kbBB
Rute pemberian per oral (konsentrasi 1% = 1 ml
100 grBB ) Rumus :
𝐾𝑜𝑛𝑠𝑒𝑛𝑡𝑟𝑎𝑠𝑖 𝐸𝐵𝐿 = 𝐷𝑜𝑠𝑖𝑠 𝐸𝐵𝐿
% 𝑃𝑒𝑚𝑏𝑒𝑟𝑖𝑎𝑛
400 𝑚𝑔/𝐾𝑔𝐵𝐵
1 𝑚𝑙/100𝑔𝑟𝐵𝐵 = 400 𝑚𝑔
1000 𝑔𝑟×100 𝑔𝑟 1 𝑚𝑙
400 𝑚𝑔
10 𝑔𝑟 ×1 𝑔𝑟
1 𝑚𝑙 = 40 mg/ml per 100gr BB
𝐾𝑜𝑛𝑠𝑒𝑛𝑡𝑟𝑎𝑠𝑖 = 40 𝑚𝑔/𝑚𝑙
= 4000 𝑚𝑔/100𝑚𝑙
= 4 𝑔𝑟/100𝑚𝑙
= 4 %𝑏/𝑣
2. Menentukan berat obat (Berdasarkan dosis dan total berat hewan) 𝐷𝑜𝑠𝑖𝑠 × 𝑇𝑜𝑡𝑎𝑙 𝐽𝑢𝑚𝑙𝑎ℎ 𝐵𝑜𝑏𝑜𝑡 𝐻𝑒𝑤𝑎𝑛
= 400 𝑚𝑔/𝐾𝑔𝐵𝐵 × (250 𝑔𝑟 × 5)
= 400 𝑚𝑔/𝐾𝑔𝐵𝐵 × (250 𝑔𝑟 × 5)
= 400 𝑚𝑔/𝐾𝑔𝐵𝐵 × (1250 𝑔𝑟 )
= 400 𝑚𝑔/𝐾𝑔𝐵𝐵 × (1,25 𝐾𝑔)
= 500 𝑚𝑔/𝐾𝑔𝐵𝐵 → 𝐵𝑒𝑟𝑎𝑡 𝑦𝑎𝑛𝑔 𝑑𝑖𝑡𝑖𝑚𝑏𝑎𝑛𝑔
3. Menentukan volume yang disediakan
𝑉𝑜𝑙𝑢𝑚𝑒 𝑆𝑒𝑑𝑖𝑎𝑎𝑛 = 𝐵𝑒𝑟𝑎𝑡 𝐸𝑘𝑡𝑟𝑎𝑘 𝑘𝑜𝑛𝑠𝑒𝑛𝑡𝑟𝑎𝑠𝑖
𝑉𝑜𝑙𝑢𝑚𝑒 𝑆𝑒𝑑𝑖𝑎𝑎𝑛 = 500 𝑚𝑔 ∶ 4000 𝑚𝑔 100 𝑚𝑙
𝑉𝑜𝑙𝑢𝑚𝑒 𝑆𝑒𝑑𝑖𝑎𝑎𝑛 = 500 𝑚𝑔 × 100 𝑚𝑙 4000 𝑚𝑔
𝑉𝑜𝑙𝑢𝑚𝑒 𝑆𝑒𝑑𝑖𝑎𝑎𝑛 = 500 𝑚𝑔 × 100 𝑚𝑙
4000 𝑚𝑔= 12,5 𝑚𝑙
4. Jumlah dosis yang diberikan perhewan
𝐷𝑜𝑠𝑖𝑠 𝑝𝑒𝑚𝑏𝑒𝑟𝑖𝑎𝑛 = 𝐵𝑒𝑟𝑎𝑡 ℎ𝑒𝑤𝑎𝑛 × % 𝑝𝑒𝑚𝑏𝑒𝑟𝑖𝑎𝑛
= 250 × 1 %
= 250 × 1 𝑚𝑙 100 𝑔𝑟
= 2,5 𝑚𝑙 → 𝑑𝑒𝑛𝑔𝑎𝑛 𝑏𝑒𝑟𝑎𝑡 𝑡𝑖𝑘𝑢𝑠 250 𝑔𝑟𝑎𝑚
LAMPIRAN 3
LEMBAR OBSERVASI PENELITIAN
Efek Antiinflamasi Ekstrak Daun Beruwas Laut (Scaevola Taccada (Gaertn)Roxb) Terhadap Kadar IL-6 Pada Mammae Tikus Betina Strain
Sprangue Dawley yang Di Induksi Bakteri Staphylococcus Aureus A. Kelompok Kontrol Negatif (Pemberian terapi menggunakan air putih,
secara oral)
No Kode
Responden
Pengukuran Kadar IL-6 Pengkuran I
(Hari Ke-8)
Pengkuran II (Hari Ke-9)
Pengkuran III (Hari Ke-14)
LEMBAR OBSERVASI PENELITIAN
Efek Antiinflamasi Ekstrak Daun Beruwas Laut (Scaevola Taccada (Gaertn)Roxb) Terhadap Kadar IL-6 Pada Mammae Tikus Betina Strain
Sprangue Dawley yang Di Induksi Bakteri Staphylococcus Aureus B. Kelompok Kontrol Positif (Pemberian terapi menggunakan antibiotik
fluksasosilin, secara oral)
No Kode
Responden
Pengukuran Kadar IL-6 Pengkuran I
(Hari Ke-8)
Pengkuran II (Hari Ke-9)
Pengkuran III (Hari Ke-14)
LEMBAR OBSERVASI PENELITIAN
Efek Antiinflamasi Ekstrak Daun Beruwas Laut (Scaevola Taccada (Gaertn)Roxb) Terhadap Kadar IL-6 Pada Mammae Tikus Betina Strain
Sprangue Dawley yang Di Induksi Bakteri Staphylococcus Aureus C. Kelompok Kontrol Positif (Pemberian terapi menggunakan antibiotik
fluksasosilin + Ekstrak beruwas laut, secara oral)
No Kode
Responden
Pengukuran Kadar IL-6 Pengkuran I
(Hari Ke-8)
Pengkuran II (Hari Ke-9)
Pengkuran III (Hari Ke-14)
Lampiran 4
Proses pengeringan dan maserasi
Uji fitokimia senyawa ekstrak daun beruwas laut
Kultur Bakteri
Pengambilan darah
Proses pengambilan darah
Pemeriksaan Elisa